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1.
Monika Łysakowska Andrzej Denys Monika Sienkiewicz 《Central European Journal of Biology》2011,6(3):405-413
The aim of this work was to investigate the antimicrobial properties of thyme essential oil against clinical multiresistant
strains of Acinetobacter spp. The antibacterial activity of oil was tested against standard and clinical bacterial strains of Acinetobacter genus. The agar diffusion method was used to check the inhibition of microbial growth at various concentrations of the oil
from Thymus vulgaris. Susceptibility testing to antibiotics and chemotherapeutics was prepared using the disc-diffusion method. Identification
of bacterial strains was carried out with the Vitek system and confirmed by PCR for Acinetobacter baumanii gyrB gene. The results of experiments showed that the oil from T. vulgaris exhibited an extremely strong activity against all of the clinical strains of Acinetobacter. Thyme oil demonstrated a very good efficacy against multiresistant strains of tested bacteria. Essential oils seems to be
an excellent alternative for synthetic preparations and that is reason for an extensive assessment of their antimicrobial
activity.
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2.
Éva Hideg 《Central European Journal of Biology》2008,3(3):273-284
Four fluorescent singlet oxygen sensors: DanePy, its oxalate salt, Singlet Oxygen Sensor Green and MVP, were infiltrated into
tobacco leaves and tested for toxicity, subcellular localization, light sensitivity and capacity to trap the singlet oxygen
produced in photoinhibition. For reference, a broad sensitivity free radical probe, TEMPO-9-AC, was also included. Photochemical
yield was approximately 15% and 10% inhibited by Singlet Oxygen Sensor Green and MVP, respectively, but was not significantly
affected by the other probes. Under photoinhibitory conditions, brought about by irradiating lincomycin-treated leaves with
strong photosynthetically active radiation, DanePy and Singlet Oxygen Sensor Green were responsive. Singlet Oxygen Sensor
Green was also reactive to low, non-photoinhibitory light exposure of the leaf, which was not characteristic to the other
probes. MVP did not respond to singlet oxygen which can partly be explained by a possible attenuation of its blue emission
in the leaf, as shown by the example TEMPO-9-AC. DanePy-oxalate did not respond to photosynthetic singlet oxygen due to lack
of its penetration into photosynthetic tissue and hence could be useful in detecting any singlet oxygen which escapes from
a chloroplast initiation site. DanePy was localized in the chloroplasts, while Singlet Oxygen Sensor Green was mainly found
in the epidermal cells preferentially associated with the nucleus.
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Ilona Faustova Aleksei Kuznetsov Erkki Juronen Mart Loog Jaak Järv 《Central European Journal of Biology》2010,5(2):135-142
Among four pyruvate kinase isoenzymes, M1, M2, R and L, only M1 is considered as a nonallosteric enzyme. However, here we
show that the non-phosphorylated L-type pyruvate kinase (L-PK) is also a non-allosteric enzyme with respect to its substrate
phosphoenolpyruvate (PEP). The allosteric catalytic properties of L-PK are switched on through phosphorylation by cAMP-dependent
protein kinase. The non-phosphorylated enzyme was produced by expressing the rat L-PK in E. coli, as the bacterium does not have mammalian-type protein kinases. The resulting tetrameric protein was phosphorylated with
a stoichiometric ratio of one mole of phosphate per one L-PK monomer. Activity of the phosphorylated enzyme was allosterically
regulated by PEP with the Hill coefficient n=2.5. It was observed that allostery was engaged by phosphorylation of the first
subunit in the tetrameric enzyme, while further phosphorylation only modulated this effect. The discovered switching between
non-allosteric and allosteric forms of L-PK and the possibility of modulating the allostery by phosphorylation are important
for understanding of the interrelationship between allostery and the regulatory phosphorylation in general, and may have implication
for further analysis of glycolysis regulation in the liver.
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5.
Akiko Takenaka-Uema Norasuthi Bangphoomi Chieko Shioda Kazuyuki Uchida Fumihiro Gen Kentaro Kato Takeshi Haga Shin Murakami Hiroomi Akashi Taisuke Hoimoto 《中国病毒学》2016,31(3):274-277
正Dear Editor,Akabane virus(AKAV),an orthobunyavirus,is transmitted primarily by biting midges and is widely distributed throughout the world except the Europe.AKAV was first isolated from mosquitoes in Japan(Oya et al.,1961).Although pregnant cows,ewes,and goats infected with AKAV exhibit no clinical signs of disease,in utero infections result in abortion,premature birth,stillbirth,and 相似文献
6.
Ahmad Nejati Mohammad Farahmand Hamideh Tabatabaie Maryam Yousefi Yaghoob Mollaei-Kandelous Shohreh Shahmahmoodi 《中国病毒学》2017,32(3):249-252
<正>Dear Editor,Acute flaccid paralysis(AFP)is a complex syndrome often caused by polioviruses.While most countries have eradicated wild polioviruses by vaccination,AFP still remains a health problem in these countries.Most studies have highlighted non-polio enteroviruses(NPEVs)as 相似文献
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Abulimiti Moming Yujiang Zhang Chenchen Chang Huan Yu Meifang Wang Zhihong Hu Fei Deng Surong Sun 《中国病毒学》2017,32(1):97-100
<正>Dear Editor,Severe fever with thrombocytopenia syndrome virus(SFTSV)is a newly identified viral pathogen of the genus Phlebovirus in the family Bunyaviridae(Sun et al.,2012).SFTSV was first identified from patient serum samples in China(Li et al.,2013;Ning et al.,2015).SFTSV can cause a severe hemorrhagic fever-like disease with a reported case fatality rate ranging from 2.5% 相似文献
9.
Flavia Adriana Zanetti Romina Cardona Carlos Rodolfo Federico Silvina Chimeno-Zoth Gabriela Calamante 《中国病毒学》2016,31(3):266-269
正Dear Editor,Infectious bursal disease virus(IBDV)causes infectious bursal disease,a highly contagious immunosuppressive disease that affects young chickens and causes economic losses in the poultry industry worldwide.IBDV replicates mainly in actively dividing B lymphocytes within the bursa of Fabricius(BF),leading to immunosuppression in affected flocks(Mahgoub et al.,2012).Viral protein 2(VP2),the only structural component of the IBDV 相似文献
10.
<正>Dear Editor,The main limitation of the viral pathogenesis study of human gammaherpesviruses Epstein-Barr virus(EBV)and Kaposi sarcoma-associated herpesvirus(KSHV)is the absence of animal model owing to the narrow host tropism of both EBV and KSHV.Murine gammaherpesvirus 68(MHV68)encodes several genes involved in 相似文献
11.
Yongxia Shi Kui Zheng Xiaobo Li Liqiang Li Shufen Li Jinmin Ma Jun Dai Jingkai Ji Shuai Yuan Haorong Lu Jiandong Li Fangfang Sun Xun Xu Jicheng Huang 《中国病毒学》2017,32(3):253-256
<正>Dear Editor,Rift Valley fever(RVF)is an anthropozoonosis caused by Rift Valley fever virus(RVFV).RVFV belongs to the Phlebovirus genus in the family Bunyaviridae,which is circulating among ruminants.Human infection with RVFV is generally asymptomatic,however,minority of patients develop severe RVF diseases like encephalitis or 相似文献
12.
正Dear Editor,Since April 2010,an outbreak of a new disease has elicited symptoms of high fever,loss of appetite,and reduction in egg production in layer ducks in eastern China;this phenomenon has now spread throughout China(Cao et al.,2011;Su et al.,2011).The causative agent of the disease was identified as Tembusu virus(TMUV),which was classified into the genus Flavivirus, 相似文献
13.
<正>Dear Editor,Hepatitis C virus(HCV)is a positive-strand RNA virus that belongs to the genus Hepacivirus within the Flaviviridae family.HCV causes chronic liver diseases,and185 million people are infected(Messina et al.,2015).Currently,there is no approved vaccine to prevent hepatitis C.HCV induces autophagy through elevating reactive oxygen species(ROS)levels via the unfolded 相似文献
14.
E. L. Zdorovenko L. D. Varbanets O. S. Brovarskaya O. A. Valueva A. S. Shashkov Yu. A. Knirel’ 《Microbiology》2011,80(3):372-377
The structure and immunochemical properties of lipopolysaccharide from Budvicia aquatica 97U124, a representative of a novel species of Enterobacteriaceae, were studied. The O-polysaccharide (OPS) was isolated by mild acid hydrolysis and characterized by monosaccharide analysis
and NMR spectroscopy. It was shown that the OPS from B. aquatica 97U124 consisted of repeating units with the structure
相似文献
15.
<正>Dear Editor,Hepatitis C virus(HCV)is a major cause of chronic liver diseases and hepatocellular carcinoma(HCC)with about71 million people globally infected.HCV encodes only10 viral proteins and its replication relies on host proteins.Many host factors including ADP-ribosylation factors 相似文献
16.
Peter R. Bahn Aristotel Pappelis John Bozzola 《Origins of life and evolution of the biosphere》2006,36(5-6):617-619
One of the most prominent amino acids to appear in monomer-generating origin-of-life experiments is aspartic acid. Hugo Schiff
found in 1897 that aspartic acid polymerizes when heated to form polyaspartylimide which hydrolyzes in basic aqueous solution
to form thermal polyaspartic acid which is a branched polypeptide. We recently reported at the ISSOL 2005 Conference that
commercially made thermal polyaspartic acid forms microspheres when heated in boiling water and allowed to cool. In a new
experiment we heated aspartic acid at 180°C for up to 100 h to form thermal polyaspartylimide which when heated in boiling
water without addition of base hydrolyzed to form thermal polyaspartic acid which upon cooling formed microspheres. Thermal
polyaspartic acid microspheres appear protocell-like in the sense of being prebiotically plausible lattices or containers
that could eventually have been filled with just the right additions of primordial proteins, nucleic acids, lipids, and metabolites
so as to constitute protocells capable of undergoing further chemical and biological evolution. Thermal polyaspartic acid
microspheres are extremely simple models of protocells that are more amenable to precise quantitative experimental investigation
than the proteinoid microspheres of Sidney W. Fox. We present here scanning electron microscope images of such thermal polyaspartic
acid microspheres. Figure 1 shows thermal polyaspartic acid microspheres from l-aspartic acid heated at 180°C for 50 h, at a magnification of 3,500×. Figure 2 shows thermal polyaspartic acid microspheres from the same sample at a magnification of 7,000×. The thermal polyaspartic
acid microspheres have a diameter of approximately 1 μm These images were viewed with a Hitachi S2460N scanning electron microscope
at 20 kV acceleration voltage.
Figure 1 Thermal polyaspartic acid microspheres from l-aspartic acid heated at 180°C for 50 h, at a magnification of 3,500×.
Figure 2 Thermal polyaspartic acid microspheres from l-aspartic acid heated at 180°C for 50 h, at a magnification of 7,000×.
Presented at: National Workshop on Astrobiology: Search for Life in the Solar System, Capri, Italy, 26 to 28 October, 2005. 相似文献
Figure 2 Thermal polyaspartic acid microspheres from l-aspartic acid heated at 180°C for 50 h, at a magnification of 7,000×.
Presented at: National Workshop on Astrobiology: Search for Life in the Solar System, Capri, Italy, 26 to 28 October, 2005. 相似文献
17.
The multifunctional trans-activator Tat is an essential regulatory protein for HIV-1 replication and is characterized by high sequence diversity. Numerous experimental studies have examined Tat in HIV-1 subtype B, but research on subtype C Tat is lacking, despite the high prevalence of infections caused by subtype C worldwide. We hypothesized that amino acid differences contribute to functional differences among Tat proteins. In the present study, we found that subtype B NL4-3Tat and subtype C isolate HIV1084 i Tat exhibited differences in stability by overexpressing the fusion protein Tat-Flag. In addition, 1084 i Tat can activate LTR and NF-κB more efficiently than NL4-3 Tat. In analyses of the activities of the truncated forms of Tat, we found that the carboxylterminal region of Tat regulates its stability and transactivity. According to our results, we speculated that the differences in stability between B-Tat and C-Tat result in differences in transactivation ability. 相似文献
18.
<正>Dear Editor,Anelloviruses,which are all single-strand circular DNA viruses,have extremely heterogeneous genetic features.To date,the family Anelloviridae is divided into 11genus,and the most dominated species include torque teno virus(TTV),torque teno mini virus(TTMV)and 相似文献
19.
Consumption of 1-hydroxy-2-naphthoic acid by strain Arthrobacter sp. K3 was investigated. Drastic increase in the substrate concentration in flow culture was shown to induce the lag phase of growth in case the initial substrate concentration in the medium was not saturating; the culture originally saturated with the substrate (S K S ) was resistant to the concentration increase. In accordance with the constructed kinetic model, lag phase results from an accumulation of intermediates in the metabolic system.
相似文献
20.
S. F. Arkhipova I. V. Artem’yev E. A. Goryacheva V. Z. Pletnev 《Russian Journal of Bioorganic Chemistry》2008,34(5):544-549
Conformational analysis of two pairs of synthetic cyclodipeptides formed by interaction of both side chain functional groups ( , and ) and of the main and side chains ( , and ) was achieved by the method of molecular mechanics. The energetically optimal conformational states of the molecules under study were determined. It was shown that the conformational motility of cyclic system of the compounds under study depends on the relative arrangement of the amide groups and the number of atoms in the cycle. 相似文献