共查询到20条相似文献,搜索用时 335 毫秒
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Structural analysis of the mouse mdr1a (P-glycoprotein) promoter reveals the basis for differential transcript heterogeneity in multidrug-resistant J774.2 cells. 总被引:5,自引:2,他引:3 下载免费PDF全文
S I Hsu D Cohen L S Kirschner L Lothstein M Hartstein S B Horwitz 《Molecular and cellular biology》1990,10(7):3596-3606
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Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells 总被引:10,自引:4,他引:6 下载免费PDF全文
Y Inagaki Y Tsunokawa N Takebe H Nawa S Nakanishi M Terada T Sugimura 《Journal of virology》1988,62(5):1640-1646
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Expression in Spiroplasma citri of an epitope carried on the G fragment of the cytadhesin P1 gene from Mycoplasma pneumoniae. 总被引:7,自引:4,他引:3 下载免费PDF全文
We have previously described the use of the replicative form (RF) of Spiroplasma citri virus SpV1 as a vector for cloning and expressing foreign genes in S. citri, an organism which reads UGA as a tryptophan codon (C. Stamburski, J. Renaudin, and J.M. Bové, J. Bacteriol. 173:2225-2230, 1991). We now report cloning and expression in S. citri of the G fragment of cytadhesin P1 gene from Mycoplasma pneumoniae. The G fragment was inserted in the SpV1 RF downstream of a synthetic ribosome binding site and introduced into S. citri by electroporation. Northern (RNA) blot analyses showed that in S. citri, the G fragment was transcribed from an SpV1 RF promoter as a 1.2-kb mRNA. The translation product was detected by Western blotting (immunoblotting) with a rabbit antiserum raised against total proteins from M. pneumoniae (strain FH) and was proved to be P1 specific by using monoclonal antibodies specific for the G region of the P1 protein. The apparent molecular mass of the polypeptide (24.5 kDa) indicates that in S. citri, the G fragment was fully translated in spite of the seven UGA codons present in the reading frame. 相似文献
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