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1.
阮松林  马华升  王世恒  忻雅  钱丽华  童建新  赵杭苹  王杰 《遗传》2006,28(12):1633-1648
蛋白质组技术已广泛应用于植物遗传、发育和生理生态等诸多生物学领域, 主要研究植物的遗传多样性、植物发育(如种子成熟与发芽过程)、组织器官的分化过程、不同亚细胞结构的新蛋白组分的发现及其功能鉴定、植物对非生物逆境(包括高温、低温、高盐和干旱等)和生物逆境(病虫害)的适应机制和植物与微生物(根瘤共生体)相互作用机制。同时对植物蛋白质组学的发展前景进行了讨论。  相似文献   

2.
生物质谱技术在蛋白质组学研究中的应用   总被引:2,自引:0,他引:2  
随着技术的进步,蛋白质组学的研究重心由最初旨在鉴定细胞或组织内基因组所表达的全部蛋白质转移到从整个蛋白质组水平上阐述包括蛋白翻译后修饰、生物大分子相互作用等反映蛋白质功能的层次。多种质谱离子化技术的突破使质谱技术成为蛋白质组学研究必不可少的手段。质谱技术联合蛋白质组学多角度、深层次探索生命系统分子本质成为现阶段生命科学研究领域的主旋律之一。本文简要综述了肽和蛋白质等生物大分子质谱分析的原理、方式和应用,并对其发展前景做出展望。  相似文献   

3.
生物质谱及其在蛋白质组学研究中的应用   总被引:1,自引:0,他引:1  
生物质谱是蛋白质组学研究必不可少的关键技术。近年来,生物质谱在鉴定通量、分辨率和灵敏度等方面均有质的飞跃,从而促进了蛋白质组研究各个领域的飞速发展。本文就生物质谱技术的原理、技术和仪器发展现状,及其在蛋白质组学研究中的应用进展作一简要的综述。  相似文献   

4.
植物蛋白质组学研究进展Ⅰ. 蛋白质组关键技术   总被引:10,自引:0,他引:10  
阮松林  马华升  王世恒  忻雅  钱丽华  童建新  赵杭苹  王杰 《遗传》2006,28(11):1472-1486
随着模式植物拟南芥和水稻基因组测序相继完成, 使植物基因组学研究成功迈入到功能基因组学研究的时代。这为蛋白质组学产生及其发展奠定了坚实的基础。文章重点介绍了蛋白质组学的概念、产生背景和蛋白质组学的关键技术。蛋白质组学的关键技术包括双向电泳、高效液相色谱、蛋白芯片、质谱技术、蛋白质组学的相关数据库、定量蛋白组技术、蛋白复合体标签亲和纯化技术和酵母双杂交系统。同时对当前蛋白质组技术面临的挑战和发展前景进行了讨论。  相似文献   

5.
植物蛋白质组学的研究取得了重要进展。全面介绍了植物器官蛋白质组、植物组织蛋白质组及植物亚细胞蛋白质组的研究进展。  相似文献   

6.
蛋白质组学是后基因组时代研究的热点领域之一,自从蛋白质组这个概念被提出以来,其研究一直受到广泛关注,其研究技术也有了极大地进步。植物时刻都面临各种非生物胁迫,包括干旱、冷、盐、金属等,在长期进化过程中,植物形成独特的机制来响应逆境,然而目前对于植物如何适应逆境的分子机制尚未完全阐明。因此蛋白质组学作为一种强有力的研究技术手段,将为研究植物响应胁迫的分子机制提供理论支撑。介绍了蛋白质组学的产生背景、研究技术手段及植物在各种胁迫条件下的蛋白质组学研究、植物亚细胞器的蛋白质组学研究状况,同时对植物蛋白质组学的发展前景进行了展望。  相似文献   

7.
蛋白质组学在研究植物响应逆境机理上的应用   总被引:1,自引:0,他引:1       下载免费PDF全文
徐刚  姚银安 《广西植物》2009,29(3):372-376
逆境条件下植物可以通过改变其基因表达和相关代谢活动来适应,探讨植物基因和蛋白表达谱的变化就成为植物逆境响应机制研究中的重要内容,蛋白质表达谱反映了植物细胞和组织的实际状态,是植物基因表达和最终代谢的关键环节。随着蛋白质分离技术、质谱鉴定技术和植物生物信息学的迅速发展,蛋白质组学在植物响应逆境方面的研究中的应用已经比较成功,加深了人们对植物响应逆境机制的认识,并为人们提供了新的线索和思维。本文主要对蛋白质组学在植物响应非生物逆境(干旱、盐胁迫、低温胁迫、高温胁迫等)和生物逆境(病虫害)的机制研究的应用上进行了综述。  相似文献   

8.
蛋白质组学以细胞、组织或器官表达的所有蛋白质为研究对象,通过双向凝胶电泳、生物质谱技术及生物信息学等方法,达到分离、鉴定、分析蛋白质的目的.肝癌发病过程涉及多基因的改变,必然伴随蛋白质表达的改变.以蛋白质组学的方法,通过比较正常肝组织与肝癌组织表达的蛋白质,寻找与肝癌发生、发展、转移及检测、治疗有关的生物分子.  相似文献   

9.
高通量植物蛋白质组学研究方法   总被引:2,自引:0,他引:2  
模式植物拟南芥和水稻的基因组测序,使得大规模、高通量的研究方法在基因组和蛋白质组研究中日趋重要。本文综述双向电泳、质谱、蛋白质微阵列、抗体、酵母双杂交系统以及一些新型高通量方法研究进展及其在植物蛋白质组研究中的应用。  相似文献   

10.
水稻病程相关蛋白质在逆境胁迫下的表达研究   总被引:1,自引:0,他引:1       下载免费PDF全文
植物病程相关(PR)基因一般在病原物侵染过程中受诱导发生转录上调.目前有证据提示植物PR基因在非生物逆境胁迫下也发生转录变化,但其蛋白质的表达变化情况还鲜有报道.为了解水稻PR蛋白质在逆境胁迫下的表达特征,本文采用免疫印迹技术(Western blotting,WB)调查了8个PR蛋白质在冷、热、旱、淹和盐等5种胁迫下的表达谱.结果表明:在冷胁迫下PR8表达上调,在热胁迫下PR1a、PR3、PR5和PR16表达下调;在旱胁迫下PR1a、PR2和PR8表达上调,而PR5 和PR16表达下调,在淹胁迫下PR1、PR2和PR15表达上调,PR1a、PR3、PR5和PR8表达下调;在盐胁迫下PR2和PR3表达上调,而PR1a、PR5、PR8和PR16表达下调.另外,对这些PR 基因的上游启动子区进行分析,发现存在与胁迫响应相关的调控元件,其中脱落酸反应元件(ABRE)、TC-rich repeats和HSE的出现频率较高.这些蛋白质表达数据进一步佐证了PR蛋白在逆境胁迫反应中发挥着重要且不尽相同的作用.  相似文献   

11.
    
《Journal of plant physiology》2014,171(15):1444-1451
  相似文献   

12.
13.
    
Gilbert S. Omenn 《Proteomics》2013,13(16):2375-2376
The human eye proteome is the latest addition to the HUPO Human Proteome Project (HPP). Semba et al. (The Human Eye Proteome Project: Perspectives on an emerging proteome. Proteomics 2013, 13, 2500–2511) establish a provisional baseline for the proteomes of the many anatomical compartments of the eye, based on literature review. As part of the Biology and Disease‐driven HPP, they and their colleagues will generate fresh data and meet the stringent guidelines for protein identification and characterization as established by the HPP.  相似文献   

14.
    
Twelve wild, endophyte-infected populations of perennial ryegrass were tested for resistance against artificial infection of Drechslera siccans and Fusarium spp. Plants with identified endophyte presence (E+), together with plants free from endophytes (E−), were inoculated with serious turf grass pathogens: D. siccans (cause of brown blight) and a mixture of Fusarium species (cause of Fusarium blight). For both diseases, the positive effect of endophyte presence on plant resistance was observed. In the case of a few ecotypes, endophyte infection increased resistance against both diseases, which is of practical importance for disease control.  相似文献   

15.
逆境胁迫对水稻DNA甲基化水平的影响   总被引:1,自引:0,他引:1  
植物在逆境胁迫下发生复杂的表现遗传变化,包括DNA甲基化、组蛋白修饰和RNA介导的基因沉默等.其中DNA甲基化是表现遗传学中的重要组成部分,主要通过甲基化、去甲基化来参与逆境胁迫下基因表达的调控,进而增强植物体的抗逆性,调节植物体的生长发育.就非生物与生物胁迫对水稻DNA甲基化水平的影响进行综述,为从表观遗传水平研究水稻抗逆性的机制提供理论参考.  相似文献   

16.
蛋白质组技术在鼠胚胎早期发育研究中的应用   总被引:2,自引:1,他引:2  
人类基因组大规模测序,揭示基因组精细结构的同时,还显示出基因数量的有限性和结构的相对稳定。随分析仪器和生物技术的飞速发展,创立了与基因组相对应的蛋白质组学,将精力集中于从生命功能的执行体---蛋白质水平研究基因的表达及功能。生殖技术的研究已取得了惊人的进展,但人们对生殖尤其是人类生殖的分子机制了解仍很贫乏。鼠胚胎发育过程蛋白质组的研究,为了解人类生殖健康和疾病发生的机制提供了有意义的资料 。  相似文献   

17.
    
Ossipova E  Fenyö D  Eriksson J 《Proteomics》2006,6(7):2079-2085
The two central problems in protein identification by searching a protein sequence collection with MS data are the optimal use of experimental information to allow for identification of low abundance proteins and the accurate assignment of the probability that a result is false. For comprehensive MS-based protein identification, it is necessary to choose an appropriate algorithm and optimal search conditions. We report a systematic study of the quality of PMF-based protein identifications under different sequence collection search conditions using the Probability algorithm, which assigns the statistical significance to each result. We employed 2244 PMFs from 2-DE-separated human blood plasma proteins, and performed identification under various search constraints: mass accuracy (0.01-0.3 Da), maximum number of missed cleavage sites (0-2), and size of the sequence collection searched (5.6 x 10(4)-1.8 x 10(5)). By counting the number of significant results (significance levels 0.05, 0.01, and 0.001) for each condition, we demonstrate the search condition impact on the successful outcome of proteome analysis experiments. A mass correction procedure utilizing mass deviations of albumin matching peptides was tested in an attempt to improve the statistical significance of identifications and iterative searching was employed for identification of multiple proteins from each PMF.  相似文献   

18.
Sriramulu DD  Nimtz M  Romling U 《Proteomics》2005,5(14):3712-3721
Pseudomonas aeruginosa is known for the chronic lung colonization of cystic fibrosis (CF) patients in addition to eye, ear and urinary tract infections. With the underlying disease CF patients are predisposed to P. aeruginosa chronic lung infection, which leads to morbidity and mortality. In this study, we compared the protein expression profile of a CF lung-adapted P. aeruginosa strain C with that of the burn-wound isolate PAO. Differentially expressed proteins from the whole-cell, membrane, periplasmic as well as extracellular fraction were identified. The whole-cell proteome of strain C showed down-regulation of several proteins involved in amino acid metabolism, fatty acid metabolism, energy metabolism and adaptation leading to a highly distinct proteome pattern for strain C in comparison to PAO. Analysis of secreted proteins by strain C compared to PAO revealed differential expression of virulence factors under non-inducing conditions. The membrane proteome of strain C showed modulation of the expression of porins involved in nutrient and antibiotic influx. The proteome of the periplasmic space of strain C showed retention of elastase despite that the equal amounts were secreted by strain C and PAO. Altogether, our results elucidate adaptive strategies of P. aeruginosa towards the nutrient-rich CF lung habitat during the course of chronic colonization.  相似文献   

19.
Shotgun proteomics is capable of characterizing differences in both protein quality and quantity, and has been applied in various biomedical applications. Unfortunately, the high complexity and dynamic range of proteins in studied samples, clinical in particular, often hinders the identification of relevant proteins. Indeed, information-rich, low abundance proteins often remain undetected, whereas repeatedly reported altered concentrations in high abundance proteins are often ambiguous and insignificant. Several techniques have therefore been developed to overcome this obstacle and provide a deeper insight into the proteome. Here we report a novel approach, which enables iTRAQ reagent quantitation of peptides fractionated based on presence of a cysteine residue (thus CysTRAQ). For the first time, we prove that iTRAQ quantitation is fully compatible with cysteinyl peptide enrichment and is not influenced by the fractionation process. Moreover, the employment of the method combined with high-resolution TripleTOF 5600 mass spectrometer for very fast MS/MS acquisition in human amniotic fluid analysis significantly increased the number of identified proteins, which were simultaneously quantified owing to the introduction of iTRAQ labeling. We herein show that CysTRAQ is a robust and straightforward method with potential application in quantitative proteomics experiments, i.e. as an alternative to the ICAT reagent approach.  相似文献   

20.
A reference database of the major soluble proteins of the primary root of the maize inbred line B73 was generated 5 days after germination (DAG) using a combination of 2-DE and MALDI-TOF MS. A total of 302 protein spots were detected with CBB in a pH 4-7 range and 81 proteins representing 74 distinct Genbank accessions were identified. Only 28% of the major proteins identified in 5 DAG primary roots were identified in similarly analyzed 9 DAG primary roots documenting remarkable changes in the accumulation of abundant soluble proteins early in primary root development.  相似文献   

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