The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
A type of compact and cost‐effective light‐sheet imaging device, termed sub‐voxel‐resolving light‐sheet add‐on module (SLAM), is developed to cooperate with conventional 2D epifluorescence microscope, allowing high‐contrast, resolution‐improved 3D imaging of various biological samples at high throughput. Further details can be found in the article by Fang Zhao, Yicong Yang, Yi Li, et al. ( e201960243 ).
The figure shows the detailed morphology of vasculature and dynamic changes of the blood vessel diameter and density and the oxygen saturation in the blood vessels in fetal brain after acute prenatal ethanol exposure in the second‐trimester equivalent murine model obtained using a real‐time photoacoustic tomography (PAT) system. Further details can be found in the article by Tianqi Shan, Yuan Zhao, Shixie Jiang, Huabei Jiang ( e201960161 ).
A new high‐speed cellular imaging method using clinically applicable moxifloxacin labeling, called as moxifloxacin based confocal microscopy (MBCM), was developed for fast and sensitive tumor detection and delineation. The MBCM demarcated malignant brain tumor from normal brain by visualizing dense and irregular cell distribution in the tumor. An image processing algorithm was developed for automated brain tumor detection. Further details can be found in the article by Seunghun Lee, Won Yeong Park, Hoonchul Chang, et al. ( e201900197 ).
Transcranial photobiomodulation (tPBM) with wavelength at 808 nm was applied to a rat model of acute seizures. The tPBM successfully attenuated convulsive status epilepticus, and the tPBM was shown to reduce the apoptosis of parvalbumin‐positive interneurons (PV‐INs) and preserved the integrity of perisomatic inhibitory network of PV‐INs to principle cells in the hippocampus. Further details can be found in the article by Chung‐Min Tsai, Shwu‐Fen Chang, and Hsi Chang ( e202000095 ).
This study presents a novel intraoperative in vivo imaging approach which harnessed Cerenkov luminescence (CL) to detect primary and metastatic colorectal cancer (CRC) using clinically approved radiopharmaceuticals. In the mice and swine experiments, the proposed approach effectively improved the effect of CRC surgery. The approach is believed to be promising for utilizing CL in open surgery. Further details can be found in the article by Zeyu Zhang, Yawei Qu, Yu Cao et al. ( e201960152 )
A dual‐raster‐scanned photoacoustic microscope (DRS‐PAM) was reported, which integrates a two‐dimensional motorized translation stage for large field‐of‐view imaging and a two‐axis fast galvanometer scanner for real‐time imaging. The DRS‐PAM provides a flexible transition from wide‐field monitoring the vasculature of organs to real‐time imaging of local dynamics. Further details can be found in the article by Fei Yang, Zhiyang Wang, Wuyu Zhang, et al. ( e202000022 ).
A false‐colored and merged image of fresh, ex vivo rat kidney acquired using an excitation‐scanning hyperspectral imaging system. The spectral image was acquired using excitation wavelengths from 360 to 550 nm. Colors represent principal components extracted from a spectral image cube featuring no added labels or markers. Further details can be found in the article by Peter F. Favreau, Joshua A. Deal, Bradley Harris, et al. ( e201900183 ).
This study proposed a Sparse‐Graph Manifold Learning (SGML) method to balance the sparseness and morphology preserving for bioluminescence tomography reconstruction. It inherits the benefits of non‐convex sparsity constraint and dynamic Laplacian graph model. The results of numerical simulations and in vivo experiments demonstrate that the proposed method yields accurate and robust results in terms of tumor spatial location and morphology recovery. Further details can be found in the article by Hongbo Guo, Ling Gao, Jingjing Yu, et al. ( e201960218 )
The family of vibrational spectroscopic imaging techniques grows every few years and there is a need to compare and contrast new modalities with the better understood ones. Three vibrational spectroscopy techniques (High Definition FT‐IR, Raman and AFM‐IR) were applied for subcellular chemical imaging of cholesteryl esters in PC‐3 prostate cancer cells. The techniques were compared and contrasted in terms of image quality, spectral pattern, and chemical information. Further details can be found in the article by Maciej Roman, Tomasz P. Wrobel, Czeslawa Paluszkiewicz, and Wojciech M. Kwiatek ( e201960094 ).
A high‐efficiency computer‐aided diagnostic model of ovarian cancer was developed, integrating SHG imaging technology for non‐invasive imaging of living tissue and machine learning method based on radiomics and TPOT. This model can rapidly, non‐destructively, and accurately perform ovarian cancer diagnosis and has great potential in improving diagnostic efficacy and efficiency of medical pathologists. Further details can be found in the article by Guangxing Wang, Yang Sun, Youting Chen, Qiqi Gao, Dongqing Peng, Hongxin Lin, Zhenlin Zhan, Zhiyi Liu, and Shuangmu Zhuo ( e202000050 ).
iSERS (SERS=surface‐enhanced Raman scattering) microscopy is an emerging Raman‐based staining technique for the selective localization of target proteins on cells and tissues using antibody‐ SERS nanotag conjugates. In this contribution we demonstrate the feasibility of iSERS for imaging of programmed cell death‐ligand 1 (PD‐L1), an important predictive biomarker, on single SkBr‐3 breast cancer cells. Further details can be found in the article by Elzbieta Stepula, Matthias König, Xin‐Ping Wang, et al. ( e201960034 ).
A 3D printed stereotaxic head mount that enables high resolution imaging of the anterior chamber of the eye in alert and freely mobile mice is designed in this study. The system is placed non‐invasively using temporal mount bars and a snout mount, without breaking the skin or risking suffocation, while an endoscopic channel stabilizes the ocular probes. Further details can be found in the article by Bjorn Paulson, Sangwook Lee, Miyeon Jue, Kyungsung Lee, Sanghwa Lee, Guk Bae Kim, Youngjin Moon, Joo Yong Lee, Namkug Kim, and Jun Ki Kim ( e201960188 ).
Confocal Raman microscopy provides composition and constitution of label-free samples at high spatial-resolution. In this study, the histology of atherosclerotic arteries was measured using a custom-built confocal Raman microscopy that was developed to improve imaging speed, diffraction efficiency, and spectral resolution. Additionally, the machine learning method was used to enhance the accuracy of classification. Results show that each layer of arteries and lipid-rich inflamed plaque can be successfully characterized. Further details can be found in the article by Jingchao Xing, Dong-Ryoung Lee, Jin Won Kim, and Hongki Yoo ( e202200243 ).
Continuous-wave terahertz reflection imaging is a potential tool for biological tissues. Based on our home-made continuouswave terahertz reflection imaging system, the effect of both polarization mode and reflection window on the imaging performance is studied theoretically and experimentally, showing good agreement. By taking obtaining sample information and image contrast into consideration, p-polarized terahertz waves are recommended. Moreover, considering the sample boundary identification and the image contrast, selection criteria for reflection window are proposed. This work will help to improve the performance of continuous-wave terahertz reflection imaging and accelerate the THz imaging in biological application. Further details can be found in the article by Limin Wu, Yuye Wang, Haibin Li, Zelong Wang, Meilan Ge, Degang Xu, and Jianquan Yao ( e202100245 ).
Infrared spectroscopy enables insight into light‐activated riboflavin‐induced collagen cross‐linking for future intervertebral disc treatment and repair. Further details can be found in the article by Ioannis Vasilikos, Julian Haas, Graciosa Q. Teixeira, Julia Nothelfer, Cornelia Neidlinger‐Wilke, Hans‐Joachim Wilke, Andreas Seitz, Demetrios G. Vavvas, Josef Zentner, Jürgen Beck, Ulrich Hubbe, and Boris Mizaikoff ( e202000110 ).
A novel 0.9 mm-diameter intravascular photoacoustic catheter with coaxial excitation and detection was developed to overcome the limitation of imaging range. A miniature ring-shaped ultrasound transducer with a 0.18 mm-diameter orifice in the center was successfully fabricated. The results demonstrated that the coaxial catheter exhibited much better photoacoustic/ultrasound imaging performance from the intima to the adventitia. Further details can be found in the article by Riqiang Lin, Qi Zhang, Shengmiao Lv, Jiaming Zhang, Xiatian Wang, Dongliang Shi, Xiaojing Gong, and Kwok-ho La ( e202200269 ).
A novel volumetric information fusion based on joint weighted evaluation and stationary wavelet transform is proposed. Threedimensional stationary wavelet transform was performed on multi-focus data to obtain wavelet coefficients. Differential evolution based on joint weighted evaluation was then employed to optimize the block size of division. Corresponding sub-coefficients of multi-focus data were fused with the proposed fusion rule. Finally, large volumetric and high-resolution photoacoustic imaging can be achieved by applying the inverse stationary wavelet transform. Further details can be found in the article by Xianlin Song, Sihang Li, Zhuangzhuang Wang, and| Xiongjun Cao ( e202200234 )
A comparative analysis of intrinsic skin aging between Caucasian and Asian subjects by slide‐free in vivo harmonic generation microscopy is reported. The cellular and nuclear areas of basal cells in Caucasians and Asians are found to increase at the same rate, ideal for scoring age. On the other hand, the viable epidermis thickness, the dermal papilla volume and height in Caucasians are found to decrease faster than those in Asians. Further details can be found in the article by Kuan‐Hung Lin, Yi‐Hua Liao, Ming‐Liang Wei, and Chi‐Kuang Sun ( e201960063 ).
