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1.
Chil-Yong Kang John A. Shadduck 《In vitro cellular & developmental biology. Plant》1977,13(12):849-856
Summary Experimental tumors developed in white Pekin ducks after intramuscular implantation of 20-methylcholanthrene. Cells derived
from the primary tumor were adapted successfully to grow in vitro and have growth characteristics similar to that of established
cell lines of mammalian origin. The cell density rises rapidly and the doubling time is approximately 19 hr. The duck cells
have been cultured succesfully for at least 80 passages in vitro. The continuously cultured cells have the characteristic
chromosome pattern of duck, and the DNA of the duck cell line hybridized with duck liver DNA. We believe we have established
a continuous cell line of avian origin. Electron-microscopic examinations of the tumor cells and RNA-directed DNA polymerase
of the cell-free supernate show no evidence of endogenous virus production.
This study was supported by Public Health Service Research Grants CA 16479 and CA 20012 from the National Cancer Institute
and RR 00890 from the National Institutes of Health. 相似文献
2.
Jill M. Siegfried Karen G. Nelson Jane L. Martin David G. Kaufman 《In vitro cellular & developmental biology. Plant》1984,20(1):25-32
Summary Histochemical techniques have been applied to the identification of cell types cultured from human endometrium. Previous work
from this laboratory characterized two principtal cell types found in cultures of endometrium: a mature epithelial cell and
another cell which was classified as the endometrial stromal cell based on light and electron microscopy. In this report we
compare the histochemical staining of endometrial tissue in frozen sections to that of cultured cells. These results confirm
the epithelial and stromal nature of the respective cell types. Several markers were found that could distinguish between
cells of epithelial and stromal origin. The enzymes alkaline phosphatase, γ-glutamyltranspeptidase, peroxidase, and β-glucuronidase
were localized in glandular and surface epithelia in frozen sections and in colonies of epithelial cells in culture. Stroma
in frozen sections and cultured stromal cells contained leucine aminopeptidase and fibronectin. Epithelial sections and in
culture could also be distinguished from cells of stromal origin by preferential binding of lotus and peanut lectin. Several
other markers were found in both endometrial epithelium and stroma.
J. M. S. was recipient of National Research Service Award CA09156 (National Cancer Institute); K. G. N. was recipient of National
Research Service Award ES07017 (National Institute of Environmental Health Sciences); and D. G. K. was recipient of Research
Career Development Award CA00431 from the National Cancer Institute, Bethesda, MD. Supported by Grant CA 31733 from the National
Cancer Institute, Bethesda, MD. 相似文献
3.
B. Hugh Dorman V. A. Varma Jill M. Siegfried Susan A. Melin Thomas A. Admaec Carol R. Norton David G. Kaufman 《In vitro cellular & developmental biology. Plant》1982,18(11):919-928
Summary Propagable cell cultures derived from human endometrial tissue were determined to contain cells predominantly of stromal cell
origin based on their morphologic resemblance to endometrial stromal cells. These features included nexi, solitary cilia,
and predecidual cytology. In addition to morphology the cell cultures retained a normal karyotype and responded to steroid
hormones as evidenced by cellular aggregation. The stromal cells were evaluated for a variety of characteristics associated
with transformed cells and seemed to be biologically normal without neoplastic phenotypes. Growth potential of the stromal
cell cultures was also characterized in normal maintenance medium, in nutritionally depleted medium with reduced levels of
calcium or serum, and in medium with increased levels of serum. The prolonged survival of the stromal cells in vitro coupled
with the retention of in vivo characteristics and an absence of neoplastic phenotype provides a human cell system that is
amenable to a variety of long-term experimental analyses.
This work was supported by contract CP75956 and grant CA31733 from the National Cancer Institute. B. Hugh Dorman was the recipient
of a predoctoral scholarship from the Chemical Industry Institute of Toxicology. Jill M. Siegfried was supported by National
Research Service Award CA09156. David G. Kaufman is the recipient of a Research Career Development Award (CA00431) from the
National Cancer Institute. 相似文献
4.
In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: Fibroblast-free growth and serial passage 总被引:3,自引:0,他引:3
Michael T. White A. S. L. Hu Susan T. Hamamoto S. Nandi 《In vitro cellular & developmental biology. Plant》1978,14(3):271-281
Summary Normal and neoplastic mouse mammary epithelial cells were cultured in nutrient medium containing D-valine substituted for
L-valine. Fibroblast overgrowth was prevented and epithelial cell functions and morphology were retained in cultures maintained
in, D-valine medium up to 2 months. A nonenzymatic technique was devised to dissociate epithelial cell monolayers. The combined
use of this dissociation buffer and D-valine nutrient medium made it possible to passage serially normal and neoplastic mammary
epithelial cells. Normal cells were derived from mammary glands of animals stimulated with exogenous hormones for various
periods. The period of in vivo hormonal stimulation influenced the ability of normal mammary epithelial cells to attach and
proliferate in primary and serially passaged cultures. A greater proportion of cells derived from glands following 2 to 4
weeks of hormonal stimulation were recovered after replating and showed higher labeling indices during serial passage than
cells from unstimulated or 5- to 7-week stimulated groups.
This investigation was supported by Grant No. CA 05388 from the National Cancer Institute and by Cancer Research Funds of
the University of California. 相似文献
5.
Robert J. Klebe Melodee G. Mancuso 《In vitro cellular & developmental biology. Plant》1983,19(3):167-170
Summary Thirty-one compounds have been identified that act as cryoprotective agents for cultured mammalian cells. Eight compounds
were comparable to dimethylsulfoxide (DMSO) in cryprotective effectivenes. Many of the cryoprotective compounds studied also
(a) promote cell fusion and (b) induce cell differentiation in erythroleukemia and other cell systems. Thus previously unrecognized
effects on the differentiated state of cells may occur when cells are treated with cryoprotective agents.
This study was supported, in part, by grants CA 33074 from the National Cancer Institute, Bethesda, MD, and GM 31056 from
the National Institutes of Health, Bethesda, MD. 相似文献
6.
V. A. Varma Susan A. Melin Thomas A. Adamec B. Hugh Dorman Jill M. Siegfried Leslie A. Walton Charles N. Carney Carol R. Norton David G. Kaufman 《In vitro cellular & developmental biology. Plant》1982,18(11):911-918
Summary Monolayer cultures can be established from human endometrial tissue after enzymatic dispersal into isolated glands or single
cells. Three cell types that have distinct morphology by light and electron microscopy are observed in the resulting primary
cultures. One cell type, an elongated spindle cell, is similar in appearance to fibroblasts derived from other tissues. A
second cell type forms colonies of tightly cohesive cells, ranging in shape from oval to polygonal. These cells have typical
organelles and junctional complexes characteristic of epithelial cells from the endometrium. The third cell type assumes a
pavement-like appearance composed of polygonal cells when viewed by phase contrast microscopy, but lacks distinctive ultrastructural
features of epithelial cells. These cells in culture resemble the endometrial stromal cell, the predominant cell type of the
human endometrium in vivo. The epithelial cell does not survive subculturing but the other two cell types can be passaged
through several generations and can be stored in liquid nitrogen and subsequently returned to culture.
This work was supported by contract N01-CP75956 and grant R01-CA31733 from the National Cancer Institute. V. A. Varma is a
recipient of an American Cancer Society fellowship; B. H. Dorman, a predoctoral fellowship from the Chemical Industry Institute
of Toxicology; J. M. Siegfried, a training grant (CA09156) from the National Cancer Institute; and D. G. Kaufman, a Research
Career Development Award (K04-CA-00431) from the National Cancer Institute. 相似文献
7.
Steve Hamner Walis Jones Jean R. Starkey Howard L. Hosick 《In vitro cellular & developmental biology. Plant》1989,25(12):1107-1113
Summary Three related mouse mammary cell lines were cultured in collagen gels and assayed for growth factor responsiveness and interaction
via soluble factors. The CL-S1 cell line is nontumorigenic and grows poorly in collagen gel culture. The +SA and −SA cell
lines exhibit different degrees of malignant behavior in vivo and have different growth properties in vitro. In collagen gel
culture, +SA growth was stimulated by serum but not by epidermal growth factor (EGF), whereas both serum and EGF were required
for optimal growth of −SA cells of early passage number as well as CL-S1 cells. −SA cells of later passage repeatedly exhibited
a change so as to no longer require serum while retaining EGF responsiveness. [125I]EGF binding analyses indicated that CL-S1 cells bound EGF with less affinity than did −SA cells whereas +SA cells bound
almost to ligand. When cell lines were maintained in separate collagen gels but shared the same culture medium, growth of
+SA or −SA cells was slightly enhanced in the presence of CL-S1 cells and −SA cell growth was enhanced by the presence of
+SA cells. Using the normal rat kidney fibroblast line NRK (clone 49F) as an indicator, serum-containing conditioned media
from each cell line and from each pair of cell lines cultured in collagen gels were tested for transforming growth factor
(TGF) activity. Both the −SA and CL-S1 lines tested positive for TGF-α production and possibly released a TGF-β activity.
These results suggest mechanisms by which cell populations in and around tumors can modify one another’s growth characteristics.
The work was supported by a grant from the American Institute for Cancer Research, by American Cancer Society Institutional
grant IN-119, by funds from the Poncin Trust (Seattle-First National Bank), and by grants CA-39611 and CA46885 from the National
Institutes of Health, Bethesda, MD. 相似文献
8.
In vitro cultivation of human renal cell cancer 总被引:3,自引:0,他引:3
R. D. Williams A. Y. Elliott N. Stein E. E. Fraley 《In vitro cellular & developmental biology. Plant》1976,12(9):623-627
Summary A technique for initiating and propagating epithelial cell cultures of human renal cell cancer and adjacent nontumor kidney
is described. Seventy-five percent of the tumors and 79% of the adjacent kidney specimens cultured with this method have shown
initial outgrowth and have been subcultured at least once. Two renal cell cancer cultures initiated by this method have now
been in continuous culture more than 6 months, have been subcultured 27 and 19 times, and now appear to be stable lines.
The ability to establish long-term in vitro cultures of human renal cell cancers will facilitate studies concerning the immunoreactivity,
cholesterol metabolism, the isolation of renal-cell-cancer-specific antigens, and in vitro chemotherapy testing and will further
our understanding of the basic biology of human renal cell cancer.
American Cancer Society Clinical Fellow 1975–1976.
Supported in part by Grants CA 13095-03 and CA 15551-03 from the National Cancer Institute. 相似文献
9.
Lynne P. Rutzky Beppino C. Giovanella Baldwin H. Tom Celia I. Kaye Philip D. Noguchi Barry D. Kahan 《In vitro cellular & developmental biology. Plant》1983,19(2):99-107
Summary An epithelial cell line, LS123, was established in 1974 from the second in a series of three primary colonic tumors resected
from a Caucasian female. The cell line is aneuploid, releases low concentrations of carcinoembryonic antigen (CEA), fails
to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells. LS123
cells grow on confluent cell monolayers and in either low serum or serum-free medium. In the chick embryonic skin assay, LS123
cells grew as a well-differentiated abnormal colonic epithelium with little mitotic activity but with some indication of invasion.
On floating collagen gels LS123 cells formed a one to three-cell-layer-thick undifferentiated epithelial sheet. The apparent
low invasiveness of the cells of this line is supported by the patient's history of three primary colon tumors without systemic
metastases during the past 30 yr. Therefore, although LS123 cells possess several properties associated with neoplasia, they
have little invasive potential. Thus, LS123 cells may represent an important model for the study of human colon cancer.
Presented in part at the 33rd Annual Meeting of the Tissue Culture Association, San Diego, CA, June 6–10, 1982.
The work has been partially supported by U.S. Public Health Service Grants CA23871 (L. P. R.), CA24024 and RCDAK04-CA00579
(B. H. T.), and CA27124 and CA22370 (B. D. K.); the latter was awarded through the National Large Bowel Cancer Project. 相似文献
10.
K. A. Rafferty Jr. R. L. Ruben S. K. Young 《In vitro cellular & developmental biology. Plant》1978,14(2):227-235
Summary Normal cultures of epithelial appearance were initiated by trypsinization of a surgically resected, histologically normal
branchial cyst. Cellular morphology was consistent with derivation from the stratified squamous epithelium of the cyst or
from vascular endothelium, although electron micrographs of the cultured cells failed to show any junctional complexes. Infection
with SV40 produced transformants which were also epithelioid in appearance. These grew vigorously for 22 to 50 population
doublings (about 23 to 32 subcultures, depending upon regimen) and then became quiescent. During this evolution, virus was
detectable at all stages by both direct isolation (cell extracts) and cocultivation with permissive cells. In two sublines
in which selection for rapidly growing cell types occurred, virus was detected only by cocultivation. The work confirms that
of others in the finding that normal human epithelial cells are susceptible to transformation by oncogenic viruses, but are
apparently less responsive than are fibroblasts to such transforming agents. It also suggests that subcultivation techniques
that maintain the populations of transformed cells at low density tend to select against cell strains that are continuous
producers of infectious virus.
This study was supported by Grant CA 13494 from the National Cancer Institute. 相似文献
11.
R. L. Ceriani J. Taylor-Papadimitriou J. A. Peterson P. Brown 《In vitro cellular & developmental biology. Plant》1979,15(5):356-362
Summary Two major types of cells can be cultured from early lactation human milks: a colony-forming epithelial cell and an adherent
nondividing cell referred to as a foam cell The epithelial cells show a positive reaction with a specific antiserum reactive
against membrane components of the milk fat globule, whereas the foam cells do not. The nondividing foam cells are phagocytic
and can be killed by silica particles; they produce lysozyme, are resistant to trypsinization, and have Fc receptors. These
properties, together with the lack of reaction with antiserum to the milk fat globule membrane, suggest that the foam cells
are not terminally differential epithelial cells, but tissue macrophages.
R. L. C. was supported by Grant No. Ca 19455 from the National Cancer Institute, a Yamagiawa-Yoshida Memorial International
Cancer Study Grant, and the Imperial Cancer Research Fund. J. A. P. was supported by Grant No. CA 19455 from the National
Cancer Institute. 相似文献
12.
Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix 总被引:6,自引:0,他引:6
Ralph S. Freedman James M. Bowen Albert Leibovitz Sen Pathak Michael J. Siciliano Harry S. Gallager Beppino C. Giovanella 《In vitro cellular & developmental biology. Plant》1982,18(8):719-726
Summary An established cell line, SW756, derived from a primary squamous carcinoma of the uterine cervix is described by its morphology,
ultrastructure, karyotype, genetic signature analysis, HLA typing, and tumorigenesis in the nude mouse. Cultured cells obtained
from the SW756 derived nude mouse tumor also were studied for chromosome and isozyme markers. The original tumor was poorly
differentiated carcinoma with minimal keratinization and is compared with that occurring in the nude mouse after the cultured
cells were inoculated. The nude mouse tumor showed similar histological features, but better differentiation than the original
tumor. Karyotype analysis of SW756 demonstrated a hyperdiploid stem line number and several marker chromosomes (MI-M6). No
HeLa marker chromosomes were identified. The isozyme pattern for SW756 reported by others has been confirmed. The unique chromosome
and isozyme features have been identified repeatedly in the cultured cells and, most importantly, in the post nude mouse culture.
We recommend SW756 as a defined human tumorigenic cell line derived from a primary squamous carcinoma of the uterine cervix.
This investigation was supported in part by Public Health Research Grant CA-06294 from the National Cancer Institute, Department
of Health and Human Services. 相似文献
13.
E. Sidebottom 《In vitro cellular & developmental biology. Plant》1980,16(1):77-86
Summary The lecture reviews some aspects of the work on the analysis of malignancy that have been, and are now being, pursued in the
Dunn School. A brief outline of the early experiments that first demonstrated that the malignancy of mouse tumor cells can
be suppressed by the fusion with normal cells is given, and then two areas of current interest in the laboratory are described.
The first is an attempt to analyze the clinically important property of tumors to metastasize and the second is the work on
the isolation and identification of an abnormal membrane glycoprotein present in tumor cells. In addition the value of cell
fusion methods as a general test of hypotheses of malignancy is emphasized.
Presented in the symposium on Gene Transfer, Differentiation and Neoplasia in Plant and Animal Cells at the 30th Annual Meeting
of the Tissue Culture Association, Seattle, Washington, June 10–14, 1979. This symposium was supported in part by Grant CA
26748 from the National Cancer Institute, DHEW, and Grant RD-67 from the American Cancer Society. 相似文献
14.
W. A. Nelson-Rees R. B. Owens P. Arnstein A. J. Kniazeff 《In vitro cellular & developmental biology. Plant》1976,12(10):665-669
Summary A cell line derived from normal fetal canine thymus (Cf2Th) has been in culture since 1967. During cultivation the cells have
changed morphologically from a fibroblast-like to flat, fusiform appearance and karyologically from diploid (2n=78) with 76
telocentric autosomes to hypodiploid with newly formed atelocentric chromosomes. The cells retain canine characteristic enzyme
activity (G6PD and LDH) as well as cell membrane fluorescence and are free of mycoplasma. High passage cells produce tumors
in ATST mice. No endogenous viruses have been detected in these cells. No original publication exists, to date, on the origin
of this line, but seed stocks thereof have been distributed to many laboratories and the cells have served as experimental
substrates in a number of published works in oncology albeit under different designations. The present information is offered
in order to establish the provenance of this valuable cell line and to list characteristics which may serve to monitor for
its purity and to distinguish it from other existing cell lines of dog origin also in common use.
Supported by Contract No. 1-CP-3-3237 within the Virus Cancer Program, National Cancer Institute, National Institutes of Health. 相似文献
15.
Koichi Hirata Tadashi Oku Aaron E. Freeman 《In vitro cellular & developmental biology. Plant》1982,18(9):789-799
Summary Twenty to twenty-two days postcoitum mouse fetal pancreas organ bits were cultured on the dermal surface of irradiated pigskin
as a substrate. The medium used for long term culture consisted of Eagle’s Minimum Essential Medium with the addition of 10%
bovine serum, 0.02 U/ml insulin, 0.025 μg/ml glucagon, 3.63 μg/ml hydrocortisone, 100 μg/ml soybean trypsin inhibitor or 10−8
M atropine. When the medium lacked trypsin inhibitor or atropine but contained the three hormones, the pigskin support began
to be destroyed after 2 to 4 wk in culture. Thereafter, the cultured cells could not grow and survive on the digested pigskin.
When 10−6
M atropine was added to the medium, amylase secretion from cultured cells and destruction of pigskin were inhibited completely
but pancreas cells could not grow or survive. In contrast, 100 μg/ml soybean trypsin inhibitor or 10−8
M atropine permitted cell growth, permitted amylase secretion from the cultured acinar cells, and prevented the destruction
of pigskin. Under these conditions pancreas cells migrated or grew or both from the organ bits onto the surface of the pigskin
dermis and organoid aggregations formed. Hydrocortisone was needed to permit growth for more than 2 wk. Glucagon and insulin
had additive effects. Light and electron microscopic observations indicated the culture of at least five kinds of cells, i.e.,
duct, acinar, centroacinar, endocrine, and mesenchymal. The majority of cultured cells were duct cells and acinar cells. There
were few mesenchymal cells. Mouse pancreas cells were cultured for at least 12 wk by this method.
This investigation was supported by PHS Grant CA 30220 awarded by the National Cancer Institute, DHHS, Grant 1203M awarded
by the Council for Tobacco Research, Inc., and Grant RD-65 (for equipment) awarded by the American Cancer Society. Nude mice
were provided by Dr. Wendall M. Farrow of Life Sciences, Inc., Resource Laboratory N01, CP6-1005 of the National Cancer Institute. 相似文献
16.
R. T. Morgan L. K. Woods G. E. Moore L. McGavran L. A. Quinn T. U. Semple 《In vitro cellular & developmental biology. Plant》1981,17(6):503-510
Summary A continuous cell line, COLO 346, was established from a liver metastasis in a patient with adenocarcinoma of the gallbladder.
COLO 346 grew as an adherent monolayer of pleomorphic epithelioid cells. COLO 346 cells produced esterone, but no estradiol,
progesterone, or cortisol. No adrenocorticotropic hormone, β-subunit of human chorionic gonadotropin, carcinoembryonic antigen,
or α-fetoprotein production by the cells was detected. Cell doubling time was 36 h. Seven allelic isozymes were assayed. COLO
346 had a chromosome mode of 74 at 21 months postestablishment with 6 marker chromosomes present in 100% of the cells analyzed.
COLO 346 has been in continuous culture for over 2 yr and is available to other investigators for their studies.
This paper was presented in part at the 31 st Annual Meeting of the Tissue Culture Association, June 1–5, 1980. The work was
supported by Grants CA15018 and CA29514 from the National Cancer Institute, and by the Mary B. and L. H. Marshall Fund. 相似文献
17.
A. T. Hood D. Currie S. J. Garte 《In vitro cellular & developmental biology. Plant》1987,23(4):274-278
Summary A new cell line designated NAS 2BL has been established from a rat nasal tumor induced by inhalation of the direct-acting
carcinogen methylmethane sulfonate. The cells are epithelial in morphology, have a generation time of 34 h, require 10% fetal
bovine serum for optimal growth, and exhibit keratinization at confluence. The karyotype is aneuploid, with several marker
chromosomes, and the cells are transformed by the criterion of nude mouse turmorigenicity.
This work was supported by grants CA36342, ES03563 and Center grants ES00260 and CA13343, from the National Institutes of
Health; and Special Institutional grant 00009 from the American Cancer Society 相似文献
18.
Quantitation of human mammary epithelial antigens in cells cultured from normal and cancerous breast tissues 总被引:1,自引:0,他引:1
Masao Sasaki J. A. Peterson R. L. Ceriani 《In vitro cellular & developmental biology. Plant》1981,17(2):150-158
Summary A sensitive radioimmunoassay technique was developed to quantitatite the level of human breast celltype specific antigens
on cells from normal breast and from various established cell lines of breast and nonbreast origins. Polyacrylamide gel electrophoresis
revealed four major proteinaceous components (150,000; 75,000; 60,000; and 48,000) in human milk fat globule membranes that
were used to immunize rabbits in order to elicit antimammary epithelial cell antibody. Antisera obtained were rendered specific
by abosorptions and were able to recognize three specific mammary epithelial components of the breast epithelial cell. Human
mammary epithelial (HME) antigen expression was highest (1290 ng/106 cells) in normal breast epithelial cells from primary cultures of normal breasts. Lower levels (range: 955 to 330 ng/106 cells) were found in breast epithelial cells from cell lines established from cancerous breast tissue. Cells of nonbreast
origins as well as fibroblasts from breast gave much lower values (less than 30 ng/106 cells). On treatment, with trypsin, of two breast epithelial cell lines (MDA-MB-157 and MCF-7) 80 to 85% of their HME antigen
expression was lost, suggesting that a majority of these breast antigens reside on the cell surface.
This work was Supported by Grant PTD-99 from the American Cancer Society, Grant CA19455 and CA20286 from the National Cancer
Institute, and Biomedical Research Support Grant RR05467 from the National Institutes of Health. Most cells used in the present
study were produced with support from National Cancer Institute Contract Y01-CP8-0500, Biological Carcinogenesis Branch, Division
of Cancer Cause and Prevention, under the auspices of the Office of Naval Research and the Regents of the University of California. 相似文献
19.
George E. Moore Robert T. Morgan Laurie A. Quinn Linda K. Woods 《In vitro cellular & developmental biology. Plant》1978,14(3):301-306
Summary A transitional cell carcinoma cell line, COLO 232, was derived from a primary urinary bladder tumor in a Caucasian male. In
culture, COLO 232 retained distinct uroepithelial phenotypic traits and produced both carcinoembryonic antigen and adrenocorticotropic
hormone. COLO 232 had a chromosome mode of 58 and retained the X and Y chromosomes. Ten marker chromosomes were identified.
COLO 232 will be of value for biochemical and immunological studies.
Presented in part at the 28th Annual Meeting of the Tissue Culture Association, June 7, 1977.
This work was supported by Grant No. CA 15018 awarded by the National Cancer Institute, DHEW, and the Mary B. and L. H. Marshall
Fund. 相似文献
20.
A cloned rat thymic epithelial cell line established from serum-free selective culture 总被引:6,自引:0,他引:6
Arthur Piltch Paul Naylor Jun Hayashi 《In vitro cellular & developmental biology. Plant》1988,24(4):289-293
Summary A serum-free system has been developed for selective growth and long-term culture of rat thymic epithelial cells. The growth
media is a modification of McKeehan's WAJC 404, plus insulin, cholera toxin, dexamethasone, and epidermal growth factor. Cultures
have been continuously passaged and maintained for over 6 mo., and a cloned cell line, TEA3A1, has been established. These
cells are epithelial, judging by morphology and ultrastructure, and are positive for A2B5 and thymosin α markers for thymic
endocrine cells.
This work was partly supported by grant PCM-834 0582 from the National Science Foundation, Washington, DC, and grant P01 CA
37589-2 from the National Cancer Institute, Bethesda, MD. 相似文献