Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens

Background  

Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals. However, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. There are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pesticide-free food. Thus, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative to chemical pesticides for more rational and safe crop management.     

Transcriptome profiling of a curdlan-producing <Emphasis Type="Italic">Agrobacterium</Emphasis> reveals conserved regulatory mechanisms of exopolysaccharide biosynthesis

Background  

The ability to synthesize exopolysaccharides (EPS) is widespread among microorganisms, and microbial EPS play important roles in biofilm formation, pathogen persistence, and applications in the food and medical industries. Although it is well established that EPS synthesis is invariably in response to environmental cues, it remains largely unknown how various environmental signals trigger activation of the biochemical synthesis machinery.     

Genetic diversity and relationships in mulberry (genusMorus) as revealed by RAPD and ISSR marker assays

Background  

The genus Morus, known as mulberry, is a dioecious and cross-pollinating plant that is the sole food for the domesticated silkworm, Bombyx mori. Traditional methods using morphological traits for classification are largely unsuccessful in establishing the diversity and relationships among different mulberry species because of environmental influence on traits of interest. As a more robust alternative, PCR based marker assays including RAPD and ISSR were employed to study the genetic diversity and interrelationships among twelve domesticated and three wild mulberry species.     

The sustainability of communicative packaging concepts in the food supply chain. A case study: part 1. Life cycle assessment

Purpose  

In recent years, a new perspective for food packaging has emerged as a result of several issues like quality, safety, competitive prices or providing of useful information to consumers. This new perspective is called communicative packaging. Communicative packaging may influence consumers/companies on purchasing decisions. Since the environmental evaluation of such systems has not yet been performed, this paper is focused on the environmental evaluation of a flexible best-before-date (FBBD) communicative device on a packaging consumer unit and its implications on reducing environmental impacts related to fresh products. This consumer unit consists of a nanoclay-based polylactic acid tray filled with pork chops.     

Environmental impacts of German food consumption and food losses

Purpose

The objective was to assess the environmental burden of food consumption and food losses in Germany with the aim to define measures to reduce environmentally relevant food losses. To support the finding of measurements, the study provides differentiated information on life phases (agriculture, processing, retailer, and consumption), consumption places (in-house and out-of-home), and the average German food basket consisting of eight food categories.

Methods

In order to obtain information on the environmental impacts of German food consumption, the study analyzed the material flows of the food products in the German food basket starting from consumption phase and going backwards until agricultural production. The analysis includes all relevant impact categories such as GWP, freshwater and marine eutrophication, particular matter formation, and agricultural land and water use. The life stages consumers, retail, wholesale, food production, and agriculture have been taken into account. Furthermore, transports to and within Germany have been considered. Consumption and production data have been taken from the German income and consumption sample, German production and trade statistics, and studies recently carried out on food losses. In order to model German food consumption, some simplifications had to be done.

Results and discussion

Results show that German food consumption is responsible for 2.7 t of greenhouse gases per person and year. Fourteen cubic meters of blue water is used for agricultural food production per person, and 2673 m² of agricultural land is occupied each year per German for food consumption. Between 14 and 20 % of the environmental burdens (depending on the impact category) result from food losses along the value chain. Out-of-home consumption is responsible for 8 to 28 % of the total environmental impacts (depending on the impact category). In particular, animal products cause high environmental burdens. Regarding life cycle phases, agriculture and consumption cause the highest impacts: together, they are responsible for more than 87 % of the total environmental burdens.

Conclusions

The study shows that food production and consumption as well as food losses along the value chain are of high relevance regarding Germany’s environmental impacts. In particular, animal products are responsible for high environmental burdens. Thus, with respect to reducing environmentally relevant food losses, measures should focus in particular on the reduction of food waste of animal origin. The most relevant life cycle phases to reduce environmental impacts are agricultural production and consumption in households and out-of-home.

     

MEXALCA: a modular method for the extrapolation of crop LCA

Purpose  

Life cycle assessment (LCA) is widely used for the environmental assessment of food products, but difficulties arise when evaluating large portfolios of food products or when faced with a diversity of sources of ingredients and/or frequent changes of suppliers. In such situations, a specific, in-depth assessment of each ingredient is not feasible, and screening approaches using a few LCIA (Life Cycle Impact Assessment) results are not recommended. The goal of this paper is to propose an intermediate solution between a screening assessment using limited data and specific LCA for all products considering all sources of ingredients.     

Are public payments for organic farming cost-effective? Combining a decision-support model with LCA

Purpose  

The agricultural sector fulfils several functions such as the production of food energy and landscape conservation. An equilibrium between economic development and environmental protection should be found and research should aid political decision-making. In recent years, great efforts have been made to assess the environmental and economic implications of changes in both environmental and agricultural policies. Life cycle assessment (LCA) has been extended by cost functions and social parameters. The validation of suggestions for political measures can be improved by combining existing environmental and economic models. This approach is applied in this paper in order to compare an increased support for organic farming with specific environmental policy measures, focusing on the resulting impacts and socioeconomic indicators.     

City Level of Income and Urbanization and Availability of Food Stores and Food Service Places in China

Objective

The contribution of unhealthy dietary patterns to the epidemic of obesity has been well recognized. Differences in availability of foods may have an important influence on individual eating behaviors and health disparities. This study examined the availability of food stores and food service places by city characteristics on city level of income and urbanization.

Methods

The cross-sectional survey was comprised of two parts: (1) an on-site observation to measure availability of food stores and food service places in 12 cities of China; (2) an in-store survey to determine the presence of fresh/frozen vegetables or fruits in all food stores. Trained investigators walked all the streets/roads within study tracts to identify all the food outlets. An observational survey questionnaire was used in all food stores to determine the presence of fresh/frozen vegetables or fruits. Urbanization index was determined for each city using a principal components factor analysis. City level of income and urbanization and numbers of each type of food stores and food service places were examined using negative binomial regression models.

Results

Large-sized supermarkets and specialty retailers had higher number of fresh/frozen vegetables or fruits sold compared to small/medium-sized markets. High-income versus low-income, high urbanized versus low urbanized areas had significantly more large-sized supermarkets and fewer small/medium-sized markets. In terms of restaurants, high urbanized cities had more western fast food restaurants and no statistically significant difference in the relative availability of any type of restaurants was found between high- and low-income areas.

Conclusions

The findings suggested food environment disparities did exist in different cities of China.     

Multi-attribute life cycle assessment of preventive maintenance treatments on road pavements for achieving environmental sustainability

Purpose  

Although a significant number of environmental protection measures concerning industrial products and processes have emerged over the past few years, similar measures have only started to appear in road construction and related practices. There is a need for understanding what a “sustainable pavement” would entail in terms of greenhouse gas emissions and energy consumption. Since environmental impact assessment of major projects is becoming mandatory in many countries, various research projects attempt to evaluate the environmental impact of different pavement materials, technologies, or processes over the road life cycle. To support these efforts, there is a need to measure and describe different aspects of sustainability related to road pavements. In particular, keeping road pavements at high service levels through a preventive maintenance approach during the pavement service life has been proven to provide significant improvement of their performance and reduce their deterioration rate.     

Quantitative genetic analysis of life-history traits of <Emphasis Type="Italic">Caenorhabditis elegans</Emphasis> in stressful environments

Background  

Organisms live in environments that vary. For life-history traits that vary across environments, fitness will be maximised when the phenotype is appropriately matched to the environmental conditions. For the free-living nematode Caenorhabditis elegans, we have investigated how two major life-history traits, (i) the development of environmentally resistant dauer larvae and (ii) reproduction, respond to environmental stress (high population density and low food availability), and how these traits vary between lines and the genetic basis of this variation.     

Estimating the greenhouse gas footprint of Knorr

Purpose  

Greenhouse gas (GHG) emissions have been identified as one of Unilever’s priority environmental impact themes: this assessment was therefore conducted to help the Knorr brand measure and understand the GHG emissions related to its product portfolio, identify opportunities to manage GHG emissions in the Unilever-owned operations (manufacture) and influence managed reductions elsewhere in the Knorr product lifecycles, and assess the impact of the brand’s innovation and portfolio strategies on its GHG footprint.     

CcpA affects expression of the groESL and dnaK operons in Lactobacillus plantarum

Background  

Lactic acid bacteria (LAB) are widely used in food industry and their growth performance is important for the quality of the fermented product. During industrial processes changes in temperature may represent an environmental stress to be overcome by starters and non-starters LAB. Studies on adaptation to heat shock have shown the involvement of the chaperon system-proteins in various Gram-positive bacteria. The corresponding operons, namely the dnaK and groESL operons, are controlled by a negative mechanism involving the HrcA repressor protein binding to the cis acting element CIRCE.     

LCA study of unconsumed food and the influence of consumer behavior

Purpose

In the light of anthropogenic resource depletion and the resulting influences on the greenhouse effect as well as globally occurring famine, food waste has garnered increased public interest in recent years. The aim of this study is to analyze the environmental impacts of food waste and to determine to what extent consumers’ behavior influences the environmental burden of food consumption in households.

Methods

A life cycle assessment (LCA) study of three food products is conducted, following the ISO 14040/44 life cycle assessment guidelines. This study addresses the impact categories climate change (GWP100), eutrophication (EP), and acidification (AP). Primary energy demand (PED) is also calculated. For adequate representation of consumer behavior, scenarios based on various consumer types are generated in the customer stage. The customer stage includes the food-related activities: shopping, storage, preparation, and disposal of food products as well as the disposal of the sales packaging.

Results and discussion

If the consumer acts careless towards the environment, the customer stage appears as the main hotspot in the LCA of food products. The environmental impact of food products can be reduced in the customer stage by an environmentally conscious consumer. Shopping has the highest effect on the evaluated impact categories and the PED. Additionally, consumers can reduce the resulting emissions by decreasing the electric energy demand, particularly concerning food storage or preparation. Moreover, results show that the avoidance of wasting unconsumed food can reduce the environmental impact significantly.

Conclusions

Results of this study show that the influence of consumer behavior on the LCA results is important. The customer stage of food products should not be overlooked in LCA studies. To enable comparison among results of other LCA studies, the LCA community needs to develop a common methodology for modeling consumer behavior.

     

Life cycle assessment of the environmental influence of wooden and concrete utility poles based on service lifetime

Purpose

Many applications of life cycle assessment do not consider the variability of the service lifetime of different structures, and this may be a relevant factor in an environmental impact assessment. This paper aims to determine the influence of the service lifetime on the potential environmental impacts of wooden and concrete poles in the electricity distribution system.

Methods

The estimation of service lifetime was conducted using the factorial method. The life cycle assessment was applied using SimaPro software and considered the entire life cycle of utility poles, from the extraction of raw materials to the final disposal. Then, an evaluation of the environmental impacts using the CML IA baseline method was performed. The study included the analysis of uncertainty using the Monte Carlo method.

Results and discussion

In general, the wooden poles had a lower potential environmental impact compared to the concrete poles. The result of the sensitivity analysis considering the variability of the chromated copper arsenate wood preservative retention rate suggests that the frequency of maintenance affects the service lifetime. Often, the comparison of products in the LCA perspective is carried out by considering similar useful lifetime services for the different alternatives, and this study shows that the environmental performance of products or services is directly proportional to the lifetime. It is a crucial parameter that has to be clarified in order to reduce uncertainty in the results.

Conclusions

Thus, some factors such as material quality, design adjustments and routine maintenance extend the service lifetime of a product or process and are shown to be effective ways to reduce environmental impacts. Therefore, the service lifetime has a significant influence on the development of the life cycle assessment. Comparative LCA studies are often sensitive to parameters that may even change the ranking of selected impact categories. All in all, from the sensitivity analysis highlighted in this study, the variability of lifetime service has proven to be one of the most prominent factors influencing comparative LCA results.

     

Epigenetic effects on the mouse mandible: common features and discrepancies in remodeling due to muscular dystrophy and response to food consistency

Background  

In wild populations phenotypic differentiation of skeletal structures is influenced by many factors including epigenetic interactions and plastic response to environmental influences, possibly blurring the expression of genetic differences. In contrast, laboratory animals provide the opportunity to separate environmental from genetic effects. The mouse mandible is particularly prone to such plastic variations because bone remodeling occurs late in postnatal ontogeny, in interaction with muscular loading. In order to understand the impact of this process on mandible morphology, we investigated how change in the masticatory function affects the mandible shape, and its pattern of variation. Breeding laboratory mice on food of different consistencies mimicked a natural variation in feeding ecology, whereas mice affected by the murine analogue of the Duchenne muscular dystrophy provided a case of pathological modification of the mastication process.     

Analysis of raw cork production in Portugal and Catalonia using life cycle assessment

Purpose

This study aims to (1) evaluate the environmental impacts associated with the three types of raw cork produced in Portuguese cork oak woodlands (in Alentejo region) considering two alternative practices for stand establishment (plantation and natural regeneration), (2) compare the environmental impacts of raw cork production in Portuguese cork oak woodlands and in Catalonian cork oak forests, and (3) assess the influence of different allocation criteria for partitioning the environmental impacts between the different types of raw cork produced.

Methods

A cradle-to-gate approach was adopted starting with stand establishment up to cork storage in a field yard. The system boundaries include all management operations undertaken during the following stages: stand establishment, stand tending, cork stripping, and field recovery. The allocation of the environmental impacts to reproduction, second, and virgin cork was based on mass and market price criteria. An alternative allocation approach was simulated by allocating environmental impacts also to the wood produced in the cork oak stands. The impact assessment was performed using the characterization factors recommended by the International Reference Life Cycle Data System (ILCD).

Results and discussion

In Portugal, cork produced from naturally regenerated stands has a better environmental performance than cork produced from planted stands, but the differences are smaller than 10 %. Different management models of cork oak stands in Portugal and Catalonia (agro-silvopastoral system and forest system, respectively) originate different impact levels, which tend to be significantly lower in Catalonia. The environmental hot spots in the two regions are also distinct. In Catalonia, they are associated with cleaning, road maintenance, and worker and cork transport. In Portugal, they are fertilization, pruning, and cleaning. The two allocation criteria affect significantly the results obtained for virgin cork in Portugal and for virgin and second cork in Catalonia. Besides, when impacts are also allocated to wood, mass allocation should be avoided as it would not create incentives for a sustainable management of cork oak stands.

Conclusions

The environmental impact from Catalonian cork may be reduced by decreasing mechanized shrub cleaning and road maintenance operations through the introduction of livestock in cork oak forests, and also by a better planning of management operations. For the Portuguese cork, improvements may be achieved by optimizing fertilizer dosage, planting nitrogen-fixing crops and pastures that improve soil quality, avoiding unnecessary operations, improving the efficiency of management operations, and increasing tree density.     

SENSE tool: easy-to-use web-based tool to calculate food product environmental impact

Purpose

The purpose of the European SENSE project was to define an integral system to assess and communicate the environmental impacts of food products and to develop a web-based tool for Small and Medium size Enterprises (SMEs). The tool has been tested in salmon, beef-and-dairy, and fruit juice production sectors.

Methods

The SENSE project has evaluated several existing methodologies for environmental impact assessment over the life cycle including also social aspects, in order to deliver a new integral system for the environmental and social assessment of agricultural and aquaculture food products.

Results and discussion

The system includes a standardization of a data gathering system, a selection of relevant key environmental performance indicators for food supply chains and a common methodology to perform simplified life cycle impact assessment. The results are based on collected information on the use of resources and emissions generated along the supply chain of food or drink products. The main result is a web-based software tool that is based on a summation of the partial impacts of the different steps in food supply chains. In this software, different actors in the supply chain can enter their own data and link them to the data of other companies. The results obtained in the tool could be used for at least six different approaches: (i) environmental impact assessment of the product, (ii) food chain hot spot identification, (iii) comparison of hypothetical or real improvement scenarios, (iv) assessment of the environmental impact development over the years, (v) benchmarking opportunity for the companies, and (vi) a business to business communication strategy. The scientific robustness of the tool has been tested comparing the obtained results with the same analysis with commercial software.

Conclusions

The SENSE tool is a simplified tool designed for food and drink SMEs to assess their sustainability on their own. This cannot be fully compared to a complete LCA study. The testing with SMEs showed that they need additional support for filling in the questionnaires correctly and interpret the results. The simplified evaluation of environmental impacts based on a life cycle approach could lead to benefits to SMEs within the food industry. The future application and development of the tool will be focused on adapting the tool to the Product Environmental Footprint initiative requirements and self-assessment opportunities.

     

Modelling,assessing, and ranking public procurement options for a climate-friendly catering service

Purpose

Several scientific papers and technical reports have discussed the role of green public procurement in the food sector. Different strategies for the restoration sector have been identified. However, there is not yet a common understanding of which policies could be the most efficient in reducing the global warming potential of the public restoration service. This paper assesses a set of procurement policies, ranking them according to their potential to reduce the greenhouse gas emissions of public catering.

Methods

Eleven relevant green public procurement policies were identified from the literature. These are discussed in the context of a case study of the school catering service in the city of Turin (Italy). Initially, a life cycle approach is applied to a baseline scenario of the collective restoration system of the city, to quantify the quantity of greenhouse gases produced by the entire catering service (including all stages from the production of food to the management of waste from kitchens and canteens). Afterwards, the 11 policies were applied to the baseline scenario so that the potential improvement achieved by each policy could be quantified.

Results and discussion

The baseline scenario resulted in 1.67 kgCO₂eq per average meal. The production of food dominates the global warming potential of the full service, being responsible for about 78% of the greenhouse gas emissions. Among the selected policies, a change in diet was the most effective (leading to a 32% reduction of the CO₂eq emissions), followed by the adoption of improved food production practices (11% reduction) and the purchasing of certified green electricity (6% reduction).

Conclusions

The proposed method allows the assessment of procurement policies in the catering service by applying a simplified life cycle approach that considers all the stages of the process. Public authorities and other stakeholders could benefit from basing their decisions upon scientific evidence and avoiding the prioritisation of policies based on personal opinions or weak evidence. Uncertainties and areas for improvement in the method have been also identified for future investigation.