Studies in the taxonomy of the Polypores II

The following genera are redefined:Albatrellus S. F. Gray,Heterobasidion Bref.,Haploporus Bond. et Sing. ex Sing.,Fomitopsis P. Karst. andRigidoporus Murrill two new subgenera are described:Polyporus subgen.Dendropolyporus Pouz. (type:Polyporus umbellatus) andRigidoporus subgen.Neooxyporus Pouz. (type:Polyporus latemarginatus); the genusOxyporus (Bourd. etGalz.)Donk is classified as a subgenus of the genusRigidoporus,Murrill and the generaBjerkandera P. Karst. andLeptoporus quél. are classified as subgenera of the genusTyromyces P. Karst. The new subfamilyAlbatrelloideae Pouz. (genera:Albatrellus andGrifola) is described and 14 new specific combinations are made. The new genusIrpicodon Pouz. (type:Irpex pendulus) is proposed.     

The variability of critical species ofLotus L. in Iran and in the neighbouring countries I. the circle ofL. corniculatus L. andL. gebelia Vent.

The region of Iran, Iraq, Afghanistan and the neighbouring countries is important for some groups of the speciesLotus L., especially those of the circle ofL. corniculatus L. andL. gebelia Vent. The first group is represented by the speciesL. corniculatus L. with 4 subspecies (3 of which are important for this region), andL. tenuis Waldst. etKit. which here attains the eastern boundary of the continuous area of distribution, and by the eastern speciesL. krylovii Schischk. etSerg. andL. rechingeri Chrtková-?ertová. The second group is represented by the speciesL. gebelia Vent.,L. michauxianus Ser. in DC. andL. libanoticus Boiss. their areas of distribution covering mostly those regions. Most of the species show considerable variability within the species.     

Differentiation of species in Gonioloboceras

The type specimen ofGonioloboceras goniolobum (Meek), rediscovered by Spath in the British Museum, is the foundation for a more accurate comparative study of this and other species ofGonioloboceras.Gonioloboceras described asG. goniolobum byElias in 1938 is differentiated asGonioloboceras schmidti, new species. Suture sets (new term) for several growth stages inG. goniolobum (Meek),G. welleriSmith,G. schmidtiElias, G.eliasiMiller &Owen, andG. asiaticumLibrovitch are assembled and used for differentiation of the species.The Kazakhstan goniatite faunule containingG. asiaticum is considered of very late Pennsylvanian age.     

Glacial relics in the bryoflora of the highlands Českomoravská vrchovina (Bohemian-Moravian Highlands); Their habitat and cenotaxonomic value

The paper sums up the information which the author has so far collected about the habitats and phytocenotic conditions of the glacial relics ofScorpidium scorpioides (Hedw.) Limpr.,Calliergon trifarium (Web. etMohr.) Kindb.,Paludella squarrosa (Hedw.) Bred., andMeesia triquetra (Hook. etTayl.) Aongstr. in the Highlands ?eskomoravská vrchovina (Bohemian-Moravian Highlands) in Czechoslovakia. Simultaneously, it presents a survey of the localities in which the occurrence of these species has been ascertained up to now.     

Beiträge zur Sedimentation und fossilführung des hunsrückschiefers 14.Viriatellina fuchsi (Kutscher, 1931) im Hunsrückschiefer und im Tentaculitenknollenkalk thüringens

The new genusViriatellina Bou?ek, 1964 unites the two older species namedNovakia gemündina Runzheimer, 1932 andTentaculites fuchsi Kutscher, 1931. ThusViriatellina gemuendina (Runzheimer) is cancelled, on the other handViriatellina fuchsi (Kutscher) remains valid. RecentlyViriatellina fuchsi (Kutscher) has been found in the tentaculitiferous limestone of Thuringia.     

Boreal forest dynamics in north-eastern Sweden during the last 10,000 years based on pollen analysis

A pollen record obtained from a 2.2-m sediment succession deposited in a small lake in the province of Västerbotten, north-eastern Sweden, reveals the presence of continuous forest cover since 8,500 calendar years before present (cal b.p.). Forest with abundant Pinus (pine) and Betula (birch) initially colonized the area, followed by a dominance of deciduous trees, primarily Betula, from ca. 8,000 to ca. 3,200 cal b.p. Pollen accumulation rates of Quercus (oak), Ulmus (elm) and Tilia (linden) suggest the possible local presence of these thermophilous tree species during this period. The climate gradually became colder and moister around 3,500 cal b.p. and an increased abundance of Sphagnum spores indicates paludification. Picea (spruce) became established around 3,200 cal b.p. and less than 500 years later this was the dominant tree species around the lake. The fire frequency as inferred from charcoal particles exhibits a general increase from ca. 3,000 cal b.p. with subsequent charcoal accumulation maxima at around 2,800 cal b.p., 1,700 cal b.p. and in recent time. The human influence on vegetation was significant during the last 200–300 years. Soil erosion increased substantially and fern spores amount to ca. 55% of the total pollen assemblage in the uppermost samples. These results suggest an extensive anthropogenic impact on the local forest ecosystem, with abundant logging, burning and ditching in the vicinity of the lake. Independent evidence of sub-recent human-induced environmental change is provided by historical accounts. Complementary information on catchment soil development and aquatic nutrient status was provided by records of magnetic susceptibility and elemental carbon, and nitrogen contents obtained from the same sediment core.     

Glacial relics in the bryoflora of the highlands Českomoravská vrchovina (Bohemian-Moravian Highlands); Their habitat and cenotaxonomic value

The paper sums up the information which the author has so far collected about the habitats and phytocenotic conditions of the glacial relics ofScorpidium scorpioides (Hedw.) Limpr.,Calliergon trifarium (Web. etMohr.)Kindb.,Paludella squarrosa (Hedw.) Brid., andMeesia triquetra (Hook. etTayl.)Aongstr. in the Highlands ?eskomoravská vrchovina (Bohemian-Moravian Highlands) in Czechoslovakia. Simultaneously, it presents a survey of the localities in which the occurrence of these species has been ascertained up to now.     

New subfamily Proschizophoriinae of dalmanellid Brachiopds

Proschizophoria Maillieux is restudied and found to be related to the Silurian genusIdiorthis McLearn and a new genusCordatomyonia, type speciesCordatomyonia shupei, n. sp.Proschizophoria, Idiorthis, andCordatomyonia are united in the new subfamily Proschizophoriinae which is assigned to the dalmanellid family Rhipidomellidae. Rhipidomellidae and its included subfamilies Heterorthinae, Rhipidomellinae, and Proschizophoriinae are emended and newly diagnosed. A new dalmanellid genus,Megasalopina, is proposed for the Lower Devonian species“Dalmanella” elevata Williams & Breger and its allies. It is thought to have been derived from the draboviinid genusSalopina Boucot and therefore belongs to the Schizophoriidae.Dalmanellopsis Khalfin is removed from the synonymy ofLevenea and is shown to be related toSalopina.     

Karyo-taxonomic study of the genus<Emphasis Type="Italic">Pseudolysimachion</Emphasis> (<Emphasis Type="Italic">Scrophulariaceae</Emphasis>) in the Czech Republic and Slovakia

Flow cytometry was used to determine ploidy levels in the Czech and Slovak taxa of the genusPseudolysimachion (W.D.J. Koch)Opiz (=Veronica auct. p.p.,Scrophulariaceae). In total, 123 populations from the Czech Republic, Slovakia, Ukraine (one locality), Austria (one locality) and Hungary (one locality) were analyzed. InP. maritimum (L.)Á. Löve etD. Löve andP. spicatum (L.)Opiz, two cytotypes were found: diploid (2n=2x=34) and tetraploid (2n=4x=68). In both species the tetraploid cytotype predominated (P. maritimum: 41 tetraploid populations out of 45;P. spicatum: 57 tetraploid populations out of 58). The two cytotypes ofP. maritimum have no taxonomic significance because ploidy level is not obviously correlated with morphology, distribution pattern or ecology. Tetraploid populations ofP. spicatum belong to two morphologically different subspecies, subsp.spicatum and subsp.fischeri Trávní?ek. The diploid cytotype (one population only) should be provisionally classified as a third subspecies ofP. spicatum, which is morphologically similar to the Asian subsp.porphyrianum (Pavlov)Trávní?ek. Only diploid plants (2n=2x=34) ofP. orchideum (Crantz)Wraber were found; all 13 populations that were analyzed belong toP. orchideum s.str. One diploid population sample ofP. spurium subsp.foliosum (Waldst. etKit.)Holub (2n=2x=34) and one tetraploid sample ofP. incanum subsp.pallens (Host)Trávní?ek (2n=4x=68) were also analyzed. In addition, three tetraploid populations of hybrid origin were investigated:P. maritimum ×P. spicatum subsp.spicatum (one population) andP. maritimum ×P. spurium subsp.foliosum (two populations). While hybrid plants ofP. maritimum ×P. spicatum arose from tetraploid parental species, plants ofP. maritimum ×P. spurium probably resulted from a cross between tetraploidP. maritimum and diploidP. spurium. The putative origin and evolutionary importance of polyploids in thePseudolysimachion are discussed.     

Flechtensystematische Studien IV. Die GattungGyalidea Lett

Im Artikel wird die ursprüngliche Gattungsdiagnose derGyalidea Lett. um die Merkmale der Pilzkomponente ergänzt. Die angenommenen verwandtschaftlichen Beziehungen derGyalidea zu der FamilieAsterothyriaceae werden diskutiert. Alle bisher festgestellten Arten mit Übersichten der revidierten Proben werden kurz beschrieben. Neue Taxa und Kombinationen:Gyalidea dodgei spec. n.,G. epiphylla spec. n.,G. mayaguezensis spec. n.,G. portoricensis spec. n.,G. fritzei (Stein) comb. n.,G. fritzei var.rivularis (Eitn.) comb. n.,G. hyalinescens (Nyl.) comb. n.,G. lecideopsis var.convarians (Nyl.) comb. n.,G. lecideopsis var.stigmatoides (Nyl.) comb. n.,G. mexicana (B. de Lesd.) comb. n. undG. subscutellaris (Vězda) comb. n.     

Holothurien-Sklerite aus dem Dachsteinkalk (Ober-Trias) der nördlichen Kalkalpen

By dissolving Dachstein-limestone with acetic acid, Holothurian sclerites were obtained, i. e. sieve plates and wheels. One new species of the genusCalclamna, two new species of the genusCalclamnella, and the well known speciesFissobractites subsymmetrica Kristan-Tollmann were received within the family Calclamidae. For the first time the family Priscopedatidae is represented in the Triassic by two new species of the genusPriscopedatus and byStaurocumites cf.bartensteini Deflandre-Rigaud. Within the family Theeliidae four new species can be adjoined to the genusTheelia whileAcanthotheelia spinosa Frizzell & Exline could be confirmed by comparison.     

Late Pleistocene and Holocene palaeoenvironmental changes in central Tierra del Fuego (~54°S) inferred from pollen analysis

A pollen record was obtained from a coring site at La Correntina mire (54°33′S, 66°59′W, 206 m a.s.l.) to the east of Lago Fagnano, centre of Tierra del Fuego. The results indicate that the valley bottom was free of ice shortly before 15,400 cal bp. Pioneer vegetation included dwarf shrub heaths, grasses and herbs with sparsely distributed Nothofagus trees, indicative of dry conditions. Nothofagus expanded by 10,000 cal bp and the forest-steppe ecotone was established by 9,400 cal bp, implying warm conditions and an increase in available moisture. After ca. 5,000 cal bp, the development of a closed-canopy forest is interpreted as the result of wetter and colder conditions. After 3,000 cal bp, Nothofagus forest became more open, and by about 400 cal bp there was a further decline of the forest. A closed-canopy Nothofagus forest re-established after 400 cal bp.     

Soluble carbohydrates in developing and mature diaspores of polar Caryophyllaceae and Poaceae

The accumulation of soluble carbohydrates in maturing diaspores of flowering plants comprising Arctic populations of Cerastium alpinum, indigenous Antarctic species Colobanthus quitensis and Deschampsia antarctica, and cosmopolitan Poa annua from the Antarctic was investigated. For comparative purposes, the diaspores of two species of flowering plants growing in the area of Olsztyn (Poland), Poa annua (Poaceae) and Cerastium arvense (Caryophyllaceae) were used. A qualitative and quantitative analysis of soluble carbohydrates conducted by means of high-resolution gas chromatography showed that monosaccharides (glucose and fructose), maltose and sucrose, raffinose, myo-inositol and galactinol are ubiquitous in developing and mature diaspores among investigated species. Moreover, D. antarctica and P. annua caryopses additionally contained stachyose and 1-kestose; the seeds of Caryophyllaceae studied were found to contain d-pinitol and d-ononitol. The development and maturation of the seeds of polar Caryophyllaceae and Poaceae were accompanied by the changes in the concentration of their soluble carbohydrates. During maturation, seeds accumulated galactinol and raffinose family of oligosaccharides (RFOs), except C. quitensis. Although seeds of the studied Caryophyllaceae contained d-pinitol and lower amounts of d-ononitol, they did not accumulate α-d-galactoside derivatives of mentioned cyclitols. P. annua caryopses, occurring in the Antarctic, were found to accumulate considerably higher amounts of sucrose and 1-kestose than those developed in Olsztyn.     

Importing statistical measures into Artemis enhances gene identification in the <Emphasis Type="Italic">Leishmania</Emphasis> genome project

Background

Seattle Biomedical Research Institute (SBRI) as part of the Leishmania Genome Network (LGN) is sequencing chromosomes of the trypanosomatid protozoan species Leishmania major. At SBRI, chromosomal sequence is annotated using a combination of trained and untrained non-consensus gene-prediction algorithms with ARTEMIS, an annotation platform with rich and user-friendly interfaces.

Results

Here we describe a methodology used to import results from three different protein-coding gene-prediction algorithms (GLIMMER, TESTCODE and GENESCAN) into the ARTEMIS sequence viewer and annotation tool. Comparison of these methods, along with the CODON USAGE algorithm built into ARTEMIS, shows the importance of combining methods to more accurately annotate the L. major genomic sequence.

Conclusion

An improvised and powerful tool for gene prediction has been developed by importing data from widely-used algorithms into an existing annotation platform. This approach is especially fruitful in the Leishmania genome project where there is large proportion of novel genes requiring manual annotation.

     

Synthesis of a tetra- and a trisaccharide related to an anti-tumor saponin “Julibroside J<Subscript>28</Subscript>” from <Emphasis Type="Italic">Albizia julibrissin</Emphasis>

Simple and convergent synthesis of a tetra- and a trisaccharide portions of an antitumor compound Julibroside J₂₈, isolated from Albizia julibrissin, that showed significant in vitro antitumor activity against HeLa, Bel-7402 and PC-3M-1E8 cancer cell lines is reported. The tetrasaccharide has been synthesized as its p-methoxyphenyl glycoside starting from commercially available d-glucose, l-rhamnose and l-arabinose. The trisaccharide part has been synthesized from commercially available N-acetyl d-glucosamine, d-fucose and d-xylose using simple protecting group manipulations. Sulfuric acid immobilized on silica has been used successfully as a Brönsted acid catalyst for the crucial glycosylation steps.     

Ectopic expression of sorbitol-6-phosphate 2-dehydrogenase gene from <Emphasis Type="Italic">Haloarcula marismortui</Emphasis> enhances salt tolerance in transgenic <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis>

d-Sorbitol-6-phosphate 2-dehydrogenase (S6PDH, E.C. 1.1.1.140) catalyzes the NADH-dependent conversion of d-fructose 6-phosphate (F6P) to d-sorbitol 6-phosphate (S6P). In this work, recombination and characterization of Haloarcula marismortui d-sorbitol-6-phosphate 2-dehydrogenase are reported. Haloarcula marismortui d-sorbitol-6-phosphate 2-dehydrogenase was expressed in P. pastoris and Arabidopsis thaliana. Enzyme assay indicated that HmS6PDH catalyzes the reduction of d-fructose 6-phosphate to d-sorbitol 6-phosphate and HmS6PDH activity was enhanced by NaCl. Furthermore, transgenic A. thaliana ectopic expressing HmS6PDH accumulate more sorbitol under salt stress. These results suggest that the ectopic expression of HmS6PDH in plants can facilitate future studies regarding the engineering and breeding of salt-tolerant crops.     

Some notes onCornaceae

Colmeiroa F. v.Muell. ist a later homonym and therefore a new name is proposed—Paracorokia. Its systematic position is discussed: it seems to belong toCornaceae rather than toEscalloniaceae. SubgenusDiscocrania Harms of the genusCornus is published in a generic rank.     

Die systematische Stellung von Angustiphyllum cuneiforme n. gen. n. sp., einer eigenartigen Tetrakoralle aus dem Mitteldevon Nordspaniens

The newly found tetracoralAngustiphyllum cuneiforme n. gen. n. sp. from theGosseletia-Sandstein (Couvinium) of Candás/Prov. Oviedo (Northern Spain) is described and designed an ancestor of the so called “cuneate corals”Homalophyllum Simpson, 1900,Xenocyathellus Bassler, 1937 andHomalophyllites Easton, 1944 from the Devonian and Mississippian of North-America. Because of their undoubtful genetic relations the whole group of cuneate corals is joined to the subfamily Homalophyllidae n. subfam. within the family ZaphrentoididaeSchindewolf, 1938. Evidently the evolution developed in a progressive orthogenetic manner from the new genesAngustiphyllum (Middle Devonian) toXenocyathellus (higher Middle Devonian) andHomalophyllites (Mississippian), whileHomalophyllum (Middle Devonian) separated from this line in a early stage and thenceforth had its own evolution.     

Flechtensystematische Studien VI. Die GattungSagiolechia Massal.

Eine taxonomische Studie über die GattungSagiolechia Massal. als wieter Teil einer Revision der FamilieGyalectaceae sensuZahlbruckner wird vorgelegt.