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Tongkun Liu Xiaoming Song Weike Duan Zhinan Huang Gaofeng Liu Ying Li Xilin Hou 《Plant Molecular Biology Reporter》2014,32(5):1041-1056
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Global Expression Profiling of Transcription Factor Genes Provides New Insights into Pathogenicity and Stress Responses in the Rice Blast Fungus 总被引:1,自引:0,他引:1
Sook-Young Park Jaeyoung Choi Se-Eun Lim Gir-Won Lee Jongsun Park Yang Kim Sunghyung Kong Se Ryun Kim Hee-Sool Rho Junhyun Jeon Myung-Hwan Chi Soonok Kim Chang Hyun Khang Seogchan Kang Yong-Hwan Lee 《PLoS pathogens》2013,9(6)
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《Saudi Journal of Biological Sciences》2023,30(6):103652
The challenge of climate change makes it mandatory to improve tolerance to drought stress in bread wheat (Triticum aestivum) via biotechnological approaches. Drought stress experiment was conducted followed by RNA-Seq analysis for leaves of two wheat cultivars namely Giza 168 and Gemmiza 10 with contrasting genotypes. Expression patterns of the regulated stress-related genes and concordantly expressed TFs were detected, then, validated via qPCR for two loss-of-function mutants in Arabidopsis background harboring mutated genes analogue to those in wheat. Drought-stress related genes were searched for concordantly expressed TFs and a total of eight TFs were shown to coexpress with 14 stress-related genes. Among these genes, one TF belongs to the zinc finger protein CONSTANS family and proved via qPCR to drive expression of a gene encoding a speculative TF namely zinc transporter 3-like and two other stress related genes encoding tryptophan synthase alpha chain and asparagine synthetase. Known functions of the two TFs under drought stress complement those of the two concordantly expressed stress-related genes, thus, it is likely that they are related. This study highlights the possibility to utilize metabolic engineering approaches to decipher and incorporate existing regulatory frameworks under drought stress in future breeding programs of bread wheat. 相似文献
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