Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles

We show that using low denaturation temperatures (80-88 degrees C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the PCR melting profile (PCR MP). A single pattern obtained for a given temperature and a set of patterns arising after application of several denaturation temperatures (PCR MP) are very specific for the given bacterial genome and may be used for strain characterisation and differentiation. The method may also be used for amplification and isolation of the less stable DNA fragments in a genome.     

Immunological effectiveness of secondary revaccination against tetanus performed at prolonged intervals

After primary immunization with adsorbed DPT vaccine antitetanus immunity was retained for 9-10 years in 90.6-98.4% of children covered by this study. The second booster immunization of children against tetanus with adsorbed DT toxoid with reduced antigen content, made at an interval prolonged to 7-10 years under the conditions of lower antitoxic immunity, ensured a high level of intense antitetanus immunity. These data point to the possibility of prolonging the interval between booster immunizations to 9-10 years and reducing the number of injections and antigenic load.     

Jejunal crypt stem-cell survival after fractionated gamma-irradiation performed at different dose rates

Jejunal crypt survival after fractionated total body irradiation of C3H mice given at dose rates between 1.2 and 0.08 Gy/min was studied and the results analysed according to the linear quadratic model. Whereas alpha was independent of dose rate beta decreased with dose rate to approach zero at about 0.01 Gy/min. During the period of recovery, sublethal damage from doses given at high dose rate interact with low dose rate irradiation given immediately after, and increases its effectiveness.     

Motor unit firing rates during isometric voluntary contractions performed at different muscle lengths

Firing rates of motor units and surface EMG were measured from the triceps brachii muscles of able-bodied subjects during brief submaximal and maximal isometric voluntary contractions made at 5 elbow joint angles that covered the entire physiological range of muscle lengths. Muscle activation at the longest, midlength, and shortest muscle lengths, measured by twitch occlusion, averaged 98%, 97%, and 93% respectively, with each subject able to achieve complete activation during some contractions. As expected, the strongest contractions were recorded at 90 degrees of elbow flexion. Mean motor unit firing rates and surface EMG increased with contraction intensity at each muscle length. For any given absolute contraction intensity, motor unit firing rates varied when muscle length was changed. However, mean motor unit firing rates were independent of muscle length when contractions were compared with the intensity of the maximal voluntary contraction (MVC) achieved at each joint angle.     

Ligation mediated PCR performed at low denaturation temperatures—PCR melting profiles

We show that using low denaturation temperatures (80–88°C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the PCR melting profile (PCR MP). A single pattern obtained for a given temperature and a set of patterns arising after application of several denaturation temperatures (PCR MP) are very specific for the given bacterial genome and may be used for strain characterisation and differentiation. The method may also be used for amplification and isolation of the less stable DNA fragments in a genome.     

Thermoregulatory responses to exercise at low ambient temperature performed after precooling or preheating procedures

Seven male skiers exercised for 30 min on a cycle ergometer at 50% of maximal oxygen uptake and an ambient temperature of 5 degrees C. The exercise was preceded either by cold exposure (PREC) or active warming-up (PREH). The data were compared with control exercise (CONT) performed immediately after entering the thermal chamber from a thermoneutral environment. Cold exposure resulted in negative heat storage (96.1 kJ.m-2, SE 5.9) leading to significantly lower rectal, mean body and mean skin temperatures at the onset of exercise in PREC, as compared to PREH and CONT. The PREC-PREH temperature differences were still significant at the end of the exercise period. During exercise in the PREC test, oxygen uptake was higher than in PREH test (32.8 ml.kg-1.min-1, SE 1.5 vs 30.5 ml.kg-1.min-1, SE 1.3, respectively). Heart rate showed only a tendency to be higher in PREC than in PREH and CONT tests. In the PREH test skin and body temperatures as well as sweat rate were already elevated at the beginning of exercise. Exercise-induced changes in these variables were minimal. Heat storage decreased with the duration of the exercise. Exercise at low ambient temperature preceded by a 30-min rest in a cold environment requires more energy than the same exercise performed after PREH.     

Occurrence of fatigue during sets of static squat jumps performed at a variety of loads

Research has identified that the optimal power load for static squat jumps (with no countermovement) is lower than the loads usually recommended for power training. Lower loads may permit the performance of additional repetitions before the onset of fatigue compared with heavier loads; therefore, the aim of this study was to determine the point of fatigue during squat jumps at various loads (0, 20, 40, 60% 1-repetition maximum [1RM]). Seventeen professional rugby league players performed sets of 6 squat jumps (with no countermovement), using 4 loading conditions (0, 20, 40, and 60% of 1RM back squat). Repeated measures analysis of variance revealed no significant differences (p > 0.05) in force, velocity, power, and displacement between repetitions, for the 0, 20, and 40% loading conditions. The 60% condition showed no significant difference (p > 0.05) in peak force between repetitions; however, velocity (1.12 + 0.10 and 1.18 + 0.11 m·s(-1)), power (3,385 + 343 and 3,617 + 396 W) and displacement (11.13 + 2.31 and 11.85 + 2.16 cm) were significantly (p < 0.02) lower during repetition 6 compared with repetition 2. These findings indicate that when performing squat jumps (with no countermovement) with a load <40% 1RM back squat, up to >6 repetitions can be completed without inducing fatigue and a minimum of 4-6 repetitions should be performed to achieve peak power output. When performing squat jumps (with no countermovement) with a load equal to the 60% 1RM only, 5 repetitions should be performed to minimize fatigue and ensure maintenance of velocity and power.     

Greater serum GH response to arm than to leg exercise performed at equivalent oxygen uptake

The aim of this study was to provide information concerning the mechanism of exercise-induced stimulation of growth hormone (GH) release in human subjects. For this reason serum GH as well as some hemodynamic variables and blood concentrations of noradrenaline (NA), insulin (IRI), lactate (LA), glucose (BG), and free fatty acids (FFA) were determined in seven healthy male subjects exercising on a bicycle ergometer with arms or legs and running on a treadmill at equivalent oxygen consumption levels. Significantly greater increases in serum GH concentration accompanied arm exercises than those observed during the leg exercises. This was accompanied by greater increases in heart rate, blood pressure, and plasma NA and blood lactate concentrations. Serum IRI decreased during both leg exercises and did not change during the arm exercise. There were no differences in BG and plasma FFA concentrations between the three types of exercise. The role of humoral and neural signals responsible for the greater GH response to arm exercise is discussed. The findings are consistent with the hypothesis that neural afferent signals sent by muscle "metabolic receptors" participate in the activation of GH release during physical exercise. It seems likely that the stimulation of these chemoreceptors is more pronounced when smaller muscle groups are engaged at a given work load. However, a contribution of efferent impulses derived from the brain motor centres to the control system of GH secretion during exercise is also possible.     

Review of in vivo static and ELF electric fields studies performed at Gazi Biophysics Department

In vivo effects of Static Electric and ELF Magnetic and Electric fields have been carried out for more than 20 years in the Bioelectromagnetic Laboratory at the Biophysics Department of the Medical Faculty of Gazi University. In this article, the results of in vivo ELF Electric field studies are presented as a review. Static and 50 Hz ELF (Extremely Low Frequency) Electric (E) fields effects on free radical synthesis, antioxidant enzyme level, and collagen synthesis were analyzed on tissues of guinea pigs, such as brain, liver, lung, kidney, spleen, testis, and plasma. Animals were exposed to static and ELF electric fields with intensities ranging from 0.3 kV/m to 1.9 kV/m in vertical and horizontal directions. Exposure periods were 1, 3, 5, 7, and 10 days. Electric fields were generated from a specially designed parallel plate capacitor system. The results indicate that the effects of electric fields on the tissues studied depend significantly on the type and magnitude of electric field and exposure period.     

Peak power, ground reaction forces, and velocity during the squat exercise performed at different loads

This study examined the changes in peak power, ground reaction force and velocity with different loads during the performance of the parallel squat movement. Twelve experienced male lifters (26.83 +/- 4.67 years of age) performed the standard parallel squat, using loads equal to 20, 30, 40, 50, 60, 70, 80, and 90% of 1 repetition maximum (1RM). Each subject performed all parallel squats with as much explosiveness as possible using his own technique. Peak power (PP), peak ground reaction force (PGRF), peak barbell velocity (PV), force at the time of PP (FPP), and velocity at the time of PP (VPP) were determined from force, velocity, and power curves calculated using barbell velocity and ground reaction force data. No significant differences were detected among loads for PP; however, the greatest PP values were associated with loads of 40 and 50% of 1RM. Higher loads produced greater PGRF and FPP values than lower loads (p < 0.05) in all cases except between loads equal to 60-50, 50-40, and 40-30% of 1RM for PGRF, and between loads equal to 70-60 and 60-50% of 1RM for FPP. Higher loads produced lower PV and VPP values than lower loads (p < 0.05) in all cases except between the 20-30, 70-80, and 80-90% of 1RM conditions. These results may be helpful in determining loads when prescribing need-specific training protocols targeting different areas of the load-velocity continuum.     

Peak force and rate of force development during isometric and dynamic mid-thigh clean pulls performed at various intensities

Eight male collegiate weightlifters (age: 21.2 +/- 0.9 years; height: 177.6 +/- 2.3 cm; and body mass: 85.1 +/- 3.3 kg) participated in this study to compare isometric to dynamic force-time dependent variables. Subjects performed the isometric and dynamic mid-thigh clean pulls at 30-120% of their one repetition maximum (1RM) power clean (118.4 +/- 5.5 kg) on a 61 x 121.9-cm AMTI forceplate. Variables such as peak force (PF) and peak rate of force development (PRFD) were calculated and were compared between isometric and dynamic conditions. The relationships between force-time dependent variables and vertical jump performances also were examined. The data indicate that the isometric PF had no significant correlations with the dynamic PF against light loads. On the one hand, there was a general trend toward stronger relationships between the isometric and dynamic PF as the external load increased for dynamic muscle actions. On the other hand, the isometric and dynamic PRFD had no significant correlations regardless of the external load used for dynamic testing. In addition, the isometric PF and dynamic PRFD were shown to be strongly correlated with vertical jump performances, whereas the isometric PRFD and dynamic PF had no significant correlations with vertical jump performances. In conclusion, it appears that the isometric and dynamic measures of force-time curve characteristics represent relatively specific qualities, especially when dynamic testing involves small external loads. Additionally, the results suggest that athletes who possess greater isometric maximum strength and dynamic explosive strength tend to be able to jump higher.     

Immunological effectiveness of a 2d revaccination against diphtheria performed at intervals extended up to 7-10 years

The present article deals with the data on the duration of antidiphtheria immunity, induced by the primary cycle of immunization with adsorbed DPT vaccine, with the aim of considering the problems of optimizing the immunization schedule for children. The prolongation of the interval before the second booster immunization to 7-10 years produces no negative influence on the effectiveness of immunization. Besides, the study has shown that after the main complex of immunizations with adsorbed DPT vaccine protective immunity to diphtheria is retained in 90.3-96.8% of children covered by this study for 9-10 years. These data point to high immunological effectiveness of adsorbed DPT vaccine and to the possibility of prolonging the intervals between booster immunizations to 10 years, as well as decreasing the number of booster immunizations in the immunization schedule for children.     

Twitch potentiation is greater after a fatiguing submaximal isometric contraction performed at short vs. long quadriceps muscle length.

Endurance time of a submaximal sustained contraction is longer when the muscle is fatigued in a shortened position. The aim of the present study was to compare central and peripheral mechanisms of fatigue after an isometric contraction of the knee extensor muscles performed at 20% maximal voluntary contraction (MVC) at two knee angles (35 degrees , short length vs. 75 degrees , long length; 0 degrees = full extension) until exhaustion. Eleven men (24 +/- 4 yr) attended two experimental randomized sessions. Endurance time was greater at 35 degrees compared with 75 degrees (974 +/- 457 vs. 398 +/- 144 s; P < 0.001) despite a similar reduction in knee extensor MVC (-28.4 +/- 16.0%, P < 0.001 vs. -27.6 +/- 18.8%, P < 0.001, respectively). Voluntary activation level was similarly depressed after the fatiguing contraction performed at the two muscle lengths (-19 +/- 16.7% at 35 degrees , P < 0.01 vs. -13.7 +/- 14.5% at 75 degrees , P < 0.01). After the fatiguing contraction, peak twitch potentiation was observed only at the short length (+31.8 +/- 17.6% at 35 degrees , P < 0.01 vs. +6.4 +/- 21.3% at 75 degrees , P > 0.05), whereas M-wave properties were similarly altered for the two angles. These results suggest that 1) central fatigue at task failure for a sustained isometric contraction was not dependent on the muscle length, and 2) the longer endurance time of a sustained isometric contraction performed at a shortened length is related to potentiation. It is suggested that the greater endurance time of a sustained isometric contraction observed at 35 degrees is related to the occurrence of potentiation at this short length, because central fatigue is similar at task failure for both tasks.