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1.
Three strains (LM008 T, LM068 and LM078 T), representing two novel yeast species were isolated from the phylloplane of three plant species by an enrichment technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and the sequence analysis of the D1/D2 domain of the large subunit rRNA gene and the internal spacer region, the three strains were assigned as two novel Candida species. Strain LM008 T was assigned to be Candida sirachaensis sp. nov. (type strain LM008 T = BCC 47628 T = NBRC 108605 T CBS 12094 T) in the Starmerella clade. Two strains (LM068 and LM078 T) represent a single species in the Lodderomyces- Spathaspora clade for which the name Candida sakaeoensis sp. nov. is proposed with the type strain LM078 T = BCC 47632 T = NBRC 108895 T = CBS 12318 T. 相似文献
2.
Five strains (LN12, LN14 T, LN15 T, LN16 and LN17 T) representing three novel methylotrophic yeast species were isolated from the external surface of plant leaves by three-consecutive
enrichments. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis
of the D1/D2 domain of the large subunit (LSU) rRNA gene and the phylogenetic analysis, the five strains were assigned to
be one novel Ogataea species and two novel Candida species. Three strains (LN12, LN14 T and LN16) represent a single novel species of the genus Ogataea, for which the name Ogataea phyllophila sp. nov. is proposed. The type strain is LN14 T (= BCC 42666 T = NBRC 107780 T = CBS 12095 T). Strain LN15 T was assigned to be Candida chumphonensis sp. nov. (type strain LN15 T = BCC 42667 T = NBRC 107781 T = CBS 12096 T). Strain LN17 T represented another novel species of Candida that was named Candida mattranensis sp. nov. (type strain LN17 T = BCC 42668 T = NBRC 107782 T = CBS 12097 T). 相似文献
4.
In a taxonomic study of yeasts isolated from decaying plant materials submerged in water of mangrove forests in Thailand,
three strains isolated from tree bark (EM33 T), a fallen leaf (EM40 T) and a detached branch (SM56 T) were found to represent three novel yeast species. On the basis of morphological, biochemical, physiological and chemotaxonomic
characteristics, the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, and the phylogenetic analysis,
the three strains were assigned as three novel Candida species. They were named as Candida chanthaburiensis sp. nov. (type strain EM33 T = BCC 23057 T = NBRC 102176 T = CBS 10926 T), Candida kungkrabaensis sp. nov. (type strain EM40 T = BCC 23060 T = NBRC 102179 T = CBS 10927 T), and Candida suratensis sp. nov. (type strain SM56 T = BCC 25961 T = NBRC 103858 T = CBS 10928 T). 相似文献
6.
Three hundred and thirty-seven xylose-utilizing yeast strains were isolated from various natural samples. Among these, 68 strains produced xylitol in the range of 0.1–0.69 g xylitol/g xylose. Thirty-nine xylitol-producing strains were identified to be Candida tropicalis. Ten strains were found belonging to 14 known species in the genus Candida, Cyberlindnera, Meyerozyma, Pichia, Wickerhamomyces, Yamadazyma and Cryptococcus. Two strains were identified to be two Candida species and two strains (DMKU-XE142 T and DMKU-XE332) were found to be a novel species. Strain DMKU-XE142 T was isolated from tree bark and DMKU-XE332 was obtained from decaying plant leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit rRNA gene (LSU) and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel Yamadazyma species although formation of ascospores was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and the ITS region of the two strains were identical but differed from Yamadazyma phyllophila, the closest species in terms of pairwise sequence similarity of the D1/D2 region, by 1.7 % nucleotide substitutions and 3.5 % nucleotide substitutions in the ITS region. The name Yamadazyma ubonensis f.a., sp. nov. is proposed (type strain is DMKU-XE142 T = BCC 61020 T = CBS 12859 T). 相似文献
7.
Four yeast strains were isolated from rotting wood samples collected from two sites in the Baotianman Nature Reserve and the Laojieling Nature Reserve in China. DNA sequence comparison and other taxonomic characteristics identified the strains as a single novel species of the genus Metschnikowia. The name Metschnikowia henanensis sp. nov. is proposed to accommodate these highly divergent organisms with the type strain BY-97 T (= CICC 1982 T = CBS 12677 T). The novel species produced chlamydospores, but it did not exhibit ascospore formation in sporulation media for 4 weeks. Molecular phylogeny from the D1/D2 domains of the large subunit (LSU) rRNA gene sequences placed this new species in a basal position to the Metschnikowia viticola/ Candida kofuensis/ Metschnikowia noctiluminum subclade, and an undescribed Candida species namely strains IMB-EMP4 and IMB-EMP5 was a close sister to M. henanensis. 相似文献
9.
During a survey of yeasts associated with raw honey collected in Thailand, two strains of the Zygoascus clade were isolated from the Asian cavity-nesting honeybee Apis cerana and the stingless bee Homotrigona fimbriata. Phylogeny based on 26S rDNA D1/D2 sequences placed these yeasts as members of a clade including Candida bituminiphila, Candida patagonica and Candida polysorbophila. The strains of the two novel species, CBS 12271 T and CBS 12270 T, respectively, could be unquestionably distinguished from their relatives by rDNA sequences and other taxonomic characteristics.
Therefore, the novel anamorphic species, Candida lundiana sp. nov. (type strain CBS 12271 T = JCM 16823 T) and Candida suthepensis sp. nov. (type strain CBS 12270 T = JCM 16822 T) are described. 相似文献
10.
Two newly isolated halotolerant obligately methylotrophic bacteria (strains C2 T and SK12 T) with the serine pathway of C 1 assimilation are described. The isolates are strictly aerobic, Gram negative, asporogenous, non-motile rods, forming rosettes, multiplying by binary fission. Mesophilic and neutrophilic, accumulate intracellularly compatible solute ectoine and poly-β-hydroxybutyrate. The novel strains are able to grow at 0 up to 16% NaCl (w/v), optimally at 3–5% NaCl. The major cellular fatty acids are C 18:1ω7c and C 19:0cyc and the prevailing quinone is Q-10. The predominant phospholipids are phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine and phosphatidylethanolamine. Assimilate NH 4+ by glutamate dehydrogenase and via the glutamate cycle (glutamine synthetase and glutamate synthase). The DNA G + C contents of strains C2 T and SK12 T are 60.9 and 60.5 mol% (Tm), respectively. 16S rRNA gene sequence similarity between the two new isolates are 99% but below 94% with other members of the Alphaproteobacteria thus indicating that they can be assigned to a novel genus Methyloligella. Rather low level of DNA–DNA relatedness (53%) between the strains C2 T and SK12 T indicates that they represent two separate species of the new genus, for which the names Methyloligella halotolerans gen. nov., sp. nov. and Methyloligella solikamskensis sp. nov. are proposed. The type strain of Methyloligella halotolerans is C2 T (=VKM B-2706 T = CCUG 61687 T = DSM 25045 T) and the type strain of Methyloligella solikamskensis is SK12 T (=VKM B-2707 T = CCUG 61697 T = DSM 25212 T). 相似文献
11.
Four yeast strains (APSS 805, APSS 806, APSS 815 and AP-18) belonging to a novel Torulaspora species were isolated from coal mine soils of Singareni in Andhra Pradesh state, India. Another strain (PBA-22) was isolated
from agricultural field soil from Gujarat state, India. The vegetative cells of all these strains were round, haploid and
produced asci by conjugation between independent cells or mother cell and bud, with rough ascospores, suggesting their possible
relation to ascomycetous yeast genus Torulaspora. Phylogenetic analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene and Internal Transcribed Spacer (ITS) regions
revealed that, among the five strains, three viz. APSS 805, APSS 806 and APSS 815 have identical sequences. The other two
strains (AP-18 and PBA-22) differed from the other three strains in less than 1% nucleotide substitutions in the combined
D1/D2 domain and ITS sequences, indicating that all of them (five strains) may belong to the same species. These five strains
were closely related to Torulaspora globosa, but showed more than 3–7% sequence divergence from T. globosa and all other species in the genus Torulaspora in the combined sequence analysis of D1/D2 domain and ITS region of rRNA gene. In addition, these strains also showed distinct
microsatellite finger-printing pattern from related species and differed in several physiological responses suggesting that
these strains belong to a novel species of Torulaspora. We propose to name these strains as Torulaspora indica sp. nov., and designate APSS 805 T = MTCC 9772 T = CBS 12408 T as the type strain of this novel species. The Mycobank number of the novel species is MB 563738. 相似文献
12.
Three strains (KM03 T, KM13 T and KM15) representing two novel methylotrophic yeast species were isolated from the external surface of plant leaves, which were collected from Kanchanaburi province, Thailand, by three-consecutive enrichments in methanol broth. Strain KM03 T was isolated from phylloplane of a mango tree ( Mangifera indica) and two strains, KM13 T and KM15, were obtained from phylloplane of different wine grapes ( Vitis vinifera). The sequences of the D1/D2 region of the large subunit (LSU) rRNA gene of the two strains (KM13 T and KM15) were identical and differed markedly from that of strain KM03 T. In terms of pairwise sequence similarity of the D1/D2 region the closest species to the strains KM13 T and KM15 were Candida suzukii (CBS 9253 T) and Candida nitratophila (CBS 2027 T) but with 2.1 % nucleotide substitutions. Strain KM03 T differed from Ogataea wickerhamii (CBS 4307 T), its closest relative, by 2.3 % nucleotide substitutions. Phylogenetic analysis based on the D1/D2 sequences placed the three strains in the Ogataea clade. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analyses of the D1/D2 and the internal transcribed spacer (ITS) regions of the nuclear ribosomal RNA gene (nrRNA) operon, the three strains represent two novel Ogataea species although formation of ascospores was not observed. Ogataea kanchanaburiensis sp. nov. is proposed for strain KM03 T (=BCC 47626 T = NBRC 108603 T = CBS 12673 T). Two strains, KM13 T and KM15, were assigned to Ogataea wangdongensis sp. nov. (type strain KM13 T = BCC 42664 T = NBRC 107778 T = CBS 12674 T). GenBank/EMBL/DDBJ accession numbers for the sequences of the D1/D2 and the ITS regions of O. kanchanaburiensis KM03 T are AB734090 and AB734093, respectively, of O. wangdongensis KM13 T are AB734091 and AB734094, respectively, and of O. wangdongensis KM15 are AB734092 and AB734095, respectively. 相似文献
13.
Four ascosporulating strains of an undescribed methanol-assimilating yeast species were isolated from forest habitats in Hungary.
Three were recovered from rotten wood and one from leaves of a sessile oak ( Quercus petraea). An additional isolate of the undescribed species sharing similar phenotypic characters with the above-noted strains was
recovered from the gut of an unidentified beetle collected from under the bark of a coniferous tree in Bulgaria. A closely
related, but somewhat divergent strain was recovered from insect frass in a Ponderosa pine ( Pinus ponderosa) collected in New Mexico, USA. Analysis of the D1/D2 sequences of the LSU rRNA gene placed the new species in the Ogataea clade. The ITS and the D1/D2 LSU sequences of the rRNA gene repeats were compared for the above-noted strains and that of
the type strain of Ogataea zsoltii, the closest neighbour among currently recognized Ogataea species. Their relatedness was investigated by parsimony network analysis as well. As a result of the sequence analysis,
it was concluded that the six strains isolated from tree associated habitats represent a single new yeast species. Ogataea saltuana sp. nov. is proposed to accommodate these strains. The type strain NCAIM Y.01833 T (CBS 10795 T, NRRL Y-48448 T) was recovered from rotten wood of Scotch pine ( Pinus silvestris) in Hungary. The GenBank accession number for the D1/D2 domain nuclear large subunit rRNA gene sequence of strain NCAIM Y.01833 T (CBS 10795 T, NRRL Y-48448 T) is EU327033. The MycoBank number of the new species is MB 519966. 相似文献
14.
Four strains representing three novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (DMKU-RK254 T), corn leaves (DMKU-RK548 T), bean leaves (K129) in Thailand and hengchun pencilwood leaves (TrB1-1 T) in Taiwan. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region, the actin gene (ACT1) and the elongation factor 2 gene (EF2), the four strains were determined to represent novel Yamadazyma species although formation of ascospores was not observed. Strain DMKU-RK254 T was determined to be related to Candida diddensiae, Candida naeodendra and Candida kanchanaburiensis but with 1.8, 1.8 and 2.0 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, respectively. It was assigned to Yamadazyma siamensis sp. nov. (type strain DMKU-RK254 T = BCC 50730 T = NBRC 108901 T = CBS 12573 T). The sequences of the D1/D2 region of the LSU rRNA gene, the ITS region, ACT1 gene and EF2 gene of two strains (DMKU-RK548 T and K129) were identical but differed from that of strain TrB1-1 T by 0.6, 1.0, 3.3 and 5.9 % nucleotide substitutions, respectively. Therefore, the two strains (DMKU-RK548 T and K129) and strain TrB1-1 T were assigned to be two separate species. The closest species in terms of pairwise sequences similarity of the D1/D2 region to the two novel species was Yamadazyma philogaea but with 1.1–1.7 % nucleotide substitutions. The two strains (DMKU-RK548 T and K129) were assigned to Yamadazyma phyllophila sp. nov. (type strain DMKU-RK548 T = BCC 50736 T = NBRC 108906 T = CBS 12572 T) and the strain TrB1-1 T was named Yamadazyma paraphyllophila sp. nov. (type strain TrB1-1 T = BCRC 23030 T = CCTCC AY 204005 T = CBS 9928 T). 相似文献
15.
Twelve strains representing five novel yeast species were isolated from natural samples distributed in mountain areas in Taiwan during 2007 and 2009. Sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene revealed that these species are members of the Cyberlindnera clade. These five new species have a greater than 1% difference from their closest relatives in the sequences of the D1/D2 domain of the LSU rRNA gene and were well separated from their closest relatives in terms of physiological characteristics. Moreover, a sexual state could not be found in these five novel yeast species. Therefore, the scientific names of Candida maesa sp. nov. (type strain GJ8L01 T), Candida takata sp. nov. (type strain EN25S01 T), Candida taoyuanica sp. nov. (type strain GY15S07 T), Candida hungchunana sp. nov. (type strain NC3W71 T) and Candida stauntonica sp. nov. (type strain GY13L05 T) were proposed to accommodate these yeasts. 相似文献
16.
Parsimony network analysis of rDNA sequences was used to delimit phylogenetic species of yeasts in an objective, formal manner.
Many strains assigned to Candida apicola ( Starmerella clade), when compared to the type, fell outside the inclusion limits proposed by Kurtzman and Robnett ( 1998) based on a pair-wise comparison of the large subunit rRNA gene D1/D2 domains. However, when these sequences were analyzed
jointly with ITS rDNA sequences by parsimony network analysis, 28 of the 30 strains formed a cohesive set. Two strains, MUCL
45721 and CBS 4353, were excluded from the species, but there was no evident justification to subdivide the rest. A similar
analysis of 81 isolates originally assigned to Candida azyma ( Wickerhamiella clade) yielded dramatically different results, giving rise to six independent networks corresponding to Candida azyma sensu stricto (18 strains), Candida azymoides (2 strains), a pair of isolates from Australian hibiscus flowers, a single isolate from the same substrate, a single isolate
from Malaysian bertam palm nectar, and 57 isolates that are assigned to the new species Candida parazyma (type = UWOPS 91-652.1 T = CBS 11563 T = NRRL Y-48669 T). The strains retained in C. azyma sensu stricto differed from one another by up to four substitutions in their D1/D2 sequences, but their polymorphism at the
level of the ITS was considerable and suggested a history of divergence resulting from dispersal. Strains of C. parazyma fell into seven variant haplotypes based on sequences of the rDNA ITS and D1/D2 regions. The most abundant haplotype occurred
across the global range of the species. Others were either endemic to Belize, Costa Rica, Rarotonga, or Tennessee, suggestive
of vicariance, or occurred across remote localities, offering partial support to the notion of rapid dispersal. 相似文献
17.
Three ascosporogenous yeast strains were isolated from the gut of the passalid beetle, Odontotaenius disjunctus, inhabiting on rotten oak trees. DNA sequence comparison and other taxonomic characteristics identified the strains as a
novel species in the genus Kazachstania. The name Kazachstania
intestinalis sp. nov. (type strain EH085 T = ATCC MYA-4658 T = CBS 11839 T) is proposed for the strains. The yeast is homothallic, producing persistent asci with 1–4 spheroidal ascospores. Molecular
phylogeny from ribosomal RNA gene sequences placed this novel species on the basal lineage of a clade including Kazachstania lodderae, Kazachstania exigua, Kazachstania martiniae, and other related Kazachstania spp., but none of those species was a close sister to K.
intestinalis. 相似文献
18.
PurposeThe aim of this study was to prove that Terrilactibacillus laevilacticus SK5-6, a novel D-lactate producer, exhibited a good fermentation performance comparing to the reference D-lactate producer Sporolactobacillus sp. MethodsGlucose bioconversion for D-lactate production and the activity of five key enzymes including phosphofructokinase (PFK), pyruvate kinase (PYK), D-lactate dehydrogenase (D-LDH), L-lactate dehydrogenase (L-LDH), and lactate isomerase (LI) were investigated in the cultivation of T. laevilacticus SK5–6 and S. laevolacticus 0361T. ResultsT. laevilacticus SK5–6 produced D-lactate at higher yield, productivity, and optical purity compared with S. laevolacticus 0361T. T. laevilacticus SK5–6, the catalase-positive isolate, simultaneously grew and produced D-lactate without lag phase while delayed growth and D-lactate production were observed in the culture of S. laevolacticus 0361T. The higher production of D-lactate in T. laevilacticus SK5–6 was due to the higher growth rate and the higher specific activities of the key enzymes observed at the early stage of the fermentation. The low isomerization activity was responsible for the high optical purity of D-lactate in the cultivation of T. laevilacticus SK5–6. ConclusionThe lowest specific activity of PFK following by PYK and D/L-LDHs, respectively, indicated that the conversion of fructose-6-phosphate was the rate limiting step. Under the well-optimized conditions, the activation of D/L-LDHs by fructose-1,6-phosphate and ATP regeneration by PYK drove glucose bioconversion toward D-lactate. The optical purity of D-lactate was controlled by D/L-LDHs and the activation of isomerases. High D-LDH with limited isomerase activity was preferable during the fermentation as it assured the high optical purity. 相似文献
19.
Brown rot caused by Monilinia spp. is among the most important postharvest diseases of commercially grown stone fruits, and application of antagonistic yeasts to control brown rot is one promising strategy alternative to chemical fungicides. In this research, new yeast strains were isolated and tested for their activity against peach brown rot caused by Monilinia fructicola. Three yeast strains were originally isolated from the surface of plums (cv Chinese Angelino) collected in the north of China. In artificially wounded inoculation tests, the yeast reduced the brown rot incidence to 20 %. The population of the yeast within inoculated wounds on peaches significantly increased at 25 °C from an initial level of 5.0 × 10 6 to 4.45 × 10 7 CFU per wound after 1 day. The antagonistic strains were belonging to a new species of the genus Candida by sequence comparisons of 26 S rDNA D1/D2 domain and internal transcribed spacer region. The strains are most closely related to C. asparagi, C. musae and C. fructus on the basis of the phylogenetic trees based on the D1/D2 region of 26S rDNA. However, the strains are notably different from C. asparagi, C. musae and C. fructus, in morphological and physiological characteristics. Therefore, the name Candida pruni is proposed for the novel species, with sp-Quan (=CBS12814 T = KCTC 27526 T = GCMC 6582 T) as the type strain. Our study showed that Candida pruni is a novel yeast species with potential biocontrol against brown rot caused by M. fructicola on peaches. 相似文献
20.
In this study, sStrain SK‐1T, a novel gram‐positive, pleomorphic, rod‐shaped, non‐spore forming, non‐motile organism, designated SK‐1T, was isolated from human gingival sulcus and found to produce acetic acid, propionic acid, lactic acid, and succinic acid as end products of glucose fermentation. Strain SK‐1T is most closely related to Pseudopropionibacterium (Propionibacterium) propionicum with sequence homologies of the 16S rRNA and RNA polymerase β subunit (rpoB) genes of 96.6% and 93.1%, respectively. The genomic DNA G + C content of the isolate was 61.8 mol%. On the basis of the sequence data of the 16S rRNA and housekeeping (rpoB) genes, a novel taxon is here proposed, Pseudopropionibacterium rubrum sp. nov. (type strain SK‐1T = JCM 31317T = DSM 100122T). The 16S rRNA and rpoB gene sequences of strain SK‐1T have been deposited in the DDBJ under the accession numbers LC002971 and LC102236, respectively. 相似文献
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