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Specific DNA binding of GAL4, a positive regulatory protein of yeast 总被引:173,自引:0,他引:173
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GAL4 activates gene expression in mammalian cells 总被引:73,自引:0,他引:73
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Activating regions of yeast transcription factors must have both acidic and hydrophobic amino acids 总被引:4,自引:0,他引:4
Douglas M. Ruden 《Chromosoma》1992,101(5-6):342-348
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In vivo DNA-binding properties of a yeast transcription activator protein. 总被引:30,自引:17,他引:13
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GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators 总被引:24,自引:0,他引:24
A mutant yeast in which a weak GAL4-derived activator functions as a strong activator bears a single mis-sense mutation in GAL11 (a.k.a. SPT13). The first 74 amino acids of GAL4, including the zinc-dependent DNA binding region, attached to an acidic activating sequence, are sufficient to respond both to GAL11 and to our mutant GAL11P (potentiator). PPR1, a yeast activator with a similar zinc finger sequence, also responds to GAL11 and to GAL11P, whereas regulators bearing unrelated DNA binding motifs do not. GAL11 itself works as a strong activator when tethered to DNA by fusion to the bacterial LexA protein, and deletion of GAL11 is known to cause a 5- to 10-fold reduction in GAL4 activity. We suggest that a complex of GAL4 and GAL11 constitutes a particularly strong activator; evidence that the putative GAL4-GAL11 complex ordinarily forms preferentially on DNA suggests a biological rationale for GAL11 action. 相似文献
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