共查询到20条相似文献,搜索用时 15 毫秒
1.
J. R. Caradus A. D. Mackay S. Wewala J. Dunlop A. Hart J. Van Den Bosch M. G. Lambert M. J. M. Hay 《Plant and Soil》1992,146(1-2):199-208
Genotypes of white clover that exhibited divergent responses to P were identified in a glasshouse pot trial. Six high P-responding genotypes were selected from previously identified high P-responding cultivars and 5 low P-responding genotypes were selected from previously identified low P-responding cultivars. These were crossed in a full diallel design without selfing and reciprocals were kept separate. The P-response of progeny lines was compared with parents. High P-response was dominant over low P-response with progeny from crosses between high and low P-response genotypes being similar to the high P-response parent. Reciprocal effects were not significant. The general combining abilities of high P-response genotypes were generally greater than that of the low P-response genotypes, although there were significant specific combining abilities. Narrow sense heritabilities for P response were moderate, 0.46 based on the linear coefficient and 0.33 based on the quadratic coefficient of the fitted response curves.The mode of inheritance, feasibility of manipulating differences in P response by breeding and future directions of this work are discussed.Deceased.Deceased. 相似文献
2.
Summary Sodium nitrate applications ranging from 0.36 to 22.84 mM N were shown to depress rates of nodule formation and reduce total nitrogen fixation (acetylene reduction) in white clover plants grown in aseptic test tube culture.Low nitrate levels gave an initial depression in symbiotic activity but the reduction was of short duration and these treatments were subsequently associated with enhanced rates of nodule formation and nitrogen fixation. As a result, phenotypic variation appeared to be strongly differentially affected by the amount of nitrate present. A subsequent experiment suggested that much of the variation was a consequence of early enhancement of plant growth rates by low levels of nitrate followed by rapid depletion thus giving a transitory inhibitory effect. This was confirmed in a third experiment in which the range of nitrate concentration was held constant. Differential effects on variability in nodule formation and nitrogen fixation were then greatly reduced but there was still a residual level of plant-to-plant variation. The results have clear implications for selecting genetic variants capable of fixing di-nitrogen in the presence of combined N. The provision of a single limiting dose of combined nitrogen to a population containing individuals with inherently different growth rates can bring about variations in the phenotypic expression of symbiotic characters. These variations are unlikely to be based on genetic factors which have a direct and stable effect on nodule development and nitrogenase activity. The implications of the results for plant breeding are discussed. 相似文献
3.
An aluminium (Al) tolerant genotype of white clover was compared with an Al susceptible genotype in artificial soil profiles in which exchangeable Al increased with depth. The tolerant genotype had a greater proportion of its root mass deeper in the soil than the susceptible genotype. Nitrogenase activity showed a similar pattern. Shoot Al concentration did not vary between the genotypes but root Al in the susceptible line was twice that in the tolerant genotype. Plant potassium content in the susceptible line was relatively less, probably in response to higher aluminium content. 相似文献
4.
Influence of boron on the reproductive growth of white clover (Trifolium repens L.) cultivars 总被引:1,自引:0,他引:1
The effect of boron on the reproductive growth of four contrasting white clover cultivars was examined in a controlled environment and glasshouse experiment. Plants grown under a nutrient regime excluding boron and those receiving 0.5 mg/litre boron had fewer stolons, reproductive stolons and inflorescences than those receiving 1, 2 or 3 mg/litre. Inflorescences were also smaller and had fewer seeds per floret. A boron concentration of 1 mg/litre gave optimum reproductive growth, higher levels of boron giving no further increase. Peduncle length and nectar quantity, but not nectar quality, were also influenced by boron indicating a possible beneficial effect of this element on pollinator activity. Cultivars differed in both vegetative and reproductive growth. Cv. Gwenda, a small leaved cultivar, had more stolons but fewer inflorescences than cv. Menna and smaller inflorescences than cv. Olwen, a large leaved cultivar. Generally, these cultivars responded similarly to boron. The implications of these results for the reproductive growth of white clover are discussed. 相似文献
5.
The combined effects of soil water deficit and above and below ground interspecific plant competition on the growth, water-use
efficiency (WUE), and measured carbon isotopic composition (δ13C) values of white clover and ryegrass were studied. White clover and ryegrass were grown in specially designed crates 1)
individually; 2) in shoot competition; or 3) in shoot + root competition and either well-watered or at a moderate or severe
soil water deficit. The effects of shoot + root competition on shoot dry matter growth were substantial and benefited both
white clover and ryegrass when well-watered or at a moderate soil water deficit, while severely reducing white clover shoot
dry matter growth at severe soil water deficit. Plant competition did not affect the WUE of white clover or ryegrass. As soil
water deficit increased, the WUE of white clover did not change whereas the WUE of ryegrass increased and was greater than
that of white clover. This was attributed to the lower leaf water conductance of ryegrass which conserved water and maintained
growth longer compared to white clover. A stronger correlation existed between soil water deficit and measured δ13C values for ryegrass at each plant competition level (P<0.001) than existed for white clover (individual: P<0.01; shoot + root: P<0.001; shoot: P<0.10). Unlike white clover, the relationship between measured δ13C values and shoot dry matter growth indicated that C assimilation for ryegrass was dependent on type of plant competition.
That WUE remained constant for white clover while measured δ13C values increased as soil water deficit increased, suggests that the role below ground respiration rate played in determining
δ13C values increased. The WUE of white clover appears to be independent of the nature of the competition between plants and
the soil water deficit level at which it is grown, whereas for ryegrass, the addition of root competition to shoot competition
should lead to increases in its WUE.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
J. R. Caradus 《Plant and Soil》1992,146(1-2):209-217
Ninety eight white clover genotypes were cloned and grown in pots at two levels of phosphorus (P) supply in soil. After harvest the nitrogen (N) and P content of shoot (leaf, petiole and unrooted stolon), stolon and root tissue was determined. Broad sense heritabilities for %N, %P, and proportion of total N or P in each tissue type were calculated. Heritabilities ranged from 0.22 to 0.68. They were generally higher for %P than %N; and higher in shoot and stolon tissue than root tissue for %P, %N, and proportion of N or P. Level of P in which plants were grown had little effect on heritability values. Genotypes from bred cultivars differed from those collected from hill country pastures for plant size, and partitioning of N and P to shoot, stolon and root. Relationships between plant characters were examined to determine the consequences of selection. 相似文献
7.
8.
Plants of white clover ( Trifolium repens L.) cv. Olwen were grown in an open glasshouse maintained at a mean temperature of 20o C and ovule growth and seed production measured. Differences in the rate of growth of ovules within ovaries were observed as early as 2 days after pollination. Ovules reached a maximum size after 8 days with the smallest only half the size of the largest. After 8 days, the smallest ovules became flaccid and shrivelled. Ovule position within the ovary had little effect on the frequency of seed set and although there was an apparently higher probability that central ovules produced a seed than those nearer the peduncle or style this was not statistically significant. Inflorescence position and floret position on the inflorescence had a significant effect on the number of seeds per floret and seed weight; the first formed inflorescences and the first florets to be pollinated on each inflorescence had more seeds per floret and heavier seeds and fewer florets with no seed than later pollinated florets. There were also differences between florets within the same whorl. The role of a number of factors which may influence floret site utilisation are discussed. 相似文献
9.
RICHARD A. ENNOS 《Biological journal of the Linnean Society. Linnean Society of London》1981,15(1):75-82
Seed populations of white clover polymorphic for the presence/absence of both ovariogenic glucosides and the hydrolysing enzyme linamarase, were introduced into three natural populations. Over the first six months of life a significant increase in the frequency of linamarase containing individuals occurred. Estimated selection coefficients against plants lacking linamarase were in the region of 0.3. This result may have been due to selection at the enzyme locus alone, or to selection favouring cyanogenic individuals which possess both cyanogenic glucosides and enzyme. 相似文献
10.
Summary Humidity, at the young nodes of white clover stolones, varied by enclosing nodes in the atmosphere above a range of saturated solutions, inhibited root initiation at 85% RH or less. The threshold humidity for root initiation increased to about 93% on young nodes subject to moisture stress or old nodes on well watered plants in which root initiation had been previously suppressed by low humidity.Roots at old nodes and at the three youngest on stolons were either subject to moisture stress or adequately watered. Growth of young roots and N2-fixation were more adversely affected by the direct effects of drought than by subjecting old roots to drought. Although old roots under stress affected new root growth and N2-fixation, length of roots and lateral root number were little affected. By contrast stolon growth was affected more by stress to old roots than to young nodes, although after 6 weeks the contribution made by young roots to stolon growth was almost as high as old roots.The data suggest that deep roots at old nodes will allow clover stolons to grow during drought due to the high acropetal movement of water but initiation of roots and functioning of young roots at the soil surface will be adversely affected, with possible implications on the persistence of clover. 相似文献
11.
Flowers of white clover (Trifolium repens L.) are hermaphrodite and self-incompatible; their cross-pollination depends entirely on insect visitors, mainly bees (Apoidea).
Because self-pollination of white clover occurs before flower anthesis, we determined whether selfing affected the pollination
efficiency of a honeybee visit. We compared pollen deposition in emasculated and intact flowers following (1) a single honeybee
visit, (2) open-pollination for a day and (3) enclosure in a cloth bag to prevent insect visits. In emasculated flowers, open-pollination
resulted in more pollen deposited than after one visit (+30%) which is consistent with flowers being visited more than once
by pollinators during the course of a day. On intact flowers, saturation of the stigma was achieved after the first visit
of a honeybee (near 280 grains) because of self-pollination. Additional visits did not increase pollen deposits, but they
improved pollen efficiency in terms of numbers of pollen tubes reaching the ovules. In such a context of easily saturated
stigmas, self-pollen does not inhibit cross-pollen activity, but represents a constraint for pollination which demands multiple
bee visits to each flower to achieve maximum fertilization.
Received: 20 May 1997 / Accepted: 25 October 1997 相似文献
12.
Summary Plants of white clover (Trifolium repens) were grown under canopies of clover leaves floating on shallow glass tanks of water and their growth was compared with that of plants under canopies of black polythene leaves. The experimental design allowed the growth of the clover plants in canopy filtered light to be compared with that in unfiltered light at different intensities of Photosynthetically Active Radiation (P.A.R.). The effect of canopy filtered light was to exaggerate the effects of reduced P.A.R. especially in promoting petiole extension and inhibiting stolon branching. Two clones of white clover differed in their responses to P.A.R. and to light quality and there were significant interactions between the effects of the intensity of P.A.R. and type of shade. It is argued that it may be important for both ecologists and plant breeders to recognise the role of radiation quality in regulating the dynamics of pasture. 相似文献
13.
A putative contribution of polyamines to the control of peptidase activity expression during re-growth was studied in source organs (roots and stolons) of defoliated white clover (Trifolium repens L.). Endopeptidase activity increased in roots during the first 6 days following complete defoliation, while exopeptidase expression seemed to be restricted to the early hours of re-growth. These changes correlated with an immediate 80% decline in the content of total free polyamines, mainly represented by the diamine cadaverine. The inhibitory capacities of cadaverine and spermine were tested on enzyme activity in vitro in order to elucidate whether the endogenous polyamine level was associated with the cut-induced endopeptidase expression. Cadaverine seemed to inhibit endopeptidase activity of stolons but not root endopeptidase activity. These data support the view that polyamines may play a role in the regulation of peptidase expression in source organs of white clover during post-clipping re-growth. The existence of different endopeptidase isoforms in roots and stolons is discussed in relation to the molecular mechanisms by which polyamines may regulate their activities.Abbreviations AP
aminopeptidase
- Cad
cadaverine
- CP
carboxypeptidase
- EP
endopeptidase
- PA(s)
polyamine(s)
- Spm
spermine 相似文献
14.
W. A. Parrott 《Plant cell reports》1991,10(1):17-21
Cotyledons from immature embryos of white clover (Trifolium repens L.) cv. Osceola were exposed to 2,4-D or NAA to induce somatic embryogenesis. NAA at 10 or 20 mg 1–1 was very inefficient at stimulating embryogenesis, while concentrations of 30 or 40 mg 1–1 resulted in death of the explant tissue. Continuous exposure of cotyledons to 40 mg 1–1 2,4-D resulted in somatic embryos which were arrested at the globular stage, or which underwent cycles of secondary embryogenesis, never proceeding beyond the globular stage. A 10 day exposure time to 2,4-D at the same concentration led to formation of somatic embryos, most of which had poorly developed cotyledons. Almost 10% of the somatic embryos converted into plants following transfer to medium devoid of growth regulators. Attempts to improve morphology of somatic embryos by using shorter exposure times to 2,4-D at 40 mg 1–1, or by maintaining the 10 day exposure time while varying the concentration of 2,4-D, were not successful. Plants were obtained from all parents evaluated, although at different frequencies. 相似文献
15.
Two divergent populations of T. repens cv. Haifa developed from two generations of recurrent selection for shoot chloride concentration, were grown in the greenhouse at 0 and 40 mol m–3 NaCl. Over two harvest cycles at 40 mol m–3 NaCl, the population selected for a low concentration of chloride in the shoot maintained a significantly lower chloride and sodium concentration compared with those plants selected for a high shoot chloride concentration. The distribution of chloride in the shoots was further examined in a subsample of plants from both populations. In all plants, concentrations of chloride were lower in the expanding and fully expanded leaves than in the older leaf tissue or petioles.While there were no significant differences in the photosynthetic rates between lines, shoot yields and relative leaf expansion rates were higher in the low chloride population. Plant death was greater in plants selected for high shoot chloride. These results suggest that selections based on measurements of low shoot chloride concentrations may be successful in developing a cultivar of T. repens with improved salt tolerance. 相似文献
16.
Effects of polyethylene glycol (PEG)-induced water stress on the activities of total leaf superoxide dismutase (SOD) and chloroplast
SOD (including thylakoid-bound SOD and stroma SOD) are described in white clover (Trifolium repens L.) grown in solution culture from rooted cuttings. Both leaf SOD and chloroplast SOD activities were markedly enhanced with
increasing concentration of PEG stress, generating osmotic potentials around the roots 0, −0.5, −1.0, −1.5 MPa. The effects
increased with time up to 72 h. Chloroplast Fe-containing SOD represented about 30% of the total leaf SOD activity in the
control plants and a significant increase in chloroplast SOD activity was found during the stress period. This accounted for
about 35.5–71.1% of the total leaf SOD activity. The proportion of chloroplast SOD in total leaf SOD not only increased with
the decreasing of osmotic potential, but also increased with incubation time. Furthermore, the increase in thylakoid-bound
SOD activity was much higher than that of stroma SOD in chloroplast of plants under water stress. The enhanced chloroplastic
SOD activity, especially thylakoid-bound SOD activity, demonstrated in Trifolium repens suggests that Fe-SOD located in chloroplasts play a more important role than cytosolic Cu/Zn-containing SODs in scavenging
O2
−. 相似文献
17.
Three experiments are reported which examine the relative roles of host and Rhizobium genotypes as factors limiting clover (Trifolium repens L.) growth at low soil temperatures.In the first experiment un-nodulated clover and perennial ryegrass (Lolium perenne L.) were grown with non-limiting nitrate at root temperatures of 8, 10 and 12°C. The ryegrass had substantially better relative growth rates (RGR) than the clover with the biggest difference occurring at 8°C. Alterations in growth rate with temperature were more marked in clover than in ryegrass but the latter still produced several times more dry matter than clover at each temperature.In the subsequent experiments clover nodulated with different strains of rhizobia was grown with and without non-limiting additions of nitrate at root temperatures of 9, 12 and 15°C. Plants receiving nitrate generally produced more dry matter than those dependent upon Rhizobium for nitrogen but differences in yield between these treatments did not alter with temperature. This suggests that limitations imposed by nitrogen fixation are similar at both high and low temperatures. Indeed, there was some evidence that nitrogen limitations were rather more pronounced at the highest temperature. The first experiment clearly demonstrated that the clover genotype makes particularly poor use of nitrate at low root temperatures when compared to its common companion perennial ryegrass.It can be concluded that improvements in spring growth of clover will rest largely with alterations to the plant genotype and its ability to use combined nitrogen for growth at lower temperatures rather than with changes in rhizobia or any symbiotic characters. 相似文献
18.
The ability of white clover (Trifolium repens L.) to undergo cold acclimation is an important determinant of its persistence in mixed swards since growth rate at low temperatures sustains higher clover contents at the start of spring. During a re-growth period following defoliation, a gradual exposure of the root system (cv. Grasslands Huia) led to some physiological and morphological changes of cold-adaptive significance, similar to those developed by clover ecotypes originating in northern areas of Europe. Thus, cold exposure of the root system resulted in small-leaved prostrate forms of white clover after one month of re-growth. Similarly, cold exposure increased the ability of plants to store nitrogen since the application of low temperatures to the root system enhanced soluble protein accumulation in roots and in stolons. More specifically, cold exposure of the roots induced gene expression of a vegetative storage protein (17.3 kDa VSP) in both organs. These results demonstrate that the root system of clover plants should be a site of perception of the low-temperature stimulus, and gave rise to the question of the transduction of the cold signal from the roots to the aerial parts. On the basis of this study and taking into account molecular aspects concerning the clover VSP, it is suggested that this protein could participate in cold acclimation in addition to its role in nitrogen storage. 相似文献
19.
Effects of photoperiod and temperature on vegetative growth of seedling populations and clones of white clover ( Trifolium repens L.) originating from various latitudes (58°48'–69°54'N) and altitudes (up to 1100 m above sea level) were studied in a phytotron. Dry matter production, stolon elongation, petiole elongation and leaf lamina size were enhanced by long photoperiod. The requirement for long photoperiod increased with decreasing temperature. At 6°C the maximum growth was recorded under 24-h daylength. At 18°C already an increase in photoperiod from 12 to 15 h significantly enhanced growth, and maximum growth was obtained at 21-h photoperiod. The studied populations responded similarly to daylength, and the results did not indicate photoperiodic ecotypes in the material. The southernmost clones and populations generally had the highest dry matter production at all temperature treatments (6–18°C). Variation between clones within one location was, however, significant, and rapidly growing clones were found also in high-latitude locations. Dry matter production was poorly correlated with the morphological characters observed, but in some cases significant correlation with leaf lamina size was found. 相似文献
20.
The 15N isotope dilution technique and the N difference method were used to estimate N2 fixation by clover growing in a mixture with ryegrass, in a field experiment and a controlled environment experiment. Values obtained using N difference were approximately 25% lower than those estimated using 15N isotope dilution. In the field experiment there was a measured N benefit to grass growing with clover, equivalent to 42.7 kgN ha-1. The grass in the mixture had a lower atom %15N content and a higher N content than grass in a monoculture; therefore values for N2 fixation were different depending on choice of control plant i.e. monoculture or mixture grass. In the controlled environment experiment there were no significant differences between either the atom %15N contents or the N contents of monoculture grass and grass growing in a mixture with clover. It is concluded that there is a long term indirect transfer of N from clover to associated grass which can lead to errors in estimates of N2 fixation. 相似文献