Diet of Yellow-legged Gull ( Larus michahellis ) chicks along the Spanish Western Mediterranean coast: the relevance of refuse dumps

In recent decades, the Yellow-legged Gull (Larus michahellis) has become a problematic species in many Mediterranean countries, mainly because it interferes with human interests. However, this gull also has a negative impact on several other bird species, many of which are classified as endangered. Two different European Union Action Plans are currently under development with the aim of decreasing the availability of food derived from human activities, such as garbage and fishery discards, which are considered to be the main causes of the superpopulations of this gull. Here, we describe the diet of Yellow-legged Gull chicks, with particular emphasis on establishing the dependence of each population on refuse dumps, in order to forecast changes in gull population dynamics in response to the management decisions being implemented. We sampled four colonies along the Western Mediterranean in Spain: the Medes Islands, the Ebro Delta, the Columbretes Islands, and Mazarrón Island. To elucidate their feeding ecology and to avoid obtaining a discrete estimation from a single sampling, we collected regurgitates from each colony three times throughout the chick-rearing period. Slightly differential feeding habits were observed between chick age classes. Younger chicks in all four colonies tended to be consistently provisioned with smaller prey such as invertebrates. Distinct uses of several foraging habitats among localities were observed. In particular, the use of refuse dumps was common and abundant in two of the colonies: the Medes and Mazarrón Islands. As a consequence of current management strategies, generalized reductions in Yellow-legged Gull populations and increases in the consumption of alternative food resources to those of fishery discards and refuse scraps are expected. Finally, we predict that decreased food availability will force some gulleries to increase predation on endangered species, thereby raising a conservation concern. X. Ruiz: deceased on 27 April 2008.     

Past and present trophic position and decadal changes in diet of Yellow-legged Gull in the Azores Archipelago, NE Atlantic

This study evaluates the trophic position of adult Yellow-legged Gulls Larus michahellis atlantis resident in the Azores archipelago in the past (1921–1928) and in the present (2009–2010), and analyses the decadal variation in the diet of breeding birds from the 1990s to the 2000s for three main colonies (Topo Islet, Baixo Islet and Mistério da Prainha). Using mixing models, we compared stable isotope signatures of nitrogen and carbon in adult breast feathers between birds from 1921 to 1928 (held in museum collections) and 2009 to 2010, jointly with both isotopic signatures of their main prey groups (fish, goose barnacles (Lepas anatifera), seabirds, mammals and refuse). The diet of breeding birds was analysed using pellets collected in 1989, 1995, 1996, 2004, 2009 and 2010. Stable isotopes analysis (SIA) results were in accordance with the results provided from the analysis of pellets, showing a relatively recent and significant change in the diet of adult gulls. In particular, SIA revealed a significant decrease in the trophic position of Yellow-legged Gulls in the Azores, over the last 89 years in response to the decrease in the consumption of seabirds and fish and, an increase in the consumption of marine invertebrates (goose barnacles) and refuse. The analysis of pellets confirmed the significant decrease in the fish ingested, whereas the ingestion of lower trophic level prey (i.e. goose barnacles, mammals and refuse) increased. Both methods reflect the feeding plasticity and opportunistic foraging behavior of this species, and are in accordance with patterns described for continental Europe.     

Habitat associations and breeding success of the Chaffinch Fringilla coelebs

Capsule Population trends for Chaffinch on farmland are unlikely to be explained by their preference for non-crop habitats alone.

Aims To investigate the importance of non-cropped habitats for Chaffinch territory distribution, breeding success and foraging habitat selection in Scottish farmland.

Methods Territory distribution, nesting success and foraging behaviour of adults feeding chicks at the nest were recorded and related to habitat composition on two Scottish lowland farms.

Results Higher Chaffinch territory densities were associated with the presence of hedgerows, trees and grass leys, whereas lower densities were found adjacent to winter Barley. Nests were predominantly located in conifer trees and hedgerows. Trees were selected preferentially for foraging. Poor breeding success was detected in nests associated with cereal crops and wider field margins. Farmland in Scotland supported much lower territory densities than in England.

Conclusion Chaffinches rely predominantly on non-cropped habitats for territory establishment, nesting and foraging habitats. Differences in habitat composition between England and Scotland may explain differences in territory densities. However, the Chaffinch's preference for non-crop habitats is unlikely to explain its population trends alone. Immigration from other habitats and/or a decrease in inter-specific competition for resources may also have contributed to the increase in the national farmland Chaffinch population.     

The impact of vegetation characteristics and prey availability on breeding habitat use and diet of Little Owls Athene noctua in Central European farmland

Capsule Foraging habitats are determined by vegetation characteristics rather than the availability of insect prey.

Aims To determine the diet composition of Little Owls in relation to the availability of insects at foraging sites, and to elucidate the main factors determining the owls’ habitat choice.

Methods The feeding ecology of Little Owls was studied during the 2002 breeding season in the agricultural landscape of western Bohemia (Czech Republic), where its population is in decline. Diet composition was determined by pellet analysis. Insect availability was studied using pitfall traps in the two most important Little Owl foraging habitats. For both habitats, we assessed the main vegetative characteristics (average and maximum vegetation height, vegetation density).

Results Based on number, insects were the most dominant prey, followed by small mammals; based on weight, insects comprised only a minor part of the diet. Among insect prey, Carabidae beetles were the most abundant. The proportion of insect numbers was strongly positively correlated with advancing day of the season and negatively correlated with the proportion of vertebrates. Although the highest densities of Carabidae were found in cornfields Little Owls significantly preferred grassland habitats, probably because of the lower vegetation cover.

Conclusions The availability of short sward vegetation in grassland habitats during the breeding season may play a key role in the conservation of Little Owls in central European farmland.     

The Origin of Winter Visitors to the British Isles 7. Fieldfare (Turdus pilaris)

Capsule Large‐scale intensification of agricultural management during the past 50 years has resulted in a reduction of invertebrate abundance and higher and denser ground vegetation. Food availability for insectivorous birds foraging on the ground has been negatively affected, but the interactions between birds and their food availability are complex and often species‐specific. Populations of Wrynecks Jynx torquilla are declining all over Europe, possibly because of reduced accessibility to their main prey, ground‐dwelling ants, due to higher and denser ground vegetation. However, it is not clear which ground vegetation structures are tolerated by foraging Wrynecks and which habitats are preferred.

Aims To identify the optimal ground vegetation structure and the main habitat types in which Wrynecks search for food.

Method We radiotracked seven Wrynecks in high‐intensity farmland in Switzerland to study foraging habitat use during the reproduction season. Several habitat variables were mapped at each foraging location and compared with locations selected randomly within individual home ranges.

Results Wrynecks preferentially foraged at places with ≥50% bare ground. Vegetation height was not important. Older fruit tree plantations and fallow land were the preferred foraging habitats.

Conclusion Conservation measures should concentrate on preserving semi‐open agricultural landscape matrices with loose ground vegetation cover to provide suitable foraging conditions. This can be achieved even in intensively managed farmland as illustrated in this study.     

Varying foraging patterns in response to competition? A multicolony approach in a generalist seabird

Reducing resource competition is a crucial requirement for colonial seabirds to ensure adequate self‐ and chick‐provisioning during breeding season. Spatial segregation is a common avoidance strategy among and within species from neighboring breeding colonies. We determined whether the foraging behaviors of incubating lesser black‐backed gulls (Larus fuscus) differed between six colonies varying in size and distance to mainland, and whether any differences could be related to the foraging habitats visited. Seventy‐nine incubating individuals from six study colonies along the German North Sea coast were equipped with GPS data loggers in multiple years. Dietary information was gained by sampling food pellets, and blood samples were taken for stable isotope analyses. Foraging patterns clearly differed among and within colonies. Foraging range increased with increasing colony size and decreased with increasing colony distance from the mainland, although the latter might be due to the inclusion of the only offshore colony. Gulls from larger colonies with consequently greater density‐dependent competition were more likely to forage at land instead of at sea. The diets of the gulls from the colonies furthest from each other differed, while the diets from the other colonies overlapped with each other. The spatial segregation and dietary similarities suggest that lesser black‐backed gulls foraged at different sites and utilized two main habitat types, although these were similar across foraging areas for all colonies except the single offshore island. The avoidance of intraspecific competition results in colony‐specific foraging patterns, potentially causing more intensive utilization of terrestrial foraging sites, which may offer more predictable and easily available foraging compared with the marine environment.     

Breeding Yellow-legged Gulls increase consumption of terrestrial prey after landfill closure

The ecology of opportunistic foragers can be highly dependent on anthropogenic food sources, such as landfills, resulting in changes in several ecological and demographic aspects. The total closure of several landfill sites and the use of deterrence systems to prevent access to the remaining open landfill sites in a region in the northern Iberian Peninsula provided an excellent opportunity to evaluate the consequences of landfills on the trophic ecology of an opportunistic forager, the Yellow-legged Gull Larus michahellis, using these resources. Based on stable isotope analyses, we used mixing models to estimate changes in diet before and after the closure of the majority of landfills in the breeding and the non-breeding season. We found a decrease in the consumption of food from landfills after their closure, which was accompanied by an increase in feeding on terrestrial prey (mostly earthworms), but only in the breeding season. Interestingly, we observed no increase in marine prey consumption after the landfill closures. In winter there was a decrease in terrestrial prey consumption, whereas the consumption of marine and, despite their reduce availability, landfill resources increased. Thus, and unlike when all the landfills were open, we detected significant trophic differences between breeding and non-breeding seasons. Additionally, we found significant trophic differences among colonies that were quite close to each other, but not between breeding adults and chicks. In conclusion, landfill closure or the use of deterrence systems had a direct impact on the trophic ecology of Yellow-legged Gulls; loss of refuse was mainly compensated for by prey of terrestrial origin when breeding, but not in winter. Thus, this species may experience foraging constraints in winter with potential effects on other life-history aspects including their dispersal, breeding and survival that needs further evaluation.     

Intercolony variations in movement patterns and foraging behaviors among herring gulls (Larus argentatus) breeding in the eastern Wadden Sea

Herring gulls (Larus argentatus) are opportunistic predators that prefer to forage in the intertidal zone, but an increasing degree of terrestrial foraging has recently been observed. We therefore aimed to analyze the factors influencing foraging behavior and diet composition in the German Wadden Sea. Gulls from three breeding colonies on islands at different distances from the mainland were equipped with GPS data loggers during the incubation seasons in 2012–2015. Logger data were analyzed for 37 individuals, including 1,115 foraging trips. Herring gulls breeding on the island furthest from the mainland had shorter trips (mean total distance = 12.3 km; mean maximum distance = 4.2 km) and preferred to feed on the tidal flats close to the colony, mainly feeding on common cockles (Cerastoderma edule) and shore crabs (Carcinus maenas). In contrast, herring gulls breeding close to the mainland carried out trips with a mean total distance of 26.7 km (mean maximum distance = 9.2 km). These gulls fed on the neobiotic razor clams (Ensis leei) in the intertidal zone, and a larger proportion of time was spent in distant terrestrial habitats on the mainland, feeding on earthworms. δ¹³C and δ¹⁵N values were higher at the colony furthest from the mainland and confirmed a geographical gradient in foraging strategy. Analyses of logger data, pellets, and stable isotopes revealed that herring gulls preferred to forage in intertidal habitats close to the breeding colony, but shifted to terrestrial habitats on the mainland as the tide rose and during the daytime. Reduced prey availability in the vicinity of the breeding colony might force herring gulls to switch to feed on razor clams in the intertidal zone or to use distant terrestrial habitats. Herring gulls may thus act as an indicator for the state of the intertidal system close to their breeding colony.     

Trophic strategies of Yellow-legged Gull Larus michahellis on oceanic islands surrounded by deep waters

Capsule: The diet of Yellow-legged Gulls Larus michahellis on an oceanic island, surrounded by deep waters without a wide shelf, was mainly composed of terrestrial invertebrates.

Aims: To study the trophic ecology of Yellow-legged Gulls on an island surrounded by deep waters, to quantify the importance of terrestrial prey items and their availability, and to evaluate the relative importance of nutritional values of terrestrial and marine resources.

Methods: Diet was monitored for one year. We assessed the relative contribution of the main prey items and their macronutrients through the study of pellets and faeces.

Results: Terrestrial invertebrates were the most frequently consumed prey items (frequency of occurrence (FO) 67%), followed by marine fish (Osteichthyes FO 33%). Coleoptera and Orthoptera were the most consumed terrestrial invertebrates and provided a high nutritional value. The rate of consumption of terrestrial invertebrates varied in synchrony with the breeding season, being higher in spring, indicating their potential importance for reproduction.

Conclusion: It is unusual that terrestrial invertebrates constitute a large proportion of the diet of large gulls, but they seemed to cover their trophic energy requirements during the reproductive period.     

Foraging in space and time structure an African small mammal community

We used live-trapping and foraging to test for the effect of habitat selection and diet on structuring a community of six small mammals and one bird within the Soutpansberg, South Africa. We established grids that straddled adjacent habitats: woodland, rocky hillside, and grassland. Trapping and foraging were used to estimate abundance, habitat use, and species-specific foraging costs. The species with the highest abundance and foraging activity in a habitat, activity time, or food was considered the most efficient and presumed to have a competitive advantage. All species exhibited distinct patterns of spatial and temporal habitat preference which provided the main mechanism of coexistence, followed by diet selection. The study species were organized into three assemblages (α diversity): grassland, Rhabdomys pumilio, Dendromus melanotis, and Mus minutoides.; woodland, Aethomys ineptus and Micaelamys namaquensis; and rock-dwelling, M. namaquensis and Elephantulus myurus. Francolinus natalensis foraged in open rocky areas and under wooded islands within the grassland. Species organization across the habitats suggested that feeding opportunities are available within all habitats; however, distinct habitat preferences resulted from differing foraging aptitudes and efficiencies of the competing species. At Lajuma, species distribution and coexistence are promoted through distinct habitat preferences that were shaped by competition and species-specific foraging costs. The combination of trapping and foraging provided a mechanistic approach that integrates behavior into community ecology by ‘asking’ the animal to reveal its perspective of the environment. Using spatial and temporal foraging decisions—as behavioral indicators—enables us to guide our understanding for across-taxa species coexistence.     

Parental provisioning behaviour in Pied Flycatchers Ficedula hypoleuca is well adjusted to local conditions in a mosaic of deciduous and coniferous habitat

Capsule Pied Flycatchers are better able than Great Tits to adjust their feeding behaviour to varying conditions in the same area.

Aims Great Tits breeding in a mosaic of deciduous and coniferous forests in the northern temperate region exhibit consistently lower breeding success in their preferred deciduous habitat than in coniferous habitat. This was explained by the unexpectedly poor nestling feeding conditions in deciduous forests of this region. We studied whether the same paradox applies to Pied Flycatchers that occupy the same habitats in the same area.

Methods Parental provisioning behaviour was studied using video‐recording and experimental manipulation. Caterpillar abundance and basic breeding parameters were measured in different habitat types.

Results Parental provisioning frequency and the proportion of caterpillars in nestling diet was lower, while food objects were on average larger, in coniferous compared with deciduous habitat. However, the total volume of caterpillars and adult Lepidoptera delivered to nestlings did not differ between habitats. In contrast to Great Tits, offspring body parameters in Pied Flycatchers did not differ between habitat types.

Conclusions These results demonstrate how the relative suitability of particular habitat types varies between species and is dependent upon geographical location.     

Use of field margins managed under an agri-environment scheme by foraging Barn Swallows Hirundo rustica

Capsule: Barn Swallows Hirundo rustica were more likely to forage along arable field margins that were enhanced with wildflowers or legumes than control grass margins.

Aims: To determine if foraging Barn Swallows displayed preferences for specific arable field boundary habitats (grass margins versus floristically enhanced margins) that were managed as part of an agri-environment scheme. We also aim to determine how Barn Swallow food abundance related to these habitats.

Methods: Two foraging activity surveys took place on all grass and floristically enhanced margins (n?=?56) present within the 600 m foraging range of seven Barn Swallow colonies during June and July 2016. Margin habitat use was measured by recording the presence/absence of foraging individuals during surveys, the total number of individuals and by calculating an index of foraging activity. Habitat information relating to adjacent boundary type, transect crop type and neighbouring crop type were also recorded.

Results: Foraging Barn Swallows were significantly more likely to be recorded when survey transects included a floristically enhanced margin, but there was no significant impact of floristically enhanced margins on the total number of individuals recorded or on the index of foraging activity. Foraging activity was higher along grass verges and hedgerows when compared to treelines and was positively related to length weighted Diptera abundance (a measure of food biomass).

Conclusion: Our results suggest that there may be a role for floristically enhanced margins in the conservation of Barn Swallows on arable farmland. More research, however, is needed to determine whether invertebrate-rich agri-environment scheme habitats can influence colony size or improve the breeding success of this species.     

Avian spatial segregation at edges and interiors of urban parks in Madrid, Spain

Few studies have investigated the effects of urban landscape composition on avian habitat selection at urban-park edges. I assessed how the number of species, density of guilds, and density of individual species varied between edge and interior habitats in six large wooded parks in Madrid (Spain), and analysed such patterns in relation to habitat structure, car traffic, and pedestrian traffic. Few differences in habitat structure were found; whereas car and pedestrian traffic were significantly higher at edges. Species foraging in trees and on the ground, and nesting in trees and in tree cavities had lower numbers and breeding densities at edges, probably as a result of the disturbance from traffic noise and pedestrians. Species highly habituated to human activities (House Sparrows Passer domesticus and Rock Doves Columba livia) displayed opposite patterns, with higher breeding densities at urban-park edges, probably due to their higher foraging opportunities (refuse, people leftovers, deliberate feeding) and nest site availability in adjacent buildings. Urbanisation sprawl may increase the prevalence of edge specialists and diminish the representation of species with specific habitat requirements.     

Identifying habitat-driven spatial variation in colony size of Herring Gulls Larus argentatus

Capsule: Herring Gull Larus argentatus colonies located along coastlines that are relatively sheltered from wave impact, associated with high intertidal prey availability, and with extensive farmland habitat in the vicinity, have increased in size over the last three decades.

Aims: To investigate potential habitat drivers, associated with local food availability, of variation in Herring Gull colony sizes.

Methods: Colony sizes were compared for 68 Herring Gull colonies across southwest Scotland and Northern Ireland to relate to the availability of potential foraging habitats surrounding each colony.

Results: Changes in Herring Gull colony sizes were spatially clustered, with between-colony differences in colony size related to coastline exposure and amount of farmland within the foraging range. At the end of the census period, colonies located in areas with the greatest availability of intertidal prey and farmland habitat were larger compared to those that were not.

Conclusion: Spatially clustered variation in changes of the size of Herring Gull colonies indicates that neighbouring colonies experienced similar environmental conditions and highlights the importance of intertidal and farmland habitats in buffering against declines. This result emphasizes the need for area-specific management for this species of conservation concern.     

Do invasive species undergo metapopulation dynamics? A case study of the invasive Caspian gull,Larus cachinnans,in Poland

Aim The mechanisms of initial dispersal and habitat occupancy by invasive alien species are fundamental ecological problems. Most tests of metapopulation theory are performed on local population systems that are stable or in decline. In the current study we were interested in the usefulness of metapopulation theory to study patch occupancy, local colonization, extinction and the abundance of the invasive Caspian gull (Larus cachinnans) in its initial invasion stages. Location Waterbodies in Poland. Methods Characteristics of the habitat patches (waterbodies, 35 in total) occupied by breeding pairs of Caspian gulls and an equal sample of randomly selected unoccupied patches were compared with t‐tests. Based on presence–absence data from 1989 to 2006 we analysed factors affecting the probability of local colonization, extinction and the size of local populations using generalized linear models. Results Occupied habitat patches were significantly larger and less isolated (from other habitat patches and other local populations) and were located closer to rivers than empty patches. The proximity of local food resources (fish ponds, refuse dumps) positively affected the occurrence of breeding pairs. The probability of colonization was positively affected by patch area, and negatively by distances to fish ponds, nearest habitat patch, nearest breeding colony and to a river, and by higher forest cover around the patch boundaries. The probability of extinction was lower in patches with a higher number of breeding pairs and with a greater area of islets. The extinction probability increased with distances to other local populations, other habitat patches, fish ponds and to refuse dumps and with a higher cover of forest around the patch boundaries. The size of the local population decreased with distances to the nearest habitat patch, local population, river, fish pond and refuse dump. Local abundance was also positively affected by the area of islets in the patch. Main conclusions During the initial stages of the invasion of Caspian gulls in Poland the species underwent metapopulation‐like dynamics with frequent extinctions from colonized habitat patches. The results prove that metapopulation theory may be a useful conceptual framework for predicting which habitats are more vulnerable to invasion.     

Seasonal and spatial variation of the Montagu’s Harrier’s Circus pygargus diet in Eastern Poland

Capsule: The diet of the Montagu’s Harrier Circus pygargus is variable and comprises the most available prey at a given time of the season. We found no evidence for a relationship between diet and land-use in the core foraging zone.

Aims: We investigated whether the diet of the Montagu’s Harrier reflects the available prey and how it changes across the breeding season and in relation to land-use.

Methods: We analysed pellets collected at nests between 2007 and 2011. We looked for nonlinear patterns in the occurrence of prey categories in the pellets as a nonlinear function of the Julian date. Moreover, we tested whether the diet is affected by land-use within a radius of 2299 m from the nest.

Results: Four thousand four hundred and sixty-five prey items were found in 880 pellets and 76 prey remains collected at 63 sites. The diet did not depend on the land-use structure but showed a significant temporal variation.

Conclusions: The diet of the Montagu’s Harrier follows the availability of the prey in the foraging habitat. We conclude that the type of land-use in the vicinity of the nesting habitat has a rather weak effect on the diet of the Montagu’s Harrier.     

Lesser Black-backed Gulls Larus fuscus thriving on a non-marine diet

Capsule: Lesser Black-backed Gulls Larus fuscus breeding 30?km from the coast in the Netherlands focussed entirely on terrestrial food sources and reached relatively high breeding success.

Aim: To gain insight in the foraging ecology, habitat use and breeding performance of inland-breeding Lesser Black-backed Gulls.

Methods: We received data from seven birds fitted with global positioning system (GPS) loggers. The colony was frequently visited to collect pellets and boluses and to monitor reproductive success, mortality and growth rate of chicks.

Results: The GPS data revealed that mainly terrestrial habitats were used, 98% of these GPS positions were within 25?km of the colony. Refuse dumps were the most preferred sites, but also agricultural fields and freshwater bodies were often visited. Only two of the 710 recorded trips were directed to the North Sea. The pellet and bolus analyses confirmed the GPS data: no marine food remains were found. Breeding success of birds in the enclosure was relatively high, with 90% of eggs hatched and 51% of chicks fledged (1.6 chicks/pair).

Conclusions: Relying on terrestrial food is feasible when sources are available in the vicinity of the colony. We conclude that Lesser Black-backed Gulls could theoretically shift towards inland breeding after a fishery discards ban.     

Apparent survival rates of a long-lived partial migrant: the Yellow-legged Gull Larus michahellis

Capsule: In Yellow-legged Gull Larus michahellis populations from the eastern Adriatic Sea, there are considerable differences in the ratio of migratory birds and apparent survival among the colonies. Survival was dependent on age, colony and area of dispersal.

Aims: To identify the ratio of migratory birds within populations of Yellow-legged Gulls, and to compare apparent survival of gulls during migration in central and northern Europe and around the Adriatic Sea.

Methods: We analysed 15 years of resighting data of Yellow-legged Gulls using a MARK multi-state model to calculate survival rates. The effects of age, natal colony and area of dispersal were examined.

Results: Almost 60% of Adriatic Yellow-legged Gulls were migratory but the ratio varied among colonies (10.3–78.3%). Survival was dependent on age, colony and area of dispersal, with average values per group ranging between 0.599 (se 0.093) and 0.684 (se 0.084).

Conclusion: The ratio of migratory and dispersive Yellow-legged Gulls from different Adriatic colonies might be affected by both inheritance and food availability.     

Microbial Community Structure of Leaf-Cutter Ant Fungus Gardens and Refuse Dumps

Background

Leaf-cutter ants use fresh plant material to grow a mutualistic fungus that serves as the ants'' primary food source. Within fungus gardens, various plant compounds are metabolized and transformed into nutrients suitable for ant consumption. This symbiotic association produces a large amount of refuse consisting primarily of partly degraded plant material. A leaf-cutter ant colony is thus divided into two spatially and chemically distinct environments that together represent a plant biomass degradation gradient. Little is known about the microbial community structure in gardens and dumps or variation between lab and field colonies.

Methodology/Principal Findings

Using microbial membrane lipid analysis and a variety of community metrics, we assessed and compared the microbiota of fungus gardens and refuse dumps from both laboratory-maintained and field-collected colonies. We found that gardens contained a diverse and consistent community of microbes, dominated by Gram-negative bacteria, particularly γ-Proteobacteria and Bacteroidetes. These findings were consistent across lab and field gardens, as well as host ant taxa. In contrast, dumps were enriched for Gram-positive and anaerobic bacteria. Broad-scale clustering analyses revealed that community relatedness between samples reflected system component (gardens/dumps) rather than colony source (lab/field). At finer scales samples clustered according to colony source.

Conclusions/Significance

Here we report the first comparative analysis of the microbiota from leaf-cutter ant colonies. Our work reveals the presence of two distinct communities: one in the fungus garden and the other in the refuse dump. Though we find some effect of colony source on community structure, our data indicate the presence of consistently associated microbes within gardens and dumps. Substrate composition and system component appear to be the most important factor in structuring the microbial communities. These results thus suggest that resident communities are shaped by the plant degradation gradient created by ant behavior, specifically their fungiculture and waste management.     

DIET OF HARTLAUB'S GULL LARUS HARTLAUBU AND THE KELP GULL L. DOMINICANUS IN THE SOUTHWESTERN CAPE PROVINCE,SOUTH AFRICA

Steele, W.K. 1992. Diet of Hartlaub's Gull Larus hartlaubii and the Kelp Gull L. dominicanus in the southwestern Cape Province, South Africa. Ostrich 63:68-82.

Hartlaub's Larus hartlaubii and Kelp Gulls L. dominicanus in the southwestern Cape feed on a wide range of prey species. On average, Kelp Gulls feed on larger-sized prey than do the smaller Hartlaub's Gulls. In an undisturbed environment the preferred foraging habitats of Hartlaub's and Kelp Gulls are rocky shores and open, sandy beaches (where sand mussels Donax serra are common) respectively. However, as a result of man's activities, several new foraging habitats have become available including croplands, fishing harbours and refuse dumps. The provision of supplementary food at these new foraging habitats is likely to be the cause of a recent population increase. Kelp Gull chicks are fed predominantly “natural” prey which indicates that the population increase may not be due to enhanced reproductive success, but to improved post-fledging survival of juveniles, which are known to aggregate at sites where supplementary food is provided.