共查询到20条相似文献,搜索用时 20 毫秒
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Fuks F Hurd PJ Wolf D Nan X Bird AP Kouzarides T 《The Journal of biological chemistry》2003,278(6):4035-4040
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Methylation of the cyclin A1 promoter correlates with gene silencing in somatic cell lines, while tissue-specific expression of cyclin A1 is methylation independent 下载免费PDF全文
Müller C Readhead C Diederichs S Idos G Yang R Tidow N Serve H Berdel WE Koeffler HP 《Molecular and cellular biology》2000,20(9):3316-3329
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M L Mello A F Chambers B C Vidal W Planding U Schenck 《Analytical cellular pathology》2000,20(4):163-171
Increased amounts of chromatin condensation (i.e., localized areas of high DNA density, or chromatin higher order packing state) have been described in NIH 3T3 cells transformed with the Ha-ras oncogene. The structural basis for this oncogene-mediated alteration in nuclear organization is unknown. Since DNA methylation is likely to be involved in regulating the nucleosomal level of DNA packaging, we studied the role of DNA methylation in higher-order chromatin organization induced by Ha-ras. CpG-methylated DNA content was estimated in "condensed" chromatin of Ha-ras-transformed NIH 3T3 cell lines which differ in ras expression and ras-induced metastatic ability but present approximately the same values of "condensed" chromatin areas. The question posed was that if DNA methylation were involved with the chromatin higher-order organization induced by Ha-ras in these cell lines, the methylated DNA density in the "condensed" chromatin would also be the same. The DNA evaluation was performed by video image analysis in Feulgen-stained cells previously subjected to treatment with Msp I and Hpa II restriction enzymes, which distinguish between methylated and non-methylated DNA. The amount of methylated CpG sequences not digested by Hpa II in "condensed" chromatin regions was found to vary in the studied ras-transformed cell lines. DNA CpG methylation status is thus suggested not to be involved with the higher order chromatin condensation induced by ras transformation in the mentioned NIH 3T3 cell lines. 相似文献
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Rakesh Singal Shou Zhen Wang Thanh Sargent Sheng Zu Zhu Gordon D Ginder 《The Journal of biological chemistry》2002,277(3):1897-1905
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DNA methylation affects the formation of active chromatin 总被引:88,自引:0,他引:88