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1.
Fauqia Fahmeed Yaima Arocha Rosete Karel Acosta Pérez Eric Boa John Lucas 《Journal of Phytopathology》2009,157(10):639-641
Nearby fruit and vegetable fields in Islamabad, Pakistan were surveyed for phytoplasma infection. ' Candidatus Phytoplasma asteris' (Group 16SrI) was found infecting mango, citrus, loquat, geranium, periwinkle, radish, blackberry and potato. Results suggest that a polyphagous vector may be involved in phytoplasma transmission to these plant species, which are first host records of 16SrI phytoplasma infection in Pakistan. 相似文献
2.
A new TaqMan minor groove binding (MGB) probe and new PCR conditions were designed for quick, specific and sensitive detection of ‘Candidatus Phytoplasma mali’. The new probe can distinguish a single mismatch between ‘Ca. P. mali’ and ‘Candidatus Phytoplasma prunorum’, this constituting an improvement over a previously published method. In our study, the relative position of the mismatch in the MGB probe influenced greatly the specificity of detection. Our new real‐time PCR protocol was able to detect one plasmid copy in water and 100 copies in healthy plant DNA extracts. The sensitivity of this new real‐time PCR method, three other real‐time PCR protocols and a conventional PCR with fU5/rU3 primers was compared. Periwinkles and MM106 rootstocks were grafted with infected material and surveyed over time by symptom observation, conventional PCR and real‐time PCR. Phytoplasma infection was detected by symptom observation in all periwinkles within 4 months and in 75% of the MM106 rootstocks by the end of 7 months. Conventional PCR detected phytoplasma infection in all periwinkles within 4 months and in all MM106 rootstocks within 7 months. Best results were obtained by our real‐time PCR, which detected phytoplasma infection in all grafted plants within 3 months after inoculation. 相似文献
3.
灯叶蝉属Lamprotettix隶属于角顶叶蝉亚科Deltocephalinae 圆冠叶蝉族Athysanini,由Ribaut 以Cicadaoctopunctata Schrank 为模式种建立。Ossiannilsson (1947) 和Ribaut (1952)确认Lamprotettix splendidulus (Fabricius, 1803)和L. octopunctata (Schrank, 1796)是 L.nitidula (Fabricius, 1787) 的异名。该属目前仅知1种 L. nitidula (Fabricius)。本文记述采自我国云南的1新种云南灯叶蝉L. yunnanensis, sp. nov.,至此,该属全世界已知2种,我国均有分布。新种模式标本保存在西北农林科技大学昆虫博物馆。云南灯叶蝉Lamprotettix yunnanensis, 新种(图1~10) 新种与Lamprotettixnitidulus (Fabricius, 1787)相似,但本新种尾节后背缘无三角形突起,阳茎端部仅有1对伸向体前方的突起,不同于后者。 正模♂,云南大理苍山,2400m,1999-Ⅺ-12,秦道正采;副模1♀,同正模。 相似文献
4.
中国角顶叶蝉亚科一新属六新种(同翅目:叶蝉科) 总被引:2,自引:2,他引:2
记述角顶叶蝉亚科1新属:即柔突叶蝉属Abrus gen.nov.,并记述6新种:衡山柔突叶蝉A.hengshanensis sp.nov.,短茎柔突叶蝉A.brevis sp.nov.黄芪柔突叶蝉A.huangi.sp.nov.,武夷柔突叶蝉.A.wuyiensis sp.nov,叉茎柔突叶蝉A.bifurcatus sp.nov。和锥尾柔突叶蝉A.coneus sp.nov.,列有种的检索表,描述了种的外部形态特征及雄性外生殖器特征,附有主要特征图。 相似文献
5.
中国横春叶蝉属一新种记述(同翅目:叶蝉科:角顶叶蝉亚科) 总被引:2,自引:0,他引:2
横春叶蝉属 H engchunia Vilbaste隶属于角顶叶蝉亚科 Deltocephalinae隆脊叶蝉族Paralimini,由 Vilbaste( 1 969)根据分布我国台湾的 H engchunia koshunensis( Matsumu-ra,1 91 4 )为模式种建立 ,当时仅知 1种。Remane & Asche ( 1 980 )建立 1新属 Pseupalus,仅包括模式种 1种 ,Webb & Heller ( 1 990 )又报道该属 3新种。 Asche & Webb ( 1 994)对上述两属进行比较后确认 Pseupalus是横春叶蝉属 H engchunia的异名 ,并发表 4新种。本文记述采自我国海南的 1新种 :短突横春叶蝉 H engchunia breviproducta,sp.nov.,至此 ,该属全世界已知 1 0种 ,我国已知 2种。短突横春叶蝉模式标本保存在西北农林科技大学昆虫博物馆。短突横春叶蝉 Hengchunia breviproducta,新种 (图 1~ 1 0 )本新种与 H engchunia javana Asche & Webb,1 994相似 ,二者阳茎端部均有 1对伸向背侧缘的小突起 ,可与本属其它种相区别 ,但本新种阳茎前腔腹部突起较阳茎干短 ,阳基侧突端部仅有 1个伸向外侧的突起 ,无小的内脊 ,不同于后者。正模 :♂ ,海南两院 ,灯诱 ,1 983 - -0 1 ,张雅林。副模 :1♂ ,海南琼中 ,1 983 - -0 4,张雅林 ;1♀ ,海南通什 ,灯诱 ,1 983 - -0 7,张雅林。 相似文献
6.
叉索叶蝉属NeurotettixMatsumura隶属于角顶叶蝉亚科Deltocephalinae圆冠叶蝉族Athysanini,由Matsumura(1914)根据我国台湾的Neurotettixhorishanus 为模式种建立,当时仅知1种.Ishihara(1963)对该属进行了重新描记并补充了雄性外生殖器特征.蔡平等(1999)报道了河南宝天曼的1个新种--双叉叉索叶蝉Neurotettixbifurcatus.本文记述采自我国湖南的1个新种脊茎叉索叶蝉Neurotettixflangenus, sp. nov.,至此,该属全世界已知3种,全部分布在我国.模式标本保存在西北农林科技大学昆虫博物馆.脊茎叉索叶蝉Neurotettixflangenus, 新种 (图1)新种与Neurotettix horishanus Matsumura相似,但本新种阳茎干较短,端部两侧有侧脊,而后者阳茎干细长,端部向背面弯曲、无突起或脊.本新种也与双叉叉索叶蝉Neurotettixbifurcatus Cai et Shen相似,但本新种端部两侧具脊,后者阳茎端部背缘有1对长突、腹缘有1对短突,明显不同.正模♂,湖南郴州苏仙岭,1985-Ⅶ-18,张雅林、柴勇辉;副模1♀,湖南郴州,1985-Ⅶ-5,张雅林、柴勇辉;1♀,湖南郴州,1985-Ⅶ-16,张雅林、柴勇辉;1♀,湖南郴州,1985-Ⅶ-23,张雅林、柴勇辉. 相似文献
7.
对中国掌叶蝉属利叶蝉亚属进行了分类研究,我国现知4种,即横带掌叶蝉Handianus(Usuirontus)limbicosta (Jacobi)、双斑掌叶蝉H.(U.)limbfer(Matsumura)、冠斑掌叶蝉H.(U.)maculaticeps(Reuter)和条斑掌叶蝉H.(U.)ogikubonis(Matsumura),确认Usuironus quadrimaculatus Cai et Shen,1999是H.(U.)limbifer(Matsumura,1902)的新异名.文中提供了该亚属的鉴别特征和分种检索表、各种的地理分布、形态记述和特征图. 相似文献
8.
Alberto Bressan Jean Larrue & Elisabeth Boudon Padieu 《Entomologia Experimentalis et Applicata》2006,119(1):61-69
The incidence and transmissibility of Flavescence dorée phytoplasma (FDP) in populations of the vector Scaphoideus titanus Ball (Homoptera: Cicadellidae) were investigated by periodically collecting nymphs and adults of the leafhopper species in four vineyards with high incidence of Flavescence dorée (FD)‐diseased grapevines. Insects were tested individually for FDP with an ELISA procedure, after transmission assays to broadbean seedlings and further transmission to grapevine cuttings. No transmission occurred when early or middle instar nymphs were used to inoculate broadbeans, although a limited number of fifth‐instar nymphs and young adults transmitted the pathogen to broadbean seedlings. However, the same batches of insects transmitted FDP more efficiently to grapevine cuttings during prolonged inoculation periods, confirming the existence of a latent period before infected insects become infective. The proportions of ELISA‐positive individuals in the three categories of insects used for transmission assays reflected the rate of FDP transmission to grapevine cuttings. According to the data obtained by ELISA and from field sampling of first‐instar nymphs, we adapted the proportions of nymph hatching, of infected leafhoppers, and of infective leafhoppers (assuming a conservative latent period in the vector of 30 days) to logistic models as a function of degree‐days. We then discussed the possible use of the model developed for improving vector control decisions in FD‐infected vineyards. 相似文献
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10.
Selected behavior of Empoasca fabae (Harris) (Homoptera: Cicadellidae) was examined to elucidate resistance of commercially‐available glandular‐haired alfalfa to this key forage pest. The overall objective was to assess the effects of the glandular trichomes on the behavior of nymphs and adults. Studies of host‐plant acceptance by E. fabae nymphs found first and third instars to show a higher degree of change in settling location on the glandular‐haired FGplh13 alfalfa than on the nonglandular P5373 alfalfa. Nymphs also cleaned their tarsi more frequently when in contact with the glandular trichomes on FGplh13 alfalfa, and in addition a larger number of nymphs jumped/fell off the surface of FGplh13 alfalfa. The glandular trichomes on FGplh13 also impeded nymphal mobility more effectively than the nonglandular trichomes on P5373. No choice, time‐course analysis of adult host‐plant acceptance behavior found that adults settled less frequently on FGplh13 alfalfa with the glandular trichomes intact, than on either FGplh13 with the glandular trichomes removed, or P5373 alfalfa with the nonglandular trichomes intact or removed. Free choice, time‐course analysis of adult host‐preference behavior determined that at each observation, stems of the nonglandular P5373 alfalfa were preferred over FGplh13 alfalfa. Similarly, at each observation, stems of FGplh13 alfalfa with the glandular trichomes removed were preferred over FGplh13 with the glandular trichomes intact. These data provide additional evidence for the localization of a resistance factor in the glandular trichomes of FGplh13 alfalfa. An antixenotic resistance mechanism also appears to be present, which may function, in part, through a tactile avenue. 相似文献
11.
记述角顶叶蝉亚科1新属--背突叶蝉属Protensus,并记述2个新种:周氏背突叶蝉Protensus choui,sp.nov.和茎齿背突叶蝉Protensus dentatus,sp.nov.,建立1个新组合--中国新记录种对柄背突叶蝉Protensus kiushiuensis(Vilbaste),n.comb.,列种的检索表,描述了种的外部形态特征及雄性外生殖器特征,并附主要特征图。模式标本保存在西北农林科技大学昆虫博物馆。 相似文献
12.
Simona Palermo Alessandra Arzone Domenico Bosco 《Entomologia Experimentalis et Applicata》2001,99(3):347-354
Chrysanthemum yellows (CY) phytoplasma is a plant-pathogenic mollicutes belonging to the 16Sr-IB genetic group which infects a variety of dicotyledonous plants and is transmitted in nature by some species of Cicadellidae Deltocephalinae. The transmission characteristics of CY and the factors influencing the vector efficiencies of the leafhoppers Macrosteles quadripunctulatus Kirschbaum and Euscelidius variegatus Kirschbaum are described in the present study using transmission experiments and phytoplasma-specific polymerase chain reaction (PCR) assays. Vector insects were allowed to acquire CY under different experimental conditions and then transferred to healthy test plants for inoculation and/or sampled for DNA extraction and amplification. The transmission efficiency of CY was very high and almost all the leafhoppers became infective following acquisition on CY-infected daisies. The latent period in the vector ranged from 16 to 20 days after the start of the acquisition and infectivity lasted, in general, for life. The PCR assay was successful in detecting CY phytoplasmas in the insects well before they became infective (5 versus 16–18 days) and was used to estimate the proportion of infective insects. When analysed for CY presence by PCR, all the leafhoppers fed for 7–18 days on source daisy reacted positively while, following one day of acquisition, some insects failed to provide amplification. Host-plant species influenced CY acquisition, and daisy appeared a more efficient source for both leafhoppers compared to periwinkle. Life stage did not appear to be critical for CY acquisition, although newly-hatched nymphs of E. variegatus acquired CY less efficiently than fifth instar nymphs. 相似文献
13.
Leafhoppers (Homoptera: Cicadellidae) possess epi-and hypopharyngeal chemosensilla within the head. In this paper, we describe the behaviors of leafhoppers (Graphocephala atropunctata Signoret) which have had two of the four main nerves to these sensilla cut. Insects with severed nerves (treated) and control insects (both sham-operated and normal) were offered a choice of two small mustard leaves infused with either distilled water or a 5% sucrose solution. The control insects showed a preference for the 5% sucrose leaf, whereas the treated leafhoppers did not; they distributed themselves on both leaves with similar frequency. Thus, severing the nerves of only half of the precibarial chemosensilla (epipharyngeal organ) resulted in major changes in leafhopper feeding behavior. This evidence supports the hypothesis that the precibarial chemosensilla mediate gustatory discrimination of chemical compounds within the plant.
Résumé Les cicadelles (Homoptera) possèdent dans la tête des sensilles gustatives sur l'épi et l'hypopharynx. Cette note décrit le comportement de Graphocephala atropunctata Signoret dont deux des quatre principaux nerfs de ces sensilles ont été coupés. Le choix entre des petites feuilles de moutarde infusées, soit dans de l'eau distillée, soit dans une solution de sucrose à 5%, a été proposé à ces insectes et à deux catégories de témoins, les uns intacts, les autres ayant subi une opération à blanc. Les témoins ont montré une préférence pour les feuilles traitées au sucrose, mais pas les cicadelles aux nerfs sectionnés. Les insectes sectionnés ont perforé les deux types de feuilles avec les mêmes fréquences. Ainsi la section de la moitié seulement des nerfs des sensilles gustatives précibariales (organe épipharyngien) a induit un changement fondamental du comportement alimentaire des cicadelles. Cette expérience appuie l'hypothèse selon laquelle les sensilles précibariales fournissent la discrimination gustative des composés chimiques de la plante.相似文献
14.
We have investigated the influence on longevity and fecundity of Flavescence dorée phytoplasma (FDP), the agent of a grapevine yellows disease, in the experimental vector Euscelidius variegatus Kirschbaum. Late instar nymphs were exposed to one or the other of two strains of FDP (FD92 and FD2000) by feeding on infected broad bean (Vicia faba L.) or on healthy broad bean or maize (Zea mays L.) for an acquisition access period of 13 days. Detection of FDP in individual insects was done with PCR assays and revealed that almost all exposed leafhoppers had acquired FDP, for both FD92 and FD2000 strains. FDP infection significantly reduced the life span of males and females (ANOVA of the quartiles of survival distribution and Weibull scale parameter). FDP-exposed females produced significantly fewer nymphs. The two FDP strains had similar effects on reduction of survival and fecundity of leafhoppers. There was no significant differences in longevity of E. variegatus males exposed to FD broad bean than held on healthy broad bean or maize, but female survival and fecundity were reduced when they fed on maize versus healthy broad bean. 相似文献
15.
Alberto Bressan Vincenzo Girolami & Elisabeth Boudon-Padieu 《Entomologia Experimentalis et Applicata》2005,115(2):283-290
Scaphoideus titanus Ball (Homoptera: Cicadellidae), a specialist and univoltine leafhopper on grapevine (Vitis vinifera L.) (Vitaceae), is a vector of Flavescence dorée phytoplasma (FDP) in vineyards of European temperate areas. Males and females of the leafhopper were exposed to FDP by feeding on infected broad bean (Vicia faba L.) (Fabaceae). Detection of FDP by the amplification of phytoplasma DNA with polymerase chain reaction assays of individual insects revealed an acquisition rate of 91.4% (96/105) after an acquisition access period of 13 days. The adult life span of FD‐exposed males and females was much less than that of leafhoppers fed on healthy broad bean, as revealed by ANOVA on the quartiles of survival distribution and Weibull scale parameter. The progeny of exposed females (number of nymphs emerging from eggs deposited on woody cane segments) was significantly less than the progeny of unexposed females. Eggs produced by FD‐exposed females were slightly but significantly delayed in hatching. Reduced fecundity was confirmed by dissecting FD‐exposed and non‐exposed 42‐day‐old females and counting the number of fully sized eggs in each leafhopper. There was no evidence of transovarial passage of FDP in the offspring of infected females after 72 nymphs were reared on a healthy grapevine until the fifth instar or adult appearance and then confined on broad bean seedlings. 相似文献
16.
The leafhopper Amplicephalus curtulus Linnavuori & DeLong (Hemiptera: Cicadellidae) can transmit ‘Candidatus Phytoplasma ulmi’ (16SrV‐A) from a native Chilean shrub, Ugni molinae Turcz. (Myrtaceae), to ryegrasses. A recent study showed that this phytoplasma reduced the total protein content and the activity of detoxifying enzymes in A. curtulus, which could also affect its vector fitness. This study evaluated the effect of ‘Ca. Phytoplasma ulmi’ on the longevity, fecundity, and body mass of A. curtulus. Both females and males were exposed to ‘Ca. Phytoplasma ulmi’‐infected plants for 96 h, whereas a control group remained unexposed. Quartiles from adult emergence to 75% (t75), 50% (t50), and 25% (t25) survival rates were determined for each leafhopper survival distribution. The dry weight was also established at the end of the experiment. The adult lifespan of phytoplasma‐infected males and females was significantly lower than that of the uninfected leafhoppers in quartile survival distributions t50 and t25. The phytoplasma‐infected males and females lived 3 and 4 weeks less than uninfected ones in the last quartile, respectively. Fecundity was established by number of nymphs per female (in four periods) in phytoplasma‐infected and uninfected assays. In general, the weekly pattern of the number of nymphs per phytoplasma‐infected female was lower than that of uninfected leafhoppers; it was 37% lower at the end of the experiment. Phytoplasma‐infected females weighed significantly less (11%) than uninfected individuals. Phytoplasma‐infected males weighed 8% less than uninfected ones, but this difference was not significant. Our data indicated that ‘Ca. Phytoplasma ulmi’ negatively affected the fitness of A. curtulus, and nymphs produced by phytoplasma‐infected females varied over time, which may influence the disease dynamics in nature or in field crops. 相似文献
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18.
Searching and oviposition behavior and parasitization ability of Anagrus nigriventris Girault (Hymenoptera: Mymaridae), an egg parasitoid of beet leafhopper, Circulifer tenellus (Baker) (Homoptera: Cicadellidae), were examined on five host plant species of beet leafhopper: sugar beet (Beta vulgaris L.), red stem filaree (Erodium cicutarium[L.]), peppergrass (Lepidium nitidum Nuttall), desert plantain (Plantago ovata Forsskal), and London rocket (Sisymbrium irio L.). Beet leafhopper embeds its eggs in the tissues of these plant species. For each plant species, A. nigriventris behavior was examined on plants with and without beet leafhopper eggs. Experimental design was a 5 (plant species) by 2 (host eggs present/absent) factorial. Additionally within each treatment, parasitoid behavior was observed over a 22-h period at five different observation periods: t=0, 3, 6, 9, and 22 h where t=0 h represents initial exposure of the insect with the plant. The behavioral events observed were: `fast walking' (general searching), `slow walking' (intensive searching), ovipositor probing, grooming, feeding, and resting. Significant differences (=0.05) among plant species in time spent on the plant, percentage of host eggs parasitized, and behavioral variables associated with intensive searching and oviposition all indicated that the plant species fell into two groups: `preferred' plants (sugar beet, London rocket, and peppergrass), and `unpreferred' plants (filaree and plantago). These variables also indicated that the parasitoids spent more time on, searched more, probed more, and oviposited more in plants with host eggs than plants without host eggs. Consistent effects of time (over the observation periods from t=0 to t=22 h) generally were detected only in the preferred plant species that had host eggs present. In these cases, intensive searching and probing decreased as time advanced, while variables related to general searching (`fast walking') and abandoning host egg patches (leaving the plant) tended to increase over time. 相似文献
19.
Glandular trichomes on perennial alfalfa affect host-selection behavior of Empoasca fabae 总被引:1,自引:0,他引:1
Selected behavior of Empoasca fabae (Harris) (Homoptera: Cicadellidae) was examined to elucidate resistance of commercially-available glandular-haired alfalfa to this key forage pest. The overall objective was to assess the effects of the glandular trichomes on the behavior of nymphs and adults. Studies of host-plant acceptance by E. fabae nymphs found first and third instars to show a higher degree of change in settling location on the glandular-haired FGplh13 alfalfa than on the nonglandular P5373 alfalfa. Nymphs also cleaned their tarsi more frequently when in contact with the glandular trichomes on FGplh13 alfalfa, and in addition a larger number of nymphs jumped/fell off the surface of FGplh13 alfalfa. The glandular trichomes on FGplh13 also impeded nymphal mobility more effectively than the nonglandular trichomes on P5373. No choice, time-course analysis of adult host-plant acceptance behavior found that adults settled less frequently on FGplh13 alfalfa with the glandular trichomes intact, than on either FGplh13 with the glandular trichomes removed, or P5373 alfalfa with the nonglandular trichomes intact or removed. Free choice, time-course analysis of adult host-preference behavior determined that at each observation, stems of the nonglandular P5373 alfalfa were preferred over FGplh13 alfalfa. Similarly, at each observation, stems of FGplh13 alfalfa with the glandular trichomes removed were preferred over FGplh13 with the glandular trichomes intact. These data provide additional evidence for the localization of a resistance factor in the glandular trichomes of FGplh13 alfalfa. An antixenotic resistance mechanism also appears to be present, which may function, in part, through a tactile avenue. 相似文献
20.
R.C. Marucci J.R.S. Lopes J.D. Vendramim & J.E. Corrente 《Entomologia Experimentalis et Applicata》2005,117(2):95-103
Infection of plants by pathogens can influence their attractiveness and suitability to insect vectors and other herbivores. Here we examined the effects of Citrus sinensis (L.) Osbeck (Rutaceae) infection by the bacterium Xylella fastidiosa, which causes citrus variegated chlorosis (CVC), on the feeding preferences of two sharpshooter vectors, Dilobopterus costalimai Young and Oncometopia facialis (Signoret) (Homoptera: Cicadellidae). Experiments were performed inside observation chambers, in which a healthy plant and an infected one (with or without CVC symptoms) were supplied to a group of 40 sharpshooters. The number of insects that selected each treatment was recorded at several time intervals in 48 h. In another experiment, the ingestion rate on healthy and infected (symptomatic or not) plants was evaluated by measuring the liquid excretion of sharpshooters that were confined on branches of each plant for 72 h. Both sharpshooter species preferred healthy plants to those with CVC symptoms. However, O. facialis did not discriminate between healthy citrus and symptomless infected plants. Feeding by D. costalimai was markedly reduced when confined on CVC‐symptomatic plants, but not on asymptomatic infected ones. The ingestion rate by O. facialis was not affected by the presence of CVC symptoms. The results suggest that citrus trees with early (asymptomatic) infections by X. fastidiosa may be more effective as inoculum sources for CVC spread by insect vectors than those with advanced symptoms. 相似文献
