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1.
Stojałowski S Myśków B Milczarski P Masojć P 《Cellular & molecular biology letters》2009,14(2):190-198
Four F2 mapping populations derived from crosses between rye inbred lines DS2×RXL10, 541×Ot1-3, S120×S76 and 544×Ot0-20 were used
to develop a consensus map of chromosome 6R. Thirteen marker loci that were polymorphic in more than one mapping population
constituted the basis for the alignment of the four maps using the JoinMap v. 3.0 software package. The consensus map consists
of 104 molecular marker loci including RFLPs, RAPDs, AFLPs, SSRs, ISSRs, SCARs, STSs and isozymes. The average distance between
the marker loci is 1.3 cM, and the total map length is 135.5 cM. This consensus map may be used as a source of molecular markers
for the rapid development of new maps of chromosome 6R in any mapping population. 相似文献
2.
J. B. Clarke D. J. Sargent R. I. Bošković A. Belaj K. R. Tobutt 《Tree Genetics & Genomes》2009,5(1):41-51
One hundred and sixty microsatellite (simple sequence repeat (SSR)) and six gene-specific markers revealing 174 loci were
scored in 94 seedlings from the inter-specific cross of Prunus avium ‘Napoleon’ × Prunus nipponica accession F1292. The co-segregation data from these markers were used to construct a linkage map for cherry which spanned
680 cM over eight linkage groups with an average marker spacing of 3.9 cM per marker and just six gaps longer than 15 cM.
Markers previously mapped in Prunus dulcis ‘Texas’ × Prunus persica ‘Earlygold’ allowed the cherry map to be anchored to the peach × almond map and showed the high level of synteny between
the species. Eighty-four loci segregated in P. avium ‘Napoleon’ versus 159 in P. nipponica. The segregations of 32 isoenzyme loci in a subset of 47 seedlings from the progeny were scored, using polyacrylamide gel
electrophoresis and/or isoelectric focusing separation followed by activity staining, and the co-segregation data were analysed
along with those for 39 isoenzymes reported previously and for the 174 sequence-tagged site loci plus an additional two SSR
loci. The second map incorporates 233 loci and spans 736 cM over eight linkage groups with an average marker spacing of 3.2 cM
per marker and just two gaps greater than 15 cM. The microsatellite map will provide a useful tool for cherry breeding and
marker-assisted selection and for synteny studies within Prunus; the gene-specific markers and isoenzymes will be useful for comparisons with maps of other rosaceous fruit crops.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
3.
J. M. Soriano E. M. Vera-Ruiz S. Vilanova J. Martínez-Calvo G. Llácer M. L. Badenes C. Romero 《Tree Genetics & Genomes》2008,4(3):391-402
Sharka disease, caused by the plum pox virus (PPV), is one of the major limiting factors for stone fruit crops in Europe and
America. In particular, apricot is severely affected suffering significant fruit losses. Thus, PPV resistance is a trait of
great interest for the apricot breeding programs currently in progress. In this work, two apricot maps, earlier constructed
with the F1 ‘Goldrich × Currot’ (G×C) and the F2 ‘Lito × Lito’-98 (L×L-98) populations, have been improved including 43 and 37 new simple sequence repeat (SSR) loci, respectively,
to facilitate PPV resistance trait mapping. Screening of PPV resistance on the segregating populations classified seedling
phenotypes into resistant or susceptible. A non-parametric mapping method, based on the Kruskal–Wallis (KW) rank sum test,
was initially used to score marker–trait association, and results were confirmed by interval mapping. Contrary to the putative
digenic model inferred from the phenotypic segregations, all significant markers for the KW statistic (P < 0.005) mapped in a unique region of ~21.0 and ~20.3 cM located on the upper part of the G1 linkage group in ‘G×C’ and ‘L×L-98’
maps, respectively. According to the data, PPV resistance is suggested to be controlled by at least one major dominant locus.
The association between three SSRs distributed within this region and the PPV resistance was tested in two additional populations
(‘Goldrich × Canino’ and ‘Lito × Lito’-00) and breeding program parents. The marker ssrPaCITA5 showed the highest KW value
(P < 0.005) in all cases, pointing out its usefulness in marker-assisted selection.
Electronic Supplementary Material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
4.
Marker-assisted selection (MAS) offers quick and reliable prediction of the phenotypes of seedlings in large populations and
thus opens new approaches for selection to breeders of apple (Malus x domestica Borkh.). The development of framework maps enables the discovery of genetic markers linked to desired traits. Although genetic
maps have been reported for apple scion cultivars, none has previously been constructed for apple rootstocks. We report the
construction of framework genetic maps in a cross between ‘M.9’ (‘Malling 9’) and ‘R.5’ (‘Robusta 5’) apple rootstocks. The
maps comprise 224 simple sequence repeat (SSR) markers, 18 sequence-characterised amplified regions, 14 single nucleotide
polymorphisms and 42 random amplified polymorphic DNAs. A new set of 47 polymorphic SSRs was developed from apple EST sequences
and used for construction of this rootstock map. All 17 linkage groups have been identified and aligned to existing apple
genetic maps. The maps span 1,175.7 cM (‘M.9’) and 1,086.7 cM (‘R.5’). To improve the efficiency of mapping markers to this
framework map, we developed a bin mapping set. Applications of these new genetic maps include the elucidation of the genetic
basis of the dwarfing effect of the apple rootstock ‘M.9’ and the analysis of disease and insect resistance traits such as
fire blight (Erwinia amylovora), apple scab (Venturia inaequalis) and woolly apple aphid (Eriosoma lanigerum). Markers for traits mapped in this population will be of direct use to apple breeders for MAS and for identification of
causative genes by map-based cloning. 相似文献
5.
Megumi Igarashi Yoshie Abe Yoshimichi Hatsuyama Takanori Ueda Tomoko Fukasawa-Akada Tomoyuki Kon Tsuyoshi Kudo Takashi Sato Masahiko Suzuki 《Molecular breeding : new strategies in plant improvement》2008,22(1):95-118
Two apple genetic linkage maps were constructed using amplified fragment length polymorphisms (AFLPs), simple sequence repeats
(SSRs), random amplified polymorphic DNAs (RAPDs), and expressed sequence tag (EST)-derived markers in combination with a
pseudo-testcross mapping strategy in which the cultivars ‘Ralls Janet’ and ‘Delicious’ were used as the respective seed parents.
Mitsubakaido (Malus sieboldii) was used as the pollen parent for each of the segregating F1 populations. Expressed sequence tag data were obtained from the random sequencing of cDNA libraries constructed from in vitro
cultured shoots and maturing fruits of cv ‘Fuji’, which is the offspring of a cross between ‘Ralls Janet’ and ‘Delicious’.
In addition, a number of published gene sequences were used to develop markers for mapping. The ‘Ralls Janet’ map consisted
of 346 markers (178 AFLPs, 95 RAPDs, 54 SSRs, 18 ESTs, and the S locus) in 17 linkage groups, with a total length of 1082 cM, while that of ‘Delicious’ comprised 300 markers (120 AFLPs,
81 RAPDs, 64 SSRs, 32 ESTs, and the S, Rf, and MdACS-1 loci) on 17 linkage groups spanning 1031 cM. These maps are amenable to comparisons with previously published maps of ‘Fiesta’
and ‘Discovery’ (Liebhard et al., Mol Breed 10:217–241, 2002; Liebhard et al., Theor Appl Genet 106:1497–1508, 2003a) because several of the SSRs (one to three markers per linkage group) were used in all of the maps. Distorted marker segregation
was observed in three and two regions of the ‘Ralls Janet’ and ‘Delicious’ maps, respectively. These regions were localized
in different parts of the genome from those in previously reported apple linkage maps. This marker distortion may be dependent
on the combinations of cultivars used for map construction. 相似文献
6.
Molecular linkage mapping in rye (Secale cereale L.) 总被引:3,自引:0,他引:3
X.-F. Ma M. K. Wanous K. Houchins M. A. Rodriguez Milla P. G. Goicoechea Z. Wang M. Xie J. P. Gustafson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):517-523
A rye linkage map containing clones from rye, wheat, barley, oat and rice genomic and cDNA libraries, known-function genes
and microsatellite markers, was created using an F2 population consisting of 110 F2-derived F3 families. Both co-dominant and dominant markers were added to the map. Of all probes screened, 30.8% were polymorphic, and
of those polymorphic 79.3% were mapped. The current map contains 184 markers present in all seven linkage groups covering
only 727.3 cM. This places a marker about every 3.96 cM on average throughout the map; however, large gaps are still present.
The map contains 60 markers that have been integrated from previous rye maps. Surprisingly, no markers were placed between
the centromere and C1–1RS in the short arm of 1R. The short arm of chromosome 4 also lacked an adequate number of polymorphic
markers. The population showed a remarkable degree of segregation distortion (72.8%). In addition, the genetic distance observed
in rye was found to be very different among the maps created by different mapping populations.
Received: 10 January 2000 / Accepted: 26 May 2000 相似文献
7.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
8.
Sargent DJ Passey T Surbanovski N Lopez Girona E Kuchta P Davik J Harrison R Passey A Whitehouse AB Simpson DW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(7):1229-1240
The linkage maps of the cultivated strawberry, Fragaria × ananassa (2n = 8x = 56) that have been reported to date have been developed predominantly from AFLPs, along with supplementation with transferrable
microsatellite (SSR) markers. For the investigation of the inheritance of morphological characters in the cultivated strawberry
and for the development of tools for marker-assisted breeding and selection, it is desirable to populate maps of the genome
with an abundance of transferrable molecular markers such as microsatellites (SSRs) and gene-specific markers. Exploiting
the recent release of the genome sequence of the diploid F. vesca, and the publication of an extensive number of polymorphic SSR markers for the genus Fragaria, we have extended the linkage map of the ‘Redgauntlet’ × ‘Hapil’ (RG × H) mapping population to include a further 330 loci,
generated from 160 primer pairs, to create a linkage map for F. × ananassa containing 549 loci, 490 of which are transferrable SSR or gene-specific markers. The map covers 2140.3 cM in the expected
28 linkage groups for an integrated map (where one group is composed of two separate male and female maps), which represents
an estimated 91% of the cultivated strawberry genome. Despite the relative saturation of the linkage map on the majority of
linkage groups, regions of apparent extensive homozygosity were identified in the genomes of ‘Redgauntlet’ and ‘Hapil’ which
may be indicative of allele fixation during the breeding and selection of modern F. × ananassa cultivars. The genomes of the octoploid and diploid Fragaria are largely collinear, but through comparison of mapped markers on the RG × H linkage map to their positions on the genome
sequence of F. vesca, a number of inversions were identified that may have occurred before the polyploidisation event that led to the evolution
of the modern octoploid strawberry species. 相似文献
9.
Construction of an integrated consensus map of the apple genome based on four mapping populations 总被引:2,自引:0,他引:2
A. N’Diaye W. E. Van de Weg L. P. Kodde B. Koller F. Dunemann M. Thiermann S. Tartarini F. Gennari C. E. Durel 《Tree Genetics & Genomes》2008,4(4):727-743
An integrated consensus genetic map for apple was constructed on the basis of segregation data from four genetically connected crosses (C1?=?Discovery × TN10-8, C2?=?Fiesta × Discovery, C3?=?Discovery × Prima, C4?=?Durello di Forli × Fiesta) with a total of 676 individuals using CarthaGene® software. First, integrated female–male maps were built for each population using common female–male simple sequence repeat markers (SSRs). Then, common SSRs over populations were used for the consensus map integration. The integrated consensus map consists of 1,046 markers, of which 159 are SSR markers, distributed over 17 linkage groups reflecting the basic chromosome number of apple. The total length of the integrated consensus map was 1,032 cM with a mean distance between adjacent loci of 1.1 cM. Markers were proportionally distributed over the 17 linkage groups (χ 2?=?16.53, df?=?16, p?=?0.41). A non-uniform marker distribution was observed within all of the linkage groups (LGs). Clustering of markers at the same position (within a 1-cM window) was observed throughout LGs and consisted predominantly of only two to three linked markers. The four integrated female–male maps showed a very good colinearity in marker order for their common markers, except for only two (CH01h01, CH05g03) and three (CH05a02z, NZ02b01, Lap-1) markers on LG17 and LG15, respectively. This integrated consensus map provides a framework for performing quantitative trait locus (QTL) detection in a multi-population design and evaluating the genetic background effect on QTL expression. 相似文献
10.
Zhigang Wei Kaixuan Zhang Chuanping Yang Guifeng Liu Guanjun Liu Lian Lian Hanguo Zhang 《Plant Molecular Biology Reporter》2010,28(1):169-175
Two separate genetic linkage maps for Chinese silver birch based on inter-simple sequence repeat (ISSR) and amplified fragment-length
polymorphism (AFLP) were constructed by a pseudo-testcross mapping strategy. Eighty F1 progenies were obtained from the cross between two parental trees with desirable traits (the paternal one selected from ‘Qinghai’
and the maternal one from ‘Wangqing’). A total of 46 ISSR primers and 31 AFLP primers were employed to generate 102 ISSR and
355 AFLP polymorphic markers in the F1 progenies. About 5.7% of all the markers displayed high segregation distortion with a P value below 0.01 and such markers were not used for map constructions. The paternal map consisted of 137 loci, spread over
13 groups and spanned 694.2 cM at an average distance of 5.1 cM between the markers, while in the maternal map, 147 loci were
distributed in 14 groups covering a map distance about 949.62 cM at an average distance of 6.5 cM. These initial maps can
serve as the basis for developing a more detailed genetic map. 相似文献
11.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献
12.
Sibylle Stoeckli Karsten Mody Andrea Patocchi Markus Kellerhals Silvia Dorn 《Tree Genetics & Genomes》2009,5(1):257-267
The aim of this study was to assess the genetic basis of rust mite (Aculus schlechtendali) resistance in apple (Malus × domestica). A. schlechtendali infestation of apple trees has increased as a consequence of reduced side effects of modern fungicides on rust mites. An
analysis of quantitative trait loci (QTLs) was carried out using linkage map data available for F1 progeny plants of the cultivars ‘Fiesta’ × ‘Discovery’. Apple trees representing 160 different genotypes were surveyed for
rust mite infestation, each at three different sites in two consecutive years. The distribution of rust mites on the individual
apple genotypes was aggregated and significantly affected by apple genotype and site. We identified two QTLs for A. schlechtendali resistance on linkage group 7 of ‘Fiesta’. The AFLP marker E35M42-0146 (20.2 cM) and the RAPD marker AE10-400 (45.8 cM) were
closest positioned to the QTLs and explained between 11.0% and 16.6% of the phenotypic variability. Additionally, putative
QTLs on the ‘Discovery’ chromosomes 4, 5 and 8 were detected. The SSR marker Hi03a10 identified to be associated to one of
the QTLs (AFLP marker E35M42-0146) was traced back in the ‘Fiesta’ pedigree to the apple cultivar ‘Wagener’. This marker may
facilitate the breeding of resistant apple cultivars by marker assisted selection. Furthermore, the genetic background of
rust mite resistance in existing cultivars can be evaluated by testing them for the identified SSR marker.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Tenhola-Roininen T Kalendar R Schulman AH Tanhuanpää P 《Journal of applied genetics》2011,52(3):299-304
A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through
anther culture of F1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289
loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite
amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), inter-simple sequence repeat (ISSR) and
sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of
the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P ≤ 0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found,
which explained 16.1% of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74,
RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease
sprouting damage. 相似文献
14.
Di Gaspero G Cipriani G Adam-Blondon AF Testolin R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(7):1249-1263
Genetic maps functionally oriented towards disease resistance have been constructed in grapevine by analysing with a simultaneous
maximum-likelihood estimation of linkage 502 markers including microsatellites and resistance gene analogs (RGAs). Mapping
material consisted of two pseudo-testcrosses, ‘Chardonnay’ × ‘Bianca’ and ‘Cabernet Sauvignon’ × ‘20/3’ where the seed parents
were Vitis vinifera genotypes and the male parents were Vitis hybrids carrying resistance to mildew diseases. Individual maps included 320–364 markers each. The simultaneous use of two
mapping crosses made with two pairs of distantly related parents allowed mapping as much as 91% of the markers tested. The
integrated map included 420 Simple Sequence Repeat (SSR) markers that identified 536 SSR loci and 82 RGA markers that identified
173 RGA loci. This map consisted of 19 linkage groups (LGs) corresponding to the grape haploid chromosome number, had a total
length of 1,676 cM and a mean distance between adjacent loci of 3.6 cM. Single-locus SSR markers were randomly distributed
over the map (CD = 1.12). RGA markers were found in 18 of the 19 LGs but most of them (83%) were clustered on seven LGs, namely
groups 3, 7, 9, 12, 13, 18 and 19. Several RGA clusters mapped to chromosomal regions where phenotypic traits of resistance
to fungal diseases such as downy mildew and powdery mildew, bacterial diseases such as Pierce’s disease, and pests such as
dagger and root-knot nematode, were previously mapped in different segregating populations. The high number of RGA markers
integrated into this new map will help find markers linked to genetic determinants of different pest and disease resistances
in grape.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
V. G. M. Bus D. Chagné H. C. M. Bassett D. Bowatte F. Calenge J.-M. Celton C.-E. Durel M. T. Malone A. Patocchi A. C. Ranatunga E. H. A. Rikkerink D. S. Tustin J. Zhou S. E. Gardiner 《Tree Genetics & Genomes》2008,4(2):223-236
Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) can be a major economic problem to apple growers in most parts of the world, and resistance breeding provides a sustainable
means to control this pest. We report molecular markers for three genes conferring WAA resistance and placing them on two
linkage groups (LG) on the genetic map of apple. The Er1 and Er2 genes derived from ‘Northern Spy’ and ‘Robusta 5,’ respectively, are the two major genes that breeders have used to date
to improve the resistance of apple rootstocks to this pest. The gene Er3, from ‘Aotea 1’ (an accession classified as Malus sieboldii), is a new major gene for WAA resistance. Genetic markers linked to the Er1 and Er3 genes were identified by screening random amplification of polymorphic deoxyribonucleic acid (DNA; RAPD) markers across DNA
bulks from resistant and susceptible plants from populations segregating for these genes. The closest RAPD markers were converted
into sequence-characterized amplified region markers and the genome location of these two genes was assigned to LG 08 by aligning
the maps around the genes with a reference map of ‘Discovery’ using microsatellite markers. The Er2 gene was located on LG 17 of ‘Robusta 5’ using a genetic map developed in a M.9 × ‘Robusta 5’ progeny. Markers for each of
the genes were validated for their usefulness for marker-assisted selection in separate populations. The potential use of
the genetic markers for these genes in the breeding of apple cultivars with durable resistance to WAA is discussed. 相似文献
16.
F. Fernández-Fernández K. M. Evans J. B. Clarke C. L. Govan C. M. James S. Marić K. R. Tobutt 《Tree Genetics & Genomes》2008,4(3):587-479
Simple sequence repeat (SSR) markers developed from Malus, as well as Prunus, Pyrus and Sorbus, and some other sequence-tagged site (STS) loci were analysed in an interspecific F1 apple progeny from the cross ‘Fiesta’ × ‘Totem’ that segregated for several agronomic characters. A linkage map was constructed
using 259 STS loci (247 SSRs, four SCARs and eight known-function genes) and five genes for agronomic traits—scab resistance
(Vf), mildew resistance (Pl-2), columnar growth habit (Co), red tissues (Rt) and green flesh background colour (Gfc). Ninety SSR loci and three genes (ETR1, Rt and Gfc) were mapped for the first time in apple. The transferability of markers from other Maloideae to Malus was found to be around 44%. The loci are spread across 17 linkage groups, corresponding to the basic chromosome number of
Malus and cover 1,208 cM, approximately 85% of the estimated length of the apple genome. Interestingly, we have extended the top
of LG15 with eight markers covering 25 cM. The average map density is 4.7 cM per marker; however, marker density varies greatly
between linkage groups, from 2.5 in LG14 to 8.9 in LG7, with some areas of the genome still in need of further STS markers
for saturation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
An erratum to this article can be found at 相似文献
17.
Analysis of RFLP mapping inaccuracy in Brassica napus L. 总被引:3,自引:0,他引:3
S. Cloutier M. Cappadocia B. S. Landry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):83-91
We identified sources of mapping inaccuracy during the construction of RFLP linkage maps from one F2 population and two F1 microspore-derived populations from the same cross of oilseed Brassica napus. The genetic maps were compared using a total of 145 RFLP marker loci including 82 loci common to all three populations.
In the process, we identified a series of mapping events that could lead to ambigous conclusions. Superimposed restriction
fragments could be mistaken as a single dominant restriction fragment in a F2 population and, when analyzed as such, would yield inaccurate linkage information. Residual heterozygosity in parental lines
resulted in complicated allelic assignment and yielded subsequent difficulties in linkage determination. Loose and spurious
linkages occurred during mapping and were identified by comparing maps derived from different populations. LOD scores and
χ2 test of independence were compared for their capacity to detect loose linkages or generate spurious ones. Extreme segregation
distortions towards the same parental allele also contributed to an additional source of spurious linkage. Small but significant
segregation distortions resulted in reduced estimates of the recombination fraction. The use of the same ‘probe× enzyme’ combinations
in doubled haploid populations allowed the identification of the correct allele assignment as well as loose and spurious linkages.
A translocation between two homoeologous linkage groups was observed. The consequences of such a chromosomal event as a source
of error in mapping applications are discussed.
Received: 7 September 1996/Accepted: 25 October 1996 相似文献
18.
Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers 总被引:1,自引:0,他引:1
Jim C. Cervantes-Flores G. Craig Yencho Albert Kriegner Kenneth V. Pecota Maria A. Faulk Robert O. M. Mwanga Bryon R. Sosinski 《Molecular breeding : new strategies in plant improvement》2008,21(4):511-532
Sweetpotato genomic research is minimal compared to most other major crops despite its worldwide importance as a food crop.
The development of a genetic linkage map in sweetpotato will provide valuable information about the genomic organization of
this important species that can be used by breeders to accelerate the introgression of desired traits into breeding lines.
We developed a mapping population consisting of 240 individuals of a cross between ‘Tanzania’, a cream-fleshed African landrace,
and ‘Beauregard’, an orange-fleshed US sweetpotato cultivar. The genetic linkage map of this population was constructed using
Amplified Fragment Length Polymorphism (AFLP) markers. A total of 1944 (‘Tanzania’) and 1751 (‘Beauregard’) AFLP markers,
of which 1511 and 1303 were single-dose markers respectively, were scored. Framework maps consisting of 86 and 90 linkage
groups for ‘Tanzania’ and ‘Beauregard’ respectively, were developed using a combination of JoinMap 3.0 and MAPMAKER/EXP 3.0.
A total of 947 single-dose markers were placed in the final framework linkage map for ‘Tanzania’. The linkage map size was
estimated as 5792 cM, with an average distance between markers of 4.5 cM. A total of 726 single-dose markers were placed in
the final framework map for ‘Beauregard’. The linkage map length was estimated as 5276 cM, with an average distance between
markers of 4.8 cM. Duplex and triple-dose markers were used to identify the corresponding homologous groups in the maps. Our
research supports the hypothesis that sweetpotato is an autopolyploid. Distorted segregation in some markers of different
dosages in this study suggests that some preferential pairing occurs in sweetpotato. However, strict allopolyploid inheritance
in sweetpotato can be ruled out due to the observed segregation ratios of the markers, and the proportion of simplex to multiple-dose
markers.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
This paper is a portion of a dissertation submitted by Jim C. Cervantes-Flores. 相似文献
19.
Márta Molnár-Láng András Cseh Éva Szakács István Molnár 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(8):1535-1545
The main objective of the present work was to develop a wheat genotype containing both the recessive crossability alleles
(kr1kr1kr2kr2), allowing high crossability between 6x wheat and diploid rye, and the 1BL.1RS wheat/rye translocation chromosome. This wheat
genotype could be used as a recipient partner in wheat–rye crosses for the efficient introduction of new allelic variation
into 1RS in translocation wheats. After crossing the wheat cultivars ‘Mv Magdaléna’ and ‘Mv Béres’, which carry the 1BL.1RS
translocation involving the 1RS chromosome arm from ‘Petkus’, with the line ‘Mv9 kr1’, 117 F2 plants were analysed for crossability, ten of which had higher than 50% seed set with rye and thus presumably carried the
kr1kr1kr2kr2 alleles. Four of the ten plants contained the 1BL.1RS translocation in the disomic condition as detected by genomic in situ
hybridization (GISH). The wheat × rye F1 hybrids produced between these lines and the rye cultivar ‘Kriszta’ were analysed in meiosis using GISH. 1BL.1RS/1R chromosome
pairing was detected in 62.4% of the pollen mother cells. The use of fluorescent in situ hybridization (FISH) with the repetitive
DNA probes pSc119.2, Afa family and pTa71 allowed the 1R and 1BL.1RS chromosomes to be identified. The presence of the 1RS
arm from ‘Kriszta’ besides that of ‘Petkus’ was demonstrated in the F1 hybrids using the rye SSR markers RMS13 and SCM9. In four of the 22 BC1 progenies analysed, only ‘Kriszta’-specific bands were observed with these markers, though the presence of the 1BL.1RS translocation
was detected using GISH. It can be concluded that recombination occurred between the ‘Petkus’ and ‘Kriszta’ 1RS chromosome
arms in the translocated chromosome in these plants. 相似文献
20.
C. Du G. E. Hart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(5):645-653
Triticum turgidum L. var ‘durum’ cv ‘Langdon’-T. t. var ‘dicoccoides’ chromosome 6A and 6B recombinant substitution lines (RSLs) and a F2 population derived from a ‘Langdon’-T. t. var ‘dicoccoides’ disomic chromosome 6A substitution lineבLangdon’ cross were analyzed with the objective of markedly increasing
the number of markers assigned to and the resolution of previously constructed 6A and 6B linkage maps. Fifty-seven markers
were added to the 6A RSL-population map, which now consists of 73 markers that span 111 cM, and 40 markers were added to the
6B RSL-population map, which now consists of 56 markers that span 123 cM. With the exception of 2 6B loci, all of the loci
on the two RSL-population maps were ordered at a LOD score ≥3.0. Thirty-seven orthologous markers were mapped in the two chromosomes
and colinearity between them is strongly indicated. The 6A RSL-population map and the F2-population map are highly similar, indicating that the former population, which consists of 66 lines, can be reliably used
for mapping, as was previously demonstrated for the 6B RSL population. In the absence of selection and genetic drift, the
lines in a RSL population, except at loci in the substituted/recombined chromosome, should be near-isogenic. An unexpected
finding was that at least 26 and possibly 29 of the RFLPs detected in the RSL populations (18% of the markers analyzed) are
not located in the substituted/recombined chromosomes. Linkage analysis of the markers disclosed that at least 19 of them
are located in six or seven segments that span approximately 10 cM and 17 cM of the genetic lengths of 6B and 6A, respectively,
in the 6A and 6B RSL populations, respectively, a finding that suggests that 40 or more alien segments spanning 8–15% of the
genetic length of the 13 unsubstituted chromosomes are present in both of the RSL populations. Alien alleles are fixed in
many RSLs for most of the markers, in most cases at a frequency consistent with theoretical expectations. Highly distorted
segregation favoring the alien allele was detected for all of the markers in 2 of the segments, however. Nine of the markers
were among those mapped in the substituted/recombined chromosomes; the linkage data obtained for the other 10 was sufficient
to assign them to approximate map positions.
Received: 12 June 1997 / Accepted: 6 October 1997 相似文献