共查询到20条相似文献,搜索用时 31 毫秒
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人Boule基因启动子区结合蛋白的生物信息学分析 总被引:1,自引:0,他引:1
目的:对精子发生RNA结合蛋白Boule基因启动子区结合蛋白进行生物信息学分析。方法:从基因参考序列数据库获取Boule基因启动子区序列,使用TFSEARCH程序对启动子序列中的转录因子结合位点进行预测。结果:成功获得长度为2kb的人Boule基因启动子区序列。该启动子区Thresholdscore〉90的共有60个转录因子结合位点,涉及sox家族、GATA结合蛋白家族、热休克因子家族、锌指蛋白Kruppel家族、POU家族、runt家族、同源异型框基因家族、TALE类同源结构蛋白家族、转录因子螺旋环螺旋家族、IKAROS家族、FOX家族11个家族的转录因子和3个TATAbox。结论:Boule基因表达的调控是在一定时间或空间上、一种或多种调节蛋白作用的复杂过程。调控Boule基因表达的转录因子绝大部分与胚胎发育、性别决定、个体生长密切相关。 相似文献
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Wu K Yang Y Wang C Davoli MA D'Amico M Li A Cveklova K Kozmik Z Lisanti MP Russell RG Cvekl A Pestell RG 《The Journal of biological chemistry》2003,278(51):51673-51684
The vertebrate homologues of Drosophila dachsund, DACH1 and DACH2, have been implicated as important regulatory genes in development. DACH1 plays a role in retinal and pituitary precursor cell proliferation and DACH2 plays a specific role in myogenesis. DACH proteins contain a domain (DS domain) that is conserved with the proto-oncogenes Ski and Sno. Since the Ski/Sno proto-oncogenes repress AP-1 and SMAD signaling, we hypothesized that DACH1 might play a similar cellular function. Herein, DACH1 was found to be expressed in breast cancer cell lines and to inhibit transforming growth factor-beta (TGF-beta)-induced apoptosis. DACH1 repressed TGF-beta induction of AP-1 and Smad signaling in gene reporter assays and repressed endogenous TGF-beta-responsive genes by microarray analyses. DACH1 bound to endogenous NCoR and Smad4 in cultured cells and DACH1 co-localized with NCoR in nuclear dotlike structures. NCoR enhanced DACH1 repression, and the repression of TGF-beta-induced AP-1 or Smad signaling by DACH1 required the DACH1 DS domain. The DS domain of DACH was sufficient for NCoR binding at a Smad4-binding site. Smad4 was required for DACH1 repression of Smad signaling. In Smad4 null HTB-134 cells, DACH1 inhibited the activation of SBE-4 reporter activity induced by Smad2 or Smad3 only in the presence of Smad4. DACH1 participates in the negative regulation of TGF-beta signaling by interacting with NCoR and Smad4. 相似文献
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Rauen T Benedyk K Juang YT Kerkhoff C Kyttaris VC Roth J Tsokos GC Tenbrock K 《The Journal of biological chemistry》2011,286(37):32366-32372
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Yiting Yu Yongkai Mo David Ebenezer Sanchari Bhattacharyya Hui Liu Sriram Sundaravel Orsolya Giricz Sandeep Wontakal Jessy Cartier Bennett Caces Andrew Artz Sangeeta Nischal Tushar Bhagat Kathleen Bathon Shahina Maqbool Oleg Gligich Masako Suzuki Ulrich Steidl Lucy Godley Art Skoultchi John Greally Amittha Wickrema Amit Verma 《The Journal of biological chemistry》2013,288(13):8805-8814
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Kosaka-Suzuki N Suzuki T Pugacheva EM Vostrov AA Morse HC Loukinov D Lobanenkov V 《The Journal of biological chemistry》2011,286(31):27378-27388
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