共查询到20条相似文献,搜索用时 343 毫秒
1.
Ashley MV Wilk JA Styan SM Craft KJ Jones KL Feldheim KA Lewers KS Ashman TL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1201-1207
The objectives of the present study were to develop microsatellite markers for the wild strawberry, Fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. A genomic library was screened for microsatellite repeats and several PCR primers were designed and tested. We also tested the use of heterologous primers and found that F. virginiana primers amplified products in cultivated strawberry, Fragaria × ananassa Duch. and Fragaria chiloensis. Similarly, microsatellite loci developed from cultivated strawberry also successfully amplified F. virginiana loci. We investigated four microsatellite loci in detail, three developed from F. virginiana and one from cultivated strawberry. A survey of 100 individuals from a population of F. virginiana in Pennsylvania demonstrated high heterozygosities (He or gene diversity ranged from 0.80 to 0.88 per locus) and allelic diversity (12–17 alleles per locus), but individual plants had no more than two alleles per locus. Segregation patterns in parents and progeny of two controlled crosses at these four loci were consistent with disomic Mendelian inheritance. Together these findings suggest that the genome of F. virginiana is "highly diploidized" and at least a subset of microsatellite loci can be treated as codominant, diploid markers. Significant heterozygote deficiencies were found at three of the four loci for hermaphroditic individuals but for only one locus among females in this gynodioecious species.Communicated by J. Dvorak 相似文献
2.
RAFAEL M. ALVES ALEXANDRE M. SEBBENN ANGELA S. ARTERO ANTONIO FIGUEIRA 《Molecular ecology resources》2006,6(4):1219-1221
Theobroma grandiflorum (cupuassu) is an important fruit tree native to the Brazilian Amazon. Forty‐eight microsatellite loci developed for the congener Theobroma cacao were tested in cupuassu, and 29 (60.4%) produced robust alleles. The analyses of 216 cupuassu accessions using the 21 polymorphic microsatellite loci revealed a total of 113 alleles. The number of alleles per polymorphic locus ranged from two to 11, with an average of 5.38 alleles per locus. The average observed heterozygosity was 0.343, while the mean expected heterozygosity was 0.614. The successful transferability of T. cacao microsatellite primers to cupuassu was consistent with currently accepted phylogeny. 相似文献
3.
Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for
this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the
number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564.
These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic
variations in broodstocks of freshwater prawn, M. rosenbergii. 相似文献
4.
T. Yamamoto K. Mochida T. Imai Y. Z. Shi I. Ogiwara T. Hayashi 《Molecular ecology resources》2002,2(3):298-301
Twenty‐four and 12 microsatellite loci were developed in peach [Prunus persica (L) Batsch cv. Akatsuki] by using an enriched genomic and fruit cDNA libraries, respectively. The microsatellite loci obtained from an enriched library produced 1–9 alleles per locus, 24 in total, of which 22 showed polymorphisms. The average values of observed and expected heterozygosities among the 24 loci were 0.15 and 0.68, respectively. The microsatellite loci derived from cDNA showed 1–7 alleles per locus. Eight sequences showed significant homology to the registered genes in a database. 相似文献
5.
J. A. Galarza S. Roques J. Carreras-Carbonell E. Macpherson G. F. Turner C. Rico 《Conservation Genetics》2007,8(5):1251-1253
Eight polymorphic microsatellite loci were isolated and characterized for the Cardinal fish (Apogon imberbis), a coastal-reef fish endemic to the Mediterranean Sea. Characterization of 30 Cardinal fish individuals form the western
Mediterranean showed moderate to high allelic diversity ranging from 6 to 19 alleles per locus. Two loci showed significant
departures from Hardy-Weinberg equilibrium presumably due to null alleles. No evidence of linkage disequilibrium was found
for any locus pairwise comparasions. This microsatellite set could be useful for any basic population genetic studies of this
species. 相似文献
6.
MADELEINE J. H. VAN OPPEN JIM N. UNDERWOOD ANDY N. MUIRHEAD LESA PEPLOW 《Molecular ecology resources》2007,7(3):436-438
Here we present nine novel, polymorphic microsatellite loci developed for the scleractinian coral Acropora millepora (Acroporidae) from the Great Barrier Reef. In addition, we have assessed the specificity and polymorphism of five microsatellite loci previously developed for a Caribbean congener and one locus developed for an Indo‐Pacific congener. Only one of the latter six loci produced reliable results, yielding a total of 10 polymorphic microsatellite loci for A. millepora. Variability was tested on 20–23 individuals from a single population, plus another ~10 individuals from each of three different populations, resulting in five to 20 alleles per locus. 相似文献
7.
José Gabriel Segarra-Moragues Gabriela Gleiser Fernando González-Candelas 《Conservation Genetics》2008,9(4):1059-1062
An enrichment protocol was used to isolate and characterize microsatellite loci in Acer opalus, a Mediterranean tree species. Highly polymorphic microsatellite loci were required for paternity analyses in a population
of this species. Eight microsatellite loci were amplified and a total of 87 alleles were detected in a sample of 142 individuals
from one population, allowing the identification of each individual with a unique multilocus genotype. The paternity exclusion
probabilities varied from 0.261 for locus Aop820 to 0.806 for locus Aop450, and the parent-pair exclusion probabilities varied
from 0.433 for Aop820 to 0.940 for Aop450. The cumulative probabilities of exclusion for paternity and parentage of the eight
loci were both higher than 0.999, supporting the usefulness of these microsatellite loci for future paternity and parentage
analyses in A. opalus. Cross-species transferability was also assayed, supporting their potential use in other eight Acer species. 相似文献
8.
Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass.
Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea
bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations
of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to
0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic
markers developed for striped bass.
Received November 9, 1999; accepted February 11, 2000. 相似文献
9.
Juan F. Fern’andez Victoria L. Sork Gerardo Gallego Jershon L’opez Adriana Bohorques Joe Tohme 《Plant Molecular Biology Reporter》2000,18(4):397-397
Conservation of 15 out of 24 previously identified microsatellite loci (62.5%), was found in a survey of the South American
oak,Quercus humboldtii. The number of alleles per locus varied from 2 to 20, detecting at least 5 microsatellite loci with 5 or more alleles. This
number of loci and alleles is adequate for most studies of genetic diversity and gene flow analysis. In addition, a method
for extracting DNA from mature oak leaves is described that minimizes oxidation of tannins, a common problem in silica-gel-dried
samples. The microsatellite markers detected in this study and the DNA extraction protocol may be applied to more than 30
species ofQuercus that exist in tropical America. 相似文献
10.
Seven-band grouper (Epinephelus septemfasciatus) is a commercially important fishery species. Sixty-six microsatellite loci were isolated from a dinucleotide-enriched genomic
library of E. septemfasciatus. Twelve of these loci were polymorphic in a test population with alleles per locus ranging from two to five, and observed
and expected heterozygosities per locus from 0.04 to 1.00 and from 0.28 to 0.76, respectively. Three loci significantly deviated
from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci after Bonferroni
correction. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of E. septemfasciatus and other related species.
Lili Zhao and Changwei Shao have contributed equally. 相似文献
11.
Song-Lin Chen Lili Zhao Chang-Wei Shao Xiaolin Liao Yong-Sheng Tian 《Conservation Genetics》2009,10(4):1101-1103
Black sea bass (Centropristis striata) is an economically important serranid species. A number of 39 microsatellite loci were isolated from two enriched genomic
library of C. striata. Eleven of these loci were polymorphic in a test population with alleles per locus ranging from 3 to 8, and observed and
expected heterozygosities per locus from 0.26 to 1.00 and from 0.61 to 0.84, respectively. Four loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of C. striata and other related species. 相似文献
12.
Jing-Feng Yang Li-Li Zhao Chang-Wei Shao Xiang-Shan Ji Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(5):1541-1543
Black snapper Sebastodes fuscescens (Houttuyn) is an economocally important species. Fourty-five microsatellite loci were isolated from an enriched genomic library
of S. fuscescens. Ten of these loci were polymorphic in a test population with alleles per locus ranging from 2 to 5, and observed and expected
heterozygosities per locus from 0.21 to 1.00 and from 0.19 to 0.86, respectively. Three loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic
microsatellite loci would be useful for investigating genetic diversity of S. fuscescens and other related species. 相似文献
13.
C. E. EDWARDS M. ARAKAKI P. F. QUINTANA‐ASCENCIO D. E. SOLTIS P. S. SOLTIS 《Molecular ecology resources》2007,7(6):1135-1137
We report the isolation of 19 primer pairs for amplification of polymorphic microsatellite loci for Hypericum cumulicola. These markers were evaluated in 24 individuals from one population; two to four alleles were detected per locus, and observed heterozygosity ranged from 0 to 0.5. Two loci demonstrated significant heterozygote deficiencies, possibly due to null alleles, and significant linkage disequilibrium was found between six pairs of loci. The remaining microsatellite loci will help determine if genetic differentiation is responsible for life‐history differences between natural and anthropogenically disturbed populations of H. cumulicola. 相似文献
14.
Isolation and characterization of polymorphic microsatellite loci from fat greenling (Hexagrammos otakii) 总被引:2,自引:0,他引:2
Song-Lin Chen Gui-Dong Miao Chang-Wei Shao Yong-Sheng Tian Xiaolin Liao 《Conservation Genetics》2009,10(5):1429-1431
Fat greenling (Hexagrammos otakii) is an economocally important marine fish species. Thirtyfive microsatellite loci were isolated from two enriched genomic
library of Hexagrammos otakii. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.2581 to 1.0000 and from 0.2892 to 0.7726, respectively. Four loci significantly
deviated from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between
pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Hexagrammos otakii. 相似文献
15.
ALFREDO AMIEL P. LEONARDÍA PRAKASH P. KUMAR BENITO C. TAN 《Molecular ecology resources》2006,6(2):396-398
We isolated and characterized microsatellite loci for the palaeotropic moss, Acanthorrhynchium papillatum. Eight loci tested on 98 gametophytic samples generated four to 26 alleles per locus with genetic diversities ranging from 0.578 to 0.936. These microsatellite loci are now being used as genetic markers for studies on the effects of deforestation on moss populations in South‐East Asia. 相似文献
16.
Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Hong-Yu Ma Yong-Sheng Tian Song-Lin Chen 《Conservation Genetics》2009,10(3):701-703
Barfin flounder (Verasper moseri) is a rare fish species in the world. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of barfin flounder (Verasper moseri). The number of alleles, observed, and expected heterozygosity per locus in a test population ranged from 2 to 6, from 0.3333
to 1.0000, and from 0.4866 to 0.7774, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification
of these microsatellite loci in additional five fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in Verasper moseri.
Gui-Dong Miao and Chang-Wei Shao Contributed equally to this work. 相似文献
17.
Song-Lin Chen Shi-Chao Xing Gen-Bo Xu Xiaolin Liao Jing-Feng Yang 《Conservation Genetics》2009,10(5):1469-1471
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library
of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis. 相似文献
18.
K. M. Miller K. Laberee K. H. Kaukinen S. Li R. E. Withler 《Molecular ecology resources》2001,1(4):315-317
Twelve novel di‐, tri‐ and tetranucleotide microsatellite loci to the pinto abalone (Haliotis kamtschatkana) are described. Over 400 individuals were analysed at each microsatellite locus. Observed heterozygosities ranged from 0.44 to 0.93, and numbers of alleles from 20 to 63. Six of the loci contained excesses in homozygosity indicative of inbreeding, nonrandom mating, population admixture, or null alleles. 相似文献
19.
JOHN H. MATTHEWS SANDRA BOLES CAMILLE PARMESAN THOMAS JUENGER 《Molecular ecology resources》2007,7(5):845-847
Fourteen polymorphic microsatellite loci were developed from an enriched genomic library of the widely distributed migratory North American dragonfly species, the common green darner (Anax junius). For a group of 22 larvae, these loci averaged 16 alleles, with individual loci ranging from nine to 29 alleles. Observed heterozygosity averaged 0.784 per locus. 相似文献
20.
Katsura M. Kawano 《Molecular ecology resources》2003,3(2):314-315
Six microsatellite loci were developed for a passerine bird, the great tit (Parus major), using two methods. These loci were polymorphic (3–8 alleles per locus) and exhibited expected heterozygosities from 0.45 to 0.77. At one locus the genotypic frequencies deviated significantly from Hardy–Weinberg expectations. 相似文献