清开灵致过敏性休克1例

黄某某,男,２３岁,因咳嗽、气喘、发热３ｄ就诊。发病３ｄ来曾在家自用过止咳喘及感冒药,病情未明显好转。既往有哮喘病史,１０岁以后好转。拟诊：（１）上呼吸道感染;（２）支气管哮喘。处理：给予先锋霉素Ｖ注射液４ｇ静滴;清开灵针４０ｍｌ静滴。（北京中医学院...     

人工栽培条件下三叶木通座果及果实生长特性研究

用栽培三叶木通为试材,研究了其座果及果实生长的特性。结果表明：人工栽培条件下,三叶木通座果（雌花）率可以提高到１３．５％,是野生三叶木通的２ １００％;以先年第１、２次攀援茎第３￣３０节位结果为主;３月底开花,花期３０ｄ左右。花后第２０￣５０ｄ（４月中旬至５月上旬）花序、雌花及幼果（子房）大量脱落,出现明显的脱落高峰。三叶木通果实纵向生长呈双Ｓ曲线布,生长期１５０ｄ左右,可划分成５个时期：受精结实     

小儿坏死性淋巴结炎2例

例１,女,１０岁。因反复发热４８ｄ,间有皮疹、咳嗽、眼结膜充血,经多种抗生素治疗无效于１９９５年８月１０日入院。检查：体温３８℃,一般情况好。左侧颈部可触及２个１ｃｍ×１．５ｃｍ淋巴结,左腋窝可触及３个约１．５ｃｍ×２ｃｍ淋巴结,均无压痛,可移动。心...     

蝮蛇抗栓酶治疗视网膜静脉阻塞8例

视网膜中央静脉阻塞是眼科的常见病,治疗方法很多。我院近几年用蝮蛇抗栓酶治疗视网膜静脉阻塞８例,疗效满意,现报告如下：１临床资料本组８例８眼,男７例７眼,女１例１眼,均为单眼发病,年龄１８～４８岁,发病到就诊时间：＜３ｄ２例,３～７ｄ５例,＞７ｄ１例,...     

吡喹酮涂肤剂预防及治疗小鼠血吸虫病的研究

本文报道了用不同剂型、不同浓度的吡喹酮涂肤剂预防及治疗小鼠日本血吸虫病的实验研究。作者选用乙醇、聚乙二醇４００和霜剂,将吡喹酮原剂放入其内使成１‰和２％浓度的吡喹酮涂肤剂。药物涂在鼠的腹部皮肤上。预防组在用药后１－６ｈ,洗净药物,感染血吸虫尾蚴６０±２条,感染后４２ｄ解剖小鼠,结果显示１‰吡喹酮乙醇液,聚乙二醇４００溶液和霜剂均有很好的预防效果,防护率为１００００％。治疗组用２％吡喹酮霜剂１次／ｄ,连续３－５ｄ,在用药后的１－４ｄ及４周,解剖小鼠,结果显示：对２１ｄ的血吸虫童虫的减虫率为４５４６％,对４２ｄ的血吸虫成虫的减虫率为７４６７％,提示２％吡喹酮涂肤剂抗日本血吸虫成虫的作用优于抗童虫的作用。     

玉米（Zea mays L.）cdc2和Prh1基因的染色体原位杂交物理定位

首次报道了玉米两个低拷贝基因ｃｄｃ２和ｐｒｈ１生物素标记的染色体原位杂交结果。供试探针为这两个基因的ｃＤＮＡ克隆ｃｄｃ２ＺｍＡ和ＺｍＰＰ１,其长度分别为１．３ｋｂ和１．７ｋｂ。结果表明,ｃｄｃ２ＺｍＡ的信号分布在第４、８和９染色体长臂,与着丝粒的百分距离分别为５７．８７±２．６８、２８．４２±１．４５和８８．１６±３．２８,检出率为１０．０７％．３．１３％和８．３３％。ｐｒｈ１ＺｍＰＰ１的信号分布在第４、６和８染色体长臂,与着丝粒的百分距离分别为５３．６２±１．１７．６０．７７±２．９０和１７．１０±１．６１,检出率为１２．０７％．５．１７％和６．４７％。对非放射性原位杂交技术以及基因ｃｄｃ２和ｐｒｈ１的物理位置与功能间的关系作了讨论。     

丙型肝炎病毒E2基因DNA免疫实验动物研究

将编码丙型肝炎病毒（ＨＣＶ）Ｅ２蛋白４１７～７５０位氨基酸的ＤＮＡ片段　克隆到真核表达载体ｐｃＤＮＡ　３．１（－）中的ＣＭＶ　ＩＥ启动子下游,构建成ＨＣＶ　Ｅ２重组真核表达质粒　ｐｃＥ２。ＥＬＩＳＡ法检测ｐｃＥ２　ＤＮＡ免疫兔血清中的Ｅ２抗体变化和维持规律,结果显示免疫２０ｄ已有　抗体产生,３０ｄ后开始进入高峰,４０ｄ时达到最高值,至第９０ｄ抗体水平保持平稳,抗体滴度　达到１∶１６００左右。流式细胞计数仪（ＦＡＣＳ）检测ｐｃＥ２　ＤＮＡ免疫鼠ＣＤ４＋、ＣＤ８＋Ｔ淋巴细胞变　化情况,与注射空载体ｐＣＤＮＡ３．１（－）的阴性鼠相比,ＣＤ４＋淋巴细胞水平略有上升,ＣＤ８＋　细胞水平有较大升高,增幅达３５．４６％。免疫组化检测结果显示注射ｐｃＥ２的小鼠组织中有明显　的阳性着色,而注射ｐｃＤＮＡ３．１（－）的对照组小鼠免疫组化结果为阴性。以上结果表明：ｐｃＥ２　在实验动物内表达出的ＨＣＶ　Ｅ２蛋白可以引起免疫动物的体液免疫应答和细胞免疫应答,尤其　是ＭＨＣ－１限制性杀伤性ＣＤ８＋Ｔ淋巴细胞水平的提高对清除　病毒是十分有利的,因此ＨＣＶ　Ｅ２　ＤＮＡ免疫有可能成为预防和治疗ＨＣＶ感染的一条新途径。     

大叶紫花苜蓿愈伤组织原生质体再生植株

大叶紫花苜蓿下胚轴诱导的愈伤组织在继代培养基上生长快速,易于分散。继代第１２ｄ的愈伤组织原生质体的得率为６．５×１０７／ｇ鲜重。原生质体培养基为ＳＨ基本培养基,含有１．０ｍｇ／Ｌ２,４－０、０．５ｍｇ／ＬＢＡ、２．０ｇ／ＬＣＨ、２％蔗糖、６％葡萄糖、５ｍｍｏｌ／ＬＭＥＳ,培养密度为１．０×１０５／ｍＬ。培养至第１２ｄ时的原生质体再生细胞植板率为３．７％。由原生质体形成的小愈伤组织在含２．０ｍｇ／Ｌ２,４－Ｄ的ＭＳ固体培养基上大量增殖。增殖的愈伤组织转移至２．０ｍｇ／Ｌ２－ｉｐ＋０．１ｍｇ／ＬＮＡＡ的Ｂ５培养基上,形成体细胞胚并发育成完整植株。     

笼养绿孔雀繁殖行为的观察

１　观察方法　３～５月在孔雀繁殖最活跃的季节,每周利用双休日带同学到动物园观察１ｄ,时间是８∶００～１９∶００,共观察１０ｄ。对动物园２号笼的４只成年绿孔雀（１∶３♀）进行重点观察,对其它笼内的孔雀作对照观察。采用目标动物取样法进行观察,电子计时器计时,观察绿孔雀的求偶行为,交尾行为等。２　观察结果２．１　繁殖期绿孔雀日活动时间分配（２４ｈ）　通过１０ｄ观察,繁殖期绿孔雀日活动时间分配见图１,图中绿孔雀静栖行为占６０．４２％,在时间分配中占的比例最大,求偶行为占１６．６７％,居第２位。说明进入…     

β1—4半乳糖基转移酶检测方法的建立及动力学研究

本文分离纯化了人ＩｇＧ的糖肽，去掉末端大酸和半乳糖，，经解和荧光标记，用ＨＰＬＣ纯化了荧光标记的底物［Ｇｎβ１－２ｍａ１－６（Ｇｎβ１－２Ｍｄα－３）Ｍβ１－４ｎβ１－４Ｇｎ－Ｐａ］。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.