共查询到20条相似文献,搜索用时 406 毫秒
1.
《Molecular & cellular proteomics : MCP》2020,19(7):1120-1131
Highlights
- •Quantitative proteomics of isolated lysosomes, autophagosomes and proteasomes.
- •Pharmacological inhibition of proteasomes leads to their accumulation within lysosomes.
- •Inhibition of classical autophagy pathways cannot completely block this process.
- •Known autophagy adaptor proteins are not involved.
2.
《Molecular & cellular proteomics : MCP》2020,19(11):1896-1909
Highlights
- •Epitope-tagging of a proteasome subunit allows for facile immuno-isolation.
- •An engineered yeast strain permits capture of proteasome-associated substrates.
- •MS/MS identified all 33 resident proteasome subunits in the 20S and 19S particles.
- •Analysis of associated proteins and characterization of newly identified ERAD substrate.
3.
《Molecular & cellular proteomics : MCP》2020,19(11):1876-1895
Highlights
- •Guidelines for studying protein complexes via co-fractionation mass spectrometry.
- •A novel procedure for profiling gold standard protein complexes in CF-MS data.
- •Recommendations for efficient CF-MS fractionation collection.
- •Scoring metric recommendations for precise and sensitive CF-MS data analysis.
4.
Fengchao Yu Sarah E. Haynes Guo Ci Teo Dmitry M. Avtonomov Daniel A. Polasky Alexey I. Nesvizhskii 《Molecular & cellular proteomics : MCP》2020,19(9):1575-1585
Highlights
- •MSFragger now supports raw timsTOF PASEF data.
- •IonQuant performs fast and accurate feature detection and quantification.
- •MSFragger and IonQuant provide excellent performance for timsTOF PASEF data.
- •Flexibility allows for complex analyses, such as semi-enzymatic and open search.
5.
《Molecular & cellular proteomics : MCP》2020,19(4):655-671
Highlights
- •Acute inhibition of growth-regulatory AGC-kinases Sch9, PKA and Ypk1.
- •Phospho-proteomic profiling of 6373 phsopho-sites.
- •Ypk1 regulates phospho-sites in RRxS/T-context and functionally overlaps with PKA.
- •Ypk1 and PKA activate trehalase activity of Nth1.
6.
《Molecular & cellular proteomics : MCP》2020,19(2):326-343
Highlights
- •NCMR is crucial for substrate recognition and activity regulation.
- •MASTL conserves a cryptic C-Lobe in the non-conserved middle region.
- •MASTL450 containing the cryptic C-lobe is observed in cancer cell lines.
- •Key phosphorylation sites for MASTL provide an activation model.
7.
《Molecular & cellular proteomics : MCP》2020,19(4):624-639
Highlights
- •Used affinity-enrichable, isotopically coded, and MS-cleavable crosslinker.
- •Targeted acquisition strategy based on isotopic-coding described and evaluated.
- •Novel data analysis pipeline developed provides improved crosslink identification.
- •Large dataset reveals hundreds of mitochondrial protein-protein interactions.
8.
Sung-Soo Park Daniela Ponce-Balbuena Rork Kuick Guadalupe Guerrero-Serna Justin Yoon Dattatreya Mellacheruvu Kevin P. Conlon Venkatesha Basrur Alexey I. Nesvizhskii Jos Jalife Jean-Franois Rual 《Molecular & cellular proteomics : MCP》2020,19(9):1436-1449
Highlights
- •Generation using BioID of a map of the Kir2.1 interactome with 218 interactions.
- •Identification of Kir2.1WT- versus Kir2.1Δ314-315-preferred interactors.
- •Identification of the desmosome protein PKP4 as a new modulator of IKir2.1 currents.
9.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
Highlights
- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
10.
《Molecular & cellular proteomics : MCP》2020,19(6):971-993
Highlights
- •db/db β-cells restores appropriate insulin stores and normalize secretory function.
- •Numerous changes in the phosphorylation and sialylation states by euglycemic rest.
- •Restoration of numerous dysfunctional biological processes following euglycemic rest.
- •β-cell adaptive flexibility may lead to improvement in endogenous β-cell function.
11.
《Molecular & cellular proteomics : MCP》2020,19(3):467-477
Highlights
- •Each component of the AMPK trimeric complex was profiled by interaction proteomics.
- •The subunit composition of the AMPK complex directs interactions to distinct proteins.
- •AMPK interacts with Artemis and plays a role in Non-Homologous End Joining.
12.
Proteomics of Galápagos Marine Iguanas Links Function of Femoral Gland Proteins to the Immune System
Frederik Tellkamp Franziska Lang Alejandro Ibez Lena Abraham Galo Quezada Stefan Günther Mario Looso Fabian Jannik Tann Daniela Müller Franz Cemic Jürgen Hemberger Sebastian Steinfartz Marcus Krüger 《Molecular & cellular proteomics : MCP》2020,19(9):1523-1532
13.
《Molecular & cellular proteomics : MCP》2020,19(3):490-500
Highlights
- •HuProt array-based identification of autoantigens in serum of early lung cancer.
- •Independent validation of early lung cancer biomarker candidates with ELISA.
- •Bioinformatics-aided identification of a biomarker panel.
- •Independent verification of the panel with ELISA and immunohistochemistry.
14.
Barbara Steigenberger Henk W.P. van den Toorn Emiel Bijl Jean-Franois Greisch Oliver Rther Markus Lubeck Roland J. Pieters Albert J.R. Heck Richard A. Scheltema 《Molecular & cellular proteomics : MCP》2020,19(10):1677-1687
Highlights
- •Cross-linked peptides are physically separated from mono-linked peptides in the gas-phase by TIMS ion mobility.
- •Development of a novel data acquisition routine that a-priori distinguishes cross-linked from mono-linked peptides called caps-PASEF.
- •First application of PhoX-driven cross-linking mass spectrometry on the timsTOF Pro.
- •Application of cross-linking mass spectrometry to medium to high complexity samples.
15.
《Molecular & cellular proteomics : MCP》2020,19(11):1826-1849
Highlights
- •Identification of subcellular protein interactomes via proximity labeling and quantitative multiplexed proteomics.
- •SEC61β and RPN1 interactomes overlap with translocon-associated protein networks.
- •SEC62 interacts with redox-linked proteins and ER luminal chaperones.
- •LRRC59 directly interacts with mRNA translation factors and SRP machinery on the ER.
16.
17.
《Molecular & cellular proteomics : MCP》2020,19(7):1088-1103
Highlights
- •Rapid DIA-only library building with gas-phase fractionation.
- •Recommended DIA acquisition strategies with staggered windows and forbidden zones.
- •Optimized DIA instrument settings for several Thermo Orbitrap instruments.
- •Data analysis tutorial using open source DIA software.
18.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
Highlights
- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
19.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
Highlights
- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
20.
《Molecular & cellular proteomics : MCP》2020,19(1):50-64
Highlights
- •BioID reveals the proximity partners of RSK family members.
- •All RSK isoforms associate with and phosphorylate p120ctn on Ser320.
- •RSK negatively regulates adherens junctions and reduces cell-cell adhesion.
- •p120ctn phosphorylation plays a role in the reorganization of proximity partners.