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1.
Stripe or yellow rust of wheat, caused by Puccinia striiformis f. sp. tritici, is an important disease in many wheat-growing regions of the world. A number of major genes providing resistance to stripe rust have been used in breeding, including one gene that is present in the differential tester Carstens V. The objective of this study was to locate and map a stripe rust resistance gene transferred from Carstens V to Avocet S and to use molecular tools to locate a number of genes segregating in the cross Savannah/Senat. One of the genes present in Senat was predicted to be a gene that is present in Carstens V. For this latter purpose, stripe rust response data from both seedling and field tests on a doubled haploid population consisting of 77 lines were compared to an available molecular map for the same lines using a non-parametric quantitative trait loci (QTL) analysis. Results obtained in Denmark suggested that a strong component of resistance with the specificity of Carstens V was located in chromosome arm 2AL, and this was consistent with chromosome location work undertaken in Australia. Since this gene segregated independently of Yr1, the only other stripe rust resistance gene known to be located in this chromosome arm, it was designated Yr32. Further QTLs originating from Senat were located in chromosomes 1BL, 4D, and 7DS and from Savannah on 5B, but it was not possible to characterize them as unique resistance genes in any definitive way. Yr32 was detected in several wheats, including the North American differential tester Tres. 相似文献
2.
Sybil A. Herrera-Foessel Evans S. Lagudah Julio Huerta-Espino Matthew J. Hayden Harbans S. Bariana Davinder Singh Ravi P. Singh 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(1):239-249
The common wheat genotype ‘RL6077’ was believed to carry the gene Lr34/Yr18 that confers slow-rusting adult plant resistance (APR) to leaf rust and stripe rust but located to a different chromosome
through inter-chromosomal reciprocal translocation. However, haplotyping using the cloned Lr34/Yr18 diagnostic marker and the complete sequencing of the gene indicated Lr34/Yr18 is absent in RL6077. We crossed RL6077 with the susceptible parent ‘Avocet’ and developed F3, F4 and F6 populations from photoperiod-insensitive F3 lines that were segregating for resistance to leaf rust and stripe rust. The populations were characterized for leaf rust
resistance at two Mexican sites, Cd. Obregon during the 2008–2009 and 2009–2010 crop seasons, and El Batan during 2009, and
for stripe rust resistance at Toluca, a third Mexican site, during 2009. The F3 population was also evaluated for stripe rust resistance at Cobbitty, Australia, during 2009. Most lines had correlated responses
to leaf rust and stripe rust, indicating that either the same gene, or closely linked genes, confers resistance to both diseases.
Molecular mapping using microsatellites led to the identification of five markers (Xgwm165, Xgwm192, Xcfd71, Xbarc98 and Xcfd23) on chromosome 4DL that are associated with this gene(s), with the closest markers being located at 0.4 cM. In a parallel
study in Canada using a Thatcher × RL6077 F3 population, the same leaf rust resistance gene was designated as Lr67 and mapped to the same chromosomal region. The pleiotropic, or closely linked, gene derived from RL6077 that conferred stripe
rust resistance in this study was designated as Yr46. The slow-rusting gene(s) Lr67/Yr46 can be utilized in combination with other slow-rusting genes to develop high levels of durable APR to leaf rust and stripe
rust in wheat. 相似文献
3.
Peng Zhang Robert A. McIntosh Sami Hoxha Chongmei Dong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,120(1):25-29
Stripe rust is one of the most destructive diseases of wheat. Breeding for resistance is the most economical and environmentally
acceptable means to control stripe rust. Genetic studies on resistance sources are very important. Previous inheritance studies
on Triticum aestivum subsp. spelta cv. album and wheat cultivar Lee showed that each possessed a single dominant gene for stripe rust resistance, i.e., Yr5 and Yr7, respectively. Both were located on the long arm of chromosome 2B, but due to the complexities caused by genetic background
effects there was no clear evidence on the allelism or linkage status of these genes. Our study, involving an intercross of
Avocet S near-isogenic lines possessing the genes, provided clear evidence for allelism or extremely close linkage of Yr5 and Yr7 based on phenotypic and molecular studies. 相似文献
4.
Homoeologous set of NBS-LRR genes located at leaf and stripe rust resistance loci on short arms of chromosome 1 of wheat 总被引:2,自引:0,他引:2
Homoeologous group 1 chromosomes of wheat contain important genes that confer resistance to leaf, stem and stripe rusts,
powdery mildew and Russian wheat aphid. A disease resistance gene analog encoding nucleotide binding site-leucine rich repeat
(NBS-LRR), designated RgaYr10, was previously identified at the stripe rust resistant locus, Yr10, located on chromosome 1BS distal to the storage protein, Gli-B1 locus. RgaYr10 identified gene members in the homoeologous region of chromosome 1DS cosegregating with the leaf rust resistance
gene, Lr21, which originally was transferred from a diploid D genome progenitor. Four RgaYr10 gene members were isolated from chromosome
1DS and compared to two gene members previously isolated from the chromosome 1BS homeologue. NBS-LRR genes tightly linked
to stripe rust resistance gene Yr10 on chromosome 1BS were closely related in sequence and structure to NBS-LRR genes tightly linked to leaf rust resistance
gene Lr21 located within the homoeologous region on chromosome 1DS. The level of sequence homology was similar between NBS-LRR genes
that were isolated from different genomes as compared to genes from the same genome.
Electronic Publication 相似文献
5.
Bansal UK Forrest KL Hayden MJ Miah H Singh D Bariana HS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(8):1461-1466
Two Iranian common wheat landraces AUS28183 and AUS28187 from the Watkins collection showed high levels of seedling resistance
against Australian pathotypes of leaf rust and stripe rust pathogens. Chi-squared analyses of rust response segregation among
F3 populations derived from crosses of AUS28183 and AUS28187 with a susceptible genotype AUS27229 revealed monogenic inheritance
of leaf rust and stripe rust resistance. As both genotypes produced similar leaf rust and stripe rust infection types, they
were assumed to carry the same genes. The genes were temporarily named as LrW1 and YrW1. Molecular mapping placed LrW1 and YrW1 in the short arm of chromosome 5B, about 10 and 15 cM proximal to the SSR marker gwm234, respectively, and the marker cfb309 mapped 8–12 cM proximal to YrW1. LrW1 mapped 3–6 cM distal to YrW1 in two F3 populations. AUS28183 corresponded to the accession V336 of the Watkins collection which was the original source of Lr52. Based on the genomic location and accession records, LrW1 was concluded to be Lr52. Because no other seedling stripe rust resistance gene has previously been mapped in chromosome 5BS, YrW1 was permanently named as Yr47. A combination of flanking markers gwm234 and cfb309 with phenotypic assays could be used to ascertain the presence of Lr52 and Yr47 in segregating populations. This investigation characterised a valuable source of dual leaf rust and stripe rust resistance
for deployment in new wheat cultivars. Transfer of Lr52 and Yr47 into current Australian wheat backgrounds is in progress. 相似文献
6.
Identification and characterization of stripe rust resistance gene Yr34 in common wheat 总被引:5,自引:0,他引:5
Bariana HS Parry N Barclay IR Loughman R McLean RJ Shankar M Wilson RE Willey NJ Francki M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1143-1148
An uncharacterized source of seedling resistance to Puccinia striiformis f.sp. tritici was identified in an advanced wheat breeding line WAWHT2046. Genetic analysis based on a WAWHT2046/Carnamah-derived double
haploid (DH) population demonstrated monogenic inheritance of seedling stripe rust resistance in WAWHT2046. The gene controlling
stripe rust resistance in line WAWHT2046 was tentatively designated YrWA. The chromosome 5AL located awn inhibitor gene B1, possessed by WAWHT2046, also showed monogenic inheritance when the DH population was scored for the presence and absence
of awns. Joint segregation analysis at the B1 and YrWA loci indicated genetic linkage between the two loci. A recombination value of 12.2 cM was computed using Mapmanager. This
association located YrWA in the chromosome arm 5AL. Molecular mapping using microsatellite markers placed YrWA distal to B1. All molecular markers mapped proximal to the awn inhibitor locus B1. As no other stripe rust resistance gene is reported to be located in the chromosome arm 5AL, YrWA was permanently designated as Yr34. Yr34 produced an intermediate (23C) seedling infection type and expressed very low stripe rust response (10R-MR) on adult plants
in the field, similar to the resistance gene Yr17. In addition to Yr34, this mapping population segregated for three genetically independent adult plant stripe rust resistance genes. The detection
of DH lines with completely susceptible response, higher than that shown by the Yr34-lacking parent Carnamah, suggested that both parents contributed adult plant resistance. The use of WAWHT2046 as a parent
in breeding programs would also contribute APR in addition to Yr34. 相似文献
7.
NBS-LRR sequence family is associated with leaf and stripe rust resistance on the end of homoeologous chromosome group 1S of wheat 总被引:7,自引:2,他引:5
W. Spielmeyer L. Huang H. Bariana A. Laroche B. S. Gill E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1139-1144
A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii and wheat. At least two unrelated resistance-gene analogs (RGAs) mapped close to known leaf rust resistance genes (Lr21 and Lr40) located distal to seed storage protein genes on chromosome 1DS. One of the two RGA clones, which was previously shown to
be part of a candidate gene for stripe rust resistance (Yr10) located within the homoeologous region on 1BS, identified at least three gene family members on chromosome 1DS of Ae. tauschii. One of the gene members co-segregated with the leaf rust resistance genes, Lr21 and Lr40, in Ae. tauschii and wheat segregating families. Hence, a RGA clone derived from a candidate gene for stripe rust resistance located on chromosome
1BS detected candidate genes for leaf rust resistance located in the corresponding region on 1DS of wheat.
Received: 10 January 2000 / Accepted: 25 March 2000 相似文献
8.
W. E. Wen G. Q. Li Z. H. He W. Y. Yang M. L. Xu X. C. Xia 《Molecular breeding : new strategies in plant improvement》2008,22(4):507-515
The gene Yr26 confers resistance to all races of Puccinia striiformis f. sp. tritici (PST), the casual pathogen of wheat stripe rust in China. Here, we report development of a molecular marker closely linked
to Yr26 using a resistance gene-analog polymorphism (RGAP) technique. A total of 787 F2 plants and 165 F3 lines derived from the cross Chuanmai 42/Taichung 29 were used for linkage analysis. Eighteen near-isogenic lines (NILs)
and 18 Chinese wheat cultivars and advanced lines with different genes for stripe rust resistance were employed for the validation
of STS markers. A total of 1,711 RGAP primer combinations were used to test the parents and resistant and susceptible bulks.
Five polymorphic RGAP markers were used for genotyping all F2 plants. Linkage analysis showed that the five RGAP markers were closely linked to Yr26 with genetic distances ranging from 0.5 to 2.9 cM. These markers were then converted into STS markers, one, CYS-5, of which was located 0.5 cM to Yr26 and was closely associated with the resistance gene when validated over 18 NILs and 18 Chinese wheat cultivars and lines.
The results indicated that CYS-5 can be used in marker-assisted selection targeted at pyramiding Yr26 and other genes for stripe rust resistance. 相似文献
9.
Spielmeyer W Singh RP McFadden H Wellings CR Huerta-Espino J Kong X Appels R Lagudah ES 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(4):481-490
The Lr34/Yr18 locus has contributed to durable, non-race specific resistance against leaf rust (Puccinia triticina) and stripe rust (P. striiformis f. sp. tritici) in wheat (Triticum aestivum). Lr34/Yr18 also cosegregates with resistance to powdery mildew (Pm38) and a leaf tip necrosis phenotype (Ltn1). Using a high resolution mapping family from a cross between near-isogenic lines in the “Thatcher” background we demonstrated
that Lr34/Yr18 also cosegregated with stem rust resistance in the field. Lr34/Yr18 probably interacts with unlinked genes to provide enhanced stem rust resistance in “Thatcher”. In view of the relatively
low levels of DNA polymorphism reported in the Lr34/Yr18 region, gamma irradiation of the single chromosome substitution line, Lalbahadur(Parula7D) that carries Lr34/Yr18 was used to generate several mutant lines. Characterisation of the mutants revealed a range of highly informative genotypes,
which included variable size deletions and an overlapping set of interstitial deletions. The mutants enabled a large number
of wheat EST derived markers to be mapped and define a relatively small physical region on chromosome 7DS that carried Lr34/Yr18. Fine scale genetic mapping confirmed the physical mapping and identified a genetic interval of less than 0.5 cM, which contained
Lr34/Yr18. Both rice and Brachypodium genome sequences provided useful information for fine mapping of ESTs in wheat. Gene order was more conserved between wheat
and Brachypodium than with rice but these smaller grass genomes did not reveal sequence information that could be used to identify a candidate
gene for rust resistance in wheat. We predict that Lr34/Yr18 is located within a large insertion in wheat not found at syntenic positions in Brachypodium and rice.
W. Spielmeyer and R. P. Singh contributed equally to the study through the “Thatcher” and “Lalbahadur” genetic stocks, respectively. 相似文献
10.
Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A 总被引:10,自引:0,他引:10
Inheritance studies showed that the VPM1-derived seedling resistances to stem rust, stripe rust, leaf rust, and powdery mildew were controlled by single genes; the genes for rust resistance were designated Sr38, Yr17, and Lr37, respectively, whereas the gene for resistance to powdery mildew was postulated to be Pm4b. Sr38, Yr17, and Lr37 were shown to be closely linked and distally located in the short arm of chromosome 2A. They showed very close repulsion linkage with Lr17 and were genetically independent of other genes known to be located in chromosome 2A. Previously unmapped, Yr1 appeared to be distally located in the long arm of chromosome 2A. 相似文献
11.
Uauy C Brevis JC Chen X Khan I Jackson L Chicaiza O Distelfeld A Fahima T Dubcovsky J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,112(1):97-105
Several new races of the stripe rust pathogen have become frequent throughout the wheat growing regions of the United States since 2000. These new races are virulent to most of the wheat seedling resistance genes limiting the resistance sources that can be used to combat this pathogen. High-temperature adult-plant (HTAP) stripe rust resistance has proven to be more durable than seedling resistance due to its non-race-specific nature, but its use is limited by the lack of mapping information. We report here the identification of a new HTAP resistance gene from Triticum turgidum ssp. dicoccoides (DIC) designated as Yr36. Lines carrying this gene were susceptible to almost all the stripe rust pathogen races tested at the seedling stage but showed adult-plant resistance to the prevalent races in California when tested at high diurnal temperatures. Isogenic lines for this gene were developed by six backcross generations. Field tests in two locations showed increased levels of field resistance to stripe rust and increased yields in isogenic lines carrying the Yr36 gene compared to those without the gene. Recombinant substitution lines of chromosome 6B from DIC in the isogenic background of durum cv. Langdon were used to map the Yr36 gene on the short arm of chromosome 6B completely linked to Xbarc101, and within a 2-cM interval defined by PCR-based markers Xucw71 and Xbarc136. Flanking locus Xucw71 is also closely linked to the grain protein content locus Gpc-B1 (0.3-cM). Marker-assisted selection strategies are presented to improve stripe rust resistance and simultaneously select for high or low Gpc-B1 alleles. 相似文献
12.
Spielmeyer W McIntosh RA Kolmer J Lagudah ES 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):731-735
The incorporation of effective and durable disease resistance is an important breeding objective for wheat improvement. The leaf rust resistance gene Lr34 and stripe rust resistance gene Yr18 are effective at the adult plant stage and have provided moderate levels of durable resistance to leaf rust caused by Puccinia triticina Eriks. and to stripe rust caused by Puccinia striiformis Westend. f. sp. tritici. These genes have not been separated by recombination and map to chromosome 7DS in wheat. In a population of 110 F7 lines derived from a Thatcher × Thatcher isogenic line with Lr34/Yr18, field resistance to leaf rust conferred by Lr34 and to stripe rust resistance conferred by Yr18 cosegregated with adult plant resistance to powdery mildew caused by Blumeria graminis (DC) EO Speer f. sp. tritici. Lr34 and Yr18 were previously shown to be associated with enhanced stem rust resistance and tolerance to barley yellow dwarf virus infection. This chromosomal region in wheat has now been linked with resistance to five different pathogens. The Lr34/Yr18 phenotypes and associated powdery mildew resistance were mapped to a single locus flanked by microsatellite loci Xgwm1220 and Xgwm295 on chromosome 7DS. 相似文献
13.
Kuraparthy V Chhuneja P Dhaliwal HS Kaur S Bowden RL Gill BS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1379-1389
Leaf rust and stripe rust are important foliar diseases of wheat worldwide. Leaf rust and stripe rust resistant introgression
lines were developed by induced homoeologous chromosome pairing between wheat chromosome 5D and 5Mg of Aegilops geniculata (UgMg). Characterization of rust resistant BC2F5 and BC3F6 homozygous progenies using genomic in situ hybridization with Aegilops comosa (M) DNA as probe identified three different types of introgressions; two cytologically visible and one invisible (termed
cryptic alien introgression). All three types of introgression lines showed similar and complete resistance to the most prevalent
pathotypes of leaf rust and stripe rust in Kansas (USA) and Punjab (India). Diagnostic polymorphisms between the alien segment
and recipient parent were identified using physically mapped RFLP probes. Molecular mapping revealed that cryptic alien introgression
conferring resistance to leaf rust and stripe rust comprised less than 5% of the 5DS arm and was designated T5DL·5DS-5MgS(0.95). Genetic mapping with an F2 population of Wichita × T5DL·5DS-5MgS(0.95) demonstrated the monogenic and dominant inheritance of resistance to both diseases. Two diagnostic RFLP markers, previously
mapped on chromosome arm 5DS, co-segregated with the rust resistance in the F2 population. The unique map location of the resistant introgression on chromosome T5DL·5DS-5MgS(0.95) suggested that the leaf rust and stripe rust resistance genes were new and were designated Lr57 and Yr40. This is the first documentation of a successful transfer and characterization of cryptic alien introgression from Ae. geniculata conferring resistance to both leaf rust and stripe rust in wheat. 相似文献
14.
Molecular Detection of Stripe Rust Resistance Gene(s) in 115 Wheat Cultivars (lines) from the Yellow and Huai River Valley Wheat Region 下载免费PDF全文
Qian Li Baotong Wang Kaixiang Chao Juan Guo Jianrong Song Weiyun Yue Qiang Li 《Journal of Phytopathology》2016,164(11-12):946-958
Stripe rust (yellow rust), caused by Puccinia striiformis f.sp. tritici (Pst), is a serious disease of wheat worldwide, including China. Growing resistant cultivars is the most cost‐effective and environmentally friendly approach to control the disease. To assess the stripe rust resistance in commercial wheat cultivars and advanced lines in the Yellow and Huai River Valley Wheat Region, 115 wheat cultivars (lines) collected from 13 provinces in this region were evaluated with the most prevalent Chinese Pst races CYR32, CYR33 and the new race V26 at seedling stage. In addition, these wheat entries were inoculated with the mixed races of CYR32 and CYR33 at the adult‐plant stage in the field. The results indicated that 53 (46.1%) cultivars (lines) had all‐stage resistance to all the three races, and 16 (13.9%) cultivars (lines) showed adult‐plant resistance. The possible stripe rust resistance genes in these entries were postulated by the closely linked markers of all‐stage resistance genes Yr5, Yr9, Yr10, Yr15 and Yr26 and adult‐plant resistance gene Yr18. Molecular analysis indicated that resistance genes Yr5, Yr9, Yr10, Yr18 and Yr26 were found in 5 (4.3%), 38 (33.0%), 1 (0.9%), 2 (1.7%) and 8 (7.0%) entries, respectively. No entry was found to carry the Yr15 gene. In future breeding programs, Yr5, Yr15 and Yr18 should be used to pyramid with other effective genes to develop wheat cultivars with high‐level and durable resistance to stripe rust, whereas Yr9, Yr10 and Yr26 should not be used or used in a limited way due to the virulent races present in China. 相似文献
15.
Rosewarne GM Singh RP Huerta-Espino J Herrera-Foessel SA Forrest KL Hayden MJ Rebetzke GJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(7):1283-1294
Leaf rust and stripe rust are important diseases of wheat world-wide and deployment of cultivars with genetic resistance is
an effective and environmentally sound control method. The use of minor, additive genes conferring adult plant resistance
(APR) has been shown to provide resistance that is durable. The wheat cultivar ‘Pastor’ originated from the CIMMYT breeding
program that focuses on minor gene-based APR to both diseases by selecting and advancing generations alternately under leaf
rust and stripe rust pressures. As a consequence, Pastor has good resistance to both rusts and was used as the resistant parent
to develop a mapping population by crossing with the susceptible ‘Avocet’. All 148 F5 recombinant inbred lines were evaluated under artificially inoculated epidemic environments for leaf rust (3 environments)
and stripe rust (4 environments, 2 of which represent two evaluation dates in final year due to the late build-up of a new
race virulent to Yr31) in Mexico. Map construction and QTL analysis were completed with 223 polymorphic markers on 84 randomly selected lines in
the population. Pastor contributed Yr31, a moderately effective race-specific gene for stripe rust resistance, which was overcome during this study, and this was
clearly shown in the statistical analysis. Linked or pleiotropic chromosomal regions contributing to resistance against both
pathogens included Lr46/Yr29 on 1BL, the Yr31 region on 2BS, and additional minor genes on 5A, 6B and 7BL. Other minor genes for leaf rust resistance were located on 1B,
2A and 2D and for stripe rust on 1AL, 1B, 3A, 3B, 4D, 6A, 7AS and 7AL. The 1AL, 1BS and 7AL QTLs are in regions that were
not identified previously as having QTLs for stripe rust resistance. The development of uniform and severe epidemics facilitated
excellent phenotyping, and when combined with multi-environment analysis, resulted in the relatively large number of QTLs
identified in this study. 相似文献
16.
Dadkhodaie NA Karaoglou H Wellings CR Park RF 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):479-487
The rust resistance genes Lr53 and Yr35, transferred to common wheat from Triticum dicoccoides, were reported previously to be completely linked on chromosome 6B. Four F
3 families were produced from a cross between a line carrying Lr53 and Yr35 (98M71) and the leaf rust and stripe rust susceptible genotype Avocet “S” and were rust tested using Puccinina triticina pathotype 53-1,(6),(7),10,11 and Puccinia striiformis f. sp. tritici pathotype 110 E143 A+. The homozygous resistant lines produced infection types of “;1−” and “;N” to these pathotypes, respectively.
The Chi-squared tests indicated goodness-of-fit of the data for one leaf rust gene and one stripe rust gene segregation. Linkage
analysis using this population demonstrated recombination of 3% between the genes. Microsatellite markers located on the short
arm of chromosome 6B were used to map the genes, with the markers cfd1 and gwm508 being mapped approximately 1.1 and 4.5 cM, respectively, proximal to Lr53. Additional studies of the relationship between Lr36, also located on the short arm of chromosome 6B, and Lr53 indicated that the two genes were independent. 相似文献
17.
Wheat production in Pakistan is seriously constrained due to rust diseases and stripe rust (yellow) caused by Puccinia striiformis f. sp. tritici, which could limit yields. Thus development and cultivation of genetically diverse and resistant varieties is the most sustainable
solution to overcome these diseases. The first objective of the present study was to evaluate 100 Pakistan wheat cultivars
that have been grown over the past 60 years. These cultivars were inoculated at the seedling stage with two virulent stripe
rust isolates from the United States and two from Pakistan. None of the wheat cultivars were resistant to all tested stripe
rust isolates, and 16% of cultivars were susceptible to the four isolates at the seedling stage. The data indicated that none
of the Pakistan wheat cultivars contained either Yr5 or Yr15 genes that were considered to be effective against most P. striiformis f. sp. tritici isolates from around the world. Several Pakistan wheat cultivars may have gene Yr10, which is effective against isolate PST-127 but ineffective against PST-116. It is also possible that these cultivars may
have other previously unidentified genes or gene combinations. The second objective was to evaluate the 100 Pakistan wheat
cultivars for stripe rust resistance during natural epidemics in Pakistan and Washington State, USA. It was found that a higher
frequency of resistance was present under field conditions compared with greenhouse conditions. Thirty genotypes (30% of germplasms)
were found to have a potentially high temperature adult plant (HTAP) resistance. The third objective was to determine the
genetic diversity in Pakistan wheat germplasms using molecular markers. This study was based on DNA fingerprinting using resistance
gene analog polymorphism (RGAP) marker analysis. The highest polymorphism detected with RGAP primer pairs was 40%, 50% and
57% with a mean polymorphism of 36%. A total of 22 RGAP markers were obtained in this study. RGAP, simple sequence repeat
(SSR) and sequence tagged site (STS) markers were used to determine the presence and absence of some important stripe rust
resistance genes, such as Yr5, Yr8, Yr9, Yr15 and Yr18. Of the 60 cultivars analyzed, 17% of cultivars showed a RGAP marker band for Yr9 and 12% of cultivars exhibited the Yr18 marker band. No marker band was detected for Yr5, Yr8 and Yr15, indicating a likely absence of these genes in the tested Pakistan wheat cultivars. Cluster analysis based on molecular and
stripe rust reaction data is useful in identifying considerable genetic diversity among Pakistan wheat cultivars. The resistant
germplasms identified with 22 RGAP markers and from the resistance evaluations should be useful in developing new wheat cultivars
with stripe rust resistance. 相似文献
18.
Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34 总被引:1,自引:0,他引:1
X. L. Zhou D. J. Han X. M. Chen H. L. Gou S. J. Guo L. Rong Q. L. Wang L. L. Huang Z. S. Kang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(11):2349-2358
Key message
We report a new stripe rust resistance gene on chromosome 7AS in wheat and molecular markers useful for transferring it to other wheat genotypes.Abstract
Several new races of the stripe rust pathogen have established throughout the wheat growing regions of China in recent years. These new races are virulent to most of the designated seedling resistance genes limiting the resistance sources. It is necessary to identify new genes for diversification and for pyramiding different resistance genes in order to achieve more durable resistance. We report here the identification of a new resistance gene, designated as Yr61, in Chinese wheat cultivar Pindong 34. A mapping population of 208 F2 plants and 128 derived F2:3 lines in a cross between Mingxian 169 and Pindong 34 was evaluated for seedling stripe rust response. A genetic map consisting of eight resistance gene analog polymorphism (RGAP), two sequence-tagged site (STS) and four simple sequence repeat (SSR) markers was constructed. Yr61 was located on the short arm of chromosome 7A and flanked by RGAP markers Xwgp5467 and Xwgp5765 about 1.9 and 3.9 cM in distance, which were successfully converted into STS markers STS5467 and STS5765b, respectively. The flanking STS markers could be used for marker-assisted selection of Yr61 in breeding programs. 相似文献19.
Australian cultivar Sunco carries three adult plant stripe rust resistance genes. One of these genes corresponded to Yr18 in chromosome 7DS; the second, YrCK, was mapped on chromosome 2D. Here, we describe the characterization of the third adult plant resistance (APR) gene from Sunco. Sunco/2*Avocet S-derived lines SA65 (resistant) and SA67 (susceptible) were crossed and a recombinant inbred line F6 population was generated. Monogenic segregation among SA65/SA67-derived RIL population was demonstrated and the resistance locus was designated YrSA3. Selective genotyping using an iSelect 90 K Infinium SNP array and SSR markers located YrSA3 on chromosome 3D. Development of KASP markers for SNP loci showing association with YrSA3 allowed construction of a genetic map harboring the resistance gene. Ten KASP markers (KASP_8306, KASP_9142, KASP_10438, KASP_16434, KASP_17207, KASP_20836, KASP_23518, KASP_23615, KASP_57983 and KASP_63653), one SSR marker (gwm114b) and Lr24/Sr24 were mapped 1.8 cM distal to YrSA3. Comparison of marker data indicated that the previously named seedling stripe rust resistance gene Yr45 was located proximal to YrSA3, and therefore the latter was formally designated Yr71. Two recombinants carrying Lr24/Sr24 and Yr71 in combination were identified for use as donor sources in wheat breeding programs. The robustness of gwm114b, KASP_16434, KASP_17207 and KASP_20836 for marker-assisted selection of these genes was demonstrated through tests on 74 Australian wheat cultivars. 相似文献
20.
Remapping of the stripe rust resistance gene <Emphasis Type="Italic">Yr10</Emphasis> in common wheat
Cuiling Yuan Jingzheng Wu Baiqiang Yan Qunqun Hao Chaozhong Zhang Bo Lyu Fei Ni Allan Caplan Jiajie Wu Daolin Fu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1253-1262