双环醇片治疗慢性肝炎高转氨酶血症的疗效观察

目的：观察双环醇片治疗慢性肝炎高转氨酶血症的临床疗效。方法：选择住院或门诊慢性肝炎高转氨酶血症患者280例,随机分为治疗组和对照组,治疗组141例,对照组139例。治疗组在常规保肝治疗的基础上加用双环醇片25m每日3次口服,对照组在常规保肝治疗的基础上加用甘利欣胶囊150mg每日3次口服。疗程均为4周。治疗前后每周详细记录患者症状、体征、肝功能、肾功能、电解质、及血尿常规,同时记录治疗过程中的不良反应及停药后随访3个月。结果：两组均有显著疗效,肝功能生化指标与治疗前相比有显著性差异（治疗组P〈0.01,对照组P〈0.05）,治疗组明显优于对照组（P〈0.05）,且治疗组出现的不良反应明显少于对照组。结论：双环醇片治疗慢性肝炎高转氨酶血症疗效好,不良反应较少,值得临床推广。     

源首胶囊治疗新生儿高胆红素血症疗效观察

目的及早有效地纠正新生儿高胆红素血症,预防胆红素脑损伤。方法将98例患儿随机分为2组。治疗组在常规治疗的同时加用源首胶囊口服。结果出院时治疗组胆红素含量(60.23±34.82)μm o l/L,明显低于对照组(89.47±36.76)μm o l/L(P<0.05);治愈天数治疗组(5.23±1.62)d明显低于对照组(6.92±1.79)d(P<0.01)。结论源首胶囊治疗新生儿高胆红素血症,可迅速降低血胆红素水平,明显缩短治疗时间,且安全性高,无副作用。     

蝮蛇抗栓酶胶囊治疗高粘血症187例疗效观察

1998年以来 ,我院应用蝮蛇抗栓酶胶囊 (下称抗栓酶胶囊 )治疗高粘血症 1 87例 ,疗效显著 ,现总结报告如下。1　临床资料1 .1　一般资料　本组 1 87例均为门诊病人 ,其中男 1 3 2例 ,女 5 5例 ;年龄 3 9～ 74岁 ,其中年龄 3 9～ 49岁 78例 (4 1 .7% ) ,5 0～ 69岁 85例 (4 5 .5 % ) ,70岁以上 2 4例 (1 2 .8% ) ;病程最短 3个月 ,最长3年 ,多为长时间服用其它药物无效者。其中有脑动脉硬化 78例 ,有高血压病史 1 2 5例 ,有冠心病2 5例。均经查血液流变学检测确诊。1 .2　治疗方法　应用蝮蛇抗栓酶 0 .5～ 0 .75 u (2～ 3支 )改装入肠溶胶囊中…     

胆红素血浆吸附治疗高胆红素血症疗效观察

我院1999年10月至2003年12月先后对32例高胆红素血症的重型肝炎患者进行胆红素血浆吸附治疗,在加快黄疸消退,提高患者生存率方面取得了较好的效果.现报告如下.     

富马酸替诺福韦二吡呋酯片联合双环醇在治疗慢性乙型病毒性肝炎方面的研究

目的:研究探讨富马酸替诺福韦二吡呋酯片(tenofovir dipivoxil fumarate tablets,TDF)联合双环醇在治疗慢性乙型病毒性肝炎方面的临床效果.方法:选取2017年1月-2020年1月中国人民解放军陆军第七十三集团军医院疾病预防控制科感染病区收治的80例慢性乙型病毒性肝炎患者,随机将其分为两...     

降纤酶治疗高粘血症40例报告

目的：观察降纤酶治疗高粘血症患者的临床疗效。方法用生理盐水２５０ｍｌ加降纤酶５ｕ,体重超过６５ｋｇ和１０ｕ,静脉滴注,每天１次,连用３天,第４天停用,第５天开始隔天静脉滴注降纤酶５ｕ或者１０ｕ,总量６０ｕ。结果降纤酶能明显地降低高粘血症患者的血液粘度、纤维蛋白原、红细胞聚集指数、微循环滞留时间等,同时也能使患者原有临床症状和体征以及微循环得到明显改善,结论降纤酶是治疗高粘血症的有效药物。     

中药大黄敷脐治疗新生儿高胆红素血症的临床观察

目的观察中药大黄敷脐治疗新生儿高胆红素血症的临床疗效。方法将100例高胆红素血症新生儿随机分为观察组和对照组,对照组50例采取常规治疗,观察组50例在常规治疗基础上加用中药大黄敷脐。结果两组患儿治疗前胆红素值比较无统计学意义(P0.05);治疗后两组患儿每天胆红素下降值、降至正常范围所需时间比较,差异有统计学意义(P0.05)。结论在常规治疗基础上加用中药大黄敷脐治疗新生儿高胆红素血症可迅速降低血清胆红素水平,疗效显著,是一种可靠的治疗方法。     

金双歧治疗新生儿高胆红素血症的临床疗效研究

目的 探讨微生态制剂金双歧(双歧杆菌乳酸杆菌三联活菌片)对新生儿高胆红素血症的临床疗效,以找出能及早有效地纠正新生儿高胆红素血症,预防新生儿胆红素脑病发生的新方法.方法 通过随机抽样、收集青白江区人民医院2007年3月至2011年3月72例新生儿高胆红素血症患儿的临床资料并进行回顾性分析,将72例新生儿高胆红素血症患儿随机分为治疗组40例和对照组32例.对照组给予常规治疗,治疗组在常规治疗基础上,加用金双歧1片(0.5亿)/次,每天3次,温开水溶碎后喂服或经胃管注入,共7～10d.检测治疗前后两组血清胆红素水平、计算日平均经皮测胆红素下降值、平均住院时间和黄疸消退时间.结果 金双歧辅助治疗新生儿高胆红素血症,治疗后治疗组与对照组血清总胆红素水平分别为(58.21 +4.36) μmol/L和(64.16±5.29) μmol/L(P ＜0.01);治疗组和对照组日平均经皮测胆红素下降值、平均住院时间和黄疸退尽平均时间分别为( 10.35±2.46)、(7.68±1.35)μmol/L;( 6/54±2/59)、(9/05 +3/12)d; (6/38±1/38)、( 8/75±2/68)d;差异均有统计学意义(P＜0.01).结论 应用金双歧治疗新生儿高胆红素血症可迅速降低血胆红素水平,缩短治疗时间,预防胆红素脑病的发生疗效确切、安全,可作为治疗新生儿高胆红素血症的方法之一,值得临床应用推广.     

阿托伐他汀钙片对高胆固醇血症的降脂疗效的临床观察

本文主要通过对高胆固醇血症患者进行不则剂量的阿托伐他汀钙片分组治疗,从而验证不同剂量的阿托伐他汀钙片对高胆固醇血症的降脂疗效,实验结果显示较高剂量(60mg/日)的阿托伐他汀钙片对高胆固醇血症的治疗具有较理想的效果。     

中药当归枸杞子汤调节高泌乳素血症临床观察

目的通过连续动态监测泌乳素血液浓度,评价当归枸杞子汤对高泌乳素血症的调节作用。方法选择我院2012年5月~2013年5月门诊收治的高泌乳素血症患者130例,随机分为对照组30例,采用溴隐亭治疗;观察组100例,给予中药当归枸杞子汤治疗。连续动态监测血清泌乳素浓度,评价两组疗效。结果两组患者疗效比较无显著统计学意义(P0.05)。两组治疗后血清泌乳素均显著下降,与治疗前比较差异有统计学意义(P0.05);而两组治疗后比较差异无统计学意义(P0.05)。不良反应发生率比较,对照组显著高于观察组(P0.05)。结论中药当归枸杞子汤治疗高泌乳素血症安全、有效,且价格低廉,用法简单,有显著的临床推广应用价值。     

谷氨酸依赖型氨基转移酶的高通量筛选方法及其应用

目的:建立谷氨酸依赖型氨基转移酶-谷氨酸脱氢酶偶联反应的96孔板高通量筛选方法,并用于大肠杆菌氨基转移酶Wec E突变库的筛选。方法:通过优化偶联指示酶-谷氨酸脱氢酶、信号分子NADH浓度及双酶偶联反应时间,建立了光学法测定氨基转移酶活性的氨基转移酶-谷氨酸脱氢酶偶联反应方法;通过定点饱和突变技术构建了大肠杆菌氨基转移酶WecE的突变库;采用96孔板高通量初筛、摇瓶复筛获得了高活性的转氨酶突变体,并对纯化的突变体进行催化活力分析。结果:建立了谷氨酸依赖型氨基转移酶目标反应与0.5 U/ml L-谷氨酸脱氢酶和0.4 mmol/L NADH信号指示反应相偶联的筛选方法;构建了氨基转移酶WecE Tyr 321饱和突变库,通过96孔板高通量筛选,获得了催化活性比野生型提高3.4倍的突变体Y321F。结论:所建立高通量筛选方法背景干扰小,准确性高,为谷氨酸依赖型氨基转移酶分子进化提供了可行性方案。     

Transaminase activity rhythms in Penicillium claviforme

Abstract

A photoinduced endogenous rhythm of zonation has been described by Jerebzoff and Piskorz‐Binczycka (1987) and Piskorz‐Binczycka et al. (1989) in the fungus Pénicillium claviforme Bainier CBS strain 126–23 ; a 24–26 h periodicity was elicited by and displayed in continuous white light. The present work shows that aspartate and alanine aminotransferase activities oscillated in continuous white light (fluence rate: 1.5 mW m^‐2), in synchronism, with a period of about 24h.     

Purification and properties of alanine aminotransferase from maize (Zea mays L.) leaves

Alanine aminotransferase (AlaAT, EC 2.6.1.2) from leaves of 14-day-old maize seedlings was purified over 1600-fold to electrophoretical homogeneity. Specific activity of the purified enzyme measured with L-alanine and 2-oxoglutarate as substrates was 2125 nkat·(mg protein)⁻¹ at 30 °C. The molecular weights of the native and sodium dodecyl sulfate — denatured AlaAT protein were 95 kDa and 50 kDa respectively, indicating that the native enzyme is probably a homodimer. AlaAT almost exclusively catalyzed amino group transfer from L-alanine to 2-oxoglutarate and the reverse reaction. The inhibitory experiments showed that pirydoxal phosphate is directly involved in the enzymatic catalysis and the enzyme molecule contains essential SH groups. The use of phenylglyoxal demonstrated the presence of arginine residue as anionic binding site in the active centre of AlaAT. This work was supported by the State Committee for Scientific Research, a grant No. 5PO6A00510     

Determination of bicyclol in dog plasma using liquid chromatography–tandem mass spectrometry

A sensitive and accurate method for determination of bicyclol in dog plasma was developed. Thermo Scientific TSQ Quantum triple quadrupole system with multiple ion monitoring (MIM) positive scanning mode was applied. Bicyclol and DDB (IS) sodium adduct molecular ions were monitored at m/z 413 and m/z 441 in both Q1 and Q3, respectively. The collision energy in Q2 was set to 15 eV. Precipitation method was employed in the extraction of bicyclol and DDB from the biological matrix. The method was validated over 1–500 ng/mL for bicyclol. The recovery was 96.5–109.5%, and the limit of quantitation (LOQ) detection was 1 ng/mL for bicyclol. The intra- and inter-day precision of the method at three concentrations was 3.3–14.3% with accuracy of 99.9–109.0%. The method was successfully applied to bioequivalence studies of bicyclol controlled-release formulation to obtain the pharmacokinetic parameters.     

Aspartate and Alanine Aminotransferases in Early Development of the Keta

We studied the activities of the marker enzymes of physiological state and adaptive reactions, aspartate and alanine aminotransferases, in early development of the keta Oncorhynchus keta. Aspartate aminotransferase with pH optima 6.8, 7.0, 7.6, and 8.0 and alanine aminotransferase with pH optima 7.0, 7.4, 7.6, 7.8, 8.0, and 8.2 were found in the eggs, larvae, and fry. The succession of enzymes with different pH takes place during ontogenesis, as well as stage specific changes in their activity. The maximum enzymatic activity was recorded in the larvae during their rise for afloat. A correlation was established between the dynamics of enzymatic activity and soluble nitrogen and amine nitrogen contents.     

The use of natural and unnatural amino acid substrates to define the substrate specificity differences of Escherichia coli aspartate and tyrosine aminotransferases.

The tyrosine (eTATase) and aspartate (eAATase) aminotransferases of Escherichia coli transaminate diacarboxylic amino acids with similar rate constants. However, eTATase exhibits approximately 10(2)-10(4)-fold higher second-order rate constants for the transamination of aromatic amino acids than does eAATase. A series of natural and unnatural amino acid substrates was used to quantitate specificity differences for these two highly related enzymes. A general trend toward lower transamination activity with increasing side-chain length (extending from aspartate to glutamate to alpha-aminoadipate) is observed for both enzymes. This result suggests that dicarboxylate ligands associate with the two highly related enzymes in a similar manner. The high reactivity of the enzymes with L-Asp and L-Glu can be attributed to an ion pair interaction between the side-chain carboxylate of the amino acid substrate and the guanidino group of the active site residue Arg 292 that is common to both enzymes. A strong linear correlation between side-chain hydrophobicity and transamination rate constants obtains for n-alkyl side-chain amino substrates with eTATase, but not for eAATase. The present kinetic data support a model in which eAATase contains one binding mode for all classes of substrate, whereas the active site of eTATase allows an additional mode that has increased affinity for hydrophobic amino acid.