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1.
镇海棘螈的精子结构   总被引:2,自引:0,他引:2  
郑中华  谢锋  江建平  费梁 《动物学报》2004,50(4):622-629
应用透射电镜和扫描电镜对镇海棘螈Echinotritonchinhaiensis精子的超微结构进行了研究。结果表明 :镇海棘螈的精子具有蝾螈科精子的一些共同特征 ,如精子头部较长、前端有顶体钩、细胞核周围有核脊、尾部轴丝旁有边缘丝 ,但也具有其自身的形态结构特点 ,如精子颈部的长度、核脊的形状和大小等与有尾两栖类物种的精子存在明显差别。镇海棘螈精子发达的颈部和丰富的核脊具类群特异性  相似文献   

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镇海棘螈及濒危现状   总被引:7,自引:1,他引:7       下载免费PDF全文
费梁 《动物学杂志》1992,27(4):39-41
本文简述了镇海棘螈的研究概况、外部形态、生活习性及其研究价值。调查资料表明,棘螈分布区极窄,数量甚少,已处于极度濒危。同时对濒危原因作了分析,提出了保护措施和建议。  相似文献   

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镇海棘螈产卵位点及其对繁殖成功率的影响   总被引:5,自引:0,他引:5  
We studied oviposition sites and their effects on the reproductive success of the Chinhai salamander (Echinotriton chinhaiensis) .The results showed that: (1) the female had strong preferences for spawning sites and oviposition microhabitat; (2) the most suitable oviposition sites were 30 cm higher than water level and 45 cm from the water body; (3) oviposition sites usually overlapped;(4) embryos could develop and hatch normally in water as well as on land. The hatching rate on land was high (94 3%), but the number of hatchlings that successfully migrated to water was low (52%±11%, n =3 in the laboratory, and approximatly 20% in nature). Based on the situation in laboratory, the average hatchling survival rate at all oviposition sites was 36 7%. Owing to oviposition on land, the reproductive success of Chinhai salamander was very low;(5) protecting the spawning sites and increasing the hatchling survival rate are the keys to conserving this species.  相似文献   

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将镇海棘螈Echinotriton chinhaiensis瑞岩寺种群2008年的繁殖情况与1998、1999、2000年的繁殖量进行比较,发现虽然雌性平均窝卵数比较稳定,但2008年的繁殖总量小于其余3年任何一年总产卵量的50%.对其繁殖量大幅下降的原因进行了分析,发现2007年9、10月两次影响严重的强台风、瑞岩寺景区开发等因素可能是造成种群繁殖量大幅下降的原因,而2008年初50年不遇的低温是否影响镇海棘螈的繁殖值得进一步追踪研究.  相似文献   

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研究小组在教师指导下,积极探索标记镇海棘螈的方法,形成了若干方案,并对每个方案的操作方法和优缺点进行论述。  相似文献   

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镇海棘螈极度濒危.柴桥中学研究小组在教师的指导下对镇海棘螈的濒危原因进行考察、分析和归类,并提出保护建议。  相似文献   

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文县疣螈早期胚胎发育   总被引:1,自引:0,他引:1       下载免费PDF全文
对湖南省怀化市黄岩地区的文县疣螈(Tylototriton wenxianensis)早期胚胎发育进行了观察。整个胚胎发育分为21个时期,在实验室19~21℃下,受精卵完成胚胎发育历时(513.30±7.93)h(n=8);本文描述了各时期的形态特征,并讨论了与其他有尾类胚胎发育的异同,以及怀化产文县疣螈与甘肃产文县疣螈的主要区别。  相似文献   

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我国棘螈属(Echinotriton)和疣螈属(Tylototriton)的研究概况   总被引:2,自引:1,他引:2  
余平静  赵尔宓 《四川动物》2005,24(4):646-650
棘螈属(Echinotriton)和疣螈属(Tylototriton)均属于两栖纲有尾目蝾螈科.两属主要分布于我国西南、亚洲西南部、东南亚及日本.棘螈属在我国已知3种:Echinotriton andersoni(琉球棘螈)、E. Chinhaiensis(镇海棘螈)、E. Asperrimus(细痣棘螈).疣螈属在我国已知4种:Tylototriton kweichowensis(贵州疣螈)、T. Shanjing(红瘰疣螈)、T.taliangensis(大凉疣螈)、T. Verrucosus(棕黑疣螈).本文回顾了棘螈属和疣螈属物种的命名史,阐明了两属之间的关系,并从形态学、生态学、细胞遗传学和分子生物学方面对这两属的研究工作进行了综述.  相似文献   

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红瘰疣螈胚胎发育的初步观察   总被引:1,自引:0,他引:1  
报道红瘰疣螈(Tylototmon shanjing)的胚胎发育过程。根据胚胎发育过程中的形态变化规律将胚胎发育过程分为22个时期,在室温(25±1)℃的条件下,胚胎发育历时20-22d。描述了各时期的形态特征,并讨论了发育中的一些现象。  相似文献   

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Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. ehinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.  相似文献   

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Fos-like antigens (Fosl) including Fosl1 and Fosl2 exclusively heterodimerize with Jun members to form AP-1 complex, thereby participating in various cellular progresses including cell cycle regulation. However, expression patterns of these two genes during embryonic development remains largely unknown. In the present study, both temporal and spatial expression patterns of fosl1 and fosl2 were examined during embryonic development of Xenopus tropicalis. Real-time quantitative PCR results showed that the expression of the two genes was increased from stage 2 to stage 42. However, expression level of fosl1 is much higher than that of fosl2 at stage 42. Whole-mount in situ hybridization showed that fosl1 was expressed in eyes, branchial arch, notochord, otic vesicle, and liver. However, fosl2 was expressed in lung primordium from stage 34 to stage 38, in addition to the moderate expression in eyes and branchial arch at stage 42. Thus, the developmental expression patterns of these two fosl genes is different in Xenopus embryos. These results provide a basis for further functional study of these two genes.  相似文献   

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Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19–39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.  相似文献   

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脂肪酶以其独特的优势在各个领域都有其潜在的应用前景。在高等植物中,种子萌发过程中脂肪酶活性较高,其活性与食品的加工方法有关。应用不同的方法测定大麦脂肪酶酶活力,得到的结果也不同。详细介绍了大麦脂肪酶的部分酶学性质。  相似文献   

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