共查询到20条相似文献,搜索用时 31 毫秒
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Bassett CL Nickerson ML Farrell RE Harrison M 《Molecular genetics and genomics : MGG》2004,271(6):752-760
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de Boer Gert-Jan Testerink Christa Pielage Gerlof Nijkamp H. John J. Stuitje Antoine R. 《Plant molecular biology》1999,39(6):1197-1207
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Marc Drolet Luc Péloquin Yann Echelard Linda Cousineau A. Sasarman 《Molecular & general genetics : MGG》1989,216(2-3):347-352
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Masayoshi Yamaguchi Reiko Makino Noriaki Shimokawa 《Molecular and cellular biochemistry》1996,165(2):145-150
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The locations of the 3 ends of RNAs in rat ribosome were studied by a fluorescencelabeling method combined with high hydrostatic pressure and agarose electrophoresis. Under physiological conditions, only the 3 ends of 28 S and 5.8 S RNA in 80 S ribosome could be labeled with a high sensitive fluorescent probe – fluorescein 5thiosemicarbazide (FTSC), indicating that the 3 termini of 28 S and 5.8 S RNA were located on or near the surface of 80 S ribosome. The 3 terminus of 5 S RNA could be attacked by FTSC only in the case of the dissociation of the 80 S ribosome into two subunits induced by high salt concentration (1 M KCl) or at high hydrostatic pressure, showing that the 3 end of 5 S RNA was located on the interface of two subunits. However, no fluorescencelabeled 18 S RNA could be detected under all the conditions studied, suggesting that the 3 end of 18 S RNA was either located deeply inside ribosome or on the surface but protected by proteins. It was interesting to note that modifications of the 3 ends of ribosomal RNAs including oxidation with NaIO4, reduction with KBH4 and labeling with fluorescent probe did not destroy the translation activity of ribosome, indicating that the 3 ends of RNAs were not involved in the translation activity of ribosome. 相似文献
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