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1.
A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5 T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5 T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979 T (98.4 %) and Deinococcus daejeonensis KCTC 13751 T (97.5 %). Strain N5 T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D 10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C 16:1 ω7c (25.25 %), C 15:1 ω6c (19.77 %), C 17:1 ω6c (11.87 %), and C 17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5 T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5 T (=KCTC 33153 T = JCM 19176 T). 相似文献
2.
Strain 16F3H T, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3H T was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘ Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314 T (98.1%) and Deinococcus caeni Ho-08 T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3H T possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C 15:1 ω6 c and C 16:1 ω7 c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3H T (=KCTC 33794 T = JCM 31406 T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed. 相似文献
3.
Strain 16F1E T was isolated from a 3-kGy-irradiated sediment sample collected at Han River in Seoul, Republic of Korea. Cells of this strain were observed to be Gram-positive, pililike structure, and short rod shape, and colonies were red in color. The strain showed the highest degree of 16S rRNA gene sequence similarity to Deinococcus aquaticus PB314 T (98.8%), Deinococcus depolymerans TDMA-24 T (98.1%), Deinococcus caeni Ho-08 T (98.0%), and Deinococcus grandis DSM 3963 T (97.0%). 16S rRNA gene sequence analysis identified this strain as a member of the genus Deinococcus (Family: Deinococcaceae). The genomic DNA G+C content of strain 16F1ET was 66.9 mol%. The low levels of DNA-DNA hybridization (< 56.2%) with the species mentioned above identified strain 16F1E T as a novel Deinococcus species. Its oxidase and catalase activities as well as the production of acid from glucose were positive. Growth of the strain was observed at 10–37°C (optimum: 20–30°C) and pH 4–10 (optimum: pH 7–8). The cells tolerated less than 5% NaCl and had low resistance to gamma radiation (D 10 < 4 kGy). Strain 16F1ET possessed the following chemotaxonomic characteristics: C 16:0, C 15:1ω6 c, and C 16:1ω7 c as the major fatty acids; phosphoglycolipid as the predominant polar lipid; and menaquinone-8 as the predominant respiratory isoprenoid quinone. Based on the polyphasic evidence, as well as the phylogenetic, genotypic, phenotypic, and chemotaxonomic characterization results, strain 16F1E T (=KCTC 33793 T =JCM 31404 T) is proposed to represent the type strain of a novel species, Deinococcus seoulensis sp. nov. 相似文献
4.
A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1 T, was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m ?2). Based on 16S rRNA gene sequence comparisons, strain 6.1 T shows high similarity to Deinococcus citri DSM 24791 T (98.1%) and Deinococcus gobiensis I-0 T (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C 16:1 ω7 c and/or iso-C 15:0 2-OH), followed by iso-C 17:1 ω9 c and C 16:0. The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA–DNA hybridization between strain 6.1 T and D. citri DSM 24791 T was 45.6 ± 7.1% and with D. gobiensis I-O T was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1 T represents a novel species of the genus Deinococcus, for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1 T (= CGMCC 1.15053 T = KCTC 33744 T). 相似文献
5.
Two Gram-negative, non-spore-forming, oval to pear shaped motile strains, designated 25B14_1 T and BH-BN04-4 T, isolated from surface seawater from the Bering Sea and Chukchi Sea, respectively, were subjected to polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strains 25B14_1 T and BH-BN04-4 T clustered together with Hyphomonas atlanticus 22II1-22F38 T and Hyphomonas oceanitis DSM 5155 T, respectively, within genus Hyphomonas. Based on whole genome sequence analysis, the calculated DDH and ANIm values between strain 25B14_1 T and BH-BN04-4 T are 18.8 and 83.19 % respectively. The calculated DDH values of strain 25B14_1 T and BH-BN04-4 T with seven type strains ranged from 18.2 to 19.9 % and from 18.4 to 40.4 %, respectively. The ANIm values of strain 25B14_1 T and BH-BN04-4 T with seven type strains ranged from 83.00 to 84.67 % and from 83.14 to 90.58 %, respectively. Both isolates were found to contain Q-11 as the predominant respiratory quinone. The major fatty acids of strain 25B14_1 T were identified as C 16:0, C 17:0, C 18:1 ω7 c-methyl and Summed Feature 8 (C 18:1 ω6 c/ ω7 c as defined by MIDI), while in the case of strain BH-BN04-4 T they were identified as C 16:0, C 18:1 ω7 c-methyl and Summed Feature 8 (C 18:1 ω6 c/ ω7 c). The G+C contents of 25B14_1 T and BH-BN04-4 T were determined to be 58.4 and 61.0 mol%, respectively. The combined phenotypic and genotypic data show that the two isolates each represent novel species of the genus Hyphomonas, for which the names Hyphomonas beringensis sp. nov. and Hyphomonas chukchiensis sp. nov. are proposed, with the type strain 25B14_1 T (=MCCC 1A07321 T = LMG 27914 T) and BH-BN04-4 T (=MCCC 1A07481 T = LMG 27915 T), respectively. 相似文献
6.
A taxonomic study was carried out on strain 22II-S10s T, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5–18 % (optimum, 2–12 %), at pH of 3–10 (optimum, 7) and at temperatures of 10–41 °C (optimum 28 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10s T belongs to the genus Roseivivax, with highest sequence similarity to Roseivivax halodurans JCM 10272 T (97.2 %), followed by Roseivivax isoporae LMG 25204 T (97.0 %); other species of genus Roseivivax shared 95.2–96.7 % sequence similarity. The DNA–DNA hybridization estimate values between strain 22II-S10s T and the two type strains ( R. halodurans JCM 10272 T and R. isoporae LMG 25204 T) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C 18:1 ω7 c/ ω6 c) (67.4 %), C 18:0 (7.2 %), C 19:0 cyclo ω8 c (7.1 %), C 18:1 ω7 c 11-methyl (6.8 %) and C 16:0 (5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10s T represents a novel species within the genus Roseivivax, for which the name Roseivivax atlanticus sp. nov. is proposed, with the type strain 22II-S10s T (= MCCC 1A09150 T = LMG 27156 T). 相似文献
7.
A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1L T was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27 T (99.0%), D. grandis DSM 3963 T (98.1%), D. radiotolerans C1 T (97.5%), and D. caeni Ho-08 T (97.2%). Strain 16F1L T was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1L T was 67.2 mol%. Strain 16F1L T was found to grow at temperatures of 10–37°C (optimum 25°C) and pH 7–8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1L T showed resistance to gamma radiation (D 10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C 15:1ω6 c and C 16:1ω7 c; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1L T represents a novel species within the genus Deinococcus, for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1L T (=KCTC 33796 T =JCM 31405 T). 相似文献
8.
A Gram-negative, aerobic, non-motile bacterial strain hun6 T isolated from the polluted soil near a chemical factory in northern Nanjing, China was investigated to clarify its taxonomic position. Growth of strain hun6 T occurred between 10 and 45 °C (optimum, 30 °C) and between pH 6.0 and 8.0 (optimum, pH 7.0). No growth occurred at NaCl concentrations greater than 5 % (w/v). The 16S rRNA gene sequence analysis indicated that strain hun6 T belongs to the genus Aquamicrobium. The sequence similarities of strain hun6 T to other type strains of Aquamicrobium genus were all below 98.5 %. The presence of ubiquinone-10, the predominant fatty acid summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c) and C 19:0 cyclo ω8c, a polar lipid pattern with phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phophatidylmonomethylethanoamine were in accord with the characteristics of the genus Aquamicrobium. The G+C content of the genomic DNA was determined to be 63.5 mol%. The results of DNA–DNA hybridization, physiological and biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of strain hun6 T from all known Aquamicrobium species. Therefore, strain hun6 T can be assigned to a new species of this genus for which the name Aquamicrobium terrae sp. nov. is proposed. The type strain is hun6 T (= CICC 10733 T = DSM 27865 T). 相似文献
9.
Two moderately halophilic, facultatively aerobic, motile bacteria with flagella, designated strains 10-C-3 T and 30-C-3, were isolated from jeotgal, a traditional Korean fermented seafood. Cells of the strains were observed to be ovoid-rods showing catalase- and oxidase-positive reactions and production of creamy-pink pigments. Growth of strain 10-C-3 T was observed at 15–35 °C (optimum, 25–30 °C), at pH 5.5–9.0 (optimum, pH 7.0–7.5), and in the presence of 3–15 % (w/v) salts (optimum: 5–10 %). The two strains were found to contain C 18:1 ω7 c, C 16:0, summed feature 3 (as defined by the MIDI system, comprising C 16:1 ω7 c and/or C 16:1 ω6 c), and C 12:0 3-OH as the major cellular fatty acids. The G+C contents of the genomic DNA of strains 10-C-3 T and 30-C-3 were determined to be 63.2 and 63.1 mol%, respectively and the respiratory quinone detected was ubiquinone 9 (Q-9) only. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 10-C-3 Tand 30-C-3 formed a distinct phyletic lineage within the genus Halomonas and are most closely related to Halomonas fontilapidosi 5CR T with 95.2 % of 16S rRNA sequence similarity. Strains 10-C-3 Tand 30-C-3 shared 99.2 % of 16S rRNA gene sequence similarity and their DNA–DNA relatedness value was 96.6 ± 0.9 %. On the basis of phenotypic, chemotaxonomic and molecular features, strains 10-C-3 Tand 30-C-3 represent a novel species of the genus Halomonas, for which the name Halomonas cibimaris sp. nov. is proposed. The type strain is 10-C-3 T (= KACC 14932 T = JCM 16914 T). 相似文献
10.
A Gram-negative, short rod-shaped, floc-forming bacterial strain J5-66 T without any flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was capable of optimal growth at pH 7, 30 °C, and 1–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the genus Ottowia in Comamonadaceae, and the highest 16S rRNA gene sequence similarity was 96.2 % with Ottowia pentelensis DSM 21699 T. The major cellular fatty acids of strain J5-66 T were C 16:1 ω7 c/C 16:1 ω6 c (45.0 %), C 16:0 (21.1 %), C 18:1 ω7 c or/and C 18:1 ω6 c (19.2 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, glycolipid and two unidentified phospholipids (PL1 and PL2). The predominant ubiquinone was Q-8, and the G+C content of the genome DNA was 64.4 mol%. On the basis of genetic, phenotypic and chemotaxonomic analyses, strain J5-66 T represents a novel species of the genus Ottowia for which the name Ottowia shaoguanensis sp. nov. is proposed. The type strain is J5-66 T (=CGMCC 1.12431 T =LMG 27408 T). 相似文献
11.
A Gram-stain negative, ovoid-rod shaped, strictly aerobic bacterium, strain Y2 T, was isolated from a deep-sea sediment of the Western Pacific. Phylogenetic and phenotypic properties of the organism indicated that it belongs to the genus Altererythrobacter. Strain Y2 T shares highest 16S rRNA gene sequence similarity of 96.6 % with Erythrobacter jejuensis CNU001 T, followed by the type strains of recognized members of the genus Altererythrobacter (94.8–96.5 %). Strain Y2 T forms a clade with E. jejuensis CNU001 T in the cluster of species of the genus Altererythrobacter. Growth of strain Y2 T was observed at 4–40 °C (optimum, 35–37 °C), at pH 6.0–10.0 (optimum, pH 7.0–8.0) and in the presence of 0–5 % (w/v) NaCl (optimum, 2–3 %). The major cellular fatty acids were found to be C 17:1 ω6c (41.5 %), summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c; 17.2 %), C 17:1 ω8 c (11.0 %) and C 15:0 2OH (8.1 %). The major respiratory quinone was determine to be ubiquinone 10 (Q-10). The polar lipid analysis indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, one sphingoglycolipid, three unidentified phospholipids, two unidentified glycolipids, two unidentified aminolipids and three unknown lipids. The DNA G + C content of the type strain is 60.0 mol %. On the basis of the data from the polyphasic characterization, strain Y2 T represents a novel species, for which the name Altererythrobacter oceanensis sp. nov. is proposed. The type strain is Y2 T (=CGMCC 1.12752 T =LMG 28109 T). 相似文献
12.
A new bacterial strain, designated as FF42 T, was isolated from feces of domestic pigs—collected from Suwon, Korea—and was characterized to determine its taxonomic position. Strain FF42 T was observed to be Gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. Based on the phylogenetic and 16S rRNA sequence analyses, it was revealed that strain FF42 T belonged to the genus Comamonas. The highest degree of sequence similarities was determined to be with Comamonas zonglianii BF-3 T (96.3 %), Comamonas composti CC-YY287 T (96.1 %), and Comamonas nitrativorans 23310 T (95.9 %), while showing less than 95.6 % identity with the remaining Comamonas species. Growth of strain FF42 T occurred between 25 and 40 °C (optimum, 28 °C) and at pH of 5-9 (optimum, pH 6.0). It grew in the presence of 0–3 % (w/v) NaCl while minimally tolerating at 3 % (w/v) NaCl. Biochemical and physiological tests revealed phenotypic differentiation of strain FF42 T to other members of the genus Comamonas. The predominant quinone is ubiquinone (Q-8). The major cellular fatty acids were C 10:0 3OH, C 16:0, summed feature 3 (C 16:1 ω7 c/C 16:1 ω6 c), and summed feature 8 (C 18:1 ω6 c/C 18:1 ω7 c), all of which have previously been reported to occur in the species of the genus Comamonas. The G+C molar content for strain FF42 T is 60.2 mol %. Based on phylogenetic and phenotypic analyses, strain FF42 T (=KEMC 1002-058 T=JCM 17561 T) is clearly referred to be a novel species for the genus Comamonas, for which the name Comamonas faecalis sp. nov. is proposed. 相似文献
13.
A Gram-stain negative, motile, rod-shaped bacterium, designated strain WM-2 T, was isolated from a forest soil in Sihui City, South China, and characterized by means of a polyphasic approach. Growth occurred with 0–5 % (w/v) NaCl (optimum 0–1 %) and at pH 5.0–10.5 (optimum pH 8.5) and 4–40 °C (optimum 30 °C) in Luria–Bertani medium. Comparative 16S rRNA gene sequence analyses showed that strain WM-2 T is a member of the genus Pseudomonas and most closely related to P. guguanensis, P. oleovorans subsp. lubricantis, P. toyotomiensis, P. alcaliphila and P. mendocina with 97.1–96.6 % sequence similarities. In terms of gyrB and rpoB gene sequences, strain WM-2 T showed the highest similarity with the type strains of the species P. toyotomiensis and P. alcaliphila. The DNA–DNA relatedness values of strain WM-2 T with P. guguanensis and P. oleovorans subsp. lubricantis was 48.7 and 37.2 %, respectively. Chemotaxonomic characteristics (the main ubiquinone Q-9, major fatty acids C 18:1 ω7 c/C 18:1 ω6 c, C 16:0 and C 16:1 ω7 c/C 16:1 ω6 c and DNA G+C content 65.2 ± 0.7 mol%) were similar to those of members of the genus Pseudomonas. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown aminophospholipid, an unknown phospholipid and five unknown lipids. According to the results of polyphasic analyses, strain WM-2 T represents a novel species in the genus Pseudomonas, for which the name Pseudomonas sihuiensis sp. nov. is proposed. The type strain is WM-2 T (=KCTC 32246 T=CGMCC 1.12407 T). 相似文献
14.
A Gram-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain designated KHI67 T was isolated from sediment of the Gapcheon River in South Korea and its taxonomic position was investigated by using a polyphasic approach. Strain KHI67 T was observed to grow optimally at 25–30 °C and at pH 7.0 on nutrient and R2A agar. On the basis of 16S rRNA gene sequence similarity, strain KHI67 T was shown to belong to the family Sphingomonadaceae and was related to Sphingomonas faeni MA-olki T (97.6 % sequence similarity), Sphingomonas aerolata NW12 T (97.5 %) and Sphingomonas aurantiaca MA101b T (97.3 %). The G + C content of the genomic DNA was determined to be 65.6 %. The major ubiquinone was found to be Q-10, the major polyamine was identified as homospermidine and the major fatty acids identified were summed feature 8 (comprising C 18:1 ω7 c/ ω6 c; 37.0 %), C 16:0 (13.0 %), summed feature 3 (comprising C 16:1 ω7 c/C 16:1 ω6 c; 12.8 %) and C 14:0 2OH (9.3 %). DNA and chemotaxonomic data supported the affiliation of strain KHI67 T to the genus Sphingomonas. The DNA–DNA relatedness values between strain KHI67 T and its closest phylogenetic neighbours were below 15 %. Strain KHI67 T could be differentiated genotypically and phenotypically from the recognised species of the genus Sphingomonas. The isolate therefore represents a novel species, for which the name Sphingomonas ginsenosidivorax sp. nov. is proposed, with the type strain KHI67 T (=KACC 14951 T = JCM 17076 T = LMG 25801 T). 相似文献
15.
A taxonomic study was carried out on strain 22II-S10r2 T, which was isolated from the deep sea sediment of the Atlantic Ocean using oil-degrading enrichment. The bacterium was Gram-negative, oxidase positive and catalase negative, spherical in shape, and motile by polar flagella. Growth was observed at salinities of 0.5–7 % and at temperatures of 10–41 °C. The isolate was capable of aesculin hydrolysis, but unable to reduce nitrate to nitrite or degrade Tween 80 or gelatine. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10r2 T belonged to the family Ectothiorhodospiraceae, with highest sequence similarity to Thioalkalivibrio sulfidiphilus HL-EbGR7 T (90.9 % similarity). The principal fatty acids were Sum In Feature 8 (C 18:1 ω7 c/ ω6 c (29.9 %), C 18:1 ω9 c (13.5 %), C 16:1 ω5 c (12.3 %), C 12:03OH (6.8 %), C 18:1 ω5 c (5.7 %) and C 16:0 (5.3 %). The G+C content of the chromosomal DNA was 60.7 mol%. The respiratory quinone was determined to be Q-7 (25 %) and Q-8 (75 %). Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, glycolipid, three phospholipids and lipid were present. The strain was aerobic, non-phototrophic and non-chemolithoautotrophic. The combined genotypic and phenotypic data show that strain 22II-S10r2 T represents a novel species within a novel genus, for which the name Maricoccus atlantica gen. nov. sp. nov. is proposed, with the type strain 22II-S10r2 T (=CGMCC NO.1.12317 T = LMG 27155 T = MCCC 1A09384 T). 相似文献
16.
A Gram-negative, non-spore-forming, aerobic, motile and rod-shaped or ovoid bacterial strain, designated MA1-3 T, was isolated from a sea squirt ( Halocynthia roretzi) collected from the South sea in South Korea. Strain MA1-3 T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain MA1-3 T fell within the clade comprising Colwellia species, clustering coherently with the type strains of Colwellia aestuarii, Colwellia polaris and Colwellia chukchiensis, showing sequence similarity values of 97.2, 96.4 and 95.6 %, respectively. It exhibited 16S rRNA gene sequence similarity values of 93.9–96.1 % to the type strains of the other Colwellia species. Strain MA1-3 T was found to contain Q-8 as the predominant ubiquinone and C 16:1 ω7 c and/or C 16:1 ω6 c, C 16:0 and C 16:1 ω9 c as the major fatty acids. The DNA G+C content of strain MA1-3 T was determined to be 39.1 mol% and its mean DNA–DNA relatedness value with the type strain of C. aestuarii was 13 ± 5.4 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separated from other Colwellia species. On the basis of the data presented, strain MA1-3 T is considered to represent a novel species of the genus Colwellia, for which the name Colwellia meonggei sp. nov. is proposed. The type strain is MA1-3 T (=KCTC 32380 T = CECT 8302 T). 相似文献
17.
A Gram-negative, aerobic, non-motile, dark brown-coloured and rod-shaped bacterial strain, designated G-MB1 T, was isolated from a tidal flat sediment of the South Sea, South Korea. Strain G-MB1 T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain G-MB1 T fell within the clade comprising Thalassomonas species, clustering with the type strains of Thalassomonas agarivorans, Thalassomonas loyana, Thalassomonas ganghwensis and Thalassomonas agariperforans, with which it exhibited 16S rRNA gene sequence similarity values of 96.0–96.9 %. The 16S rRNA gene sequence similarity values between strain G-MB1 T and the type strains of the other Thalassomonas species were 94.6–95.1 %. Strain G-MB1 T was found to contain Q-8 as the predominant ubiquinone and C 16:0, C 17:1 ω8 c, C 16:1 ω9 c, C 12:0 3-OH and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c) as the major fatty acids. The major polar lipids of strain G-MB1 T were phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The DNA G+C content of strain G-MB1 T was determined to be 42.4 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain G-MB1 T is separated from other Thalassomonas species. On the basis of the data presented, strain G-MB1 T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas fusca sp. nov. is proposed. The type strain is G-MB1 T (=KCTC 32499 T = NBRC 109830 T). 相似文献
18.
A taxonomic study was carried out on strain D104 T, which was isolated from deep-sea subsurface sediment sample from the Arctic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase weakly positive, rod shaped, motile by means of polar flagellum. The organism grows between 4 and 37 °C (optimum 25–28 °C) and 0.5–6 % NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D104 T belongs to the genus Marinomonas, with highest sequence similarities of 97.7 % to Marinomonas ushuaiensis DSM 15871 T, followed by M. dokdonensis DSW10-10 T (96.9 %), M. arenicola KMM 3893 T (96.7 %), M. arctica 328 T (96.6 %) and other 18 species of the genus Marinomonas (94.4–96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain D104 T and M. ushuaiensis DSM 15871 T were 84.24 % and 20.80 ± 2.33 % respectively. The principal fatty acids were C 16:0, sum in feature 3 (C 16:1 ω7 c/C 16:1 ω6 c), sum in feature 8 (C 18:1 ω7 c/C 18:1 ω6 c) and C 12:1 3OH. The G + C content of the chromosomal DNA was determined to be 44.8 mol%. The respiratory quinone was found to be Q8 (100 %). Polar lipids include phosphatidylglycerol and phosphatidylethanolamine as major phospholipids and aminolipid and phospholipid as minor components. The results of the genotypic and phenotypic analyses indicate that strain D104 T represents a novel species of the genus Marinomonas, for which the name Marinomonas profundimaris sp. nov. is proposed, with the type strain D104 T (=MCCC 1A07573 T = LMG 27696 T). 相似文献
19.
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3 T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3 T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701 T and P. sagittaria JCM 18195 T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA–DNA hybridization between strain WM-3 T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C 16:0 (30.0 %), C 16:1 ω6c and/or C 16:1 ω7c (19.3 %) and C 18:1 ω6 c and/or C 18:1 ω7 c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3 T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3 T (=KCTC 32247 T =CGMCC 1.12417 T). 相似文献
20.
Two novel species of the genus Deinococcus, designated SYSU M49105T and SYSU M42101T, were isolated from freshwater samples of the Pearl River estuary in Guangdong, China. Phylogenetic analysis using 16S rRNA gene sequence indicated that strains SYSU M49105T and SYSU M42101T showed the highest sequence similarities to Deinococcus aetherius JCM 11751 T (93.6%) and Deinococcus multiflagellatus NBRC 112888 T (97.3%), respectively. Cells of both strains were Gram-staining positive, aerobic, coccus-shaped, oxidase-negative and non-motile. The cell wall contained meso-diaminopimelic acid as their diagnostic diamino acid. MK-8 was the predominant respiratory quinone for both strains. The polar lipid profile of SYSU M49105T contained two unidentified phosphoglycolipids, nine unidentified glycolipids, and five unidentified polar lipids. SYSU M42101T had one unidentified phosphoglycolipid, nine unidentified glycolipids, one unidentified aminophospholipid and four unidentified polar lipids. The major fatty acids of strains SYSU M49105T and SYSU M42101T were summed feature 3 (C16:1 ω7c and/ or C16:1 ω6c) and C16:0. The G?+?C contents of the novel isolates based on genomic DNAs were 69.6% and 67.4%, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU M49105T and SYSU M42101T should be considered to represent two novel species in the genus Deinococcus, for which the names Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov. were proposed with the type strains SYSU M49105T (=?KCTC 43258 T?=?CGMCC 1.18609 T) and SYSU M42101T (=?KCTC 43257 T?=?CGMCC 1.18614 T), respectively. 相似文献
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