转录因子结合位点生物信息学研究进展

By using genome in situ hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum × G. bickii with genomic DNA (gDNA) of G. bickii as a probe, two sets of chromosomes, consisting of 26 chromosomes each, were easily distinguished from each other by their distinctive hybridization signals. GISH analysis directly proved that the hybrid G.arboreum×G. bickii is an allotetraploid amphiploid. The karyotype formula of the species was 2n = 4x = 52 = 46m (4sat) + 6sm (4sat). We identified four pairs of satellites with two pairs in each sub-genome. FISH analysis using 45S rDNA as a probe showed that the cross G. arboreum×G. bickii contained 14 NORs. At least five pairs of chromosomes in the G sub-genome showed double hybridization (red and blue) in their long arms, which indicates that chromatin introgression from the A sub-genome had occurred.     

DNA保守序列识别算法的并行化和MPI集群环境构建

DNA序列中保守序列的识别需要较大的计算量。开发了一个转录因子结合位点识别的并行算法,能够从多条DNA序列中识别指定长度的序列模式。算法使用概率模型进行序列模式保守性的度量,利用迭代过程实现保守序列的搜索。使用C编程结合MPI消息传递模型开发了相应的程序,并在Windows平台下构建了一个3节点的集群环境,利用20个长度均为200的序列数据集进行测试,实现了模体识别工作,结果表明并行算法使模体识别的效率得到提高。