共查询到20条相似文献,搜索用时 15 毫秒
1.
Inside Cover: Polarimetric imaging of biological tissues based on the indices of polarimetric purity (J. Biophotonics 4/2018) 下载免费PDF全文
Albert Van Eeckhout Angel Lizana Enric Garcia‐Caurel José J. Gil Adrià Sansa Carla Rodríguez Irene Estévez Emilio González Juan C. Escalera Ignacio Moreno Juan Campos 《Journal of biophotonics》2018,11(4)
The cover shows the image enhancement of biological tissues provided by the Indices of Polarimetric Purity (IPPs). By measuring the Mueller matrix of a biological sample, using an imaging polarimeter, the IPPs are calculated. They are polarimetric indicators providing further synthetization of depolarizing samples and leading to enhanced image contrast for some biological structures. Once the IPPs are calculated, a pseudo‐colouring technique is applied for higher visualization. Further details can be found in the article by Albert Van Eeckhout et al. ( e201700189 )
2.
Front Cover: A theoretical‐experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms,assays, and analysis methods (J. Biophotonics 1/2018) 下载免费PDF全文
Silas J. Leavesley Brenner Sweat Caitlyn Abbott Peter Favreau Thomas C. Rich 《Journal of biophotonics》2018,11(1)
A hyperspectral image data cube acquired from HEK‐293 cells labeled with cytoplasmic and nuclear stains: Calcein Green and NucBlu. The top view (XY plane) displays three spectrally unmixed channels for cellular autofluorescence (red), Calcein Green (green), and NucBlue (blue). The Z axis shows spectral information, from low to high wavelength. The article by Leavesley and colleagues describes an approach for calculating the sensitivity of spectral imaging assays for detecting a fluorescence signature within a mix of other signatures or autofluorescence. Further details can be found in the article by Silas J. Leavesley et al. ( e201600227 ).
3.
Inside Back Cover: The conformation of bovine serum albumin adsorbed to the surface of single all‐dielectric nanoparticles following light‐induced heating (J. Biophotonics 7/2018) 下载免费PDF全文
Andrei A. Krasilin Katerina Volodina Arina A. Sukhova Mihail I. Petrov Dmitry A. Zuev Vyacheslav A. Dyachuk Valentin A. Milichko 《Journal of biophotonics》2018,11(7)
Germanium vs Silicon: All‐dielectric nanoparticles provides the heat resistance for proteins under light‐induced heating. Further details can be found in the article by Andrei A. Krasilin et al. ( e201700322 )
4.
Inside Front Cover: Enhanced volumetric imaging in 2‐photon microscopy via acoustic lens beam shaping (J. Biophotonics 2/2018) 下载免费PDF全文
Simonluca Piazza Paolo Bianchini Colin Sheppard Alberto Diaspro Martí Duocastella 《Journal of biophotonics》2018,11(2)
Two‐photon microscopy is the tool of choice for fluorescence imaging of deep tissues with high resolution, but can be limited in three‐dimensional acquisition speed and penetration depth. In this work, these issues are addressed by using an acoustic optofluidic lens capable of ultrafast beam shaping on a pixel basis. Driving the lens with different phase profiles enables high‐speed volumetric imaging, or enhanced signal‐to‐background for deeper penetration. Further details can be found in the article by Simonluca Piazza et al. ( e201700050 )
5.
Inside Front Cover: Near‐infrared bone densitometry: A feasibility study on distal radius measurement (J. Biophotonics 7/2018) 下载免费PDF全文
This study provides a simple method to detect human distal radius bone density based on near infrared (NIR) imaging. The information of bone mineral density can be measured by transluminational optical bone densitometric system. Compared to dual‐energy x‐ray absorptiometry (DXA) results in clinical trial, NIR images show a strong correlation to DXA. Further details can be found in the article by Chun Chung, Yu‐Pin Chen, Tsai‐Hsueh Leu, and Chia‐Wei Sun ( e201700342 ).
6.
Minimally invasive screening for colitis using attenuated total internal reflectance fourier transform infrared
spectroscopy 下载免费PDF全文
Jitto Titus Emilie Viennois Didier Merlin A. G. Unil Perera 《Journal of biophotonics》2017,10(3):465-472
This article describes a rapid, simple and cost‐effective technique that could lead to a screening method for colitis without the need for biopsies or in vivo measurements. This screening technique includes the testing of serum using Attenuated Total Reflectance Fourier Transform Infrared (ATR‐FTIR) spectroscopy for the colitis‐induced increased presence of mannose. Chronic (Interleukin 10 knockout) and acute (Dextran Sodium Sulphate‐induced) models for colitis are tested using the ATR‐FTIR technique. Arthritis (Collagen Antibody Induced Arthritis) and metabolic syndrome (Toll like receptor 5 knockout) models are also tested as controls. The marker identified as mannose uniquely screens and distinguishes the colitic from the non‐colitic samples and the controls. The reference or the baseline spectrum could be the pooled and averaged spectra of non‐colitic samples or the subject's previous sample spectrum. This shows the potential of having individualized route maps of disease status, leading to personalized diagnosis and drug management.
7.
Wei‐Jen Li Yung‐Tsan Chen Po‐Hao Huang Tsung‐Lin Yang Jian‐Jang Huang 《Journal of biophotonics》2017,10(1):92-97
Semiconductor nanocomposites provide advantages beyond the capability of typical fluorescent materials for cancer detection. In this work, nanowire‐based probes with dual color channels are employed to demonstrate the capacity of cancer cell detection. Purple emitting ZnO/antibody probes are applied to detect cancer cells and meanwhile TiO2/antibody probes with green light emission are applied to identify normal fibroblast cells. A series of quantitative analyses are conducted to verify the correlation between the concentrations of ZnO and TiO2 probes, cell numbers, and peak intensities of the PL spectra. The results provide a quantitative reference for developing nanowire‐based cancel cell probes.
8.
Inverse SORS for detecting a low Raman‐active turbid sample placed inside a highly Raman‐active diffusely scattering matrix – A feasibility study 下载免费PDF全文
Khan Mohd. Khan Surjendu B. Dutta Hemant Krishna Shovan K. Majumder 《Journal of biophotonics》2016,9(9):879-887
The broad range of applications of spatially‐offset Raman spectroscopy (SORS) were found to involve samples having only marginal differences in Raman cross‐sections between the surface and subsurface targets. We report the results of a feasibility study to evaluate the potential of the approach to identify the presence of a very low Raman‐active turbid sample placed inside a highly Raman‐active diffusely scattering matrix. Paraffin sandwiched tissue blocks prepared by embedding slices of chicken muscle tissue into solid paraffin blocks were employed as representative samples for the study. It was found that in contrast to the several millimetres of probing depth reported in the earlier applications, the Raman signatures of tissue were best recovered when it was located beneath the surface of the paraffin block at a depth of around a millimetre, beyond which the quality of recovery was increasingly poorer. However, the probing depth could be further increased by increasing the thickness of the embedded tissue sections. The results clearly suggest that though the probing depth achievable under the current condition is less than that found in previous applications, nevertheless it is sufficient for various other applications that would not require probing as deep as was required earlier.
9.
Shuting Fan Benjamin S. Y. Ung Edward P. J. Parrott Vincent P. Wallace Emma Pickwell‐MacPherson 《Journal of biophotonics》2017,10(9):1143-1151
We use terahertz imaging to measure four human skin scars in vivo. Clear contrast between the refractive index of the scar and surrounding tissue was observed for all of the scars, despite some being difficult to see with the naked eye. Additionally, we monitored the healing process of a hypertrophic scar. We found that the contrast in the absorption coefficient became less prominent after a few months post‐injury, but that the contrast in the refractive index was still significant even months post‐injury. Our results demonstrate the capability of terahertz imaging to quantitatively measure subtle changes in skin properties and this may be useful for improving scar treatment and management.
10.
Three‐dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community 下载免费PDF全文
Alessandra Costa Alberto Diaspro Susanne Melzer Attila Tarnok 《Journal of biophotonics》2017,10(1):24-45
Tissue engineering/regenerative medicine (TERM) is an interdisciplinary field that applies the principle of engineering and life sciences to restore/replace damaged tissues/organs with in vitro artificially‐created ones. Research on TERM quickly moves forward. Today newest technologies and discoveries, such as 3D‐/bio‐printing, allow in vitro fabrication of ex‐novo made tissues/organs, opening the door to wide and probably never‐ending application possibilities, from organ transplant to drug discovery, high content screening and replacement of laboratory animals. Imaging techniques are fundamental tools for the characterization of tissue engineering (TE) products at any stage, from biomaterial/scaffold to construct/organ analysis. Indeed, tissue engineers need versatile imaging methods capable of monitoring not only morphological but also functional and molecular features, allowing three‐dimensional (3D) and time‐lapse in vivo analysis, in a non‐destructive, quantitative, multidimensional analysis of TE constructs, to analyze their pre‐implantation quality assessment and their fate after implantation. This review focuses on the newest developments in imaging technologies and applications in the context of requirements of the different steps of the TERM field, describing strengths and weaknesses of the current imaging approaches.
11.
Complex refractive index of normal and malignant human colorectal tissue in the visible and near‐infrared 下载免费PDF全文
Panagiotis Giannios Spyridon Koutsoumpos Konstantinos G. Toutouzas Maria Matiatou George C. Zografos Konstantinos Moutzouris 《Journal of biophotonics》2017,10(2):303-310
A multi‐wavelength prism coupling refractometer is utilized to measure the angular reflectance of freshly excised human intestinal tissue specimens. Based on reflectance data, the real and imaginary part of the refractive index is calculated via Fresnel analysis for three visible (blue, green, red) and two near‐infrared (963 nm and 1551 nm) wavelengths. Averaged values of the complex refractive index and corresponding Cauchy dispersion fits are given for the mucosa, submucosa and serosa layers of the colorectal wall at the normal state. The refractive constants of tumorous and normal mucosa are then cross‐compared for the indicative cases of one patient diagnosed with a benign polyp and three patients diagnosed with adenocarcinomas of different phenotype. Significant index contrast exists between the normal and diseased states, indicating the potential use of refractive index as a marker of colorectal dysplasia.
12.
Back Cover: Joint tagging assisted fluctuation nanoscopy enables fast high‐density super‐resolution imaging (J. Biophotonics 9/2018) 下载免费PDF全文
Based on multicolor quantum dots (QDs) labeling, the joint tagging assisted super‐resolution radial fluctuation (JT‐SRRF) nanoscopy achieves high‐fidelity super‐resolution imaging of subcellular microtubules and fast live‐cell parallel tracking of cholera toxin subunit B (CTB) induced lipid clusters spatially distributed below the optical diffraction limit. This method paves the way for fast high‐density parallel tracking, which is especially beneficial for the investigation of the intensive dynamics in live‐cell applications. Further details can be found in the article by Zhiping Zeng, Jing Ma, Peng Xi, and Canhua Xu ( e201800020 ).
13.
Back Cover: In‐vitro analysis of early calcification in aortic valvular interstitial cells using Laser‐Induced Breakdown Spectroscopy (LIBS) (J. Biophotonics 1/2018) 下载免费PDF全文
Quantitative laser‐induced breakdown spectroscopy (LIBS) is successfully used for in‐vitro analysis of early stage calcification in aortic valvular interstitial cells (VICs). LIBS results indicate 5‐fold improvement in the detection limit of calcium deposition in VICs over cell histology techniques involving staining and colorimetric calcium assays. These results can establish LIBS at the forefront of early detection of calcification in VICs for pathological studies on Calcific Aortic Valve Disease (CAVD). Further details can be found in the article by Seyyed Ali Davari et al. ( e201600288 ).
14.
Stephan Stöckel Susann Meisel Björn Lorenz Sandra Kloß Sandra Henk Stefan Dees Elvira Richter Sönke Andres Matthias Merker Ines Labugger Petra Rösch Jürgen Popp 《Journal of biophotonics》2017,10(5):727-734
In this study, Raman microspectroscopy has been utilized to identify mycobacteria to the species level. Because of the slow growth of mycobacteria, the per se cultivation‐independent Raman microspectroscopy emerges as a perfect tool for a rapid on‐the‐spot mycobacterial diagnostic test. Special focus was laid upon the identification of Mycobacterium tuberculosis complex (MTC) strains, as the main causative agent of pulmonary tuberculosis worldwide, and the differentiation between pathogenic and commensal nontuberculous mycobacteria (NTM). Overall the proposed model considers 26 different mycobacteria species as well as antibiotic susceptible and resistant strains. More than 8800 Raman spectra of single bacterial cells constituted a spectral library, which was the foundation for a two‐level classification system including three support vector machines. Our model allowed the discrimination of MTC samples in an independent validation dataset with an accuracy of 94% and could serve as a basis to further improve Raman microscopy as a first‐line diagnostic point‐of‐care tool for the confirmation of tuberculosis disease.
15.
Inside Back Cover: Development of a 3‐dimensional tissue lung phantom of a preterm infant for optical measurements of oxygen—Laser‐detector position considerations (J. Biophotonics 3/2018) 下载免费PDF全文
Jim Larsson Peilang Liao Patrik Lundin Emilie Krite Svanberg Johannes Swartling Märta Lewander Xu Joakim Bood Stefan Andersson‐Engels 《Journal of biophotonics》2018,11(3)
The picture depicts the different 3d‐printed organs, thorax, lungs, heart and bone. Assembled it is used as an optical phantom of a preterm infant for performing percutaneous optical measurements of the gas content in the lungs. In order to simulate the optical properties of the tissue, the heart and thorax can be filled with liquid phantoms, a mixture of Intralipid and Indian Ink. Further details can be found in the article by Jim Larsson et al. ( e201700097 ).
16.
Back Cover: Non‐invasive optical method for real‐time assessment of intracorneal riboflavin concentration and efficacy of corneal cross‐linking (J. Biophotonics 7/2018) 下载免费PDF全文
Giuseppe Lombardo Valentina Villari Norberto L. Micali Nancy Leone Cristina Labate Maria P. De Santo Marco Lombardo 《Journal of biophotonics》2018,11(7)
We disclose a theranostic device for performing image‐guided riboflavin/UV‐A corneal cross‐linking. The device determines treatment efficacy by real time monitoring of riboflavin concentration in the corneal stroma. The study shows efficacy of the device in eye bank human donor tissues. Further details can be found in the article by Giuseppe Lombardo et al. ( e201800028 )
17.
Inside Back Cover: Multiphoton dynamic imaging of the effect of chronic hepatic diseases on hepatobiliary metabolism in vivo (J. Biophotonics 9/2018) 下载免费PDF全文
Chih‐Ju Lin Sheng‐Lin Lee Wei‐Hsiang Wang Vladimir A. Hovhannisyan Yao‐De Huang Hsuan‐Shu Lee Chen‐Yuan Dong 《Journal of biophotonics》2018,11(9)
In vivo multiphoton imaging was used to map changes in hepatobiliary metabolism in liver fibrosis (left column) and hepatocellular carcinoma (right column). The top row shows the maps of kinetic rate constant of the uptake and esterase processing while the bottom row shows that of bile canalicular excretion of xenobiotics. Further details can be found in the article by Chih‐Ju Lin, Sheng‐Lin Lee, Wei‐Hsiang Wang, et al. ( e201700338 ).
18.
Front Cover: Assessment of trans‐scleral iontophoresis delivery of lutein to the human retina (J. Biophotonics 3/2018) 下载免费PDF全文
Marco Lombardo Valentina Villari Norberto Micali Pierre Roy Sara H. Sousa Giuseppe Lombardo 《Journal of biophotonics》2018,11(3)
Trans‐scleral iontophoresis device was shown to be effective for in‐situ delivery of lutein to the retina of human donor eyes. After treatment, Resonance Raman Spectroscopy measurements demonstrated that lutein greatly enriched the inner sclera, choroid and retina. Clinical studies are going to prove if the methodology would be a valuable approach to enrich the human macular pigment and prevent local oxidative damage in patients at risk of AMD progression. Further details can be found in the article by Marco Lombardo et al. ( e201700095 ).
19.
Back Cover: Autofluorescence and white light imaging‐guided endoscopic Raman and diffuse reflectance spectroscopy for in vivo nasopharyngeal cancer detection (J. Biophotonics 4/2018) 下载免费PDF全文
Jianji Pan Zhihong Xu Rong Chen Shangyuan Feng Guannan Chen Yongzeng Li Michael Short Jianhua Zhao Yasser Fawzy Haishan Zeng 《Journal of biophotonics》2018,11(4)
An integrated 4‐modality endoscopy system combining white light imaging, autofluorescence imaging, diffuse reflectance spectroscopy and Raman spectroscopy technologies was developed for in vivo endoscopic nasopharyngeal cancer detection. Both high diagnostic sensitivity (98.6%) and high specificity (95.1%) for differentiating cancer from normal tissue sites were achieved using this system combined with multivariate diagnostic algorithm, demonstrating great potential for improving real‐time, in vivo diagnosis of cancer at endoscopy. Further details can be found in the article by Duo Lin et al. ( e201700251 )
20.
Inside Front Cover: Combining hyperspectral imaging and chemometrics to assess and interpret the effects of environmental stressors on zebrafish eye images at tissue level (J. Biophotonics 3/2018) 下载免费PDF全文
Víctor Olmos Mònica Marro Pablo Loza‐Alvarez Demetrio Raldúa Eva Prats Francesc Padrós Benjamin Piña Romà Tauler Anna de Juan 《Journal of biophotonics》2018,11(3)
Raman images were used to study the effect of the contaminant chlorpyriphos‐oxon on zebrafish eye samples. Multivariate Curve Resolution‐Alternating Least Squares (MCR‐ALS) was used to obtain the distribution maps and spectral signatures of biological components present in the images analyzed. The use of MCRALS spectral signatures as starting information for Partial Least Squares‐Discriminant Analysis allowed statistical assessment of the effect of the contaminant at a specific tissue level. Further details can be found in the article by Víctor Olmos et al. ( e201700089 ).