Experiments on the implosion of heterogeneous wire arrays on the S-300 facility

Results are presented from experiments on the implosion of simple and nested wire arrays of different mass and material composition (W and/or Al). The experiments were performed on the S-300 facility (a high-current pulsed power generator with a voltage pulse amplitude of 700 kV, current amplitude of 2.5–3.5 MA, and pulse duration of 100 ns) at the Kurchatov Institute (Moscow). The imploding arrays were recorded using five-frame laser shadowgraphy, three-frame image-tube photography, an optical streak camera, X-ray pinhole cameras with different filters, X-ray polychromator, and X-ray spectrometer on the basis of a convex mica crystal. Laser probing measurements indicate that the current-carrying structure undergoes a fast (over a time shorter than 10 ns) global rearrangement, which manifests itself as the emergence of transparent regions. This effect is presumably related to the grouping of the wires, which carry currents of a few tens of kiloamperes, or to the current filamentation in their common plasma corona. The radiation of liners of different chemical composition in the final compressed state has been investigated. Electric measurements performed in experiments with nested arrays (e.g., with an aluminum outer liner and a tungsten inner liner) indicate that the inner array, which is still at rest, intercepts the electric current from the outer array when the latter penetrates through it. The effect of the “fall” of the outer liner through the inner one in the course of magnetic implosion has been revealed for the first time by analyzing X-ray emission spectra.     

Conditional filamentation as an adaptive trait of bacteria and its ecological significance in soils

Bacteria can regulate cell morphology in response to environmental conditions, altering their physiological and metabolic characteristics to improve survival. Conditional filamentation, in which cells suspend division while continuing lateral growth, is a strategy with a range of adaptive benefits. Here, we review the causes and consequences of conditional filamentation with respect to bacterial physiology, ecology and evolution. We describe four major benefits from conditional filamentation: stress tolerance, surface colonization, gradient spanning and the facilitation of biotic interactions. Adopting a filamentous growth habit involves fitness trade-offs which are also examined. We focus on the role of conditional filamentation in soil habitats, where filamentous morphotypes are highly prevalent and where environmental heterogeneity can benefit a conditional response. To illustrate the use of information presented in our review, we tested the conditions regulating filamentation by the forest soil isolate Paraburkholderia elongata 5N^T. Filamentation by P. elongata was induced at elevated phosphate concentrations, and was associated with the accumulation of intracellular polyphosphate, highlighting the role of filamentation in a phosphate-solubilizing bacterium. Conditional filamentation enables bacteria to optimize their growth and metabolism in environments that are highly variable, a trait that can impact succession, symbioses, and biogeochemistry in soil environments.     

Excitation of magnetic fields by a circularly polarized laser pulse in a plasma channel

The excitation of quasistatic magnetic fields by a circularly polarized laser pulse in a plasma channel is considered. It is shown that, to second order in the amplitude of the electric field of the laser pulse, circular rotation of the plane of polarization of the laser radiation in a radially nonuniform plasma gives rise to a nonlinear azimuthal current and leads to the excitation of the radial and axial components of the magnetic field. The dependence of the magnetic field distribution over the plasma channel on the spatial dimensions of the pulse and on the channel width is investigated for a moderate-power laser pulse. The structure of the magnetic fields excited by a relativistic laser pulse in a wide plasma channel is analyzed.     

A molecular dynamics investigation into the mechanisms of material removal and subsurface damage of nanoscale high speed laser-assisted machining

Molecular dynamics is employed to study the mechanism of material removal and subsurface damage of monocrystalline silicon when it is under a nanoscale high-speed laser-assisted grinding of a diamond tip. Laser-assisted machining (LAM) is that the workpiece is locally heated by an intense laser beam before material removal. The effects of laser moving speed, laser pulse intensity and laser spot radius are thoroughly investigated in terms of atomic trajectories, phase transformation, temperature distribution, grinding temperature, grinding force and friction coefficient. The investigation shows that a higher laser moving speed reduces the subsurface damage and improves the material remove rate because of fewer atoms with five and six coordination atoms and more chips. Besides, both tangential grinding force (Fx) and normal grinding force (Fy) decrease as the laser moving speed increases. The distribution of high-temperature zone strongly depends upon the effect of laser pulse intensity and laser spot radius. Larger laser pulse intensity can make the material more fully softened before being removed. Moreover, as the laser pulse intensity becomes larger, the friction coefficients became smaller, the material remove rate improves and the depth of grinding increases. However, larger laser pulse intensity may result in a larger thermal deformation of workpiece. A larger laser spot radius reduces the grinding depth but increases the width of laser irradiation zone on machined surface. Thus, a suitable laser spot radius can improve the material removal rate. These results indicate that it is possible to control and adjust the laser parameters according to laser moving speed, laser pulse intensity and laser spot radius, and it provides a potential technology to improve a surface integrity and a smoothness of ground surface.     

Vacuum electron acceleration by a tightly focused,radially polarized,relativistically strong laser pulse

A test particle approach is used to solve the problem of direct electron acceleration by a short, intense, radially polarized laser pulse the focal spot diameter of which can be on the order of the laser wavelength. The fields of a tightly focused laser beam are described in terms of the Stratton-Chu integrals, with which to investigate electron acceleration when the paraxial approximation is inapplicable to laser fields. The dynamics of electron motion in a radially polarized, relativistically strong laser field is analyzed depending on the initial position of an electron in the focal region of the laser beam. The properties of the generated jets of accelerated electrons are investigated depending on the tightness of laser pulse focusing. Possible advantages of using radially polarized laser pulses for charged particle acceleration, as opposed to the use of linearly polarized ones, are discussed.     

强激光作用下生物组织光致破裂效应的研究

基于激光与生物组织作用时产生的光致破裂效应,利用脉冲激光直接聚焦于孕囊内的胚胎内部,激光产生的光致破裂效应对胚胎心脏造成损伤,造成胚胎的快速死亡,初步证明利用激光进行减胎手术有望成为一种新方法。采用输出波长1 064 nm、脉宽8 ns、单脉冲能量60～96 mJ可调的脉冲激光作用于牛肌肉组织样品,并用光学相干层析成像(OCT)系统测量实验样品的损伤直径和损伤深度,建立了不同的激光参数与被作用样品的损伤直径及损伤深度之间的关系。得出结论:样品的损伤深度和损伤直径与激光的脉冲能量成正相关关系,而与激光的脉冲频率成负相关关系。这对于激光应用于减胎手术时参数的优化和精确控制起到一定的指导作用。     

Cyclic AMP-protein kinase A and Snf1 signaling mechanisms underlie the superior potency of sucrose for induction of filamentation in Saccharomyces cerevisiae

Under specific environmental conditions, the yeast Saccharomyces cerevisiae can undergo a morphological switch to a pseudohyphal growth pattern. Pseudohyphal differentiation is generally studied upon induction by nitrogen limitation in the presence of glucose. It is known to be controlled by several signaling pathways, including mitogen-activated protein kinase, cyclic AMP-protein kinase A (cAMP-PKA), and Snf1 kinase pathways. We show that the alpha-glucoside sugars maltose and maltotriose, and especially sucrose, are more potent inducers of filamentation than glucose. Sucrose even induces filamentation in nitrogen-rich media and in the mep2Δ/mep2Δ ammonium permease mutant on ammonium-limiting medium. We demonstrate that glucose also inhibits filamentation by means of a pathway parallel to the cAMP-PKA pathway. Deletion of HXK2 shifted the pseudohyphal growth pattern on glucose to that of sucrose, while deletion of SNF4 abrogated filamentation on both sugars, indicating a negative role of glucose repression and a positive role for Snf1 activity in the control of filamentation. In all strains and in all media, sucrose induction of filamentation is greatly diminished by deletion of the sucrose/glucose-sensing G-protein-coupled receptor Gpr1, whereas it has no effect on induction by maltose and maltotriose. The competence of alpha-glucoside sugars to induce filamentation is reflected in the increased expression of the cell surface flocculin gene FLO11. In addition, sucrose is the only alpha-glucoside sugar capable of rapidly inducing FLO11 expression in a Gpr1-dependent manner, reflecting the sensitivity of Gpr1 for this sugar and its involvement in rapid sucrose signaling. Our study identifies sucrose as the most potent nutrient inducer of pseudohyphal growth and shows that glucose inactivation of Snf1 kinase signaling is responsible for the lower potency of glucose.     

Involvement of Candida albicans pyruvate dehydrogenase complex protein X (Pdx1) in filamentation

For 50 years, physiologic studies in Candida albicans have associated fermentation with filamentation and respiration with yeast morphology. Analysis of the mitochondrial proteome of a C. albicans NDH51 mutant, known to be defective in filamentation, identified increased expression of several proteins in the respiratory pathway. Most notable was a 15-fold increase in pyruvate dehydrogenase complex protein X (Pdx1), an essential component of the pyruvate dehydrogenase complex. In basal salts medium with < or = 100 mM glucose as carbon source, two independent pdx1 mutants displayed a filamentation defect identical to ndh51; reintegration of one PDX1 allele restored filamentation. Concentrations of glucose < or = 100 mM did not correct the filamentation defect. Expanding on previous work, these studies suggest that increased expression of proteins extraneous to the electron transport chain compensates for defects in the respiratory pathway to maintain yeast morphology. Mitochondrial proteomics can aid in the identification of C. albicans genes not previously implicated in filamentation.     

Involvement of cyclic AMP and its receptor protein in filamentation of an Escherichia coli fic mutant

Cyclic AMP (cAMP) inhibited septum formation in Escherichia coli PA3092 and induced cell filamentation at elevated temperatures. This phenomenon was first observed in E. coli PA3092 and is due to a temperature-sensitive mutation. We tentatively named this mutation fic (filamentation induced by cAMP). The fic gene was located near rpsL (formerly strA) on the E. coli K-12 map. the inhibitory effect of cAMP on cell division and filamentation in a fic mutant was not observed in a crp mutant. When cAMP was removed from the culture medium, filaments were divided into rods as the intracellular cAMP level decreased. These results suggest that the cAMP-cAMP receptor protein complex causes filamentation in the fic mutant, E. coli PA3092.     

Comparison of chemiluminescence from luminol solution and luminol–TiO2 suspension after illumination of a 355 nm pulse laser

Chemiluminescence (CL) from luminol solution and luminol–TiO₂ suspension after illumination of a 355 nm pulse laser is compared. Both the CL systems showed the CL spectra with maximum wavelength of 430 nm, suggesting that the emission was from the excite state of 3‐aminophthalate ion. The TiO₂ photocatalytically induced luminol CL could be separately detected either when the pulse laser power was smaller than 0.15 mJ/pulse or a slit was placed beyond ?2–2 mm in the vertical direction of the laser beam. The TiO₂ photocatalytically induced luminol CL intensity was linear to the laser power, while that of the 355 nm pulse laser‐induced was nonlinear. A log–log plot between the 355 nm pulse laser‐induced luminol CL intensity and laser power showed a near‐linear regression fit with a slope of 2.11, suggesting that a two‐photon absorption process of luminol was present in the 355 nm pulse laser‐induced luminol CL. Adsorbed oxygen on the surface of TiO₂ seemed to greatly contribute to the photocatalytically induced CL. Copyright © 2010 John Wiley & Sons, Ltd.     

Feasibility of stabilizing a vacuum-diode X-ray source with a laser-plasma cathode

Results are presented from experimental studies of discharge instabilities and the energy and temporal characteristics of a vacuum-diode X-ray source with a laser plasma cathode over a wide range of energies, intensities, and durations of the plasma-forming laser pulse. It is experimentally shown that the vacuum-discharge dynamics and radiation processes in different discharge stages substantially depend on the parameters of the laser radiation. The shortest recorded pulse duration (10 ns) of Ti K-line radiation (4.5 keV) with a total photon number of 10¹¹ is achieved when the laser plasma cathode is produced by a laser pulse with a duration of 27 ps and an intensity of 10¹³ W/cm². It is found that the contrast of characteristic emission against the bremsstrahlung background is maximum when discharge instabilities are suppressed and the accelerating voltage is three to four times higher than the threshold voltage for line excitation.     

Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli.

Certain Escherichia coli strains were shown to possess a novel system of cell division inhibition, called the SfiC+ phenotype. SfiC+ filamentation had a number of properties similar to those of sfiA-dependent division inhibition previously described: (i) both are associated with the SOS response induced by expression of the recA(Tif) mutation, (ii) both are associated with cell death, (iii) both are amplified in mutants lacking the Lon protease, and (iv) both are suppressed by sfiB mutations. SfiC+ filamentation and sfiA-dependent division inhibition differed in (i) the physiological conditions under which loss of viability is observed, (ii) the extent of amplification in lon mutants, (iii) their genetic regulation (SfiC+ filamentation is not under direct negative control of the LexA repressor), and (iv) their genetic determinants (SfiC+ filamentation depends on a locus, sfiC+, near 28 min on the E. coli map and distinct from sfiA).     

Deletion of the Candida albicans G-protein-coupled receptor, encoded by orf19.1944 and its allele orf19.9499, produces mutants defective in filamentous growth

Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The protein is 20.9% identical to the Saccharomyces cerevisiae Gpr1p receptor that signals both glucose availability and nitrogen limitation. Deletion of both copies of the gene in C. albicans abolished filamentation by colonies embedded in rich media (YPS, YPGal, and YPGlu), whereas mutants carrying a single copy of the gene were indistinguishable from the parental strain under these conditions. On medium containing low concentrations of ammonia (SLAD and SLAM media), surface colonies of both the homozygous deletion mutants and the mutants carrying a single copy of the gene were defective in filamentation. Serum-induced germ tube formation was unaffected by deletion of this gene, as was filamentation of the mutants growing on the surface of solid Spider medium at 37 degrees C or embedded in solid Spider medium at 25 degrees C. The protein encoded by orf19.1944 and orf19.9499 has a role in filamentation by both surface and embedded colonies, presumably as a sensor of environmental cues.     

Emission of low-frequency electromagnetic waves by a short laser pulse propagating in a plasma with density fluctuations

It is shown that a short laser pulse propagating in a plasma with electron density fluctuations can emit electromagnetic waves with frequencies much lower than the laser carrier frequency. Emissions with frequencies close to the plasma frequency and the doubled plasma frequency in a nonisothermal plasma, as well as emission generated in a turbulent plasma, are examined. The effects in question are related to the transformation of the laser pulse wakefield into electromagnetic radiation by electron density fluctuations. The phenomenon under study opens new possibilities for diagnostics of both plasma fields excited by laser pulses and electron density fluctuations in a plasma.     

On the theory of Cherenkov emission from a short laser pulse in a magnetized plasma

Cherenkov emission from a short laser pulse propagating in an underdense plasma along a constant magnetic field is considered. The spectral, angular, energy, and spatiotemporal parameters of the emitted radiation are investigated. It is shown that the spectral content of the radiation and its directionality depend sensitively on the plasma and laser-pulse parameters. For instance, the most intense backward radiation at the upper hybrid frequency is generated by a tightly focused laser pulse.     

Dipole Mechanism Generation of Terahertz Waves under Laser-Cluster Interaction

The feasibility of dipole radiation of terahertz waves under the action of a femtosecond laser pulse on a cluster is demonstrated theoretically. It is shown that the dipole mechanism of terahertz radiation generation plays a decisive role in the interaction of a laser pulse with small-size clusters with a sufficiently high electron collision frequency. The dependences of the spectral, angular, energetic, and spatiotemporal characteristics of the terahertz signal on the density of free electrons in the cluster plasma under the conditions in which dipole radiation is dominant are investigated. It is shown that the energy of terahertz radiation is maximal under the resonance conditions, when the laser frequency coincides with the eigenfrequency of a spherical cluster.     

Laser microirradiation of cerebellar neurons in culture

Electrophysiological and ultrastructural effects of focused laser radiation on neurons from neonatal rat cerebellum in tissue culture are reported. Action potentials were elicited by an extracellular current pulse train. The stimulator voltage required for half-maximum response frequency was measured as a function of the energy delivered by a single laser pulse. Above a “threshold” laser energy, the cell response to stimulation became negligible for all stimulator voltages. Electron micrographs of cells revealed that the mitochondria are preferentially damaged at an energy comparable to the electrophysiological threshold. The damaged mitochondria showed swollen matrix space and disrupted cristae membranes. Higher laser energies resulted in damage to other cytoplasmic structures. The results are consistent with a model that assumes that light interaction with the nerve cells proceeds by local heating of the mitochondria and nearby structures and leads to an increased conductance of the membrane to some ionic species.