Biomass and nutrient concentrations of sporocarps produced by mycorrhizal and decomposer fungi in Abies amabilis stands

Summary Sporocarps and sclerotia were collected for a one-year period in 23- and 180-year-old Abies amabilis stands in western Washington. All sporocarps were classified and chemically analyzed for N, P, K, Ca, Mg, Na and Fe. Lactarius sp. and Cortinarius sp. contributed the largest proportion of the total annual epigeous sporocarp production in both stands. Annual epigeous production was 34 kg/ha in the young stand and 27 kg/ha in the mature stand. Hypogeous sporocarp production increased from 1 kg ha^-1 yr^-1 to 380 kg ha^-1 yr^-1 with increasing stand age. High sclerotia biomass occurred in the young (2,300 kg/ha) and mature (3,000 kg/ha) stands. Peak sclerotia and epigeous sporocarp biomass in the young stand and epigeous and hypogeous sporocarp biomass in the mature stand coincided with the fall peak of mycorrhizal root biomass.In the young stand, sporocarps produced by decomposer fungi concentrated higher levels of Ca and Mn than those produced by mycorrhizal fungi. In the mature stand, sporocarps of decomposer fungi concentrated higher levels of N, P, Mn, Ca and Fe than sporocarps of mycorrhizal fungi. Epigeous and hypogeous sporocarps concentrated higher levels of N, P, and K than sclerotia or mycelium. The highest concentration of N (4.36%), P (0.76%), K (3.22%) and Na (1,678 ppm) occurred in epigeous sporocarps. Highest Mn (740 ppm) and Ca (20,600 ppm) concentrations occurred in mycelium, while highest Mg (1,929 ppm) concentrations were in hypogeous sporocarps and highest Fe (4,153 ppm) concentrations were in sclerotia.     

Nitrogen fixation by Spirillum sp. from rice roots

Nitrogen fixation by Spirillum sp. obtained from the roots of rice plants grown at different levels of combined nitrogen was studied. The roots of rice plants exposed to low levels of combined nitrogen (20–40 kg N/ha) harboured Spirillum sp. possessing higher nitrogen-fixing efficiency as compared to the cultures from plants receiving 60–100 kg N/ha. More-over, the nitrogen-fixing efficiency of these Spirillum spp. varied with age of the plant, irrespective of the dosage of combined nitrogen.     

Nitrogen fixation by non-legumes in tropical agriculture with special reference to wetland rice

Summary Of the 143 million hectares of cultivated rice land in the world, 75% are planted to wetland rice. Wet or flooded conditions favour biological nitrogen fixation by providing (1) photic-oxic floodwater and surface soil for phototrophic, free-living or symbiotic blue-green algae (BGA), and (2) aphotic-anoxic soil for anaerobic or microaerobic, heterotrophic bacteria. TheAzolla-Anabaena symbiosis can accumulate as much as 200 kg N ha^–1 in biomass. In tropical flooded fields, biomass production from a singleAzolla crop is about 15 t fresh weight ha^–1 or 35 kg N ha^–1. Low tolerance for high temperature, insect damage, phosphorus requirement, and maintenance of inoculum, limit application in the tropics. Basic work on taxonomy, sporulation, and breeding ofAzolla is needed. Although there are many reports of the positive effect of BGA inoculation on rice yield, the mechanisms of yield increase are not known. Efficient ways to increase N₂-fixation by field-grown BGA are not well exploited. Studies on the ecology of floodwater communities are needed to understand the principles of manipulating BGA. Bacteria associated with rice roots and the basal portion of the shoot also fix nitrogen. The system is known as a rhizocoenosis. N₂-fixation in rhizocoenosis in wetland rice is lower than that ofAzolla or BGA. Ways of manipulating this process are not known. Screening rice varieties that greatly stimulate N₂-fixation may be the most efficient way of manipulating the rhizocoenosis. Stimulation of N₂-fixation by bacterial inoculation needs to be quantified.     

Nitrate reductase activity,biomass, yield,and quality in cotton in response to nitrogen fertilization

In the production of cotton (Gossypium hirsutum L.), nitrogen fertilization is one of the most costly crop practices, but important to reach high yields. However, high nitrogen (N) content in plants does not always translate into a high fibre production. One way of assessing the efficiency of the N fertilizer is through the enzymatic activity of the nitrate reductase (NR). This is a key enzyme in N assimilation, whose activity is regulated by a number of endogenous and exogenous factors that determine yield. The aim of this study was to assess the effect of N fertilization on yield, fibre quality, biomass, and NR enzymatic activity in vivo in the cotton variety Fiber Max 989. The evaluated application rates were 0, 50, 100, and 150 kg/ha of N, using urea as a source (46% N) in a randomizedblock design with three replicates. At harvest, the maximum yield of seed cotton and the greatest accumulation of total foliar biomass through time was reached after applying 150 kg N/ha. The different N-application rates did not affect the components of cotton-fibre quality. The activity of endogenous NR was greater on plants where 150 kg N/ha were applied. The highest cotton yield and N contents were obtained on these plants. Therefore, the NR activity in vivo could be used as a bioindicator of the N nutritional level in cotton.     

Nitrogen fixation by Myrica gale root nodules Massachusetts wetland

Summary Nitrogenase activity was measured by acetylene reduction in excised Myrica gale nodules collected throughout the growing season at two sites associated with a small lake in central Massachusetts. One site was in an open, weakly minerotrophic peatland dominated by M. gale and the other was on the lakeshore. Nitrogenase activity appeared in late May when the leaves unfolded, reached a peak in July when the maximum number of leaves was present, and declined until it was no longer measurable in late October several days after all leaves had fallen. Summer activities were substantially higher at the peatland site than the lakeshore. Maximum activities were 19.8±1.9 and 8.1±0.7 mol/h x g dry weight (x±SE; N=20) at the peatland and lakeshore sites respectively.Nitrogenase activities were very low at 5° C and increased linearly from 10 to 30° C, the highest temperature examined. The maximum soil temperature measured was 20° C, and no significant diurnal fluctuation in activity was detected.Annual nitrogen fixation calculated from the seasonal nitrogenase activity curve was 34 kg N/ha x yr at the peatland site with mean dry weight nodule biomass of 104 kg/ha, and 24 kg N/ha x yr at the lakeshore with 111 kg/ha nodule biomass. These rates of nitrogen fixation are equivalent to 4–5x the amount of nitrogen contained in bulk precipitation and are major components in the nitrogen budgets of the M. gale plants and wetlands in which they grow.     

The effect of stem and cob borers on maize subjected to different nitrogen treatments

The influence of four nitrogen levels (0, 60, 90 and 120 kg N/ha) on growth of maize and development of lepidopterous pests was investigatdd in a field trial. Nitrogen had a positive effect on both plant growth variables (plant height, stem diameter and yield), and development and survival ofSesamia calamistis andEldana saccharina, and thereby increased the incidence of dead hearts and stem tunneling. However, the percent yield loss due to artificial infestation decreased with increasing N application rate from 20% to 11% in the in the 0kg/ha and 120kg/ha treatment, respectively. Using a multiple regression analysis, plant height, plant diameter and stem tunneling were found to be the most important variables explaining the variability in maize yield.     

How does plant population density affect the biomass of Ravenna grass?

Ravenna grass, Tripidium ravennae (L.) H. Scholz, is known to produce an abundance of biomass, but how plant density affects its biomass potential remains unknown. The objectives were to determine the effects of plant density on biomass yield; plant growth traits; biomass?carbon, nitrogen, and ash concentrations; heating value; nitrogen removal; and sucrose concentration in leaves and culms. The treatments consisted of five plant densities (1,250; 2,500; 5,000; 10,000; and 20,000 plants per hectare) in a randomized complete block design with four blocks. Plots were nonirrigated, unfertilized, and harvested once during the dormant season each year. Data were collected from 2015?2019. Dependent variables that varied with plant population density (p < .05) were biomass yield, number of reproductive culms per plant, reproductive culm diameter, reproductive culm sucrose concentration, and nitrogen removal with biomass. Biomass yield ranged from 5.6 to 16.3 Mg/ha for plant densities of 1,250–20,000 plants per hectare, respectively. Combined over years, nonlinear regression of the data showed the equation for biomass yield to plateau at 16.2 Mg/ha at a plant density of 10,640 plants per hectare. As plant density increased, the number of reproductive culms per plant, culm diameter, and culm sucrose concentration significantly decreased. At 1,250 plants per hectare, the number of reproductive culms per plant, culm diameter, and culm sucrose averaged 70, 10.2 mm, and 63.2 g/kg, respectively. Nitrogen removed with biomass significantly increased as biomass yield increased with plant density. At a density of 10,000 and 20,000 plants per hectare, the amount of nitrogen removed annually in the harvested biomass averaged 88 kg/ha. The data suggest that 10,000 plants per hectare would produce the greatest annual biomass yields; however, research is needed to determine the nutrient requirement for Ravenna grass to sustain biomass production at that density.     

Preliminary results of a fertilization-irrigation experiment in a Quercus ilex L. forest in relation to leaves and twigs characteristics

A multifactorial ANOVA experiment was designed for a Quercus ilex forest in the Prades mountains in Tarragona (NE Spain). The purpose of this study was to clarify the relative importance of water, nitrogen and phosphorus as factors controlling primary production.Eight plots comprising all possible combinations of the three factors (including a control) were laid out in three replicate blocks. Fertilizers were applied to the appropriate treatments in March 1989 with a dosage of 125 kg of P/ha and 250 kg N/ha. Irrigation was applied with a rate of 20 mm per week during the warm season.To evaluate the effects on canopy structure we measured specific leaf weight (mg/cm²), average leaf weight (mg), average twig height and average twig length. These variables were selected as they are the most likely to manifest changes over short time periods following changes in environmental conditions.The values of these parameters before initial treatments (autumn 1988) and after treatments (summer 1990) were analyzed. The results obtained show that water and nitrogen have a greater effect than phosphorus on tree canopy structure, at least during initial development.     

The effect of Rhizobium inoculation and nitrogen fertiliser on nitrogen fixation and seed yield of dry beans (Phaseolus vulgaris)

The potential benefit to be derived from seed inoculation of Phaseolus vulgaris beans with effective strains of Rhizobium phaseoli, was investigated in field experiments over three years on a site low in soil nitrogen and lacking indigenous effective strains of R. phaseoli. Inoculation with R. phaseoli (strain RCR 3644) produced significant increases in nodulation, nitrogenase activity and plant growth in all experiments. In trials in 1978 and 1979, with cv. Seafarer, inoculation, in the absence of nitrogen fertiliser doubled seed yields. In 1978, the seed yields from inoculated beans without nitrogen fertiliser (1–6 t/ha) were not significantly different from those obtained with uninoculated beans receiving the optimum nitrogen fertiliser treatment of 120 kg N/ha (1–75 t/ha). In 1979, with lower rainfall favouring more efficient utilisation of nitrogen fertiliser, inoculation gave seed yields (1–88 t/ha) equivalent to those obtained with 60 kg N/ha (1–70 t/ha) but significantly less than with 120 kg N/ha (2–88 t/ha). More precise estimates from nitrogen response curves showed that inoculation supplied the fertiliser equivalent of 105 and 70 kg N/ha in 1978 and 1979 respectively. In both years, significant benefits were also obtained by the combination of inoculation and nitrogen fertiliser. In a separate experiment in 1979, with four R. phaseoli strains inoculated onto eight bean cultivars, three were highly effective nitrogen fixers on all cultivars. Two strains (RCR 3644 and NVRS 963A) each increased mean yields, in the absence of nitrogen fertiliser, from 1–39 t/ha uninoculated to c. 2–5 t/ha inoculated whilst strain RCR 3622 was outstanding with a mean yield of 3-0 t/ha. An analysis of the nitrogen content of seed showed that gains from nitrogen fixation were 37–57 kg N/ha/growing cycle for the combination RCR 3644 with cv. Seafarer. However, 106 kg N/ha/growing cycle was recorded for the combination RCR 3622 and cv. Aurora.     

Impact of warm‐season grass management on feedstock production on marginal farmland in Central Illinois

The production of dedicated energy crops on marginally productive cropland is projected to play an important role in reaching the US Billion Ton goal. This study aimed to evaluate warm‐season grasses for biomass production potential under different harvest timings (summer [H1], after killing frost [H2], or alternating between two [H3]) and nitrogen (N) fertilizer rates (0, 56, and 112 kg N/ha) on a wet marginal land across multiple production years. Six feedstocks were evaluated including Miscanthus x giganteus, two switchgrass cultivars (Panicum virgatum L.), prairie cordgrass (Spartina pectinata Link), and two polycultures including a mixture of big bluestem (Andropogon gerardii Vitman), indiangrass (Sorghastrum nutans), and sideoats grama (Bouteloua curtipendula [Michx.] Torr.), and a mixture of big bluestem and prairie cordgrass. Across four production years, harvest timing and feedstock type played an important role in biomass production. Miscanthus x giganteus produced the greatest biomass (18.7 Mg/ha), followed by the switchgrass cultivar “Liberty” (14.7 Mg/ha). Harvest in H1 tended to increase yield irrespective of feedstock; the exception being M. x giganteus that had significantly lower biomass when harvested in H1 when compared to H2 and H3. The advantage H1 harvest had over H2 for all feedstocks declined over time, suggesting H2 or H3 would provide greater and more sustainable biomass production for the observed feedstocks. The N application rate played an important role mainly for M. x giganteus where 112 kg N/ha yielded more biomass than no N. Other feedstocks occasionally showed a slight, but statistically insignificant increase in biomass yield with increasing N rate. This study showed the potential of producing feedstocks for bioenergy on wet marginal land; however, more research on tissue and soil nutrient dynamics under different N rates and harvest regimes will be important in understanding stand longevity for feedstocks grown under these conditions.     

Soil and litter respiration rates in different microhabitats of a mixed oak-conifer forest and their control by edaphic conditions and substrate quality

Summary Seven isolates belonged toA. brasilense and 3 belonged toA. lipoferum. Isolates having more denitrifying capacity fixed less nitrogen in nitrogen free semi-solid malate medium. One strain ofA. lipoferum having high nitrogen fixing capacity with negative test for denitrification was tested as inoculant to supplement the nitrogen need of a wheat crop in field condition with different doses of N with and without the inoculant. While control without nitrogen yielded 1260 kg/ha the yield in inoculated treatment was 2070 kg/ha resulting in significant increase. In a treatment receiving 40 kg N/ha the grain yield was 2370 kg/ha as against yield of 3110 kg/ha in a similar treatment receiving fertiliser plus inoculant. Thus increase in yield was about 30%. Further the treatment receiving 80 kg N/ha yielded 2970 kg/ha as against yield of 4150 kg/ha in a treatment receiving inoculant alongwith the above dose of the fertiliser. Thus increase in yield due to application of inoculant was about 36%. Similarly, the uptake of N in different treatments was augmented due to inoculation of seeds with the culture.     

Long-term integrated soil fertility management in South-western Nigeria: Crop performance and impact on the soil fertility status

Crop response, tree biomass production and changes in soil fertility characteristics were monitored in a long-term (1986–2002) alley-cropping trial in Ibadan, Nigeria. The systems included two alley cropping systems with Leucaena leucocephala and Senna siamea on the one hand and a control (no-trees) system on the other hand, all cropped annually with a maize–cowpea rotation. All systems had a plus and minus fertilizer treatment. Over the years, the annual biomass return through tree prunings declined steadily, but more drastically for Leucaena than for Senna. In 2002, the nitrogen contribution from Leucaena residues stabilized at about 200 kg N/ha/year, while the corresponding value for Senna was about 160 kg N/ha/year. On average, the four Leucaena prunings were more equal in biomass as well as in amounts of N, P and cations, while the first Sennapruning was always contributing up to 60% of the annual biomass or nutrient return. Maize crop yields declined steadily in all treatments, but the least so in the Senna + fertilizer treatment where in 2002 still 2.2 tonnes/ha of maize were obtained. Nitrogen fertilizer use efficiency was usually higher in the Senna treatment compared to the control or the Leucaena treatment. Added benefits due to the combined use of fertilizer N and organic matter additions were observed only for the Sennatreatment and only in the last 6 years. At all other times, they remained absent or were even negative in the Leucaenatreatments for the first 3 years. Most chemical soil fertility parameters decreased in all the treatments, but less so in the alley cropping systems. The presence of trees had a positive effect on remaining carbon stocks, while they were reduced compared to the 1986 data. Trees had a positive effect on the maintenance of exchangeable cations in the top soil. Exchangeable Ca, Mg and K – and hence ECEC – were only slightly reduced after 16 years of cropping in the tree-based systems, and even increased in the Senna treatments. In the control treatments, values for all these parameters reduced to 50% or less of the original values after 16 years. All the above points to the Senna-based alley system with fertilizers as the more resilient one. This is reflected in all soil fertility parameters, in added benefits due to the combined use of fertilizer nitrogen and organic residue application and in a more stable maize yield over the years, averaging 2.8 tonnes/ha with maximal deviations from the average not exceeding 21%.     

Gulf Coast Riceland Seed Biomass Estimates for Waterfowl Habitat Conservation

Biomass estimates of potential waterfowl foods are fundamental to estimating foraging carrying capacity of waterfowl habitat by conservation planners and managers of the North American Waterfowl Management Plan-Gulf Coast Joint Venture (GCJV). Rice and moist-soil seeds in Gulf Coast rice fields provide principal sources of energy for waterfowl during migration and winter. We investigated spatio-temporal biomass dynamics of these seeds and modeled their variation in production and idled rice fields in southwestern Louisiana, southeastern Texas, and the Texas Mid-Coast, USA, in August and November 2010–2013. We hypothesized that previous estimates of November rice and moist-soil seed biomass from the Mississippi Alluvial Valley were not applicable to the GCJV region because climate and agricultural production practices (e.g., ratooning, crayfish [Procambrus spp.] aquaculture) are primary inter-regional contrasts. Waste-rice biomass was greatest in November in fields with an unharvested second crop of rice from tillers of original plants (i.e., ratoon crop; 837.7 kg[dry]/ha; CV = 16.7%) and least in fields without a ratoon crop (119.3 kg/ha; CV = 18.5%). Moist-soil seed biomass was greatest in idled rice fields in October (477.3 kg/ha; CV = 24.8%), where substrate and rice stubble were disked at the time of sampling, and in idled fields with standing native vegetation in November (304.8 kg/ha; CV = 17.1%). Field-level variation in waste rice in production fields in November was best explained by an interaction between field management (e.g., harvested ratoon) and rice variety. We were unable identify a reliable predictor of field-level variation in moist-soil seed biomass in idled fields for July–August or November (i.e., null model was best or competitive). Substituting existing seasonal moist-soil and rice seed biomass estimates in GCJV planning models with those from our study would result in a seasonally flooded habitat objective 76% (101,974 ha) greater than the current GCJV estimate for 3 rice-growing planning areas. We encourage conservation planners in the GCJV region to use biomass estimates from our study because they are reasonably precise for planning and implementation (i.e., CV ~ 20%) and represent most contemporary patterns of farming practices and food abundance in this region. Further, programs and incentives that promote production of ratoon rice crops and allow growth of naturally occurring vegetation in idled rice fields, followed by shallow flooding during November–February, would significantly enhance food resources for waterfowl and other waterbirds in this important landscape for North American avifauna. © 2020 The Wildlife Society.     

Bacterial Community Succession in Natural River Biofilm Assemblages

Temporal bacterial community changes in river biofilms were studied using 16S rRNA gene-based polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) followed by sequence analysis. Naturally occurring biofilms were sampled in 2001 during an undisturbed 7-month low-water period in the River Garonne (SW France). During the sampling period epilithic biomass exhibited a particular pattern: two 3-month periods of accumulation that resulted in two peaks in summer and fall, each at about 25 g ash-free dry mass per square meter. Bacterial community DGGE profiles differed between the summer and fall biomass peaks and shared only 30% common operational taxonomic units (OTUs), suggesting the influence of seasonal factors on these communities. During the second biomass accrual phase, bacterial richness and the appearance of new OTUs fitted a conceptual model of bacterial biofilm succession. During succession, five OTUs (corresponding to Dechloromonas sp., Nitrospira sp., and three different Spirosoma spp.) exhibited particular patterns and were present only during clearly defined successional stages, suggesting differences in life-history strategies for epilithic bacteria. Co-inertia analysis of DGGE banding patterns and physical–chemical data showed a significant relationship between community structure and environmental conditions suggesting that bacterial communities were mainly influenced by seasonal changes (temperature, light) and hydrodynamic stability. Within the periods of stability, analysis of environmental variables and community patterns showed the dominant influence of time and maturation on bacterial community structure. Thus, succession in these naturally occurring epilithic biofilm assemblages appears to occur through a combination of allogenic (seasonal) and autogenic changes.     

Biomass and elemental uptake in fertilized and unfertilizedBetula papyrifera Marsh. andPopulus grandidentata Michx.

Summary Estimates were made of the above-ground biomass and contents of N, P, K, Ca, Mg, Mn, Na, Fe, Zn, Al, and Cu in fertilized (N 448 kg/ha, P 112 kg/ha, lime 4480 kg/ha) and unfertilized white birch (Betula papyrifera Marsh.) and bigtooth aspen (Populus grandidentata Michx.). For individuals of both species, fertilization increased the average above-ground biomass increment and the N and P content increment by 150 per cent and 300 per cent, respectively, but decreased uptake of Mn and Zn. The allocation of biomass and elements differs not only between species, but within species under untreated and fertilized conditions.     

九龙江口红树林研究IV.秋茄群落的氮、磷的累积和循环

本文是福建九龙江口红树林生态系统研究的一个部分,主要讨论20年生秋茄群落的氮、磷含量及其生物循环。试验结果表明：秋茄群落现存量中,含有氮、磷总量分别为935.47和112.02公斤／公顷。其中地上部分别为582.26和70.47公斤／公顷,地下部分别为353.21和41.55公斤／公顷。该群落氮、磷元素生物循环中,年吸收量分别为213.31和21.75公斤／公顷;存留量分别为83.75和10.91公斤／公顷;归还量分别为129.52和10.84公斤／公顷。它们的氮含量均大于磷含量,周转期氮需7年比磷需10年为快。     

Young secondary vegetation and soil interactions in Izabal,Guatemala

Summary A study of the relationships of soils and vegetation was undertaken in the humid tropical region of Lake Izabal, Guatemala, Central America. Soils and associated 10-month-old secondary growth were analyzed for N, P, K, Ca, and Mg. In addition, organic matter, Al and pH were determined in the soil samples and plant biomass for the secondary growth were calculated. The secondary growth biomass averaged 9,710 kg/ha for the 10-month growth period. Total nutrient content of the vegetation increased linearly with the biomass, except for Mg. The antagonism of Mg on K and Ca nutrition was quite significant when Mg exceeded Ca in the soil under shifting cultivation. Pure stands ofHeliconia sp. andGynerium sp. appeared to be more efficient in accumulating P than stands of mixed vegetation. Chemical composition and dry matter production of native vegetation may provide additional information to evaluate soil fertility in the humid tropics.     

Effect of rates of nitrogen and relative efficiency of sulphur-coated urea and nitrapyrin-treated urea in dry matter production and nitrogen uptake by rice

Summary A field experiment conducted for two rainy seasons (1974 and 1975) on a sandy clay loam soil at the Indian Agricultural Research Institute, New Delhi showed that at 100kg N/ha the apparent recovery of urea nitrogen by the rice crop was only 28%, which was raised to 41.7% by treating urea with Nitrapyrin and to 47.4% by coating urea withneem (Azadirachta indica Juss) cake. The recovery with sulphur-coated urea was 37.7%. Dry matter production nitrogen concentration in plant and uptake by rice were increased as the rate of nitrogen was increased from 0 to 150kg N/ha. Advantage of treating urea with Nitrapyrin or coating withneem cake was seen more in grain than straw yield.     

Diurnal and seasonal patterns of nitrogen fixation in analnus hirsuta plantation of central Korea

We used an acetylene reduction assay to measure rates of nitrogen fixation on a 38-year-oldAlnus hirsuta plantation in central Korea. The diurnal pattern of acetylene reduction changed significantly during May, August, and October, typically varying by 3-fold throughout the course of the day. Maximum rates occurred at 3 p.m. in May and October, but at 6 p.m. in August. Increasing trends were evident during the early growing season, with sustained high rates from mid-May through late September; July had the highest rates, averaging 7.2 μmole g^-1 dry nodule h^-1. The average nodule biomass for this plantation was 220 kg ha ’. Rates of acetylene reduction were related to soil temperature, but not to soil moisture content. Combining these nodule biomass calculations with seasonal average acetylene reduction rates yielded an estimate of current annual nitrogen fixation of 60 kg N ha^-1 for the plantation. This rate of annual nitrogen addition was very large in relation to the yearly nitrogen requirements of coniferous and deciduous forests in central Korea.     

Soil nitrogen dynamics in a holm oak forest

Soil nitrogen (N) dynamics were studied in a dense, holm oak (Quercus ilex ssp. ilex) stand in the Montseny mountains to determine annual and seasonal patterns of N availability and uptake in an undisturbed Mediterranean forest on acidic soil. Soil mineral N content, net N mineralization (NNM), and net nitrification (NN) were determined by monthly sampling at two soil depths followed by in situ incubation in polyethylene bags. NNM per unit of soil mass was much higher at 0–5 cm than at 5–20 cm (annual means 24 and 2.5 mg N/kg, respectively) but on an area basis NNM was similar at both depths. A total of 80 kg N/ha/yr were mineralized from the first 20 cm of soil. NN amounted to only 9% of the annual NNM (7.5 kg N/ha/yr) and it occurred only in the upper 5 cm. NNM was maximum in June and July, while the NN peaked in May. Despite favourable soil temperature and moisture, NNM was negative in autumn because of microbial immobilization. Seasonal and depth variations of NNM appeared to be controlled more by substrate quality than by organic matter quantity, temperature or moisture. NN was not limited by ammonium availability. Calculated N uptake amounted to 91 kg/ha yr, peaking in June and July. The investigated stand showed a moderately high N availability, but ammonium was the major form of mineral N supply for holm oak.