日本三角涡虫自然状态下的生殖研究

作者１９９０年对六安市某水塘中的日本三角涡虫Ｋｕｇｅｓｉａ ｊａｐｏｎｉｃａ（下文简称涡虫）在自然状态下的生殖进行了近一年的观察研究，并于１９９２年５－６月、１０－１１月进行了采样。每月采标本３－４次，每次１５分钟，并记录水湿。从统计数据得知涡虫每年进行２次有性生殖，分别是４－５月，水温１９－２１℃；９－１０月，水温２０－２４℃。涡虫的断裂生殖３－１１月均可发生，并在５月、１０月出现高峰，其水温分     

涡虫生殖生物学研究进展

涡虫由于具有极强的再生能力而成为发育生物学及再生生物学研究的模式生物。此外,其在有性生殖方面所表现出来的独特性也备受人们关注。目前,涡虫生殖生物学研究领域主要围绕两个热点问题开展工作：1.无性生殖向有性生殖转化的诱因及机制的探讨;2.生殖相关基因的克隆、表达及功能分析。有关生殖转化机制方面的研究主要集中在涡虫的性化相关事件以及性化物质的本质探索;截至目前已克隆并对其表达和功能进行探讨的涡虫生殖相关基因主要有DjPTK1、vasa-like 基因、DeY1、Dryg、nanos相关基因以及Drpiwi-1等。此外,本文也对有关涡虫生殖生物学方面存在的问题及未来该领域的发展趋势进行了总结和展望。     

中国林蛙的生殖量特征及其地理变化

标本采自山西省境内的８个地点。结果表明１研究区域内性成熟雌蛙平均体长范围是３４．５－５２．３ｍｍ,高山森林带种群明显偏高;研究种群雌蛙的体长显著低于东北居群。２平均生殖量的范围是４２２．４－８４０．９枚,个体的最低,最高值分别为３８和１９７８枚,平均相对生殖量的范围是１０．７－１８．０,个体的最低,最高值各为１．３和３４．４。与东北居群相比,生殖量的变异幅度较高。３除高山森林带外,其余调查点的生殖     

中国淡水三角涡虫染色体变化与生殖的关系

利用空气干燥法对淡水三角涡虫的染色体进行研究。结果表明：三角涡虫的染色体数目为n=8,2n=16,2n=24,为二倍体2n=2x=16、三倍体2n=3x=24和混合倍体2n=2x=16,2n=3x=24,有时也可见到非整倍体。以有性生殖为主的类群．有性生殖期间有性个体大量存在,生殖器官比较发达．染色体为二倍体;以无性生殖为主的类群,很少出现有性个体,染色体为三倍体;既有有性生殖又有无性生殖的类群,有性生殖期间,生殖器官发育稍差,染色体主要为混合倍体．有时出现二倍体或三倍体。本文对影响三角涡虫性成熟的因素也进行了讨论。     

涡虫异常生殖过程的观察

涡虫被异物穿插后,出现在咽前断裂的异常无性生殖,及其断裂形成的头部片段不能完全再生成一正常个体的情况。这一特殊现象,可以认为是异物穿插破坏了涡虫体内抑制生长物质正常梯度分布的结果。     

三道眉草Wu繁殖的研究

三道眉草Ｗｕ在山东省的泰山和租徕山,每年繁殖一次,繁殖期在６－７月,每窝产卵多为４枚,孵卵期１１天,育雏期１３天。据１９９２年１月对１８只鸟体的检析,冬季年龄的组成,其成体和幼体为１：１而雌体的只数多于雄体。     

恒定磁场对日本三角涡虫无性横裂生殖及2种抗氧化酶活力的影响

目的:探讨恒定磁场对日本三角涡虫(Dugesia japonica)无性横裂生殖及抗氧化酶活力的影响.方法:应用种群累积培养法,观察了恒定磁场对日本三角涡虫的种群增长、无性横裂生殖及涡虫体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的影响.结果:场强大小在200mT-500mT范围的恒定磁场能促进涡虫的无性横裂生殖,但恒定磁场显著抑制涡虫的SOD及CAT活力.结论:日本三角涡虫对恒定磁场的生理学响应较为敏感,可作为生物磁学研究适宜的实验材料.     

荒漠沙蜥繁殖生态研究

荒漠沙蜥（Ｐｈｒｙｎｏｃｅｐｈａｌｕｓｐｒｚｅｗａｌｓｋｉｉ）１年繁殖１次,有明显的季节性周期。４月初出蛰时雄性精巢已相当发育,５月其重量和体积最大,６月变小,进入精子发生的晚期,７月降到最小,８月开始回升,１０月中旬入蛰时已接近春季出蛰时水平。１年内仅７月精巢中无精子。雌性出蛰后卵细胞开始发育,４月下旬进入成熟期,６月达高峰,７月下降,８月进入休止期。每雌年产卵１～３枚或１～４枚,平均１．８３（ｎ＝４８,１９８７）和２．１７（ｎ＝４８,１９９３）。特定体长组的生殖率是影响种群年平均生殖率的主要因素。生殖周期与光周期相符,光周期是影响荒漠沙蜥生殖周期的主要气候因子。     

深圳湾大弹涂鱼种群结构与生殖特征

本文探讨了深圳湾大弹涂鱼种群结构和生殖特征。结果表明：该种群以１、２龄鱼组成为主,３龄鱼极少,表明种群结构简单,体长与体重的相关曲线方程为Ｗ＝４．１６１８×１０－５Ｌ２．７４５１（ｒ＝０．９５７６）。雌雄性比为３：２。一龄鱼开始性成熟。雌鱼性成熟最小生物学体长为６２ｍｍ,雄鱼为５９ｍｍ。个体绝对生殖力波动于１９５６—１４０５３粒,平均７２９３粒;个体相对生殖力Ｆ／Ｌ：２９．５—１００．５粒／ｍｍ,平均７９粒／ｍｍ;Ｆ／Ｗ：５１１．０—８０４．９粒／ｇ,平均７５２粒／ｇ。４—６月为繁殖盛期,７—８月为繁殖末期。雌性周年月平均成熟系数（ＧＳＩ）变动范围为０．２７％—４．３６％。大弹涂鱼在一个生殖季节中只能产卵一次,属于一次性排卵类型。     

无融合生殖

自1745年博内(Bonnet)在研究蚜虫中发现孤雌生殖以来,世界各地的植物学家,也在众多的植物中发现了这种奇特的生殖现象,迄今为止,已记录到36科300余种植株的孤雌生殖。为同有性生殖和无性生殖相区分,植物学家把它定名为无融合生殖。 由于无融合生殖生理机制十分复杂,长期以来,对其概念的界定,植物学界一直没有统一。我国学者蔡得田和陈冬玲,在1993年提出:无融合生殖是指发生在植物胚珠中,不经过精卵融合形成胚,以种子进行繁殖的生殖方式。此界定包括三部分内容,一是它与无性生殖明显的区别是发生在性器官中;二是没有性融合过程而区别于有性生殖;三是以种子繁殖     

壳聚糖酶的克隆表达与壳寡糖的制备分析

目的:克隆壳聚糖酶基因于大肠杆菌中实现高表达,制备壳寡糖。方法:以枯草芽孢杆菌总DNA为模板扩增壳聚糖酶基因(CSN),克隆至载体pET23a(+)上,转化菌株BL21(DE3)。重组子经0.5 mmol/L IPTG诱导后,SDS-PAGE和质谱检测与鉴定重组酶。酶纯化后水解壳聚糖,薄层色谱分析其水解产物。结果:质谱证明壳聚糖酶(31.5kDa)成功表达,表达量占菌体总蛋白的45%左右。纯化后重组酶浓度为900 mg/L,纯度95%、回收率85%,酶活力为10 000 U/mg。壳聚糖降解产物为壳二糖至壳四糖。结论:原核表达载体pET23a(+)-CSN构建正确,壳聚糖酶表达量与活性高,适用于水解壳聚糖制备壳寡糖。     

国内外蝗害治理技术现状与展望

本文首先概述了国内外蝗虫发生与为害的态势,总结了现阶段我国蝗虫发生与为害的主要特点:即农田飞蝗暴发频繁而且严重,草原土蝗的发生时常造成严重的经济损失,而且侵入城市干扰市民生活,我国与周边国家之间蝗虫过境迁移频繁,使用化学农药污染环境和农产品;分析了国内外蝗虫防治对策与技术的发展现状,重点介绍了应急防治和可持续治理对策、...     

Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission

The molecular characterisation of species and genotypes of Cryptosporidium and Giardia is essential for accurately identifying organisms and assessing zoonotic transmission. Results of recent molecular epidemiological studies strongly suggest that zoonotic transmission plays an important role in cryptosporidiosis epidemiology. In such cases the most prevalent zoonotic species is Cryptosporidium parvum. Genotyping and subtyping data suggest that zoonotic transmission is not as prevalent in the epidemiology of giardiasis. Molecular characterisation of Cryptosporidium and Giardia is a relatively recent application that is evolving as new genes are found that increase the accuracy of identification while discovering a greater diversity of species and yet unnamed taxa within these two important genera. As molecular data accumulate, our understanding of the role of zoonotic transmission in epidemiology and clinical manifestations is becoming clearer.     

Synthesis and turnover of cerebrosides and phosphatidylserine of myelin and microsomal fractions of adult and developing rat brain.

The synthesis and turnover of cerebrosides and phospholipids was followed in microsomal and myelin fractions of developing and adult rat brains after an intracerebral injection of [U-14C]serine. The kinetics of incorporation of radioactivity into microsomal and myelin cerebrosides indicate the possibility of a precursor-product relationship between cerebrosides of these membranes. The specific radioactivity of myelin cerebrosides was corrected for the deposition of newly formed cerebrosides in myelin. Multiphasic curves were obtained for the decline in specific radioactivity of myelin and microsomal cerebrosides, suggesting different cerebroside pools in these membranes. The half-life of the fast turning-over pool of cerebrosides of myelin was 7 and 22 days for the developing and adult rat brain respectively. The half-life of the slowly turning-over pool of myelin cerebrosides was about 145 days for both groups of animals. The half-life of the rapidly turning-over microsomal cerebrosides was calculated to be 20 and 40 h for the developing and adult animals respectively. The half-life of the intermediate and slowly turning-over microsomal cerebrosides was 11 and 60 days respectively, for both groups of animals. The amount of incorporation of radioactivity into microsomal cerebrosides from L-serine was greatly decreased in the adult animals, and greater amounts of the precursor were directed towards the synthesis of phosphatidylserine. In the developing animals, considerable amounts of cerebrosides were synthesized from L-serine, besides phosphatidylserine. The time-course of incorporation indicated that a precursor-product relationship exists between microsomal and myelin phosphatidylserine. The half-life of microsomal phosphatidylserine was calculated to be about 8 h for the fast turning-over pool in both groups of animals.     

白术同源四倍体的诱导和鉴定及其与二倍体过氧化物酶的比较

以白术（Atractylodes macrooephala Koidz.）二倍体组培苗为材料,对其四倍体诱导方法进行研究,共获得45个白术同源四倍体株系,为优良株系的选育提供了材料。此外,还分析比较了其中8个白术四倍体株系与二倍体的过氧化物酶同工酶（POD）的酶谱差异,发现四倍体各株系过氧化物酶同工酶谱比二倍体的均多了Rf0.310的谱带,且总过氧化物酶比活力也发生了很大改变,对探讨白术四倍体优良株系的生理生化机理具有一定的参考价值。     

Identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats

Enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs Ent. hirae was found almost as often as Ent. faecalis. Most Ent.faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. Typical Ent. avium as well as atypical Ent. avium -like strains were seen in dogs, while the related species Ent. raffinosus was associated with cat tonsils. Enterococcus cecorum also occurred mainly in cats. Certain atypical strains, presumptively identified as Ent. cecorum , shared characteristics with Ent. columbae.
The most frequent streptococcal species in tonsils of cats and dogs were Streptococcus suis and Strep. canis. Streptococcus canis and Strep. bovis predominated in anal swabs. The canine Strep. suis differed from the common porcine strains in fermenting mannitol.
Forty-seven of the 288 isolates examined could not be identified or related to known species. The characteristics of two groups of these bacteria, provisionally called 'Ton 31 group' and 'O7 group' are described.