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1.
L-Tyrosine carboxy-lyase (E. C. 4. 1. 1. 25) was extracted fromthe roots of barley seedlings and purified approximately 25fold. Optimum pH for the enzyme activity was found to be 7.3.The Km value for L-tyrosine was calulated as 4.5?104M.D-Isomer did not react with the enzyme. L-DOPA, m-tyrosine ando-tyrosine were decarboxylated to some extent. Pyridoxal phosphateactivated the enzyme 4 fold. Caffeic acid and p-coumaric acidare competitive inhibitors. Ki values were 4.5?105M forcaffeic acid and 1.6?104M for p-coumaric acid. L-DOPAand m-tyrosine had an inhibitory effect on the decarboxylationof L-tyrosine. Hydroxylamine, semicarbazide, p-CMB, Fe++, Cu++,and Hg++ inhibited the decarboxylation of tyrosine. Enzyme activitywas also found in extracts from Triticum aestivum, Zea maysand Cytisus scoparius. (Received November 30, 1973; ) 相似文献
2.
Ritchie Raymond J.; Trautman Donelle A.; Larkum A.W.D. 《Plant & cell physiology》1997,38(11):1232-1241
Phosphate uptake rates in Synechococcus R-2 in BG-11 media (anitrate-based medium, not phosphate limited) were measured usingcells grown semi-continuously and in continuous culture. Netuptake of phosphate is proportional to external concentration.Growing cells at pHo 10 have a net uptake rate of about 600pmol m2 s1 phosphate, but the isotopic flux for32P phosphate was about 4 nmol m2 s1. There appearsto be a constitutive over-capacity for phosphate uptake. TheKm and Vmax, of the saturable component were not significantlydifferent at pHo 7.5 and 10, hence the transport system probablyrecognizes both H2PO4and HPO24. The intracellularinorganic phosphate concentration is about 3 to 10 mol m3,but there is an intracellular polyphosphate store of about 400mol m3. Intracellular inorganic phosphate is 25 to 50kJ mol1 from electrochemical equilibrium in both thelight and dark and at pHo 7.5 and 10. Phosphate uptake is veryslow in the dark ( 100 pmol m2 s1) and is light-activated(pHo 7.51.3 nmol m2 s1, pHo 10600 pmol m2s1). Uptake has an irreversible requirement for Mg2+in the medium. Uptake in the light is strongly Na+-dependent.Phosphate uptake was negatively electrogenic (net negative chargetaken up when transporting phosphate) at pHo 7.5, but positivelyelectrogenic at pHo 10. This seems to exclude a sodium motiveforce driven mechanism. An ATP-driven phosphate uptake mechanismneeds to have a stoichiometry of one phosphate taken up perATP (1 PO4 in/ATP) to be thermodynamically possible under allthe conditions tested in the present study. (Received June 16, 1997; Accepted September 4, 1997) 相似文献
3.
In disbudded epicotyl cuttings taken from light grown 5-dayold Azukia angularis Phaseolus angularis) seedlings, all adventitiousrootlets appeared on the second day of incubation. No root primordiawere observed within the first 24 hr and no increase in thenumber of roots occurred after 48 hr. Puromycin (5.5?105M), p-fluorophenylalanine (1?103M),2-thiouracil (2.3?104M) and 2,6-diaminopurine (2?105M)inhibited rooting when applied to cuttings on the second day,but showed no inhibition when applied on the first day. Unlike these inhibitors, pyrithiamine (7.2?105M) inhibitedrooting when it was applied to cuttings on the first day. A rooting promoting effect was observed with actinomycin D (2.4?106M),2,4-dinitrophenol (3?105M) and p-fluorophenylalanine(1?104M) applied to the cuttings on the first day, whereasindoleacetic acid (1.7?104M) showed its promoting effectmost effectively on the second day.
1Contribution No. 17 from the Botanical Gardens, Faculty ofScience, University of Tokyo, Tokyo, Japan. (Received June 4, 1969; ) 相似文献
4.
Effects of indoleacetic, p-chlorophenoxyisobutyric and 2,4,6-trichlorophenoxyacetic acids on three phases of rooting in Azukia cuttings 总被引:1,自引:0,他引:1
In adventitious root formation of disbudded epicotyl cuttingstaken from light-grown, 5-day-old Azukia angularis seedlings,indoleacetic acid (IAA), 1 x 104 M, applied during thefirst day showed no effect, but enhanced the effect of IAA,1 x 104 M, applied during the second day. Treatment duringthe second day promoted rooting by about 70%, and a combinationof treatments for the first and second days promoted rootingsome 200%. p-Chlorophenoxyisobutyric acid (PCIB), 3 x 104M, and2,4,6-trichlorophenoxyacetic acid (2,4,6-T), 2 x 1044M, applied the first day also enhanced the effect of IAA, 2x 104 M, applied the second day. When applied the second day, PCIB, 2 x 104M, increasedthe number of root primordia or clusters of small cells, butnot die number of protruded roots. Formation of the cell clusterwas inhibited by 2,4,6-T, 3 x 104M, applied the secondday. Rooting processes in Azukia cuttings seem to include at leastthree phases: the first phase is induced not only by IAA butalso by PCIB or 2,4,6-T, the second phase is induced by IAAor PCIB and the diird phase depends specifically on IAA. (Received October 28, 1970; ) 相似文献
5.
1. Polyphenol oxidase (o-diphenol : O2 oxidoreductase; E.C.1.10.3.1
[EC]
) was isolated from the other phenolases which werepresent in root-forming carrot callus, and its properties wereexamined. 2. The enzyme was purified about 45-fold over crudeextracts (precipitates between 4070% saturation widiammonium sulfate) by a combination of Bio-gel filtration, protein-bagfiltration, and carboxymethyl cellulose chromatography. Thepurified oxidase was homogeneous according to polyacrylamidegel electrophoresis and Sephadex gel filtration. It was confirmedby CM-cellulose chromatography that the enzyme was absent incallus tissues without accompanying redifferentiation. 3. Themolecular weight of this oxidase was estimated to be 110,000-120,000 from molecular weight-mobility profiles on polyacrylamidegels containing sodium dodecyl sulfate and molecular size-elutionvolume correlations on Sephadex G-150 columns. 4. The enzymeoxidized o-diphenols but showed no detectable activity againstmonophenols. Pyrocatechol, dopamine, caffeic acid, and chlorogenicacid were effectual substrates of the enzyme with Km valuesranging from 103 M to 105M. The enzyme effectivelycatalyzed the oxidation of o-diphenols over the range of pH6.0 to 7.0 and was readily inactivated by heating. The enzymeactivity was slightly influenced by increasing ionic strength.The initial rate of the enzymic reaction was enhanced by additionof Cu2+, Co2+ and Mn2+ ions, and was reduced in the presenceof DTT, PCMPS, glycylglycine, and DIECA. (Received June 17, 1978; ) 相似文献
6.
1. Photophosphorylation was measured with spinach grana sampleswashed by 0.8 M Tris buffer at pH 8.0, which no longer catalyzedthe ferricyanide and NADP HILL reactions with water as the electrondonor. The photophosphorylation with the reaction mixture containing2 104 M 2,6-dichlorophenol indophenol (DCPI) plus above2 103 M ascorbate as the electron donor system insteadof water under anaerobic conditions was, in the most part, dependenton the addition of both PPNR (a nonheme iron protein requisitefor photosynthetic pyridine nucleotide reduction ; spinach ferredoxin)and NADP as the electron acceptor system. However, when ascorbateconcentration only was lowered to 2 104 M, the entirephotophosphorylation proceeded, even in the absence of the electronacceptor system. 2. When the NADP added in the reaction mixture had been reducedby glucose-6-phosphate and glucose-6-phosphate dehydrogenasebefore illumination, the photophosphorylation with 2 104M DCPI plus 6.7 103 M ascorbate decreased to aboutthe same rate as that obtained without NADP. 3. The time course for photophosphorylation in the presenceof NADP was consistent with the time course for the photoreductionof NADP: On the complete reduction of NADP, the photophosphorylationstopped. 4. In the presence of 6.7 103 M dichloropheny 1.1,1-dimethylureaor 3 104 M o-phenanthroline, non-cyclic photophosphorylationwith 2 104 M DCPI plus 6.7 103 M ascorbateas the electron donor system decreased to about half that ofthe control, and the remaining activities were hardly affectedeven at higher concentrations of both inhibitors. The P/2eratios of non-cyclic photophosphorylation in the absence andpresence of ophenanthroline were 0.74 and 0.48, respectively.
1Present address: Department of Biology, University of California,San Diego, La Jolla, California 92037, U. S. A. 相似文献
7.
Astle, M. and Rubery, P. 1987. Carrier-mediated ABA uptake bysuspension-cultured Phaseolus coccineus L. cells: Stereospecificityand inhibition by ionones and ABA esters.J. exp. Bot.38: 150163. The substrate for the abscisic acid (ABA) carrier in Phaseoluscoccineus L. suspension-cultured cells is shown to be the (S)ABAenantiomer, Km = 1?0 mmol m3. The methyl (MeABA) andphenyl (PheABA) esters of ABA inhibit carrier-mediated uptakeof ABA with half-maximal inhibition achieved at about 7?0 mmolm3 and 10 mmol m3 respectively: with (S)MeABAthis value is decreased to about 2?0 mmol m3. There isno demethylation of radioactive MeABA by the cells during 5min incubations. Although MeABA reversibly inhibits the ABAcarrier, it is not a transport substrate: association of radioactiveMeABA with living cells is unaffected by non-radioactive MeABAor ABA and, by comparison with frozen-and-thawed cells, it isshown that the radioactivity remains extracellular. It is proposedthat MeABA binds to the carrier to form an abortive complexthat is not translocated. The terpenoid ABA analogue LAB 144143also inhibits carrier-mediated ABA uptake. At concentrationsup to about 20 mmol m3 - and ß-ionone specificallyinhibit the ABA carrier with the half-maximal effect at about0?6 mmol m3 ß-ionone. However, at higher iononeconcentrations, the uptake of ABA, indol-3-yl acetic acid andof 5,5-dimethyloxazolidine-2,4-dione (DMO) are all stimulated:this may reflect general permeabilization of the membrane toweak acids by ionone. Key words: Uptake carrier, abscisic acid, methyl and phenyl esters of ABA, ionone, Phaseolus coccineus L. suspension culture 相似文献
8.
In azuki bean (Azukia angularis = Vignia angularis) epicotylsections, 5 ? 104 M coumarin inhibited the incorporationof radioactivity from [U14C]glucose into the cellulosefraction by 35% in the absence of indole-3-acetic acid (IAA)and by 40% in the presence of 1 ? 104 M IAA. There wasno inhibitory effect on the incorporation of radioactivity intothe other fractions. Coumarin at 5 ? 104 M reversed thepromoting effect of 1 ? 105 M gibberellin A3 (GA) andthe inhibitory effect of 1 ? 105 M kinetin on IAA-inducedelongation of sections with no significant effects on IAA-inducedelongation. Neither GA nor kinetin had any appreciable effectson cellulose synthesis. No inhibition of cellulose syntheiswas observed with 1 ? 103 M colchichine, which has beenreported to have effects similar to those of coumarin on GA-or kinetin-affected stem elongation. Coumarin at 5 ? 104M was ineffectual in breaking up wall microtubules, while adisrupting effect on wall microtubules was clearly demonstratedwith 3 ? 104M colchicine. From these results, the possible involvement of cellulose synthesisin cell expansion controlled by GA or kinetin was suggested. (Received August 3, 1973; ) 相似文献
9.
Seminal roots of Zea mays L. show curved growth, i.e. waving,meandering and spiral growth, when water cultured. Root curvaturewas accelerated by exogenously applied indole-3-acetic acidat 109 M and gibberellic acid at 106M; this curvaturedisappeared when 107 M p-chlorophenoxyisobutyric acidwas added. Roots curved more when the tops of seedlings wereexposed to light than when the tops of seedlings were covered.These results suggest that auxin may induce root curvature. (Received February 29, 1980; ) 相似文献
10.
L-Leucine Transport in Isolated Protoplasts of Vinca Suspension Culture: Characterization of Uptake 总被引:1,自引:0,他引:1
The uptake of L-leucine into Vinca protoplasts was studied undervarious conditions. The uptake was highly pH-dependent, withthe optimal pH between 3.0 and 4.0. The uptake was also energydependent, since azide, 2,4-dinitrophenol (DNP), carbonyl cyanidem-chlorophenyl hydrazone (CCCP), and iodoacetate inhibited theuptake. Oligomycin, N,N'-dicycIohexyI carbodiimide (DCCD) andvanadate, but not ouabain, inhibited the uptake, suggestingthat ATPase for H+ electrogenic extrusion was necessary to theuptake of L-leucine. The uptake showed stereospecificity, butwas partially inhibited by other L-amino acids. A kinetic studyof the uptake showed that the uptake was multiphasic with threesaturable phases and one unsaturable phase which occurred atconcentrations of L-leucine over 1 mM. The Km values of thethree affinity sites were 1.4 x 103 M, 1.3 x 104M, 4.3 x 105 M; the maximum velocity values were 3.3x 108, 4.5 x 109, 1.8 x 109 mol/10 min/4x 106 cells. (Received April 18, 1981; Accepted August 25, 1981) 相似文献
11.
Effects of respiratory inhibitors on frond and flower productionin light culture of a long-day duckweed, Lemna gibba G 3, wereinvestigated. The inhibitors examined could be divided into3 groups based on their specific actions: (A) 2,4-Dinitrophenol(106M), arsenate (104M), malonate (102M),o-phenanthroline (106M), ,'-dipyridyl (105M) andazide (106M) inhibited flower production by suppressingthe rate of flower production without affecting the inductionperiod. Frond production, however, was promoted by these reagents.Effective time of application came one day after the end ofthe induction period. (B) Iodoacetate (106M) and fluoride(104M) inhibited both flower production and, less significantly,frond production. Reduced rate of flower production was responsiblefor the inhibition of flowering. Effective time of applicationpreceded by one day that of A group inhibitors. (C) Salicylaldoxime(106M), diethyldithiocarbamate (106M) and 8-hydroxyquinoline(107M) enhanced flower production by reducing the lengthof the induction period, and simultaneously slightly inhibitedfrond production. Effective time of application was the latterhalf of the induction period. The implications of these findingsare discussed with special reference to the component processesinvolved in photoperiodic induction of flowering in duckweed. (Received March 27, 1969; ) 相似文献
12.
- Heliangine at 104M promoted the adventitious root formationin hypocotyls of cuttings taken from light-grown (1,900 lux)seedlings of Phaseolus mungo. The promotion was almost completelyreduced by simultaneously supplied 3104M cysteine or1.5104M cystine, but not suppressed by 3104Mof reduced glutathione, alanine or serine.
- A 4 hr pretreatmentwith 3104M cysteine made Phaseoluscuttings less sensitiveto heliangine, but cysteine suppliedafter the treatment withheliangine brought about no effecton the action of heliangine.
- Cysteine also removed the inhibiting effect of heliangineonthe indoleacetic acid-induced elongation of etiolated Avenacoleoptile sections.
- In an aqueous solution heliangine formedan addition productwith cysteine, indicating that cysteinecan inactivate helianginewithout any biological processes.
- On Phaseolus adventitious rooting, no effect was observedofp-chloromercuribenzoic acid, N-ethylmaleimide, 1,4-naphthoquinone,coumarin or penicillin. Reactivity toward sulfhydryl groupsalone does not qualify a substance to be a promotor of rootformation.
- Maleic hydrazide at 104M promoted root formation,butits effect was not removed by cysteine.
13.
The effects of abscisic acid (ABA) treatments on the surfaceelectrical properties of cells and isolated protoplasts fromCatharanthus roseus cell suspension cultures were studied byelectrophoretic mobility and 9-aminoacridine (9AA) fluorescencemeasurements. The surface charge densities of the cells andprotoplasts estimated from electrokinetic data were 0.064Cm2and 0.048 C m2 respectively. These values wereclose to that estimated by 9AA fluorescence technique i.e.,0.053 Cm2 for the cells and 0.041 Cm2for the isolated protoplasts accordingly. The net negative surfacecharge density decreased after application of 10 µM and50 µM ABA in both cells and protoplats, the more pronouncedeffect being observed at 10 µM ABA. When 100 µMABA was supplemented to the cell suspension culture the oppositeeffect was observed. The average charge density increased to0.074 C m2 for the cells, and to 0.055C m2 for protoplasts, as revealed from the 9AA measurements.The results are discussed in terms of specific concentrationdependent ABA-induced alterations of the electrostatic propertiesof cell and protoplast membranes. (Received December 12, 1994; Accepted April 3, 1995) 相似文献
14.
The prediction that very high seed yields of dry beans (Phaseolusvulgaris L.) would be produced by the delayed transplantingof large plants has been tested in a factorial experiment withfour dates of transplanting and eight plant populations. Therewere significant differences in yield between transplantingdates and between population densities, and there was a significantdate-density interaction. At low plant densities (up to about30 plants m2) the three transplanted treatments yieldedless than the hand-sown controls, and late transplanting yieldedless than early. At the highest density the situation was reversed;all three transplanted treatments out-yielded the controls andlate transplanting tended to out-yield plants transplanted early.The biggest yield was 340 g seed m2 from a transplantedcrop grown at 35 plants m2. The data on yield fitted a modified rectangular hyperbola ofthe form where y is yield per unit area, p is the number of plants perunit area, t is the number of days between sowing and transplanting,and Bo, n, m, and p are arbitrary parameters. This equationaccounted for 91 per cent of the variation in yield with t andp. It is suggested that late transplanting had adverse effects,due to transplanting shock and which were mostmarked at low plant densities; and beneficial effects, ascribableto an effect on plant plasticity, which were mostmarked at high plant densities. Possible physiological mechanismsof these effects are discussed. Phaseolus vulgaris, yield, density, transplanting 相似文献
15.
Five inbred strains (129/J, BALB/cByJ, C3HeB/FeJ, C57BI/6J andDBA/2J) were examined with two-bottle (48 h) preference ratiotesting across concentrations of sodium saccharin (3 x 104M, 103 M, 3 x 103 M and 102 M), d-phenylalanine(103 M, 102 M and 101 M), and l-glutamine(102 M, 3 x 102 M, 101 M and 3 x 101M). Three consistent groupings of strains were observed acrosssubstances and concentrations:
- C57BI/6J (preference at low andhigh concentrations);
- BALB/cByJ and C3HeB/FeJ (preferenceat high concentrations);
- 129/J and DBA/2J (preference at highconcentration for sodiumsaccharin and indifference to d-phenylalanineand l-glutamine).
16.
Efficiency of Potassium Utilization by Barley Varieties: The Role of Subcellular Compartmentation 总被引:6,自引:3,他引:3
RAZAQUE MEMON ABDUL; SACCOMANI MASSIMO; GLASS ANTHONY D. M. 《Journal of experimental botany》1985,36(12):1860-1876
Memon, A. R., Saccomani, M. and Glass, A. D. M. 1985. Efficiencyof potassium utilization by barley varieties: The role of subcellularcompartmentation.?J. exp. Bot. 36: 18601876. The subcellulardistributions of K+ in roots of three barley (Hordeum vulgareL.) varieties, grown at 10 and 100 mmol m3 external K+([K+]o) were estimated by compartmental analyses. In general,increased [K+]o caused a 23 fold increase in vacuolar[K+], but cytoplasmic [K+] increased only slightly. Nevertheless,the three varieties, which had been selected for study on thebasis of their different rates of K+ utilization, showed distinctdifferences in the allocation of K+ between cytoplasm and vacuole.At 10 mmol m3 [K+]o var. Betzes exhibited typical K+deficiency symptoms while var. Fergus and var. Compana did not,even though Betzes had higher [K+] in shoots and roots. Theinefficient utilization of K+ in this variety appears to beassociated with a failure to mobilize vacuolar K+ into the cytoplasmiccompartment (the ratio of vacuolar: cytoplasmic K+ contentsfor Betzes was 4.1 compared to 2.7 and 2.5, respectively, forFergus and Compana). Fergus and Betzes, which demonstrate pronouncedgrowth responses to increased [K+]0 between 10 and 100 mmolm3, showed significant increases of cytoplasmic [K+]in this range of [K+]o. By contrast, cytoplasmic [K+] in Compana,a variety whose growth is not stimulated by increased [K+]0(from 10 to 100 mmol m3) showed virtually no increase.It is suggested that the efficiency of K+ utilization and thegrowth response to [K+]0 in these varieties are functions ofthe subcellular distribution of this ion between cytoplasm andvacuole. Key words: Barley varieties, K+ subcellular compartmentation, utilization efficiency 相似文献
17.
Bull R Marengo JJ Finkelstein JP Behrens MI Alvarez O 《American journal of physiology. Cell physiology》2003,285(1):C119-C128
We have reported that ryanodine receptor (RyR) channels display three different responses to cytoplasmic free Ca2+ concentration ([Ca2+]) depending on their redox state (Marengo JJ, Hidalgo C, and Bull R. Biophys J 74: 12631277, 1998), with low, moderate, and high maximal fractional open times (Po). Activation by ATP of single RyR channels from rat brain cortex was tested in planar lipid bilayers with 10 or 0.1 µM cytoplasmic [Ca2+]. At 10 µM [Ca2+], low-Po channels presented lower apparent affinity to activation by ATP [[ATP] for half-maximal activation (KaATP) = 422 µM] than moderate-Po channels (KaATP = 82 µM). Oxidation of low-Po channels with thimerosal or 2,2'-dithiodipyridine (DTDP) gave rise to moderate-Po channels and decreased KaATP from 422 to 82 µM. At 0.1 µM cytoplasmic [Ca2+], ATP induced an almost negligible activation of low-Po channels. After oxidation to high-Po behavior, activation by ATP was markedly increased. Noise analysis of single-channel fluctuations of low-Po channels at 10 µM [Ca2+] plus ATP revealed the presence of subconductance states, suggesting a conduction mechanism that involves four independent subchannels. On oxidation the subchannels opened and closed in a concerted mode. subconductance states; calcium ion release channels; calcium ion regulation; thimerosal; 2,2'-dithiodipyridine 相似文献
18.
Guttation was used as a non-destructive way to study the flowof water and mineral ions from the roots and compared with parallelmeasurements of root exudation. Guttation of the leaves of barley seedlings depends on age andon the culture solution. Best rates of guttation were obtainedwith the primary leaves of 6- to 7-day-old seedlings grown onfull mineral nutrient solution. The growing leaf tissue becomessaturated with K+ below 1.5 mM K+ in the medium, whereas K+concentration in the guttated fluid still increases furtheras K+ concentration in the medium is raised. At 3 mM K+ averagevalues of guttation were 1.42.4 mm3 h1 per plantwith a K+ concentration of 1020 mM; for exuding plantsthe flow was 4.27.6 mm3 h1 per plant and K+ concentration3555 mM. Abscisic acid (ABA) at 106 to 104 M 02h after addition to the root medium increased volume flow ofguttation and exudation and the amount of K+ exported. Threeh after addition of ABA both volume and amount of K+ were reduced.There was an ABA-dependent increase in water permeability (Lp)of exuding roots shortly after ABA addition. Later Lp was decreasedby 35 per cent and salt export by 60 per cent suggesting aneffect of ABA on salt transport to the xylem apart from itseffect on Lp. Benzyladenine (5 x 108 to 105 M)and kinetin (5 x 106 M) progressively reduced volumeflow and K+ export in guttation and exudation and reduced Lp. Guttation showed a qualitatively similar response to phytohormonesas found here and elsewhere using exuding roots. Hordeum vulgare L., barley, guttation, abscisic acid, cytokinins, benzyl adenine, kinetin 相似文献
19.
Internodal cells of Nitellopsis were made tonoplast-free byperfusion with a medium containing EGTA. Cytoplasmic concentrationsof solutes were controlled by a second perfusion with mediaof known composition. The electrogenic pump current (Ip), whichwas calculated from electrical data obtained from cells withand without ATP, was compared with the current carried by H+(IH+) across the plasma membrane. A close correlation betweenIp and IH+ was found under various internal and external conditions.(1) Ip and IH+ depended on the internal ATP and showed Michaelis-Mententype saturation curves. For Ip, Km was 120 µM and themaximum current Vmax was 15.1 mA m2, while for IH+, Kmwas 160 µM and Vmax was 16.6 mA m2. (2) Ip andIH+ showed almost the same IH2+ dependence. The Mg2+-dependentIp was 19.5 mA m2, while the Mg2+-dependent IH2+ was17.7 mA m2. (3) IH2+ was maximal at an external pH of8 and decreased both in acidic and alkaline pH ranges. Ip wasnearly equal to IH+ in the pH range between 8 and 5. (4) IH+became maximal at an internal pH of 7.3, which is nearly thesame as the pH for maximal electrogenecity found by Mimura andTazawa (1984). All these facts support the idea proposed in our previous paper(Takeshige et al. 1985) that the electrogenic ion pump locatedin the plasma membrane of Nitellopsis is the H+ pump.
1 Dedicated to Professor Dr. Erwin Bünning on the occasionof his 80th birthday. (Received June 21, 1985; Accepted December 20, 1985) 相似文献
20.
Protoplasts were successfully isolated from internodal callustissues of both Oxalis glaucifolia and O. rhombeo-ovata whenthey were digested in a solution containing 0.1% (w/v) MacerozymeR-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m3sucrose. Protoplasts proliferated to give cell colonies on Gamborget al.'s B5 medium supplemented with 0.3 mmol m3 mannitol,0.5 mg dm32, 4-D, and 2.0 mg dm3 kinetin. Calluswas produced upon transfer of cell colonies to Murashige andSkoog medium containing 2.0 mg dm3 l-naphthaleneaceticacid (NAA) and 0.1 mg dm3 kinetin for O. glaucifolia,or with 5.0 mg dm3 NAA and 0.5 mg dm3 6-benzylaminopurine,for O. rhombeo-ovata. Plants were regenerated from O. glaucifoliaprotoplasts on a medium containing 0.1 mg dm3 NAA, 1.0mg dm3 kinetin and 1.0 mg dm3 gibberellic acid,but only vascular nodules were differentiated by O. rhombeo-ovataprotoplast-derived calli. Key words: Tissue culture, protoplasts, plant regeneration, Oxalis spp 相似文献