Das exoskelet von Notiothauma reedi MacLachlan,ein beitrag zur morphologie und phylogenie der Mecoptera (Insecta)

The present contribution deals with the exoskeleton of Notiothauma reedi MacLachlan. A special attention has been paid to the external male genital apparatus with particular reference to the sperm-pump. Finally the structures, which have a bearing on the phylogeny of the order Mecoptera have been duly discussed.

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Abkürzungen a Analis - apx Höcker am Analkomplex - at vordere Tentorialgrube - Atf antennifer - au Auxilium - Ax Axillare - Ba Basalare - BaSp Basalarspalt - bbsch mediales Borstenbüschel an der Lacinien-Basis - bc Basicostalsklerit - bcb Basicostalborsten - bsk Basalsklerit der Maxille - bt Basituberculus des Gonostylus - c Costa - Cd Cardo - Cer Cercus - Clp Clypeus - cu Cubitus - Cx Coxa - DCv Dorsocervicale - Dej Ductus ejaculatorius - ec vorderes Mandibelgelenk - Epm Epimerum - Eps Episternum - es Epistomalsutur - fb Frenularborsten - fl laterobasaler Membranflügel des Labrum - Fl Flagellum - flg Flügel des Pumpenkörpersklerites - For Foramen occipitale - Fr Frons - fs Fortsatz der Subgena - fspmk Fortsätze des Pumpenkörpersklerites - Ga Galea - gb Gonobasis - Ge Gena - gel Gelenk zwischen Pumpenkörpersklerit und Pistillträger - gesp nicht verschlossener Teil der Genitalspalte - glfl Gleitfläche des Pumpenkörpersklerites - GP Gelenkfortsatz - gst Gonostylus - h Humeralader - ha Hypandrium - hc hinteres Mandibelgelenk - hdr mediale Hakendornen der Lacinia - hsk Halshautsklerit - HP Humeralplatte - jb Jugumborsten - Ju Jugum - l Leiste auf dem Clypeolabrum - la lappiger Fortsatz am Hinterende der Genitalfalten - lblc laterale Borstenreihe der Lacinia - lblr lateraler Borstensaum des labrum - Lc Lacinia - LCv Laterocervicale - lepi lateraler Fortsatz des Epiandrium - lm mediale Lamelle des Pumpenkörpersklerites - loc Lateralocellus - Lr Labrum - lsplc laterale Spange der Lacinia - lth laterales Tergalhorn des T_VI und T_VII - m Media - Md Mandibel - mepi medianer Fortsatz des Epiandrium - Mer Meron - mld Mulde des Pumpenkörpersklerites - mOc Medianocellus - MP Mittelplatte - mt Mediotuberculus des Gonostylus - mth medianes Tergalhorn des T_V - N Notum - Ng Nygma - no Notalorgan - ns Sulcus auf dem Pronotum - Occ Occiput - ocs Occipitalnaht - pcs Praecoxalsutur - Pd Pedicellus - pf pfannenartiger Anhang des Pumpenkörpersklerites - Pge Postgena - PgeL Postgenallobus - Plb Palpus labialis - plFlG pleurales Flügelgelenk - plHG pleurales Hüftgelenk - PlS Pleuralsutur - pmk Pumpenkörpersklerit - Pmt Postmentum - Pmx Palpus maxillaris - PN Postnotum - po Postnotalorgan - PrEps Praeepisternum - Prm Praementum - PrSct Praescutum - ps Pistill - PSge Processus subgenalis - Pst Pterostigma - pstr Pistillträger - pt hintere Tentorialgrube - qua terminale Borstenquaste - qua terminale Borstenquaste der Maxille - r Radius - rbga rückwärtige Borstenreihe der Galea - rs Sector radii - rsplc rückwärtige Spange der Lacinia - sa Furcalgrube - Sa Subalare - sc Subcosta - Sc Scapus - Scl Scutellum - Sct Scutum - Sge Subgena - sgs Subgenalnaht - sl terminaler Seitenlappen des Labrum - SL sternaler Gelenkfortsatz für die Coxa - spga laterale Spange der Galea - sprn Spermarinne des Pumpenkörpersklerites - St Sternum - Stg Stigma - STg Subtegula - Sti Stipes - sto Stylarorgan - T Tergum - TB Tentorialbrücke - TG Tegula - Thy Thyridium - Tn Trochantinus - Tr Trochanter - trs Transversalsutur des N₁ - ts Sulcus temporalis - u membranbekleidete Medialseite des Pumpenkörpersklerites - ub Unguitractorborsten - Un Unguis - Utr Unguitractor - Vx Vertex - w wulstartige Verdickung am Hinterende des Flügels des Pumpenkörpersklerites - wnkgr Winkelgruben - wnkz Winkelzähne - x Anhang am Hinterende der linken Genitalfalte - y caudales Ende der Genitalspalte - z l. Aufgabelung der Media - zi zipfelförmiger Anhang des Flügels des Pumpenkörpersklerites     

T cell requirement for development of chronic ulcerative dermatitis in E- and P-selectin-deficient mice

C57BL/6 mice deficient in E- and P-selectin (E(-/-)P(-/-)) kept under specific pathogen-free barrier conditions have high circulating neutrophil counts and develop hypercellular cervical lymph nodes with substantial plasma cell infiltrates, severe ulcerative dermatitis, conjunctivitis, and lung pathology, which eventually lead to premature death. To test the hypothesis that the pathology in E(-/-)P(-/-) mice may be caused by dysfunctional lymphocyte activity, we crossed E(-/-)P(-/-) mice with recombination activation gene (Rag)-1(-/-) mice to generate E(-/-)P(-/-)Rag-1(-/-) mice lacking mature T and B lymphocytes. E(-/-)P(-/-)Rag-1(-/-) mice had circulating neutrophil counts and plasma G-CSF levels similar to E(-/-)P(-/-) mice. Remarkably, none of the E(-/-)P(-/-)Rag-1(-/-) mice developed conjunctivitis or ulcerative dermatitis typical of E(-/-)P(-/-) mice. These mice were overall healthier in appearance than E(-/-)P(-/-) mice, and histopathologic changes in the lung were reduced. Cervical lymph nodes in E(-/-)P(-/-)Rag-1(-/-) mice were much smaller than those of E(-/-)P(-/-) mice, containing few mononuclear cells and no plasma cells. These data show that the severe disease phenotype of E(-/-)P(-/-) mice depends on lymphocyte function. We conclude that a dysregulated immune response in E(-/-)P(-/-) mice causes disease development, but is not necessary for elevated neutrophil counts.     

The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit.

The amino acid sequences of both the alpha and beta subunits of human chorionic gonadotropin have been determined. The amino acid sequence of the alpha subunit is: Ala - Asp - Val - Gln - Asp - Cys - Pro - Glu - Cys-10 - Thr - Leu - Gln - Asp - Pro - Phe - Ser - Gln-20 - Pro - Gly - Ala - Pro - Ile - Leu - Gln - Cys - Met - Gly-30 - Cys - Cys - Phe - Ser - Arg - Ala - Tyr - Pro - Thr - Pro-40 - Leu - Arg - Ser - Lys - Lys - Thr - Met - Leu - Val - Gln-50 - Lys - Asn - Val - Thr - Ser - Glu - Ser - Thr - Cys - Cys-60 - Val - Ala - Lys - Ser - Thr - Asn - Arg - Val - Thr - Val-70 - Met - Gly - Gly - Phe - Lys - Val - Glu - Asn - His - Thr-80 - Ala - Cys - His - Cys - Ser - Thr - Cys - Tyr - Tyr - His-90 - Lys - Ser. Oligosaccharide side chains are attached at residues 52 and 78. In the preparations studied approximately 10 and 30% of the chains lack the initial 2 and 3 NH2-terminal residues, respectively. This sequence is almost identical with that of human luteinizing hormone (Sairam, M. R., Papkoff, H., and Li, C. H. (1972) Biochem. Biophys. Res. Commun. 48, 530-537). The amino acid sequence of the beta subunit is: Ser - Lys - Glu - Pro - Leu - Arg - Pro - Arg - Cys - Arg-10 - Pro - Ile - Asn - Ala - Thr - Leu - Ala - Val - Glu - Lys-20 - Glu - Gly - Cys - Pro - Val - Cys - Ile - Thr - Val - Asn-30 - Thr - Thr - Ile - Cys - Ala - Gly - Tyr - Cys - Pro - Thr-40 - Met - Thr - Arg - Val - Leu - Gln - Gly - Val - Leu - Pro-50 - Ala - Leu - Pro - Gin - Val - Val - Cys - Asn - Tyr - Arg-60 - Asp - Val - Arg - Phe - Glu - Ser - Ile - Arg - Leu - Pro-70 - Gly - Cys - Pro - Arg - Gly - Val - Asn - Pro - Val - Val-80 - Ser - Tyr - Ala - Val - Ala - Leu - Ser - Cys - Gln - Cys-90 - Ala - Leu - Cys - Arg - Arg - Ser - Thr - Thr - Asp - Cys-100 - Gly - Gly - Pro - Lys - Asp - His - Pro - Leu - Thr - Cys-110 - Asp - Asp - Pro - Arg - Phe - Gln - Asp - Ser - Ser - Ser - Ser - Lys - Ala - Pro - Pro - Pro - Ser - Leu - Pro - Ser-130 - Pro - Ser - Arg - Leu - Pro - Gly - Pro - Ser - Asp - Thr-140 - Pro - Ile - Leu - Pro - Gln. Oligosaccharide side chains are found at residues 13, 30, 121, 127, 132, and 138. The proteolytic enzyme, thrombin, which appears to cleave a limited number of arginyl bonds, proved helpful in the determination of the beta sequence.     

Distribution of NT-IR perikarya in the brain of the guinea pig with special reference to cardiovascular centers in the medulla oblongata

Summary The occurrence and distribution of neurotensin-immunoreactive (NT-IR) perikarya was studied in the central nervous system of the guinea pig using a newly raised antibody (KN 1). Numerous NT-IR perikarya were found in the nuclei amygdaloidei, nuclei septi interventriculare, hypothalamus, nucleus parafascicularis thalami, substantia grisea centralis mesencephali, ventral medulla oblongata, nucleus solitarius and spinal cord. The distribution of NT-IR perikarya was similar to that previously described in the rat and monkey. In the gyrus cinguli, hippocampus and nucleus olfactorius, though, no NT-IR neurons were detected in this investigation. Additional immunoreactive perikarya, however, were observed in areas of the ventral medulla oblongata, namely in the nucleus paragigantocellularis, nucleus retrofacialis and nucleus raphe obscurus.The relevance of the NT-IR perikarya within the ventral medulla oblongata is discussed with respect to other neuropeptides, which are found in this area, and to cardiovascular regulation.Abbreviations abl nucleus amygdaloideus basalis lateralis - abm nucleus amygdaloideus basalis medialis - acc nucleus amygdaloideus centralis - aco nucleus amygdaloideus corticalis - ahp area posterior hypothalami - ala nucleus amygdaloideus lateralis anterior - alp nucleus amygdaloideus lateralis posterior - ame nucleus amygdaloideus medialis - atv area tegmentalis ventralis - bst nucleus proprius striae terminalis - CA commissura anterior - CC corpus callosum - cgld corpus geniculatum laterale dorsale - cglv corpus geniculatum laterale ventrale - cgm corpus geniculatum mediale - CHO chiasma opticum - CI capsula interna - co nucleus commissuralis - cod nucleus cochlearis dorsalis - cp nucleus caudatus/Putamen - cs colliculus superior - cu nucleus cuneatus - dmh nucleus dorsomedialis hypothalami - DP decussatio pyramidum - em eminentia mediana - ent cortex entorhinalis - epi epiphysis - FLM fasciculus longitudinalis medialis - fm nucleus paraventricularis hypothalami pars filiformis - FX fornix - gd gyrus dentatus - gp globus pallidus - gr nucleus gracilis - hl nucleus habenulae lateralis - hm nucleus habenulae medialis - hpe hippocampus - ift nucleus infratrigeminalis - io oliva inferior - ip nucleus interpeduncularis - LM lemniscus medialis - MT tractus mamillo-thalamicus - na nucleus arcuatus - nls nucleus lateralis septi - nms nucleus medialis septi - npca nucleus proprius commissurae anterioris - ns nucleus solitarius - n III nucleus nervi oculomotorii - nt V nucleus tractus spinalis nervi trigemini - ntm nucleus mesencephalicus nervi trigemini - osc organum subcommissurale - P tractus cortico-spinalis - PC pedunculus cerebri - PCI pedunculus cerebellaris inferior - pir cortex piriformis - pol area praeoptica lateralis - pom area praeoptica medialis - prt area praetectalis - pt nucleus parataenialis - pvh nucleus paraventricularis hypothalami - pvt nucleus paraventricularis thalami - r nucleus ruber - re nucleus reuniens - rgi nucleus reticularis gigantocellularis - rl nucleus reticularis lateralis - rm nucleus raphe magnus - ro nucleus raphe obscurus - rp nucleus raphe pallidus - rpc nucleus reticularis parvocellularis - rpgc nucleus reticularis paragigantocellularis - sch nucleus suprachiasmaticus - SM stria medullaris thalami - snc substantia nigra compacta - snl substantia nigra lateralis - snr substantia nigra reticularis - ST stria terminalis - tad nucleus anterior dorsalis thalami - tam nucleus anterior medialis thalami - tav nucleus anterior ventralis thalami - tbl nucleus tuberolateralis - tc nucleus centralis thalami - tl nucleus lateralis thalami - tmd nucleus medialis dorsalis thalami - TO tractus opticus - TOL tractus olfactorium lateralis - tpo nucleus posterior thalami - tr nucleus reticularis thalami - trs nucleus triangularis septi - TS tractus solitarius - TS V tractus spinalis nervi trigemini - tvl nucleus ventrolateralis thalami - vmh nucleus ventromedialis hypothalami - vh ventral horn, Columna anterior - zi zona incerta Supported by the Deutsche Forschungsgesellschaft (DFG) SFB 90, Carvas     

Die Entwicklung vonLanice conchilega (Pallas) mit besonderer Berücksichtigung der Lebensweise

Ohne ZusammenfassungAbkürzungen a Auge - af After - al Alge - ap Analpapille - ba Basalplatte - bl Blastomeren - bu Buccalorgan - da Darmanlage - de Detritus - do Dotter - dorw dosaler Wimperring - dorwp dorsale Wimpern - dph Diaphragma - ed Enddarm - ep Episphäre - flz flüssigkeitsgefüllte Zellen - gr Grubenorgan - h Haarborste - hak Haken - hakflo Hakentragende Flossen - hakwl hakentragende Wülste - har starre Härchen - hy Hyposphäre - km Kiemen - lb Larvalborsten - ll Laterallappen - lt Lateraltentakel - lw laterale Wülste - ma Magen - mak Makromeren - md Mund - me Mediantentakel - medwl mediane Wülste - metneph Metanephridien - mik Mikromeren - oe Oesophagus - oel Öltröpfchen - ol Oberlippe - ph Pharynx - pi Pigment - plfs Plasmaflasche - por Porus - pr Prostomium - ptr Prototroch - ptrneph Protonephridien - py Pygidium - s Scheitelplatte - schl Schleim - sg Segment - st Statocyste - strem strukturierte Eimembran - swp Scheitelwimpern - t Tentakel - ta Tentakelanlage - te Telotroch - tr Trochoblast - ul Unterlippe - umz Urmesodermzelle - wl Wulst (Mit 12 Abbildungen im Text, 6 Tafeln und 1 Tabelle)Inaugural-Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Universität Hamburg.     

Protection of atherogenesis in thromboxane A2 receptor-deficient mice is not associated with thromboxane A2 receptor in bone marrow-derived cells

In the previous study, we generated mice lacking thromboxane A2 receptor (TP) and apolipoprotein E, apoE(-/-)TP(-/-) mice, and reported that the double knockout mice developed markedly smaller atherosclerotic lesions than those in apoE(-/-) mice. To investigate the mechanism responsible for reduced atherosclerosis in apoE(-/-)TP(-/-) mice, we examined the role of TP in bone marrow (BM)-derived cells in the development of the atherosclerotic lesions. When we compared the function of macrophages in apoE(-/-) and in apoE(-/-)TP(-/-) mouse in vitro, there was no difference in the expression levels of cytokines and chemokines after stimulation with lipopolysaccharide. We then transplanted the BM from either apoE(-/-) or apoE(-/-)TP(-/-) mice to either apoE(-/-) or apoE(-/-)TP(-/-) mice after sublethal irradiation. After 12 weeks with high fat diet, we analyzed the atherosclerotic lesion of aortic sinus. When the BM from apoE(-/-) or apoE(-/-)TP(-/-) mice was transplanted to apoE(-/-) mice, the lesion size was almost the same as that of apoE(-/-) mice without BM transplantation. In contrast, when the BM from apoE(-/-) or apoE(-/-)TP(-/-) mice was transplanted to apoE(-/-)TP(-/-) mice, the lesion size was markedly reduced. These results indicate that the protection of atherogenesis in TP(-/-) mice is not associated with TP in BM-derived cells.     

Synthesis of enantiopure 2-aryl-2-methoxypropionic acids and determination of their absolute configurations by X-ray crystallography

Racemic 2-aryl-2-methoxypropionic acids were enantioresolved by the use of (S)-(-)-phenylalaninol 4. For instance, racemic 2-methoxy-2-phenylpropionic acid (+/-)-7 was condensed with phenylalaninol (S)-(-)-4 yielding a diastereomeric mixture of amides, which was easily separated by HPLC on silica gel affording the first-eluted amide (-)-13a and the second-eluted amide (+)-13b: alpha = 3.19, Rs = 3.49. The absolute configuration of amide (-)-13a was determined to be (R;S) by X-ray crystallography by reference to the S configuration of the phenylalaninol moiety. Amide (R;S)-(-)-13a was converted to oxazoline (R;S)-(-)-14a, from which enantiopure 2-methoxy-2-phenylpropionic acid (R)-(-)-7 was recovered. Other 2-aryl-2-methoxypropionic acids, (R)-(-)-8, (R)-(-)-9, (R)-(+)-10, (R)-(-)-11, and (R)-(-)-12, were similarly prepared in enantiopure forms with the use of phenylalaninol (S)-(-)-4, and their absolute configurations were clearly determined by X-ray crystallography or by chemical correlation.     

Isolation and characterization of linear polylactosamines containing one and two site-specifically positioned Lewis x determinants: WGA agarose chromatography in fractionation of mixtures generated by random, partial enzymatic alpha3-fucosylation of pure polylactosamines

We report that isomeric monofucosylhexasaccharides, Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1- 3Galbeta1-4(Fucalpha1-3) GlcNAc, Galbeta1-4GlcNAcbeta1-3Galbeta1-4(Fucalpha1-3) GlcNAcbeta1-3Galbeta1-4 GlcNAc and Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1- 4GlcNAcbeta1-3Galbeta1-4 GlcNAc, and bifucosylhexasaccharides Galbeta1-4GlcNAcbeta1-3Galbeta1-4(Fucalpha1-3) GlcNAcbeta1-3Galbeta1-4(Fucalpha1-3)GlcNAc, Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1- 4GlcNAcbeta1-3Galbeta1-4 (Fucalpha1-3)GlcNAc and Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4( Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4GlcNAc can be isolated in pure form from reaction mixtures of the linear hexasaccharide Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1- 3Galbeta1-4GlcNAc with GDP-fucose and alpha1,3-fucosyltransferases of human milk. The pure isomers were characterized in several ways;1H-NMR spectroscopy, for instance, revealed distinct resonances associated with the Lewis x group [Galbeta1-4(Fucalpha1-3)GlcNAc] located at the proximal, middle, and distal positions of the polylactosamine chain. Chromatography on immobilized wheat germ agglutinin was crucial in the separation process used; the isomers carrying the fucose at the reducing end GlcNAc possessed particularly low affinities for the lectin. Isomeric monofucosyl derivatives of the pentasaccharides GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1- 4Gl cNAc and Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4G lcN Ac and the tetrasaccharide Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAc were also obtained in pure form, implying that the methods used are widely applicable. The isomeric Lewis x glycans proved to be recognized in highly variable binding modes by polylactosamine-metabolizing enzymes, e.g., the midchain beta1,6-GlcNAc transferase (Lepp?nen et al., Biochemistry, 36, 13729-13735, 1997).     

Sand dwelling Turbellaria from the Netherlands Delta area

Sand dwelling Turbellaria from the Delta of the Rivers Rhine, Meuse and Scheldt have been investigated. Thirty-eight samples taken from littoral and sublittoral stations in the Grevelingen, Eastern and Western Scheldt have been analysed.Thirty-three species were recorded (Acoela were not considered); twenty-four of them are new for the area and seven new species are described.Density and diversity of Turbellaria were higher in the Eastern Scheldt than in the Western Scheldt or in the Lake Grevelingen. A maximum density of 82 ind./100 cm³ was noted. A tentative calculation on relative abundance of the representatives of the different Turbellaria orders is established. Proseriata seem to be dominant in the localities studied.Abbreviations acg : accessory glands - aco : accessory organ - ad : atrial diverticle - b : bursa - br : brain - cil : cilia - cm : circular muscle - cn : cnidosac - co : copulatory organ - cs : cuticular spines - css : cuticular stylet sheat - de : ejaculatory duct - di : ductus intervesicularis - ds : seminal duct - dsp : spermatic duct - en : enteron - fd : female duct - fp : femal pore - ga : genital atrium - gf : glands in female duct - gg : glands - gp : genital pore - hp : adhesive papillae - ivs : intra capsular seminal vesicle - lm : longitudinal muscle - m : mouth - mp : male pore - ov : ovary - p : proboscis - pg : proboscisglands - ph : pharynx - phg : pharyngial glands - r : retractor muscle - rh : rhabdites - rhg : rhabdite glands - rs : seminal receptacle - s : stylet - sta : statocyst - ut : uterus - t : testis - v : vagina - vg : prostate vesicle - vi : vitellary - vs : seminal vesicle     

Germitorosone and methylgermitorosone,two hydroanthracene derivatives from seedlings of Cassia torosa

Two new yellow pigments, germitosone and methylgermitorosone, were isolated from the seedling of Cassia torosa. The structures of these substances were established as 3,7 dimethyl - 6 - methoxy - 1 - oxo - 2,3,8,9 - tetrahydroxy - 1,2,3,4 - tetrahydroanthracene and 6,9 dimethoxy - 3,7 - dimethyl - 1 - oxo - 2,3,8 - trihydroxy - 1,2,3,4 - tetrahydroanthracene respectively.     

1,11-diarylundecan-1-one and 4-aryltetralone neolignans from Virola sebifera

The seed of Virola sebifera contains besides the polyketide 1 - (2′,6′ - dihydroxyphenyl) - 11 - henylundecan - 1 - one, four neolignans: (2S, 3S, 4R) - 4 - hydroxy - 2,3 - dimethyl - 5,6 - methylenedioxy - 4 - piperonyl - 1 - tetralone and its 2-epimer, as well as (2R, 3R, 4S) - 4 - hydroxy - 6,7 - dimethoxy - 2,3 - dimethyl 4 - piperonyl - 1 - tetralone and its (2R, 3S, 4R) - dehydroxy analogue.     

Physiological expression of macrophage apoE in the artery wall reduces atherosclerosis in severely hyperlipidemic mice

We have previously reported that the introduction of macrophage apoE into mice lacking both apoE and the LDL receptor (apoE(-)(/-)/LDLR(-)(/-)) through bone marrow transplantation (apoE(+)(/+)/LDLR(-)(/-)-->apoE(-)(/-)/LDLR(-)(/-)) produces progressive accumulation of apoE in plasma without affecting lipid levels. This model provides a tool to study the effects of physiologically regulated amounts of macrophage apoE on atherogenesis in hyperlipidemic animals. Ten-week-old male apoE(-)(/-)/LDLR(-)(/-) mice were transplanted with either apoE(+)(/+)/LDLR(-)(/-) (n = 11) or apoE(-)(/-)/LDLR(-)(/-) (n = 14) marrow. Although there were no differences between the two groups in lipid levels at baseline or at 5 and 9 weeks after transplantation, apoE levels in the apoE(+)(/+)LDLR(-)(/-)-->apoE(-)(/-)/LDLR(-)(/-) mice increased to 4 times the apoE levels of normal mice. This resulted in a 60% decrease in aortic atherosclerosis in the apoE(+)(/+)/LDLR(-)(/-)-->apoE(-)(/-)/LDLR(-)(/-) compared with the apoE(-)(/-)/LDLR(-)(/-)-->apoE(-)(/-)/LDLR(-)(/-) controls, (15957 +/- 1907 vs. 40115 +/- 8302 micro m(2) +/- SEM, respectively). In a separate experiment, apoE(+)(/+)/LDLR(-)(/-) mice were transplanted with either apoE(+)(/+)/LDLR(-)(/-) or apoE(-)(/-)/LDLR(-)(/-) marrow and placed on a high-fat diet for 8 weeks. In the absence of macrophage apoE, lesion area was increased by 75% in the aortic sinus and by 56% in the distal aorta. These data show that physiologic levels of macrophage apoE in the vessel wall are anti-atherogenic in conditions of severe hyperlipidemia and can affect later stages of plaque development.     

Effect of glutaraldehyde fixation on the localization of various oxidative and hydrolytic enzymes in the brain of rhesus monkey, Macaca mulatta

Synopsis Histochemical investigations have been made on the localization of certain oxidative and hydrolytic enzymes in the different areas of rhesus monkey brain using unfixed, freshfrozen tissue and 3% glutaraldehyde-fixed material. After glutaraldehyde fixation, the oxidative enzymes lose most of their activity normally demonstrable in the fresh-frozen section. The hydrolytic enzymes are somewhat resistant to fixation but also lose about half of the enzyme activity observed after no fixing procedure. The glycogen is better preserved in the glutaraldehyde-fixed material compared to fresh-frozen or even formaldehyde-fixed tissue. The significance of these observations is discussed in relation to glutaraldehyde as a fixative of choice in electron histochemistry.List of abbreviations used in the Figures ALH area lateralis hypothalami - APH area posterior hypothalami - AS aquaeductus Sylvii - ATN anterior thalamic nuclei - BC brachium conjunctivum - CC corpus callosum - CD nucleus caudatus - CI capsula interna - CIS cortex insularis - CM centrum medianum thalami - COR corona radiata - CP commissura posterior - CSR colliculus superior - EM eminentia medialis - F fornix - GC substantia grisea centralis - GLM corpus geniculatum laterale, magnocellular part - GLP corpus geniculatum laterale, parvocellular part - GP globus pallidus - LD nucleus lateralis dorsalis thalami - LME lamina medullaris externa thalami - LMI lamina medullaris interna thalami - LP nucleus lateralis posterior thalami - MD nucleus medialis dorsalis thalami - ML nucleus lateralis corpus mammillaris - MM nucleus medialis corpus mammillaris - NC nucleus centralis thalami - NCI nucleus colliculi inferioris - NLL nucleus lemnisci lateralis - NR nucleus ruber - NSTH nucleus subthalamicus - N III nervus oculomotorius - PC nucleus paracentralis thalami - PCR pedunculus cerebri - PUT Putamen - PV nucleus paraventricularis hypothalami - R nucleus reticularis thalami - RU nucleus reuniens thalami - SM stria medullaris thalami - SMH nucleus supramammillaris hypothalami - SMT nucleus submedius thalami - SN substantia nigra - TO tractus opticus - VL nucleus ventralis lateralis thalami - VP nucleus ventralis posterior thalami - ZI zona incerta - II ventriculus lateralis - III ventriculus tertius     

Fluorogenic substrates of glycogen debranching enzyme for assaying debranching activity

Glycogen debranching enzyme (GDE) degrades glycogen in concert with glycogen phosphorylase. GDE has two distinct active sites for maltooligosaccharide transferase and amylo-1,6-glucosidase activities. Phosphorylase limit dextrin from glycogen is debranched by cooperation of the two activities. Fluorogenic branched dextrins were prepared as substrates of GDE from pyridylaminated maltooctaose (PA-maltooctaose) and maltotetraose, taking advantage of the synthetic action of Klebsiella pneumoniae pullulanase. Their structures were as follows: Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4(Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6)Glcalpha1-4Glcalpha1-4GlcPA (B3), Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4(Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6)Glcalpha1-4Glcalpha1-4Glcalpha1-4GlcPA (B4), Glcalpha1-4Glcalpha1-4Glcalpha1-4(Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6)Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4GlcPA (B5), Glcalpha1-4Glcalpha1-4(Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6)Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4GlcPA (B6), Glcalpha1-4(Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6)Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4GlcPA (B7), and Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-6Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4Glcalpha1-4GlcPA (B8). These dextrins were incubated with porcine skeletal muscle GDE. No fluorogenic product was found in the digest of B8. The fluorogenic products from B3, B4, and B5 were PA-maltooctaose only. PA-maltooctaose, PA-maltoundecaose, and 6(7)-O-alpha-glucosyl-PA-maltooctaose were from B7. PA-maltooctaose and 6(6)-O-alpha-glucosyl-PA-maltooctaose were from B6. These results indicate that the maltooligosaccharide transferase removed the maltotriosyl residues from the maltotetraosyl branches by hydrolysis or intramolecular transglycosylation to expose 6-O-alpha-glucosyl residues, and then the amylo-1,6-glucosidase hydrolyzed the alpha-1,6-glycosidic linkages of the products rapidly. Probably, 6-O-alpha-glucosyl-PA-maltooctaoses from B7 and B6 were less susceptible to the amylo-1,6-glucosidase than were those from B3, B4, and B5. Taking this into account, B3, B4, and B5 are suitable substrates for GDE assay.     

Die abwandlung des eilegeapparates der bombyliidae (Diptera) Eine funktionsmorphologische studie

Skeleton, muscles, and functioning of the egg-laying systems of 38 species out of 16 subfamilies are studied. A development sequence toward a soil chamber to coat the eggs (b, c) can be characterized by the sternite 8: a) its inward position (Geroninae), b) its erection (9 subfamilies), c) its shell-shaped cavity (Exoprosopinae). Systropodinae and Heterotropinae cannot be fitted into this system.

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Abkürzungen in den Abbildungen VI–X 6.–10. Tergit - 6–8 6.–8. Sternit - A Analöffnung - Ac Acanthophorit 8. Sternits - Ac IX vom 9. Tergit abgetrenntes Acanthophorit - adr 1 schlauchförmige Geschlechtsanhangsdrüse - adr 2 wurstförmige Geschlechts-anhangsdrüse - Af After - AG Apikaler Gangabschnitt des R. sem. - Ak Analkegel - B IX Ausbuchtung am 9. Tergit - BG Basaler Gangabschnitt des R. sem. - Bo Borsten - Br präformierte Bruchstelle am Eideckel - Bu Bursatasche - Ch Chorion - Cö Coecum - D Darm - De Eideckel - Do Dornen - Do VIII Dornen am 8. Tergit - dR-S8 dorsaler Rand von Sternit 8 - Ed Enddarm - EmMG Einmündung der MalpighiGefäße - Er Eierrinne - Ggl 6, 7 Ganglion des 6. bzw. 7. Ab dominalsegments - Gö Geschlechtsöffnung - gOd gemeinsamer Eiergang - HaEr Haarflur entlang der Eierrinne - Ha-S8 Haare am 8. Sternit - Ha-Tv8 Haare an der Unterkante des 8. Tergits - hM häutiges Mittelfeld auf dem 8. Sternit - HPi Haarpinsel am 8. Tergit - Krs Kropfsack - Krst Kropfstiel - Ku Kufen am 8. Tergit - Lö häutige Löffelbildung - IZ lappenartige Zipfel des - M 1,2 Muskel Nr. 1, 2, etc. - MA Muskelapparat im Ductus receptaculi - Md Mitteldarm - Müadr 2 Mündung der Anhangsdrüsen-2 - MüRs Mündung der R. sem. - Od Ovidukt - Ov Ovar - PGP Postgenitalplatte - Pl Pleura - Po verdaute Pollenkörner im Enddarm - R VIII Rostrum am 8. Tergit - Rbl Rectalblase - rEi reifes Ei - Rp Rectalpapille - R. sem. Receptaculum seminis - S Sternit - Sbl Samenkapsel des R. sem. - SGP Subgenitalplatte - Sk Sandkammerbucht - Skl IX Sklerit im Dach der Sandkammerbucht - Spb Spatelborsten - Spdr Speicheldrüsen - Stg Stigma - T Tergit - Tv ventromedial verälangertes Tergit 8 - U IX, U X Zungenfortsatze des 9. bzw. 10. Tergits im Dach der Sandkammerbucht - U Ac Zungenfortsätze des Acan thophorits im Dach der Sandkammerbucht - Vag Vagina - Vag. Ap. Vaginalapodem - Vag-Es Vagina-Einsenkung in die Sternit 8-Schale - Vh Ventralhaut des Analkegels     

Arabidopsis ADC genes involved in polyamine biosynthesis are essential for seed development

Arginine decarboxylase (ADC) is a rate-limiting enzyme that catalyzes the first step of polyamine (PA) biosynthesis in Arabidopsis thaliana. We generated a double mutant deficient in Arabidopsis two ADC genes (ADC1-/- ADC2-/-) and examined their roles in seed development. None of the F2 seedlings from crosses of adc1-1 and adc2-2 had the ADC1-/- ADC2-/- genotype. In addition, some abnormal seeds were observed among the ADC1+/- ADC2-/- and ADC1-/- ADC2+/- siliques. Viable offspring with the ADC1-/- ADC2-/- genotype could not be obtained from the ADC1+/- ADC2-/- and ADC1-/- ADC2+/- plants. These results indicate that AtADC genes are required for production of polyamines that are essential for normal seed development in Arabidopsis.     

Patterns of Starchy Endosperm Acidification and Protease Gene Expression in Wheat Grains following Germination

The family of 14-3-3 proteins is ubiquitous in eukaryotes and has been shown to exert an array of functions. We were interested in the possible role of 14-3-3 proteins in seed germination. Therefore, we studied the expression of 14-3-3 mRNA and protein in barley (Hordeum distichum L.) embryos during germination. With the use of specific cDNA probes and antibodies, we could detect individual expression of three 14-3-3 isoforms, 14-3-3A, 14-3-3B, and 14-3-3C. Each homolog was found to be expressed in barley embryos. Whereas protein levels of all three isoforms were constant during germination, mRNA expression was found to be induced upon imbibition of the grains. The induction of 14-3-3A gene expression during germination was different from that of 14-3-3B and 14-3-3C. In situ immunolocalization analysis showed similar spatial expression for 14-3-3A and 14-3-3B, while 14-3-3C expression was markedly different. Whereas 14-3-3A and 14-3-3B were expressed throughout the embryo, 14-3-3C expression was tissue specific, with the strongest expression observed in the scutellum and the L2 layer of the shoot apical meristem. These results show that 14-3-3 homologs are differently regulated in barley embryos, and provide a first step in acquiring more knowledge about the role of 14-3-3 proteins in the germination process.     

Overlapping and distinct roles of STAT4 and T-bet in the regulation of T cell differentiation and allergic airway inflammation

T-bet and STAT4 play critical roles in helper T cell differentiation, especially for Th1 cells. However, it is still unknown about the relative importance and redundancy of T-bet and STAT4 for Th1 differentiation. It is also unknown about their independent role of T-bet and STAT4 in the regulation of allergic airway inflammation. In this study, we addressed these issues by comparing T-bet-deficient (T-bet(-/-)) mice, STAT4(-/-) mice, and T-bet- and STAT4-double-deficient (T-bet(-/-)STAT4(-/-)) mice on the same genetic background. Th1 differentiation was severely decreased in T-bet(-/-) mice and STAT4(-/-) mice as compared with that in wild-type mice, but Th1 differentiation was still observed in T-bet(-/-) mice and STAT4(-/-) mice. However, Th1 cells were hardly detected in T-bet(-/-)STAT4(-/-) mice. In contrast, the maintenance of Th17 cells was enhanced in T-bet(-/-) mice but was reduced in STAT4(-/-) mice and T-bet(-/-)STAT4(-/-) mice. In vivo, Ag-induced eosinophil and neutrophil recruitment into the airways was enhanced in T-bet(-/-) mice but was attenuated in STAT4(-/-) mice and T-bet(-/-)STAT4(-/-) mice. Ag-induced IL-17 production in the airways was also diminished in STAT4(-/-) mice and T-bet(-/-)STAT4(-/-) mice. These results indicate that STAT4 not only plays an indispensable role in T-bet-independent Th1 differentiation but also is involved in the maintenance of Th17 cells and the enhancement of allergic airway inflammation.     

Monoterpene ketones in the synthesis of optically active insect pheromones

This review considers the synthetic possibilities of monoterpene ketones, such as (R)-(+)- and (S)-(-)-pulegones, (-)-menthone, (R)-(-)- and (S)-(+)-carvones, (2R,5S)-dihydrocarvone, (S)-(+)- and (R)-(-)-camphors, (R)-(-)-nopinone, (R)-(+)- and (S)-(-)-verbenones by the examples of synthesis of optically pure and enantiomerically enriched insect pheromones.     

Diagnosis of genetic disease using recombinant DNA. Third edition

Summary Recombinant DNA methodology has greatly increased our knowledge of the molecular pathology of the human genome at the same time as providing the means to diagnose inherited disease at the DNA level. Direct detection and analysis of a range of genetic defects are now possible using cloned gene or oligonucleotide probes or by direct sequencing of the disease gene(s). In addition, the use of restriction fragment length polymorphisms (RFLPs) within and around these genes as indirect genetic markers has now potentiated the tracking of disease alleles in affected pedigrees in cases where direct analysis was not feasible. RFLPs associated with linked anonymous segments may also be used not only to diagnose hitherto undetectable disease states, but also for chromosomal localization of the loci responsible. We present here an updated list of reports describing both the direct and the indirect analysis/diagnosis of human inherited disease; it is intended to serve as a guide to current molecular genetic approaches in diagnostic medicine.Abbreviations ADG Annales de Genetique - AHG Ann. Hum. Genet. - AICHG Abstracts Int. Congress Hum. Genet. 7, Berlin, 1986 - AJH Am. J. Haematol. - AJHG Am. J. Hum. Genet. - AJMG Am. J. Med. Genet. - AN Aneuploidy - ANYAS Ann. New York Acad. Sci - APRT Adeninephosphoribosyltransferase - ASHG American Soc. Hum. Genet. Abstracts 34th Ann. Meeting - ATS VII Atherosclerosis VII, Eds. Fidge and Nestel, Elsevier - Arch. Neurol. Archieves of Neurology - Arch. Oph. Arch. Ophthalmol. - Atherosclr. Atherosclerosis - BBRC Biochim. Biophys. Res. Comm. - BJH Brit. J. Haematol. - BMJ Brit. Med. J. - BST Biochem. Soc. Transact. - CCG Cytogenet. Cell Genet. - CDC Carrier detection using clonality - CGC Cancer Genet. Cytogenet. - DEL Deletion - DETECT Mode of Detection - Dis. Marker Disease Markers - DUP Duplication - EJB Eur. J. Biochem. - EJI Eur. J. Immunol. - HGM8 Human Gene Mapping 8 (CCG, Vol. 40, 1–824, 1985) - HGM9 Human Gene Mapping 9 (CCG, Vol. 46, 1–824, 1987) - HGM10 Human Gene Mapping 10 (CCG, Vol. 51, 1–824, 1989) - HPRT Hypoxanthinephosphoibosyltransferase - HVR Hypervariable region - Hos. Prac. Hospital Practice - IMG Immunogenetics - INS Insertion - INV Inversion - IZ Inter-zeta - J. Mol. End. J. Molec. Endocrinol. - JAMA J. Amer. Med. Assoc. - JBC J. Biol. Chem. - JCB J. Cell. Biol. - JCEM J. Clin. Endocrinol. Metab. - JCI J. Clin. Invest. - JMD J. Inher. Metab. Dis. - JIMM J. Immunogenet. - JJCR Jpn. J. Cancer Res. - JJHG Jpn. J. Hum. Genet. - JMG J. Med.Genet. - JNR J. Neurosci. Res. - LH Loss of heterozygosity - MBM Mol. Biol. Med. - MCB Molec. Cell. Biol. - MCKUS McKusick catalogue number - MMP Mismatch pairing analysis - MODY Maturity onset diabetes of the young - MOL. END. Molec. Endocrinol. - MUT Mutation - NAR Nucl. Acids Res. - NEJM New Engl. J. Med. - Neurol. Sup. Neurology Supplement - OLIGO Detection of mutation by oligonucleotide hybridisation - OPG Ophthal. Pediatr. Genet. - PNAS Proc. Natl. Acad. Sci. USA - Ped. Res. Pediatric Res. - PM Point mutation - Pren. Diag. Prenatal Diagnosis - RE Restriction enzyme analysis - REAR Rearrangement - RFLP Indirect analysis using likned RFLP - SEQU Analysis by DNA sequencing - SCMG Somat. Cell Molec. Genet. - Thr. Res. Thrombosis Research - XIA X-inactivation analysis - ar alphoid repeat - atyp. atypical - breakp. breakpoint - def. deficiency - fruct. fructose - haem. haemoglobin - hered. hereditary - minisat. minisatellite - mt mitochondrial - neph. nephritis - persist. persistent - phosph. phosphorylase - resis. resistant - phosph. phosphorylase - resist. resistant - sev. several - synth. synthetase - var various