EARLY DEVONIAN FISHES FROM GUIJIATUN AND XUJIACHONG FORMATIONS OF QUJING,YUNNAN,AND RELATED BIOSTRATIGRAPHIC PROBLEMS

Pale0nt0IogicStudiesSubclassGaleaspidaHalsteadTarl0,l9670rderHuanana8piformesJanvier,l975FamilyGantar0stra8pidaeWangetWang,l992GenusCantarostraspisWangetWang,l992CQntarostra8pisgeniWangetWang,l992(Fig.l3Pl-II,4-5)1992,Gantarostraspisgeni,Wang,J.Q.etWang,N.Z.,Fig-l,Pl.l.HolotypeAdorsalshieldV9758,LowerDevonianPosongchongF0rmation,Gu-mu,Wenshandistrict,Yunnan-ReferredspeeimensAdorsalshieldVlO494.1;arostra1pr0cessV1O494.2.Loca1ityandh0riz0nXujiach0ng,Xishan,Qujing,Yunnan,Lowe…     

DNA Polymerase β Ribonucleotide Discrimination: INSERTION,MISINSERTION, EXTENSION,AND CODING*

DNA polymerases must select nucleotides that preserve Watson-Crick base pairing rules and choose substrates with the correct (deoxyribose) sugar. Sugar discrimination represents a great challenge because ribonucleotide triphosphates are present at much higher cellular concentrations than their deoxy-counterparts. Although DNA polymerases discriminate against ribonucleotides, many therapeutic nucleotide analogs that target polymerases have sugar modifications, and their efficacy depends on their ability to be incorporated into DNA. Here, we investigate the ability of DNA polymerase β to utilize nucleotides with modified sugars. DNA polymerase β readily inserts dideoxynucleoside triphosphates but inserts ribonucleotides nearly 4 orders of magnitude less efficiently than natural deoxynucleotides. The efficiency of ribonucleotide insertion is similar to that reported for other DNA polymerases. The poor polymerase-dependent insertion represents a key step in discriminating against ribonucleotides because, once inserted, a ribonucleotide is easily extended. Likewise, a templating ribonucleotide has little effect on insertion efficiency or fidelity. In contrast to insertion and extension of a ribonucleotide, the chemotherapeutic drug arabinofuranosylcytosine triphosphate is efficiently inserted but poorly extended. These results suggest that the sugar pucker at the primer terminus plays a crucial role in DNA synthesis; a 3′-endo sugar pucker facilitates nucleotide insertion, whereas a 2′-endo conformation inhibits insertion.     

DIURNAL,AGE, AND IMMUNE REGULATION OF INTERLEUKIN-1β AND INTERLEUKIN-1 TYPE 1 RECEPTOR IN THE MOUSE SUPRACHIASMATIC NUCLEUS

Circadian clocks serve to impose a near-24-h temporal architecture on an organism's physiology, metabolism, and behavior. In mammals, the suprachiasmatic nucleus (SCN) of the hypothalamus functions as the master circadian pacemaker. There is growing evidence that immunomodulators, such as cytokines, may impinge on circadian timekeeping. We examined whether there is endogenous expression of the proinflammatory cytokine interleukin-1β (IL-1β) and its signaling receptor IL-1R1 in the SCN of young and older mice across the diurnal cycle. We found expression of both IL-1β and IL-1R1 in the young SCN, although only IL-1R1 displayed temporal regulation. In the older SCN, levels of IL-1β were expressed at lower levels than in the young SCN, and IL-1R1 did not vary across the 24?h. We also report age-related day-night variation of IL-1β and IL-1R1 in the paraventricular nucleus (PVN) of the hypothalamus. Further, we examined the effect of peripheral immune challenge on IL-1β and IL-1R1 in the SCN. We found that IL-1β immunoreactivity was not altered 6 or 24?h after a septic dose of lipopolysaccharide (LPS; 5?mg/kg), whereas IL-1R1 was significantly up-regulated in the SCN both 6 and 24?h after LPS. We also demonstrate cellular activation in the SCN 24?h following LPS treatment, as evidenced by increased c-Fos and p65-NF-κB (nuclear factor kappa B) expression. Our results indicate that IL-1β and its associated signaling system may play a role in mediating the response of the circadian timing system to immune challenge as well as potentially contributing to the basal functioning of the SCN clock. (Author correspondence: andrew.coogan@nuim.ie)     

CARBONIFEROUS-PERMIAN BOUNDARY BEDS AND FUSULINID ZONES AT XIAODUSHAN, GUANGNAN, EASTERN YUNNAN

The Xiaodushan section is situated at about 5 km from the Babao village in Guangnan County in eastern Yunnan. In this section, the stratigraphic suecession from Lower Carboniferous to Middle Permian is continuous and ex-     

IS LIFE IMPOSSIBLE? INFORMATION,SEX, AND THE ORIGIN OF COMPLEX ORGANISMS

The earliest organisms are thought to have had high mutation rates. It has been asserted that these high mutation rates would have severely limited the information content of early genomes. This has led to a well‐known “paradox” because, in contemporary organisms, the mechanisms that suppress mutations are quite complex and a substantial amount of information is required to construct these mechanisms. The paradox arises because it is not clear how efficient error‐suppressing mechanisms could have evolved, and thus allowed the evolution of complex organisms, at a time when mutation rates were too high to permit the maintenance of very substantial amounts of information within genomes. Here, we use concepts from the formal theory of information to calculate the amount of genomic information that can be maintained. We identify conditions under which much higher levels of genomic information can be maintained than previously considered possible among origin‐of‐life researchers. In particular, we find that the highest levels of information are maintained when many genotypes produce identical phenotypes, and when reproduction occasionally involves recombination between multiple parental genomes. There is a good reason to believe that these conditions are relevant for very early organisms, and thus the results presented may provide a solution to a long‐standing logical problem associated with the early evolution of life.     

WHY DOES BONE MATRIX CONTAIN NON-COLLAGENOUS PROTEINS? THE POSSIBLE ROLES OF OSTEOCALCIN,OSTEONECTIN, OSTEOPONTIN AND BONE SIALOPROTEIN IN BONE MINERALISATION AND RESORPTION

Four major non-collagenous bone proteins were localised by single and double immuno-histochemistry during de novo mineralisation and bone resorption. Both osteopontin and bone sialoprotein were localised ahead of the mineralisation front, suggesting that both proteins are necessary for the initiation of bone mineralisation. This supports previous suggestions that bone sialoprotein acts as a crystal nucleator. The role of osteopontin is less certain, but might be related to ensuring that only the right type of crystal is formed. Osteocalcin and osteonectin were not present in areas of first crystal formation, but were present in the fully mineralised matrix. Their role may be to control the size and speed of crystal formation. Osteopontin, bone sialoproteins and osteocalcin (but not osteonectin) were also present at reversal lines. Interpreting this localisation together with information from the literature, the following functions are suggested during resorption: Osteocalcin may act as a chemoattractant for osteoclasts, while both osteopontin and bone sialoprotein may facilitate the binding of osteoclasts via the arg-gly-asp motif.     

SYNTHESIS AND PROPERTIES OF NOVEL TRIPHOSPHATE ANALOGUES: RIBONUCLEOSIDE AND DEOXYRIBONUCLEOSIDE (α-P-BORANO, α-P-THIO)TRIPHOSPHATES

The first ribo- and deoxyribo-nucleoside (α-P-borano, α-P-thio)triphosphates have been synthesized. The chemical and biochemical properties of adenosine (α-P-borano, α-P-thio)triphosphate and thymidine (α-P-borano, α-P-thio) triphosphate have been investigated.     

BIODIVERSITY AND CONSERVATION OF FRESHWATER FISH IN HONG KONG, CHINA

Hong Kong Special Administrative Region,China,has an area of1095km2and is located at the edge of thenorthern tropical zone.The landscape is dominated byhills and ravines withflat landrestrictedtothe lowflood-plains and coastal regions.Inthis hilly domain,the smallrocky streams are typically fast flowing at their uplandsources,and graduallyreduceinspeed asthey wind alonghill cuttings to reach the lowlands.While streams arecommonlyfound,rivers are very limited in number.Thefewlong and meanderi…     

AIMS AND SCOPE

The Journal of Bionic Engineering publishes original research papers and reviews on all aspects of bionic science and engineering including fundamental understandings of animals and plants for bionic engineering, such as locomotion and behaviors of animals, structures, composites, morphology and physical properties of plants and natural materials, applications of such understandings in engineering, technology and designs.     

NEW GENERA AND NEW SPECIES OF ACRIDIDAE FROM YUNNAN-KWEICHOW PLATAU, CHINA

In the present paper,two genera and ten species of Acrididae are described as newfrom Yunnan,Kweichow and northern part of Szechwan. All type specimens are deposited in the Department of Biology,Shensi NormalUniversity. Gesonula mundata szemaoensis ssp.nov.(fig.1)     

STRUCTURAL,DYNAMIC, AND ENZYMATIC PROPERTIES OF MIXED α/β-OLIGONUCLEOTIDES CONTAINING POLARITY REVERSALS

We employ NMR structure determination, thermodynamics, and enzymatics to uncover the structural, thermodynamic and enzymatic properties of α/β-ODNs containing 3′-3′ and 5′-5′ linkages. RNase H studies show that α/β-gapmers that are designed to target erbB-2 efficiently elicit RNase H activity. NMR structures of DNA · DNA and DNA · RNA duplexes reveal that single α-anomeric residues fit well into either duplex, but alter the dynamic properties of the backbone and deoxyriboses as well as the topology of the minor groove in the DNA · RNA hybrid.     

PENGUINS,PETRELS, AND PARSIMONY: DOES CLADISTIC ANALYSIS OF BEHAVIOR REFLECT SEABIRD PHYLOGENY?

Whether or not behavior accurately reflects evolutionary relationships (phylogeny) has been hotly debated by ethologists and comparative psychologists. Previous studies attempting to resolve this question have generally lacked a quantitative, phylogenetic approach. In this study we used behavior and life-history (BLH) information (72 characters) to generate phylogenetic trees for 18 seabird species (albatrosses, petrels, and penguins). We compared these trees with trees obtained from isozyme electrophoretic analysis of blood proteins (15 loci and 98 electromorphs) and partial mitochondrial 12S ribosomal DNA sequences (381 base pairs). Cladistic analysis of the BLH data set generated three MP trees (tree length = 243, CI = 0.52, RI = 0.57) with significant cladistic structure. The BLH characters were classified into four types (foraging, agonistic, reproductive, and life history) and levels of homoplasy for each type were measured. No significant differences were found among these categories. The BLH trees were shown to be significantly more congruent with the electrophoretic and 12S sequence trees than expected by chance. This indicates that seabird BLH data contains phylogenetic signal. Areas of incongruence between BLH trees and a phylogeny generated by combining the data sets were predicted to result from ecological constraints that did not covary with phylogeny. These predictions were supported by the results of a concentrated changes test. This study found that this BLH data set was no more homoplasious than molecular data and that BLH trees were significantly congruent with molecular trees.     

DESCRIPTION ON NEW SPECIES AND ZOOGEOGRAPHICAL ANALYSIS OF THE LAND MOLLUSKS FROM XISHUANGBANNA AND NEIGHBORING AREA, YUNNAN PROVINCE (GASTROPODA: PROSOBRANCHIA: ARCHAEOGASTROPDA, MESOGASTROPODA)

1Georissamenglunensissp.nov.(Fig.l)Holotype.Hight2.omm,breadth1.4mm;lengthofoperculumo.85mm,breadthofoperculumo.7Omm.Menglum,MenglaCounty(2l'O9'N,lOl'O2'E),YunnanProvince,China,July,27,l993.Paratypes:47specimens,hight2.2O-l.7Omm,breadthl.2O-1.55mm;lengthofapertureO.65-o.85mm,breadthofapertureO.7o-O.8Omm;Menglaun,MenglaCounty,YunnanProvince,China.July27,l993;May23,1994.TypespecimensaredepositedintheInstituteofZoologytheChineseAcademyofSci-ences,Beijing.1Shellsmall,thin.Whols47,we…     

WADERS (CHARADRII) AND OTHER WATERBIRDS AT LANGEBAAN LAGOON,SOUTH AFRICA, 1975–1986

Summary

Underhill, L. G. 1987. Waders (Charadrii) and other waterbirds at Langebaan Lagoon, South Africa, 1975–1986. Osrrich 58: 145–155.

Langebaan Lagoon was surveyed for waterbirds at midsummer and midwinter between 1975 and 1986. The median number of birds counted in summer was 37 500, of which 34 500 were waders (93% of the waders being Palaearctic migrants). Curlew Sandpiper (59,2%), Grey Plover (10,5%), Sanderling (8,3%), Knot (8,1%) and Turnstone (5,7%) were the major components of the summer wader population. The median number of birds in winter was 10 500, of which 4500 were flamingos and 4000 waders. For Palaearctic waders, the median winter population was 11,5% of the median summer population, but varied between 2,5% and 30,1%. For species of wader which breed in the Taimyr Peninsula, Siberia, a three-year cycle in the numbers of birds overwintering was detected, with large numbers in 1977, 1980, 1983 and 1986. Birds feeding on invertebrates consumed 126,9 kJ m^?2 yr^?1, or 24% of the total production of invertebrates. Greater Flamingos have a major impact on energy cycling at Langebaan Lagoon, accounting for 73,3% of the winter energy consumption by the avifauna. Langebaan Lagoon is the most important wetland for waders in South Africa, accounting for about 10% of the coastal wader population of South Africa. At midsummer, about 0,5% of the total wader population of the East Atlantic Flyway is at Langebaan Lagoon, which ranks about 20th in importance for waders on the flyway.     

PERSPECTIVE: SEX,RECOMBINATION, AND THE EFFICACY OF SELECTION—WAS WEISMANN RIGHT?

The idea that sex functions to provide variation for natural selection to act upon was first advocated by August Weismann and it has dominated much discussion on the evolution of sex and recombination since then. The goal of this paper is to further extend this hypothesis and to assess its place in a larger body of theory on the evolution of sex and recombination. A simple generic model is developed to show how fitness variation and covariation interact with selection for recombination and illustrate some important implications of the hypothesis: (1) the advantage of sex and recombination can accrue both to reproductively isolated populations and to modifiers segregating within populations, but the former will be much larger than the latter; (2) forces of degradation that are correlated across loci within an individual can reduce or reverse selection for increased recombination; and (3) crossing-over (which can occur at different places in different meioses) will create more variability than having multiple chromosomes and so will have more influence on the efficacy of selection. Several long-term selection experiments support Weismann's hypothesis, including those showing a greater response to selection in populations with higher rates of recombination and higher rates of recombination evolving as a correlated response to selection for some other character. Weismann's hypothesis is also consistent with the sporadic distribution of obligate asexuality, which indicates that clones have a higher rate of extinction than sexuals. Weismann's hypothesis is then discussed in light of other patterns in the distribution of sexuality versus asexuality. To account for variation in the frequency of obligate asexuality in different taxa, a simple model is developed in which this frequency is a function of three parameters: the rate of clonal origin, the initial fitness of clones when they arise, and the rate at which that fitness declines over time. Variation in all three parameters is likely to be important in explaining the distribution of obligate asexuality. Facultative asexuality also exists, and for this to be stable it seems there must be ecological differences between the sexual and asexual propagules as well as genetic differences. Finally, the timing of sex in cyclical parthenogens is most likely set to minimize the opportunity costs of sex. None of these patterns contradict Weismann's hypothesis, but they do show that many additional principles unrelated to the function of sex are required to fully explain its distribution. Weismann's hypothesis is also consistent with what we know about the mechanics and molecular genetics of recombination, in particular the tendency for chromatids to recombine with a homolog rather than a sister chromatid at meiosis, which is opposite to what they do during mitosis. However, molecular genetic studies have shown that cis-acting sites at which recombination is initiated are lost by gene conversion as a result, a factor that can be expected to affect many fine details in the evolution of recombination. In summary, although Weismann's hypothesis must be considered the leading candidate for the function of sex and recombination, nevertheless, many additional principles are needed to fully account for their evolution.     

THE ETIOPLAST–CHLOROPLAST TRANSFORMATION IN TOBACCO: CORRELATION OF ULTRASTRUCTURE,REPLICATION, AND CHLOROPHYLL SYNTHESIS

Plastid ultrastructure was correlated with plastid replication and growth during the greening of etiolated tobacco tissue. The plastids grow rapidly, and this growth is correlated with a period of rapid chlorophyll synthesis. Chlorophyll synthesis is correlated with the formation of grana. The plastids divide by constriction and continue to do so after most of the grana have been formed. They contain single membrane-bound bodies, which are observed less frequently in the last stages of chloroplast maturation. No internal morphological features of the plastids appear to be related to causal aspects of replication.     

Fusobacterium nucleatum-associated β-Defensin Inducer (FAD-I): IDENTIFICATION,ISOLATION, AND FUNCTIONAL EVALUATION*

Human β-defensins (hBDs) are small, cationic antimicrobial peptides, secreted by mucosal epithelial cells that regulate adaptive immune functions. We previously reported that Fusobacterium nucleatum, a ubiquitous Gram-negative bacterium of the human oral cavity, induces human β-defensin 2 (hBD2) upon contact with primary oral epithelial cells. We now report the isolation and characterization of an F. nucleatum (ATCC 25586)-associated defensin inducer (FAD-I). Biochemical approaches revealed a cell wall fraction containing four proteins that stimulated the production of hBD2 in human oral epithelial cells (HOECs). Cross-referencing of the N-terminal sequences of these proteins with the F. nucleatum genome revealed that the genes encoding the proteins were FadA, FN1527, FN1529, and FN1792. Quantitative PCR of HOEC monolayers challenged with Escherichia coli clones expressing the respective cell wall proteins revealed that FN1527 was most active in the induction of hBD2 and hence was termed FAD-I. We tagged FN1527 with a c-myc epitope on the C-terminal end to identify and purify it from the E. coli clone. Purified FN1527 (FAD-I) induced hBD2 mRNA and protein expression in HOEC monolayers. F. nucleatum cell wall and FAD-I induced hBD2 via TLR2. Porphorymonas gingivalis, an oral pathogen that does not induce hBD2 in HOECs, was able to significantly induce expression of hBD2 in HOECs only when transformed to express FAD-I. FAD-I or its derivates offer a potentially new paradigm in immunoregulatory therapeutics because they may one day be used to bolster the innate defenses of vulnerable mucosae.     

CELL AND MOLECULAR BIOLOGY

One of the highest-ranking journals In biology with an Impact factor of 6.787! BioEssays Editor: Adam S. Wilkins, Cambridge, UK BioEssays offers readers in-depth review articles and serves as a resource for education and new ideas by exploring a wide range of biological topics. Researchers and teachers are presented with a unique tool for learning how findings in molecular, cellular,