Experimental study of the plasma effect on the generation of microwave radiation in systems with a virtual cathode

Results are presented from experimental studies of the plasma effect on the generation of microwave radiation in systems with a virtual cathode. Using a triode with a virtual cathode as an example, it is shown that the cathode and anode plasmas reduce the generation efficiency; in particular, the power of the generated microwave radiation decreases and the radiation frequency and the microwave pulse duration change appreciably. It is demonstrated that, at high microwave powers, the power radiated into free space can be reduced by the plasma generated at the surface of the output window. This plasma appears due to discharges developing on the window surface under the combined action of bremsstrahlung, UV radiation, electrons and ions arriving from the beam formation zone, and the microwave electric field.     

The action of osmolytes on the destructive effect of ionizing radiation,hyperthermia, microwave radiation,and ultrasound

New data on the influence of osmolytes (NaCl, glycerol) on the destructive effect of ionizing radiation, hyperthermia, microwave radiation and ultrasound were obtained from experiments with bacterial cells. The pattern of manifestation of the osmolyte protective effect is presented. It was found that the protective effect of osmolytes from damaging factors (antagonistic interaction) can be detected only within a certain range of agent “doses.” Inside this range, there is an optimum providing the maximum protection. It was shown that, to provide the highest antagonistic effect with increased intensity of one of the agents, a corresponding change in the intensity of the other agent involved in the interaction is required. It is concluded that, in addition to DNA damage, membrane injury and osmotic homeostasis system may be considered as critical targets in cell destruction after the combined action of various environmental factors.     

Effect of pulse electromagnetic radiation on erythrocyte ghosts

The pulse microwave radiation has been shown to increase the fluorescence intensity of 2-toluidinonaphthanene-6-sulfonate (2,6-TNS) and 1-anilinonaphthalene-8-sulfonate (1,8-ANS) built-in membranes of erythrocyte ghosts. In experiments with 2,6-TNS a frequency dependence of the effect of microwave radiation with maximum within the frequency range of 55-65 Hz has been found. It is suggested that the changes registered with fluorescent probes are induced by mechanical oscillations generated by the pulse microwave radiation.     

Effect of a DC external electric field on the properties of a nonuniform microwave discharge in hydrogen at reduced pressures

The effect of a dc external electrical field on the properties of a highly nonuniform electrode microwave discharge in hydrogen at a pressure of 1 Torr was studied using optical emission spectroscopy and selfconsistent two-dimensional simulations. It is shown that the negative voltage applied to the antenna electrode with respect to the grounded chamber increases the discharge radiation intensity, while the positive voltage does not affect the discharge properties. The simulation results agree well with the experimental data.     

High-frequency surface waves at a plasma-metal interface: II. Dispersion of a nonlinear wave

A study is made of the dispersion properties of nonlinear surface waves propagating along a plasma-metal interface under conditions corresponding to the formation of a space charge sheath that equalizes the electron and ion fluxes to the wall. Oscillations of the plasma boundary under the action of the surface wave field are taken into account. It is shown that these oscillations are the main nonlinear mechanism for generating wave field harmonics and are analogous to the nonlinearity in the current-voltage characteristic of the space charge sheath. The effect of the nonlinearity on the dispersion properties of surface waves due to the relationship between the sheath thickness and wave amplitude is calculated with allowance for harmonic generation. The energy transported by surface waves under conditions typical of RF and microwave discharges is calculated.     

Ion-acoustic solitons in dusty plasma

The dynamics of dust ion-acoustic solitons is analyzed in a wide range of dusty plasma parameters. The cases of both a positive dust grain charge arising due to the photoelectric effect caused by intense electromagnetic radiation and a negative grain charge established in the absence of electromagnetic radiation are considered. The ranges of plasma parameters and Mach numbers in which ??conservative?? (nondissipative) solitons can exist are determined. It is shown that, in dusty plasma with negatively charged dust grains, both compression and rarefaction solitons can propagate, whereas in plasma with positively charged dust grains, only compression solitons can exist. The evolution of soliton-like compression and rarefaction perturbations is studied by numerically solving the hydrodynamic equations for ions and dust grains, as well as the equation for dust grain charging. The main dissipation mechanisms, such as grain charging, ion absorption by dust grains, momentum exchange between ions and dust grains, and ion-neutral collisions are taken into account. It is shown that the amplitudes of soliton-like compression and rarefaction perturbations decrease in the course of their evolution and their velocities (the Mach numbers) decrease monotonically in time. At any instant of time, the shape of an evolving soliton-like perturbation coincides with the shape of a conservative soliton corresponding to the current value of the Mach number. It is shown that, after the interaction between any types of soliton-like perturbations, their velocities and shapes are restored (with a certain phase shift) to those of the corresponding perturbations propagating without interaction; i.e., they are in fact weakly dissipative solitons.     

Formation of vortex dust structures in inhomogeneous gas-discharge plasmas

A generalized analytical model of instabilities in a dusty plasma with a nonzero grain charge gradient in a field of nonelectrostatic forces is considered. A review is given of different experimental observations of the dust self-oscillations that occur in the plasmas of an rf capacitive discharge and a dc glow discharge and whose appearance can be explained in terms of the proposed model. It is shown that the change in the grain charge gives rise to dynamic dust structures in laboratory gas-discharge plasmas. Attention is focused on the analysis of the onset of vortex motion of the dust grains.     

Spatial structure of emission from an electrode microwave discharge in hydrogen

The structure of electrode microwave (2.45 GHz) discharges in hydrogen with electrodes of various shapes and sizes at pressures of 1–8 torr and incident powers of 2–150 W is studied. It is found that the discharges exhibit a common feature that is independent of the antenna-electrode design: near the electrode surface, there is a thin bright sheath surrounded by a less bright, sharply bounded region, which is usually shaped like a sphere. It is suggested that the structure observed arises because the microwave field maintaining the discharge is strongly nonuniform. Near the electrode, there exists a thin dense plasma sheath with a high electron density gradient. A strong dependence of the electron-impact excitation coefficient on the electric field makes the effect even more pronounced. As the electron density decreases due to dissociative recombination, the microwave field gradient decreases and the discharge emission intensity tends to a nearly constant value. Presumably, in the boundary region of the discharge, there exists a surface wave, which increases the emission intensity at the periphery of the discharge.     

Heat exchange in dusty plasma

The effect of the charge of a dust grain on the exchange of its heat with plasma particles and with neutral gas particles in an anisotropic dusty plasma with dissipative flows is discussed. It is shown, in particular, that nonuniform heating of the grain surface gives rise to the radiometric force, which may be stronger than the ion wind force. Also, the grain charge causes the thermophoretic force to change its sign.     

Effect of weak combined low frequency constant and alternative magnetic fields on intrinsic fluorescence of proteins in aqueous solutions]

It was shown that weak combined static (42 microT) and low-frequency variable (40 nT; 3-5 Hz) magnetic fields change the intensity of intrinsic fluorescence of some proteins (cytochrome c, bovine serum albumin, horseradish peroxidase, alkaline phosphatase). The effect can be interpreted as a change in the conformational state of the protein in water environment by the action of weak magnetic fields. The dynamics of the process, the concentration dependence, the binding of proteins to the fluorescence probe 1,8-ANS after treatment with magnetic fields, the frequency dependence of these reactions, and the dependence of the effect on the presence of the static constituent of the magnetic field were studied. It was shown that the changes in the intrinsic fluorescence of some enzymes (horseradish peroxidase, alkaline phosphatase) are related to changes in their functional activity. It was found that the effect is partially transferred via a solvent (water, 0.01 M NaCl) preliminarily treated with magnetic field. In the solvent, changes in its intrinsic fluorescence by the action of weak magnetic fields were also registered.     

Changes in serum alkaline phosphatase activity during in vitro exposure to amplitude-modulated electromagnetic field of ultrahigh frequency (2375 MHz) in guinea pigs

The activity of alkaline phosphatase by the action of pulse-modulated microwave radiation was studied. The carrier frequency of radiation was 2375 MHz, the range of modulation pulse rate was 10-390 Hz with the on-off time ratio 2, and the specific absorption rate was 8 and 0.8 microW/cm2. Time of exposure was 1 and 3 min under conditions of continuous temperature control. It was shown that the activity of alkaline phosphatase depends on both modulation frequency and intensity of superhigh-frequency electromagnetic radiation. At a frequency of 70 Hz, the activity of alkaline phosphatases increased 1.8-2.0 times.     

Polarization bremsstrahlung from a fast structural ion in a plasma

Polarization bremsstrahlung from a fast hydrogen-like ion in a plasma is calculated and analyzed in the first Born approximation with allowance for the contribution from two radiation mechanisms (channels): (i) radiation from the conversion of the electromagnetic field of the ion into a real photon by plasma electrons and (ii) radiation from the virtual excitation of the bound electron of the ion. It is shown that the intensity of the polarization bremsstrahlung generated via the second channel is sharply peaked in narrow spectral-angular ranges around the eigenfrequencies of the electron core of the fast ion and, moreover, the spectral-angular intensity distribution depends strongly on the velocity of the incident particle. The dependence of both the polarization bremsstrahlung mechanisms on the plasma parameters is investigated.     

Two-step exposure of biological objects to infrared laser and microwave radiation]

The effect of two-step exposure of bacterial objects to infrared laser and microwave pulse radiations was studied. The effect is determined by the time interval between two excitation steps and pulse duration. It was shown that the biologically active dose of microwave radiation is much lower than that of infrared laser radiation; however, laser radiation induces a stronger cellular response. It was found that microwaves enhance the efficiency of infrared laser radiation.     

Effects of microwave radiation (340 and 900 MHz) on different structural levels of erythrocyte membranes

By use of fluorescence probes 1-anilinonaphthalene-8-sulfonic acid, 2-toluidinylnaphthalene-6-sulfonate, pyrene, perylene and chemical label phosphatidylethanolamine 2,4,6-trinitrobenzele sulfonic acid, the effect of microwave radiation on the erythrocyte membrane was studied. The studies with the fluorescence probes were carried out on erythrocyte ghosts and with 2,4,6-trinitrobenzene sulfonic acid on whole erythrocytes. The fluorescence was measured during irradiation of the membranes with 340-MHz microwaves at an SAR of 100 W/kg. Trinitrophenylation of phosphatidylethanolamine from whole erythrocytes was performed simultaneously with microwave irradiation at 900 MHz (10 mW/cm2). It was shown that the microwave field decreased lipid viscosity, altered the structural state of lipid-protein contact regions, and decreased the protein shielding of lipids. These changes corresponded to those produced by thermal action of microwaves.     

Perturbation of a collisional plasma by exterior bodies in a uniform external electric field

The charging of a metal sphere in a weakly ionized collisional plasma in a uniform external electric field is investigated with allowance for the effect of the space charge field and ionization-recombination processes. The sphere charge and the spatial distributions of charged plasma particles are calculated both numerically and analytically (for some particular cases) for the case of a strong external field. It is found that the size of the perturbed plasma region is determined by the external field and the intensity of recombination processes. It is shown that the total electric charge (the sphere charge plus the plasma space charge) is zero in accordance with predictions of the theory of static currents in a conducting medium.     

Mechanism of low-level microwave radiation effect on nervous system

The aim of this study is to explain the mechanism of the effect of low-level modulated microwave radiation on brain bioelectrical oscillations. The proposed model of excitation by low-level microwave radiation bases on the influence of water polarization on hydrogen bonding forces between water molecules, caused by this the enhancement of diffusion and consequences on neurotransmitters transit time and neuron resting potential. Modulated microwave radiation causes periodic alteration of the neurophysiologic parameters and parametric excitation of brain bioelectric oscillations. The experiments to detect logical outcome of the mechanism on physiological level were carried out on 15 human volunteers. The 450-MHz microwave radiation modulated at 7, 40 and 1000 Hz frequencies was applied at the field power density of 0.16 mW/cm². A relative change in the EEG power with and without radiation during 10 cycles was used as a quantitative measure. Experimental data demonstrated that modulated at 40 Hz microwave radiation enhanced EEG power in EEG alpha and beta frequency bands. No significant alterations were detected at 7 and 1000 Hz modulation frequencies. These results are in good agreement with the theory of parametric excitation of the brain bioelectric oscillations caused by the periodic alteration of neurophysiologic parameters and support the proposed mechanism. The proposed theoretical framework has been shown to predict the results of experimental study. The suggested mechanism, free of the restrictions related to field strength or time constant, is the first one providing explanation of low-level microwave radiation effects.     

Spontaneous electrical activity of the rat cerebral cortex during microwave irradiation

A study was made of changes in spontaneous electrical activity of rat brain cortex induced by a single exposure to microwave radiation (electromagnetic fields of 0.1, 1.0, 10, and 35 mW/cm2). The animals were exposed in anechoic chambers to continuous waves at 2450 MHz in conditions of continuous generation. The data obtained indicate that the EEG parameters change under the effect of microwave radiation. The technique applied permits to study the occurrence and development of the CNS reactions to microwave radiation at the time of action of the factor.     

Salt-assisted acid hydrolysis of chitosan to oligomers under microwave irradiation

The effect of inorganic salts such as sodium chloride on the hydrolysis of chitosan in a microwave field was investigated. While it is known that microwave heating is a convenient way to obtain a wide range of products of different molecular weights only by changing the reaction time and/or the radiation power, the addition of some inorganic salts was shown to effectively accelerate the degradation of chitosan under microwave irradiation. The molecular weight of the degraded chitosan obtained by microwave irradiation was considerably lower than that obtained by traditional heating. Moreover, the molecular weight of degraded chitosan obtained by microwave irradiation assisted under the conditions of added salt was considerably lower than that obtained by microwave irradiation without added salt. Furthermore, the effect of ionic strength of the added salts was not linked with the change of molecular weight. FTIR spectral analyses demonstrated that a significantly shorter time was required to obtain a satisfactory molecular weight by the microwave irradiation-assisted inorganic salt method than by microwave irradiation without inorganic salts and conventional technology.     

Charge and potential of a dust grain versus the intergrain distance and establishment of the latter in a low-pressure plasma

Results from experimental studies of ordered dust structures in plasma are reviewed. The experimental conditions and the data on the grain size and intergrain distance in plasma dust crystals are analyzed. It is shown that intergrain distance is a function of the grain size. The range of the ratio of the dust grain size to the Debye radius within which plasma dust crystals can form is determined. A volume cell surrounding a dust grain in plasma is considered. It is found that the potential and charge of the grain depend substantially on the intergrain distance. The charge, potential, and potential energy of a dust grain in a plasma dust crystal, as well as the electrostatic force exerted by the plasma field on the grain, are calculated by the method of molecular dynamics as functions of the intergrain distance. The corresponding analytic approximations and the criterion for the establishment of a steady-state intergrain distance are proposed.     

Low frequency amplitude modulated microwave fields change calcium efflux rates from synaptosomes

Calcium(⁴⁵Ca^{2 +}) efflux from preloaded synaptosomes was studied with a continuous perfusion technique and the rate constants of a two-phase efflux process calculated. When 16-Hz sinusoidally amplitude modulated 450-MHz microwave field (maximal incident intensity 0.5 mW/ cm², modulation depth 75%) was applied during the second phase, the rate constant increased by 38%. Unmodulated or 60-Hz modulated signals were not effective. This microwave fieldinduced change can be distinguished from CaCl₂-stimulated ⁴⁵Ca^{2 +} efflux which is most probably derived intracellularly. These data suggest that the microwave-field-induced change in calcium efflux probably did not involve intracellular calcium. Also, this change in the dynamic property of synaptosomes did not require gross anatomically intact tissue as a substrate for field tissue interaction.