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S Kido K Miyamoto H Mizobuchi Y Taketani I Ohkido N Ogawa Y Kaneko S Harashima E Takeda 《The Journal of biological chemistry》1999,274(40):28256-28263
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Séguin Armand Laible G?tz Leyva Antonio Dixon Richard A. Lamb Christopher J. 《Plant molecular biology》1997,35(3):281-291
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Two plasmids containing rat thyroglobulin cDNA sequences have been constructed and characterized. A plasmid with a 500-bp insert (pRT6) was isolated and identified as thyroglobulin-specific on the basis of the tissue specificity of the inserted sequence and of its ability to retain thyroglobulin mRNA on a nitrocellulose filter. The cDNA insert in pRT6 was subsequently used to screen a rat thyroid cDNA library constructed with large cDNA. A plasmid was found containing a 1700-bp insert. The polarity and the fidelity of the insert is demonstrated by S1 mapping. 相似文献