Temperature effects of interspecies competition between cladoceran species in experimental conditions

In experiments with mono- and polycultures the hypothesis that small-size cladoceran species (Diaphanosoma brachyurum Lievin and Ceriodaphnia reticulata Jurine) are more successful at higher temperatures whereas large-size species (Daphnia magna Straus and Simocephalus vetulus O.F. Müller) under low temperatures was tested. The biomass of Simocephalus vetulus exceeded the biomass of the other species both in mono- and polycultures. An increase of temperature from 18 up to 25°C led to reduction of the biomass of all species. The largest species, D. magna, responded to higher temperature by increased mortality. We assume that the reduction of the biomass of the large species allowed the small C. reticulata to develop successfully in mixed cultures at higher temperature; however it was completely eliminated at lower temperature. Apparently, the survival strategy of large cladocerans involves delayed juvenile development at lower temperatures. However, at higher temperatures, juvenile development of large species accelerates, and their mortality increases. Individual strategies of the studied species to survive at different temperatures are analyzed. On the whole the increase of temperature can result in decrease of the biomass of cladocerans and prevailence of small-size species over large-size ones.     

Effect of water exchange rate on interspecies competition between submerged macrophytes: functional trait hierarchy drives competition

Plant and Soil - For well over a century, rhizobia have been recognized as effective biofertilizer options for legume crops. This has led to the widespread use of rhizobial inoculants in...     

Plant interspecies competition for sunlight: a mathematical model of canopy partitioning

We examine the influence of canopy partitioning on the outcome of competition between two plant species that interact only by mutually shading each other. This analysis is based on a Kolmogorov-type canopy partitioning model for plant species with clonal growth form and fixed vertical leaf profiles (Vance and Nevai in J. Theor. Biol., 2007, to appear). We show that canopy partitioning is necessary for the stable coexistence of the two competing plant species. We also use implicit methods to show that, under certain conditions, the species’ nullclines can intersect at most once. We use nullcline endpoint analysis to show that when the nullclines do intersect, and in such a way that they cross, then the resulting equilibrium point is always stable. We also construct surfaces that divide parameter space into regions within which the various outcomes of competition occur, and then study parameter dependence in the locations of these surfaces. The analysis presented here and in a companion paper (Nevai and Vance, The role of leaf height in plant competition for sunlight: analysis of a canopy partitioning model, in review) together shows that canopy partitioning is both necessary and, under appropriate parameter values, sufficient for the stable coexistence of two hypothetical plant species whose structure and growth are described by our model. A. L. Nevai was supported in part by the National Institutes of Health, National Research Service Award (T32-GM008185) from the National Institute of General Medical Sciences (NIGMS).     

猎物密度对七星瓢虫与异色瓢虫种间竞争的影响

通过单种群与混合种群饲养,研究了猎物密度对七星瓢虫Coccinella septempunctata和异色瓢虫Leis axyridis种间竞争影响,并用Lotka-Volterra种间竞争模型对两种瓢虫在猎物相对充足与不足条件下的种间竞争进行模拟,结果表明：猎物充足,两种瓢虫的种群增长呈线性增长趋势;猎物不足时,单独饲养时,两种瓢虫的种群增长趋势呈Logistic曲线,混合饲养时异色瓢虫的种群增长呈上升趋势,七星瓢虫趋于下降。在两种瓢虫的种间竞争中,异色瓢虫占相对优势,竞争的结局是二者可以形成一个稳定的平衡局面而得以共存。     

Function of the pyruvate oxidase-lactate oxidase cascade in interspecies competition between Streptococcus oligofermentans and Streptococcus mutans

Complex interspecies interactions occur constantly between oral commensals and the opportunistic pathogen Streptococcus mutans in dental plaque. Previously, we showed that oral commensal Streptococcus oligofermentans possesses multiple enzymes for H(2)O(2) production, especially lactate oxidase (Lox), allowing it to out-compete S. mutans. In this study, through extensive biochemical and genetic studies, we identified a pyruvate oxidase (pox) gene in S. oligofermentans. A pox deletion mutant completely lost Pox activity, while ectopically expressed pox restored activity. Pox was determined to produce most of the H(2)O(2) in the earlier growth phase and log phase, while Lox mainly contributed to H(2)O(2) production in stationary phase. Both pox and lox were expressed throughout the growth phase, while expression of the lox gene increased by about 2.5-fold when cells entered stationary phase. Since lactate accumulation occurred to a large degree in stationary phase, the differential Pox- and Lox-generated H(2)O(2) can be attributed to differential gene expression and substrate availability. Interestingly, inactivation of pox causes a dramatic reduction in H(2)O(2) production from lactate, suggesting a synergistic action of the two oxidases in converting lactate into H(2)O(2). In an in vitro two-species biofilm experiment, the pox mutant of S. oligofermentans failed to inhibit S. mutans even though lox was active. In summary, S. oligofermentans develops a Pox-Lox synergy strategy to maximize its H(2)O(2) formation so as to win the interspecies competition.     

The role of innate immune responses in the outcome of interspecies competition for colonization of mucosal surfaces

Since mucosal surfaces may be simultaneously colonized by multiple species, the success of an organism may be determined by its ability to compete with co-inhabitants of its niche. To explore the contribution of host factors to polymicrobial competition, a murine model was used to study the initiation of colonization by Haemophilus influenzae and Streptococcus pneumoniae. Both bacterial species, which occupy a similar microenvironment within the nasopharynx, persisted during colonization when given individually. Co-colonization, however, resulted in rapid clearance of S. pneumoniae from the upper respiratory tract, associated with increased recruitment of neutrophils into paranasal spaces. Systemic depletion of either neutrophil-like cells or complement was sufficient to eliminate this competitive effect, indicating that clearance was likely due to enhanced opsonophagocytic killing. The hypothesis that modulation of opsonophagocytic activity was responsible for host-mediated competition was tested using in vitro killing assays with elicited neutrophil-like cells. Components of H. influenzae (but not S. pneumoniae) stimulated complement-dependent phagocytic killing of S. pneumoniae. Thus, the recruitment and activation of neutrophils through selective microbial pattern recognition may underlie the H. influenzae-induced clearance of S. pneumoniae. This study demonstrates how innate immune responses may mediate competitive interactions between species and dictate the composition of the colonizing flora.     

The xnp1 P2-like tail synthesis gene cluster encodes xenorhabdicin and is required for interspecies competition

Xenorhabdus nematophila, the mutualistic bacterium of the nematode Steinernema carpocapsae, produces the R-type bacteriocin called xenorhabdicin, which is thought to confer a competitive advantage for growth in the insect host. We have identified a P2-like tail synthesis gene cluster (xnp1) that is required for xenorhabdicin production. The xnp1 genes were expressed constitutively during growth and were induced by mitomycin C. Deletion of either the sheath (xnpS1) or fiber (xnpH1) genes eliminated xenorhabdicin production. Production of R-type bacteriocins in a host organism had not been shown previously. We show that xenorhabdicin is produced in the hemocoel of insects infected with the wild type but not with the ΔxnpS1 deletion strain. Xenorhabdicin prepared from the wild-type strain killed the potential competitor Photorhabdus luminescens TT01. P. luminescens was eliminated during coculture with wild-type X. nematophila but not with the ΔxnpS1 strain. Furthermore, P. luminescens inhibited reproduction of S. carpocapsae in insect larvae, while coinjection with wild-type X. nematophila, but not the ΔxnpS1, strain restored normal reproduction, demonstrating that xenorhabdicin was required for killing P. luminescens and protecting the nematode partner. Xenorhabdicin killed X. nematophila from Steinernema anatoliense, demonstrating for the first time that it possesses intraspecies activity. In addition, activity was variable against diverse strains of Xenorhabdus and Photorhabdus and was not correlated with phylogenetic distance. These findings are discussed in the context of the role of xenorhabdicin in the life cycle of the mutualistic bacterium X. nematophila.     

人体肠道细菌群落与疾病

肠道定植有100万亿细菌,这占到了人体细菌总量的绝大多数。一旦肠道菌群失调,就会产生一系列疾病。本文介绍了人体肠道细菌群落异常与5种肠道疾病和5种肠道外疾病的关系,并推荐用益生菌和益生素来治疗人体肠道细菌群落异常。为了解人体肠道细菌群落和人体健康的关系,美国国立卫生研究院已启动了人类微生物组计划,欧洲委员会也正在资助人类肠道宏基因组学项目,而中国在此项目中亦取得了可喜进步。基于肠道宏基因组的个体化医疗时代已不再遥远。     

Human diseases of the SSU processome

Ribosomes are the cellular machines responsible for protein synthesis. Ribosome biogenesis, the production of ribosomes, is a complex process involving pre-ribosomal RNA (rRNA) cleavages and modifications as well as ribosomal protein assembly around the rRNAs to create the functional ribosome. The small subunit (SSU) processome is a large ribonucleoprotein (RNP) in eukaryotes required for the assembly of the SSU of the ribosome as well as for the maturation of the 18S rRNA. Despite the fundamental nature of the SSU processome to the survival of any eukaryotic cell, mutations in SSU processome components have been implicated in human diseases. Three SSU processome components and their related human diseases will be explored in this review: hUTP4/Cirhin, implicated in North American Indian childhood cirrhosis (NAIC); UTP14, implicated in infertility, ovarian cancer, and scleroderma; and EMG1, implicated in Bowen–Conradi syndrome (BCS). Diseases with suggestive, though inconclusive, evidence for the involvement of the SSU processome in their pathogenesis are also discussed, including a novel putative ribosomopathy. This article is part of a Special Issue entitled: Role of the Nucleolus in Human Disease.     

Catatonia as an element of submissive behavior in mice during interspecies agonistic interactions

A catatonia-like state was elicited in male mice with different experience of social interactions, by pinch of scruff of the neck in a suspended state. In submissive males with long experience of defeat the total time of catatonia reaction during test is considerably greater than in aggressive individuals with a long experience of victories. The change of the social status of the individuals resulting from agonistic interactions leads to a change of the catatonia reaction: manifestation of submissive behaviour is accompanied by an increase of total time of catatonia reaction in the test, the aggressive behaviour is accompanied by its decrease.     

Mechanisms responsible for the development of periphyton community structure during seasonal succession: the role of interspecies competition and plankton sedimentation

The development of periphyton community structure by exchange of organisms between substratum and water column (noninteractive mechanism) and by interspecific competition for surface (interactive mechanism) was studied during seasonal succession in Akulovsky water supply channel (the Upper Volga basin). The influence of exchange was assumed by similarity between the species composition of plankton and periphyton. At early stages of succession when the diatoms dominated in periphyton the community was formed mainly by phytoplankton sedimentation, while the competition for substratum didn't result in decrease of species diversity because the poor competitors were partly displaced by new colonists from the water column. Later when the green filamentous algae abundantly developed in periphyton, their numbers were probably controlled by factors not related to exchange of propagules. At the same time, the species structure of secondary periphyton cover developing on the thallus of filamentous algae depended mainly on the plankton sedimentation. At the last stages of seasonal succession when periphyton was represented by colonies of cyanobacteria and diatoms closely covering the substratum, the exchange of organisms between substratum and water column was not so important as interspecific competition for surface. As one could suppose, increase in biomass in this period resulted in the decrease of specificity as it was predicted by hypothesis of interactive community. In such a way, both mechanisms (interactive and noninteractive ones) took part in development of periphyton structure. Their relative influence changed in the course of seasonal succession.     

Studies on the interspecies competition between the azuki bean weevil and the southern cowpea weevil IV. Competition between strains

This study analyzes results of interspecies competition between the azuki bean weevil, Callosobruchus chinensis and the southern cowpea weevil, C. maculatus using the four geographical strains of each species.

1. Fifteen combinations of one strain from each species were set up to compete. The strains of C. chinensis, rather than of C. maculatus, characteristically determine the eventual outcomes of competition. Some strains of C. chinensis (jC and kC) invariably won every encounter with C. maculatus. Another strain (nC) always lost its encounters, while one other strain (iC) won some encounters (:cQ and :cQ) and lost others (:tQ and :bQ). For any combination the competition results were deterministic and no reversal in outcome was observed among the replicates.
2. The weakness of strain nC in competition is explained by its characteristics in single species populations, different greatly from other strains of C. chinensis, although it had the similar primary (individual) characteristics as other strains of C. chinensis. Strain iC had the shortest adult longevity in the four strains of C. chinensis although it behaved similar to strain jC at the single species population level. Especially under the food supply schedule of the present experiment the shortness of adult longevity might be the important factor determining the outcome.
3. The relationship of the eight strains based on the individual and single species population characteristics, and that based on the competition results were very similar. Even when the competition results could not be explained by any one or two of these characteristics alone, the outcome would be largely decided by the overall biotic profile made by several characteristics.

     

Semen displacement as a sperm competition strategy

Using a sample of 652 college students, we examined several implications of the hypothesis that the shape of the human penis evolved to enable males to substitute their semen for those of their rivals. The incidence of double mating by females appears sufficient to make semen displacement adaptive (e.g., one in four females acknowledge infidelity, one in eight admit having sex with two or more males in a 24-hour period, and one in 12 report involvement in one or more sexual threesomes with two males). We also document several changes in post-ejaculatory behavior (e.g., reduced thrusting, penis withdrawal, loss of an erection) which may have evolved to minimize displacement of the male’s own semen. Consistent with predictions derived from a theoretical model (Gallup and Burch 2006), we discovered that most females report waiting at least 48 hours following an instance of infidelity before resuming sex with their in-pair partners.     

Human diseases associated with connexin mutations

Gap junctions and hemichannels comprised of connexins impact many cellular processes. Significant advances in our understanding of the functional role of these channels have been made by the identification of a host of genetic diseases caused by connexin mutations. Prominent features of connexin disorders are the inability of other connexins expressed in the same cell type to compensate for the mutated one, and the ability of connexin mutants to dominantly influence the activity of other wild-type connexins. Functional studies have begun to identify some of the underlying mechanisms whereby connexin channel mutation contributes to the disease state. Detailed mechanistic understanding of these functional differences will help to facilitate new pathophysiology driven therapies for the diverse array of connexin genetic disorders. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve.     

Adhesion as a weapon in microbial competition

Microbes attach to surfaces and form dense communities known as biofilms, which are central to how microbes live and influence humans. The key defining feature of biofilms is adhesion, whereby cells attach to one another and to surfaces, via attachment factors and extracellular polymers. While adhesion is known to be important for the initial stages of biofilm formation, its function within biofilm communities has not been studied. Here we utilise an individual-based model of microbial groups to study the evolution of adhesion. While adhering to a surface can enable cells to remain in a biofilm, consideration of within-biofilm competition reveals a potential cost to adhesion: immobility. Highly adhesive cells that are resistant to movement face being buried and starved at the base of the biofilm. However, we find that when growth occurs at the base of a biofilm, adhesion allows cells to capture substratum territory and force less adhesive, competing cells out of the system. This process may be particularly important when cells grow on a host epithelial surface. We test the predictions of our model using the enteric pathogen Vibrio cholerae, which produces an extracellular matrix important for biofilm formation. Flow cell experiments indicate that matrix-secreting cells are highly adhesive and form expanding clusters that remove non-secreting cells from the population, as predicted by our simulations. Our study shows how simple physical properties, such as adhesion, can be critical to understanding evolution and competition within microbial communities.