共查询到19条相似文献,搜索用时 265 毫秒
1.
2.
3.
随着近十几年来工业生物技术的发展,有机化合物的生物催化也取得了飞速的进步.近几年的研究集中在:新生物催化剂的筛选和酶的定向改造;非水相生物催化中酶有机溶剂耐受性的增强和非传统介质的应用;生物催化在手性化合物,药物等精细化学品领域的应用;组合生物催化作为组合化学和生物催化相结合而成的一个新技术生长点,并取得一定的进展,为新药的开发提供一种切实可行的方法. 相似文献
4.
生物催化剂是限制工业生物催化的重要瓶颈,发现新型生物催化剂或生物催化剂的新功能及新底物是目前的主要任务。实现该目标的方法有三种:(1)从环境样品中筛选,(2)利用蛋白质工程改造现有生物催化剂,(3)探寻现有生物催化剂的新功能。本文描述了上述三种方法的关键步骤及技术,其中重点介绍了高通量培养技术以及新近发展起来的半理性设计改造生物催化剂的技术。 相似文献
5.
6.
7.
8.
9.
祁国荣 《中国生物工程杂志》1991,11(4):27-32
Ribozyme指一类具有生物催化功能的RNA,也称RNA催化剂、在化学本质上,它不同于具有生物催化功能的蛋白质--酶(enzyme),Ribozyme发现于80年代初,近十年来,ribozyme研究进展深化了生物催化理论,提出了生物大分子和生命起源的新概念。 相似文献
10.
11.
Huihua Sun Hongfang Zhang Ee Lui Ang Huimin Zhao 《Bioorganic & medicinal chemistry》2018,26(7):1275-1284
Biocatalysis has been increasingly used for pharmaceutical synthesis in an effort to make manufacturing processes greener and more sustainable. Biocatalysts that possess excellent activity, specificity, thermostability and solvent-tolerance are highly sought after to meet the requirements of practical applications. Generating biocatalysts with these specific properties can be achieved by either discovery of novel biocatalysts or protein engineering. Meanwhile, chemoenzymatic routes have also been designed and developed for pharmaceutical synthesis on an industrial scale. This review discusses the recent discoveries, engineering, and applications of biocatalysts for the synthesis of pharmaceuticals and pharmaceutical intermediates. Key classes of biocatalysts include reductases, oxidases, hydrolases, lyases, isomerases, and transaminases. 相似文献
12.
Eight papers were presented in this year's symposium "Advances in Biocatalysis" at the 232nd ACS National Meeting, accentuating the most recent development in biocatalysis. Researchers from both industry and academia are addressing several fundamental problems in biocatalysis, including the limited number of commercially available enzymes that can be provided in bulk quantities, the limited enzyme stability and activity in nonaqueous environments, and the permeability issue and cell localization problems in whole-cell systems. A trend that can be discerned from these eight talks is the infusion of new tools and technologies in addressing various challenges facing biocatalysis. Nanotechnology, bioinformatics, cellular membrane engineering and metabolic engineering (for engineering whole-cell catalysts), and protein engineering (to improve enzymes and create novel enzymes) are becoming more routinely used in research laboratories and are providing satisfactory solutions to the problems in biocatalysis. Significant progress in various aspects of biocatalysis from discovery to industrial applications was highlighted in this symposium. 相似文献
13.
《Bioorganic & medicinal chemistry》2014,22(20):5604-5612
Enzymes catalyze a wide range of biotransformations and have a great potential as environmentally friendly alternatives to classical chemical catalysts in various industrial applications. Recently, advanced techniques and strategies in enzyme discovery and engineering have led to the significant expansion of the quantity and functional diversity of biocatalysts, which has further allowed broader uses of biocatalysts in new processes, especially those traditionally enabled only by chemical catalysts. Here we highlight some of these recent advances with the focus on new approaches in biocatalyst discovery and development, and discuss new applications of selected biocatalysts including transaminases, cytochrome P450s, and Baeyer–Villiger monooxygenases. 相似文献
14.
Karina de Godoy Daiha Renata Angeli Sabrina Dias de Oliveira Rodrigo Volcan Almeida 《PloS one》2015,10(6)
The great potential of lipases is known since 1930 when the work of J. B. S. Haldane was published. After eighty-five years of studies and developments, are lipases still important biocatalysts? For answering this question the present work investigated the technological development of four important industrial sectors where lipases are applied: production of detergent formulations; organic synthesis, focusing on kinetic resolution, production of biodiesel, and production of food and feed products. The analysis was made based on research publications and patent applications, working as scientific and technological indicators, respectively. Their evolution, interaction, the major players of each sector and the main subject matters disclosed in patent documents were discussed. Applying the concept of technology life cycle, S-curves were built by plotting cumulative patent data over time to monitor the attractiveness of each technology for investment. The results lead to a conclusion that the use of lipases as biocatalysts is still a relevant topic for the industrial sector, but developments are still needed for lipase biocatalysis to reach its full potential, which are expected to be achieved within the third, and present, wave of biocatalysis. 相似文献
15.
16.
Enzyme catalysis, enabled by advances in protein engineering and directed evolution, is beginning to transform chemical synthesis in the pharmaceutical industry. This review presents recent examples of the creative use of biocatalysis to enable drug discovery and development. We illustrate how increased access to novel biotransformations and the rise of cascade biocatalysis allowed fundamentally new syntheses of novel medicines, representing progress toward more sustainable pharmaceutical manufacturing. Finally, we describe the opportunities and challenges the industry must address to ensure the reduction to practice of biotechnological innovations to develop new therapies in a faster, more economical, and environmentally benign way. 相似文献
17.
生物催化与生物转化研究进展 总被引:1,自引:0,他引:1
由于生物催化过程具有高效、高选择性、条件温和、环境友好等优点,因此成为可持续发展过程中替代和拓展传统有机化学合成的重要方法。近两年的进展集中于新生物催化剂的发现和改造,以及将生物催化和生物转化应用于工业过程的探索,包括开发新的反应体系,新的固定化方法等。可以预见,在医药中间体等高附加值化工产品的生产过程中,生物催化和生物转化的应用将呈现加速增长趋势。 相似文献
18.
Walid Saibi Salma AbdeljalilKhaled Masmoudi Ali Gargouri 《Biochemical and biophysical research communications》2012,426(3):289-293
The chemical industry has come under increasing pressure to make chemical production more eco-friendly and independent to fossil resources. The development of industrial processes based on micro-organisms can especially help to eliminate the use or the generation of hazardous substances and can support the transition from dependence on fossil resources towards real sustainable and eco-safety industrial processes. The biocatalysts are the best solution given by nature that can be used to improve some biotechnological applications. In this research review, we report some peculiar properties of biocatalysts, implicated in a range of metabolic pathways and biotechnological tools. 相似文献
19.
3-脱氢莽草酸,是芳香族氨基酸生物合成代谢途径中一种重要的中间产物,可作为一些化学合成制剂和药物中间原料。这样以无毒可再生物质为起始原料的合成方法与传统的有机合成化学制剂的方法相比,对环境更加有利。此外,它还是一种十分有效的抗氧化剂。工业上一般采用化学合成法和发酵法来生产3-脱氢莽草酸,随着代谢工程的兴起,使得更加理性改造菌株成为可能,这更加促进了发酵法的广泛应用。本文主要介绍了代谢工程在生物合成3-脱氢莽草酸生产菌改造中的应用情况,其中涉及3-脱氢莽草酸生物合成途径中相关基因及其酶的调控、中心代谢途径的改造和3-脱氢莽草酸合成支路的修饰等,并探讨了将来的发展前景。 相似文献