共查询到20条相似文献,搜索用时 31 毫秒
1.
L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
2.
R. N. Morgan J. P. Wilson W. W. Hanna P. Ozias-Akins 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):413-420
Pearl millet [Pennisetum glaucum (L.) R.Br.] is a warm-season grass used for food, feed, fodder and forage, primarily in countries of Africa and India but
grown around the world. The two most-destructive diseases to pearl millet in the United States are rust (caused by Puccinia substriata var. indica) and pyricularia leaf spot (caused by Pyricularia grisea). Genes for disease resistance to both pathogens have been transferred into agronomically acceptable forage and grain cultivars.
A study was undertaken to identify molecular markers for three rust loci and one pyricularia resistance locus. Three segregating
populations were screened for RAPDs using random decamer primers and for RFLPs using a core set of probes detecting single-copy
markers on the pearl millet map. The rust resistance gene Rr
1
from the pearl millet subspecies P. glaucum ssp. monodii was linked 8.5 cM from the RAPD OP-G8350. The linkage of two RFLP markers, Xpsm108 (15.5 cM) and Xpsm174 (17.7 cM), placed the Rr
1
gene on linkage-group 3 of the pearl millet map. Rust resistance genes from both Tift 89D2 and ICMP 83506 were placed on linkage-group 4 by determining genetic linkage to the RFLP marker Xpsm716 (4.9 and 0.0 cM, respectively). Resistance in ICMP 83506 was also linked to the RFLP marker Xpsm306 (10.0 cM), while resistance in Tift 89D2 was linked to RAPD markers OP-K19350 (8.8 cM) and OP-O8350 (19.6 cM). Fragments from OP-K19 and OP-O8 in the ICMP 83506 population, and Xpsm306 in the Tift 89D2 population, were monomorphic. Only one RAPD marker (OP-D11700, 5.6 cM) was linked to pyricularia leaf spot resistance. Attempts to detect polymorphisms with rice RFLP probes linked to
rice blast resistance (Pyricularia oryzae; syn=P. grisea) were unsuccessful.
Received: 19 May 1997 / Accepted: 21 October 1997 相似文献
3.
J. P. W. Haanstra C. Wye H. Verbakel F. Meijer-Dekens P. van den Berg P. Odinot A. W. van Heusden S. Tanksley P. Lindhout J. Peleman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):254-271
Two independent F2 populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated
AFLP map. This map spanned 1482 cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199 cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23 cM; on the distal parts 1283 cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6 cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199 cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283 cM on the more distal parts, corresponding to one marker per 12 and 16 cM
respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci
showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for
genetic and breeding purposes and map-based cloning.
Received: 3 September 1998 / Accepted: 27 October 1998 相似文献
4.
A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms 总被引:1,自引:0,他引:1
S. E. Travis K. Ritland T. G. Whitham P. Keim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):871-880
Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer
combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed
significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with
another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers,
averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked
markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome.
A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked
72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents
an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in
pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
Received: 14 October 1997 / Accepted: 29 April 1998 相似文献
5.
Construction of an RFLP linkage map for cultivated sunflower 总被引:5,自引:0,他引:5
C. C. Jan B. A. Vick J. F. Miller A. L. Kahler E. T. Butler III. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):15-22
An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus
a fertility restoration gene, Rf
1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation,
with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters
of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with
11 markerless regions exceeding 20 cM.
Received: 17 June 1997 / Accepted: 19 June 1997 相似文献
6.
B. S. Vivek P. W. Simon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):58-64
A 109-point linkage map consisting of three phenotypic loci (P
1, Y
2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment
length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for
carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence
of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and θ=0.25 indicated 11 linkage groups. The
total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from
P
1, AFLP P1B34 was 2.2 cM from Y
2, and AFLP P3B30XA was 8.1 cM from Rs.
Received: 2 September 1998 / Accepted: 28 November 1998 相似文献
7.
A genetic map of melon (Cucumis melo L.) based on amplified fragment length polymorphism (AFLP) markers 总被引:3,自引:0,他引:3
Y.-H. Wang C. E. Thomas R. A. Dean 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):791-798
Genetic maps facilitate the study of genome structure and evolution, and the identification of monogenic traits or Mendelian
components of quantitative traits. We evaluated 228 RAPD, microsatellite and AFLP markers for linkage analysis in melon (Cucumis melo L.) varieties MR-1 (resistant to Fusarium wilt, powdery and downy mildews) and Ananas Yokneum (AY; susceptible to these diseases) and constructed a detailed genetic
map. The mapping population consisted of 66 backcross progenies derived from AY×(MR-1×AY). Despite a relatively low level
of polymorphism in the species, AFLP markers were found to be more efficient in mapping the melon genome than RAPD or microsatellite
markers. The map contains 197 AFLPs, six RAPDs and one microsatellite marker assigned to 14 major and six minor linkage groups,
and covers 1942 cM with the average distance between adjacent markers of approximately 10 cM. The maximum distance allowed
between markers is 27.5 cM. About 11% of the intervals (20 out of 173) are over 20 cM (but less than 27.5 cM). The map has
immediate utility for identifying markers linked to disease resistance genes that are suitable for marker-assisted breeding.
The use of microsatellite markers for integration with other maps is also discussed.
Received: 12 March 1997 / Accepted: 20 May 1997 相似文献
8.
RAPD and SCAR markers linked to the Ma1 root-knot nematode resistance gene in Myrobalan plum (Prunus cerasifera Ehr.) 总被引:1,自引:0,他引:1
A. C. Lecouls M. J. Rubio-Cabetas J. C. Minot R. Voisin A. Bonnet G. Salesses E. Dirlewanger D. Esmenjaud 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):328-335
The Myrobalan plum (Prunus cerasifera) is a self-incompatible species in which the clones P.2175, P.1079 and P.2980 are highly resistant to all root-knot nematodes
(RKN), Meloidogyne spp. Each clone bears a single major dominant gene, designated Ma1, Ma2 and Ma3 respectively, that controls a high and wide-spectrum resistance. Bulked segregant analysis (BSA) and random amplified polymorphic
DNA (RAPD) analysis were both performed to detect markers linked to the Ma1 gene using three segregating progenies from P.2175 (Ma1 ma1) crossed by three host parents (ma1 ma1). Four dominant coupling-phase markers were identified from a total of 660 10-base primers tested. The resulting linkage
map spans 14.7 cM and comprises three markers located on the same side of Ma1 and one marker located on the other side. The nearest markers (OPAL19720 and OPA161400) are located at 3.7 and 6.7 cM, respectively, on each side of the gene. Among the three markers that could be successfully
converted into sequence characterized amplified region (SCAR) markers, two of them (SCAL19690 and SCAN12620) were scored as dominant markers whereas the third (SCAO19770) failed to produce any polymorphism. SCAL19, and to a lesser extent SCAN12, can be used reliably in the marker-assisted selection
of Prunus rootstocks. These markers are adequate to identify the Ma1 RKN resistance gene in intraspecific segregating progenies and will be suitable for the creation of interspecific rootstocks
involving Myrobalan plum. Some of the RAPD and SCAR markers for Ma1 were also recovered in clones P.1079 and P.2980, but not in additional host clones, suggesting that Ma1, Ma2 and Ma3 are either allelic or at least closely linked.
Received: 22 September 1998 / Accepted: 19 December 1998 相似文献
9.
J. H. Peng T. Fahima M. S. Röder Y. C. Li A. Dahan A. Grama Y. I. Ronin A. B. Korol E. Nevo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):862-872
Stripe rust caused by Puccinia striifomis West. is one of the most devastating diseases relating to wheat production. Wild emmer wheat, Triticum dicoccoides, the tetraploid progenitor of cultivated wheat, has proven to be a valuable source of novel stripe-rust resistance genes
for wheat breeding. For example, T. dicoccoides accessions from Mt. Hermon, Israel, are uniformly and highly resistant to stripe-rust. The main objective of the present
study is to map a stripe-rust resistance gene, derived from the unique Mt. Hermon population of wild emmer, using microsatellite
markers. An F2 mapping population was established by crossing stripe-rust resistant T. dicoccoides accession H52 from Mt. Hermon with the Triticum durum cultivar Langdon. The stripe-rust resistance derived from accession H52 was found to be controlled by a single dominant gene
which was temporarily designated as YrH52. Out of 120 microsatellite markers tested, 109 (91%) showed polymorphism between the parental lines. Among 79 segregating
microsatellite loci generated from 56 microsatellite primer pairs, nine were linked to YrH52 with recombination frequencies of 0.02–0.35, and LOD scores of 3.56–54.22. A genetic map of chromosome 1B, consisting of
ten microsatellite loci and the stripe-rust resistance gene YrH52, was constructed with a total map length of 101.5 cM. YrH52 is also closely linked to RFLP marker Nor1 with a map distance of 1.4 cM and a LOD value of 29.62. Apparent negative crossover interference was observed in chromosome
1B, especially in the region spanning the centromere. Negative crossover interference may be a common characteristic of gene-rich
regions or gene clusters in specific chromosomes.
Received: 30 October 1998 / Accepted: 2 November 1998 相似文献
10.
Locating the petunia Rf gene on a 650-kb DNA fragment 总被引:1,自引:0,他引:1
S. Bentolila J. Zethof T. Gerats M. R. Hanson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):980-988
A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf ) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf (<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement between the petunia and the tomato genomes, this
synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf. Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb MluI fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.
Received: 20 August 1997 / Accepted: 21 October 1997 相似文献
11.
S. C. K. Milach H. W. Rines R. L. Phillips 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):783-790
Restriction fragment length polymorphism (RFLP) analysis provides a valuable tool for characterizing and understanding relationships
among genes for useful traits in crop species, particularly in ones with complex genomes such as the hexaploid cultivated
oat Avena sativa L. (2n=6x=42). Using Bulked Segregant Analysis (BSA) and F2 RFLP linkage data, we mapped three dominant oat dwarfing loci to different regions of the oat genome. Dw6, in oat line OT207, is 3.3±1.3 cM from the Xumn145B locus, which has not been placed on the hexaploid oat linkage map. Dw7, in line NC2469-3, is 4.3±2.3 cM from Xcdo1437B and 33±4.1 cM from Xcdo708B. This places Dw7 to linkage group 22. Dw8, in the Japanese lines AV17/3/10 and AV18/2/4, mapped 4.9±2.2 cM from Xcdo1319A in an AV17/3/10בKanota’ F2 population and 6.6±2.6 cM from it in an AV18/2/4בKanota’ population. This places Dw8 to linkage group 3. Aneuploid analysis of markers linked to the dwarfing genes located Dw6 on the smallest oat chromosome (chromosome 18) and Dw7 on the longest satellited chromosome (chromosome 19). The RFLP markers closely linked to the three dwarfing genes identify
distinct regions of the oat genome that contribute to plant height and they should be useful in characterizing new genetic
sources of dwarfness in oat.
Received: 8 May 1997 / Accepted: 20 May 1997 相似文献
12.
S. Fondevilla D. Rubiales M. T. Moreno A. M. Torres 《Molecular breeding : new strategies in plant improvement》2008,22(2):193-200
Three genes, er1, er2 and Er3, conferring resistance to powdery mildew (Erysiphe pisi) in pea have been described so far. Because single gene-controlled resistance tends to be overcome by evolution of pathogen
virulence, accumulation of several resistance genes into a single cultivar should enhance the durability of the resistance.
Molecular markers linked to genes controlling resistance to E. pisi may facilitate gene pyramiding in pea breeding programs. Molecular markers linked to er1 and er2 are available. In the present study, molecular markers linked to Er3 have been obtained. A segregating F2 population derived from the cross between a breeding line carrying the Er3 gene, and the susceptible cultivar ‘Messire’ was developed and genotyped. Bulk Segregant Analysis (BSA) was used to identify
Random Amplified Polymorphic DNA (RAPD) markers linked to Er3. Four RAPD markers linked in coupling phase (OPW04_637, OPC04_640, OPF14_1103, and OPAH06_539) and two in repulsion phase
(OPAB01_874 and OPAG05_1240), were identified. Two of these, flanking Er3, were converted to Sequence Characterized Amplified Region (SCAR) markers. The SCAR marker SCW4637 co-segregated with the resistant gene, allowing the detection of all the resistant individuals. The SCAR marker SCAB1874, in repulsion phase with Er3, was located at 2.8 cM from the gene and, in combination with SCW4637, was capable to distinguish homozygous resistant individuals from heterozygous with a high efficiency. In addition, the validation
for polymorphism in different genetic backgrounds and advanced breeding material confirmed the utility of both markers in
marker-assisted selection. 相似文献
13.
Rai R Singh AK Singh BD Joshi AK Chand R Srivastava CP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(5):803-813
Pea rust caused by Uromyces fabae (Pers.) de-Bary is a major problem in warm humid regions causing huge economic losses. A mapping population of 136 F6:7 recombinant inbred lines (RILs) derived from the cross between pea genotypes, HUVP 1 (susceptible) and FC 1 (resistant) was
evaluated in polyhouse as well as under field conditions during two consecutive years. Infection frequency (IF) and area under
disease progress curve (AUDPC) were used for evaluation of rust reaction of the RILs. A linkage map was constructed with 57
polymorphic loci selected from 148 simple sequence repeats (SSRs), 3 sequence tagged sites (STS), and 2 random amplified polymorphic
(RAPD) markers covering 634 cM of genetic distance on the seven linkage groups of pea with an average interval length of 11.3 cM.
Composite interval mapping (CIM) revealed one major (Qruf) and one minor (Qruf1) QTL for rust resistance on LGVII. The LOD (5.2–15.8) peak for Qruf was flanked by SSR markers, AA505 and AA446 (10.8 cM), explaining 22.2–42.4% and 23.5–58.8% of the total phenotypic variation
for IF and AUDPC, respectively. The minor QTL was environment-specific, and it was detected only in the polyhouse (LOD values
4.2 and 4.8). It was flanked by SSR markers, AD146 and AA416 (7.3 cM), and explained 11.2–12.4% of the total phenotypic variation.
The major QTL Qruf was consistently identified across all the four environments. Therefore, the SSR markers flanking Qruf would be useful for marker-assisted selection for pea rust (U. fabae) resistance. 相似文献
14.
N. Nitta M. L. Farman S. A. Leong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(1-2):20-32
A high-density genetic map of the rice blast fungus Magnaporthe grisea (Guy11×2539) was constructed by adding 87 cosmid-derived RFLP markers to previously generated maps. The new map consists
of 203 markers representing 132 independently segregating loci and spans approximately 900 cM with an average resolution of
4.5 cM. Mapping of 33 cosmid probes from the genetic map generated by Sweigard et al. has allowed the integration of two M. grisea maps. The integrated map showed that the linear order of markers along all seven chromosomes in both maps is in good agreement.
Thirty of eighty seven markers were derived from cosmid clones that contained the retrotransposon MAGGY (M. grisea gypsy element). Mapping of single-copy DNA sequences associated with the MAGGY cosmids indicated that MAGGY elements are
scattered throughout the fungal genome. In eight cases, the probes associated with MAGGY elements showed abnormal segregation
patterns. This suggests that MAGGY may be involved in genomic rearrangements. Two RFLP probes linked to MAGGY elements, and
another flanking other repetitive DNA elements, identified sequences that were duplicated in the Guy11 genome. Most of the
MAGGY cosmids also contained other classes of repetitive DNA suggesting that repetitive DNA sequences tend to cluster in the
M. grisea genome.
Received: 17 February 1997 / Accepted: 21 February 1997 相似文献
15.
C. S. Echt C. D. Nelson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(8):1031-1037
Haploid linkage analysis of eastern white pine, Pinus strobus L., was carried out using mainly RAPD markers and microsatellite, or simple-sequence-repeat, markers. Ninety one loci mapped
to 12 linkage groups of three or more markers. The resulting framework genome map, the first for a soft pine species, contained
69 markers. The map covered 58% of the estimated genome length of 2071 cM(K), with a 95% confidence interval of 1828–2242 cM(K).
A systematic comparison of linkage data from eastern white pine, longleaf pine (P. palustris Mill.) and maritime pine (P. pinaster Ait.), gave genome-length estimates for all three species very close to either 2000 cM(K) or 2600 cM(H), depending on whether
the Kosambi(K) or Haldane(H) map functions, respectively, were employed. Differences among previous pine genome-length estimates
were attributed to the divergent criteria used in the methods of estimation, and indicate the need for the adoption of uniform
criteria when performing genome-length estimates. Current data suggest that members of the two pine subgenera, which diverged
during the late Mesozoic era, have highly conserved rates of recombination.
Received: 5 January 1997/Accepted: 24 January 1997 相似文献
16.
K. J. Williams A. Lichon P. Gianquitto J. M. Kretschmer A. Karakousis S. Manning P. Langridge H. Wallwork 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):323-327
Spot form of net blotch (SFNB) (Pyrenophora teres f maculata) is an economically damaging foliar disease of barley in many of the world’s cereal growing areas. The development of SFNB-resistant
cultivars may be accelerated through the use of molecular markers. A screen for SFNB resistance in 96 lines identified four
new sources of resistance, including a feed variety, ‘Galleon’, for which a fully mapped doubled haploid population was available.
Segregation data indicated SFNB resistance was conferred by a single gene in the ‘Galleon’בHaruna Nijo’ cross, positioned
on the long arm of chromosome 7H. This gene is designated Rpt4 and is flanked by the RFLP loci Xpsr117(D) and Xcdo673 at distances of 6.9 cM and 25.9 cM, respectively. The marker Xpsr117(D) was validated using another population segregating for Rpt4, correctly predicting SFNB resistance with more than 90% accuracy.
Received: 24 September 1998 / Accepted: 19 December 1998 相似文献
17.
M. C. Moretzsohn C. D. M. Nunes M. E. Ferreira D. Grattapaglia 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):63-70
Shell thickness is an important trait in oil palm breeding programs and is the basis for the classification of the varieties
of oil palm into the types dura, tenera and pisifera. This trait seems to be controlled by a single locus, with two alleles
(sh
+ and sh
−) showing codominant expression. Two single-tree linkage maps were constructed for a maternal tenera (sh
+
sh
−) palm and for a paternal pisifera (sh
−
sh
−) palm using the pseudo-testcross mapping strategy in combination with RAPD markers through the analysis of an F1 tenera×pisifera progeny. A total of 308 arbitrary primers were screened in a sample of eight F1 plants and 121 markers were detected in a testcross configuration. An average of 1.66 polymorphic marker per selected primer
were identified in this cross. At LOD 5.0 (with some few exceptions) and θ=0.25 the maternal tenera map included a total of
48 markers distributed in 12 linkage groups or pairs of markers (449.3 cM) while the paternal pisifera map included 42 markers
distributed in 15 linkage groups or pairs of markers (399.7 cM). We used RAPD and bulked segregant analysis (BSA) to identify
markers more tightly linked to the sh
+ locus. A total of 174 new primers not previously used in the linkage analysis were screened using bulks of DNA extracted
from plants selected for the contrasting shell-thickness phenotypes. Two RAPD markers (R11–1282 and T19–1046) were identified
to be linked on both sides of the sh
+ locus on linkage group 4. The estimated map distances from sh
+ to R11–1282 and to T19–1046 were 17.5 cM and 23.9 cM, respectively. The results demonstrate the usefulness of RAPD markers
and the pseudo-testcross mapping strategy for developing genetic linkage information, and constitute an important step towards
early marker-assisted selection for shell thickness in oil palm.
Received: 21 February 1999 / Accepted: 29 April 1999 相似文献
18.
C. Rameau D. Dénoue F. Fraval K. Haurogné J. Josserand V. Laucou S. Batge I. C. Murfet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):916-928
Random amplified polymorphic DNA (RAPD) markers linked to two morphological markers ( fa and det), three ramosus genes (rms2, rms3 and rms4) and two genes conferring flowering response to photoperiod in pea (sn, dne) were selected by bulk segregant analysis on F2 populations. Two RAPD fragments were cloned and sequenced to generate the two SCAR markers V20 and S2 which are linked to
rms3 and dne, respectively. All these genes, except rms2, were previously located on the pea classical linkage map. Rms2 mapped to linkage group IB which contains the afila gene. Precise genetic maps of the regions containing the genes were obtained and compared to the RAPD map generated from
the recombinant inbred-lines population of the cross Térèse×K586. This cross was chosen because several mutants were obtained
from cultivars Térèse and Torsdag (K586 was derived from Torsdag). This collection of isogenic lines was used for the construction
of F2 mapping populations in which polymorphic RAPD markers were already known and mapped. Moreover, the well-known problem in
pea of variability in the linkage associations between crosses was avoided. This work contributes to the precise integration
between the classical map and the molecular maps existing in pea.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
19.
J. M. Kretschmer K. J. Chalmers S. Manning A. Karakousis A. R. Barr A. K. M. R. Islam S. J. Logue Y. W. Choe S. J. Barker R. C. M. Lance P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(8):1060-1064
The cereal cyst nematode (CCN), Heterodera avenae Woll., is an economically damaging pest of barley in many of the world’s cereal-growing areas. The development of CCN-resistant
cultivars may be accelerated through the use of molecular markers. A number of resistance genes against the pest are well
known; one of them, the single dominant Ha 2 resistance gene, has been shown to be effective against the Australian pathotype and maps to chromosome 2 of barley. Segregation
analysis identified two restriction fragment length polymorphism (RFLP) markers flanking the resistance gene in two doubled-haploid
populations of barley. AWBMA 21 and MWG 694 mapped 4.1 and 6.1 cM respectively from the Ha 2 locus in the Chebec×Harrington cross and 4.0 and 9.2 cM respectively in the Clipper×Sahara cross. Analysis of a further seven
sources of CCN resistance in the form of near-isogenic lines (NILs) indicates that all available sources of resistance to
the Australian pathotype of CCN in barley represent the Ha 2 locus.
Received: 5 December 1996 / Accepted: 20 December 1996 相似文献
20.
Genetics of resistance to ascochyta blight (Ascochyta lentis) of lentil and the identification of closely linked RAPD markers 总被引:1,自引:0,他引:1
R. Ford E. C. K. Pang P. W. J. Taylor 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):93-98
Foliar resistance to Ascochyta lentis is controlled at a single major locus by a dominant gene (AbR
1
) in the lentil accession ILL5588 (cv ‘Northfield’). Flanking RAPD markers that are closely linked to the resistance locus
in coupling phase were identified by bulked segregant analysis. Out of 261 decanucleotide primers screened 7 produced a polymorphic
marker that segregated with the resistance locus, and all markers were found to exist within a single linkage group. Five
of the seven RAPD markers were within 30 cM of the resistance locus. Log likelihood analysis for detecting QTL associated
with the foliar resistance revealed that a single narrow peak accounted for almost 90% of the variance of resistance between
the bulks. Preliminary mapping in an F3 population revealed that the closest flanking markers were approximately 6 and 14 centiMorgans (cM) away from the resistance
locus. These markers should be useful for the discrimination of resistant germplasm through marker-assisted selection in future
breeding programmes and represent the first essential step towards the map-based cloning of this resistance gene.
Received: 18 December 1997 / Accepted: 9 June 1998 相似文献