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1.
We studied how the relationship between cadmium (Cd) toxicity and oxidative stress influenced the growth, photosynthetic efficiency,
lipid peroxidation, and activity of ntioxidative enzymes in the roots and leaves of rice(Oryza sativa L Dongjin). Plants were exposed to Cd for 21 d. Both seedling growth and photosynthetic efficiency decreased gradually with
increasing cadmium concentrations. Lipid peroxidation increased slowly in both roots and leaves, causing oxidative stress.
However, each tissue type responded differently to Cd concentrations with regard to the induction/ inhibition of antioxidative
enzymes. The activity of Superoxide dismutase (SOD) increased in both roots and leaves. Ascorbate peroxidase (APX) activity
increased in leaves treated with up to 0.25 μM Cd, then decreased gradually at higher concentrations. In contrast, APX activity
in roots increased and remained constant between 0.25 and 25 μM Cd. Enhanced peroxidase (POD) activity was recorded for treatments
with up to 25/M Cd, gradually decreasing at higher concentrations in the leaves but remaining unchanged in the roots. Catalase (CAT) activity
increased in the roots, but decreased in the leaves, whereas the activity of glutathione reductase (GR) was enhanced in both
roots and leaves, where it remained elevated at higher Cd concentrations. These results suggest that rice seedlings tend to
cope with free radicals generated by Cd through coordinated, enhanced activities of the antioxidative enzymes involved in
detoxification. 相似文献
2.
Cadmium-induced oxidative damage and antioxidative defense mechanisms in <Emphasis Type="Italic">Vigna mungo</Emphasis> L. 总被引:1,自引:1,他引:0
Alicia S. Molina Carlos Nievas María Verónica Pérez Chaca Francisco Garibotto Ulises González Silvana M. Marsá Celina Luna María Sofía Giménez Fanny Zirulnik 《Plant Growth Regulation》2008,56(3):285-295
Cadmium (Cd)-induced oxidative stress and antioxidant defense mechanisms were analyzed in roots and leaves of Vigna mungo L. Seeds were germinated in perlite-vermiculite and irrigated with Hoagland nutrient solution. At day 6, seedlings were exposed
to 40 μM Cd under semi-hydroponic conditions for a period of 12 days. Growth anomalies and abnormal chromatin condensation
were observed in Cd-treated plants, in comparison with control ones. Cd accumulation was observed in roots of treated plants.
The analyses of antioxidative defense and oxidative parameters in roots, stems and leaves showed different tissue-specific
responses. Superoxide dismutase (SOD) and guaiacol peroxidase (GPx) activities and the level of lipid peroxidation (MDA content)
decreased in roots. However, they increased in leaves. Catalase activity and chlorophyll content, on the other hand, decreased
over exposure to Cd stress. Total glutathione, non-protein thiols, reduced glutathione (GSH) and phytochelatins increased
significantly, while oxidized glutathione (GSSG) decreased, as compared with control plants. The present data suggest that
the presence of Cd in soil and water can cause oxidative damage that may be detrimental for optimum production of nutritional
mung. 相似文献
3.
Treatment of tomato seedlings (Lycopersicon esculentum Mill. cv. 63/5 F1) with increasing CdCl2 concentrations in the culture medium resulted in Cd accumulation more important in roots than in leaves. Biomass production
was severely inhibited, even at low Cd concentration. Cd reduced chlorophyll content in leaves and enhanced lipid peroxidation.
An increase in antioxidative enzyme (superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase)
activities was more pronounced in leaves than in roots, while catalase activity increased only in roots. In addition, changes
in isoenzyme composition were observed using the non-denaturing polyacrylamid gel electrophoresis. 相似文献
4.
J. F. Gonçalves L. A. Tabaldi D. Cargnelutti L. B. Pereira J. Maldaner A. G. Becker L. V. Rossato R. Rauber M. D. Bagatini D. A. Bisognin M. R. C. Schetinger F. T. Nicoloso 《Biometals》2009,22(5):779-792
A hydroponic experiment was carried out to characterize the oxidative stress responses of two potato cultivars (Solanum tuberosum L. cvs. Asterix and Macaca) to cadmium (Cd). Plantlets were exposed to four Cd levels (0, 50, 100, 150 and 200 μM) for 7 days.
Cd concentration was increased in both roots and shoot. Number of sprouts and roots was not decreased, whereas Cd treatment
affected the number of nodal segments. Chlorophyll content and ALA-D activity were decreased in both cultivars, whereas carotenoids
content was decreased only in Macaca. Cd caused lipid peroxidation in roots and shoot of both cultivars. Protein oxidation
was only verified at the highest Cd level. H2O2 content was increased in roots and shoot of Asterix, and apparently, a compensatory response between roots and shoot of Macaca
was observed. SOD activity was inhibited in roots of Asterix at all Cd treatments, whereas in Macaca it was only increased
at two highest Cd levels. Shoot SOD activity increased in Asterix and decreased in Macaca. Root CAT activity in Asterix decreased
at 100 and 150 μM, whereas in Macaca it decreased only at 50 μM. Shoot CAT activity was decreased in Macaca. Root AsA content
in Macaca was not affected, whereas in shoot it was reduced at 100 μM and increased at 200 μM. Cd caused increase in NPSH
content in roots and shoot. Our results suggest that Cd induces oxidative stress in both potato cultivars and that of the
two cultivars, Asterix showed greater sensitivity to Cd levels. 相似文献
5.
Characteristics of cadmium (Cd) accumulation and tolerance in Rorippa globosa (Turcz.) Thell., a species with some characteristics of cadmium hyperaccumulation were further investigated and compared
with a closely related species, Rorippa islandica. The results showed that there was no phytotoxicity for R. globosa leaves or reduction in biomass when treated with 25 μg Cd g−1, although the concentration of Cd accumulated in the leaves was up to 218.9 μg Cd g−1 dry weight (DW). On the contrary, Cd toxicity was observed in R. islandica leaves by way of determining changes in fresh weight (FW), malondialdehyde (MDA) level and chlorophyll content while treated
with 25 μg Cd g−1 DW. R. globosa had stronger self-protection ability than R. islandica to adapt to oxidative stress caused by Cd. Application of Cd significantly increased the activity of superoxide dismutase
(SOD) in leaves, the activity of peroxidase (POD) in roots, and the activity of catalase (CAT) in leaves and roots of R. globosa. By contrast, in R. islandica, the activity of antioxidant enzymes was inhibited or unchanged by various Cd treatments. However, R. globosa leaves had higher activity of antioxidant enzymes such as SOD and POD than that of R. islandica. The antioxidative defense systems in R. globosa might play an important role in Cd tolerance. The Cd treatments significantly induced the synthesis of phytochelatins (PCs)
in the two species. Leaf PCs and Cd accumulation by R. globosa were much greater than those by R. islandica, but root PCs and Cd accumulation by R. islandica were much greater than those by R. globosa, suggesting that PCs in leaves may be a biomarker of Cd hyperaccumulation, and the synthesis of PCs may be related to an
increase in the uptake of Cd ions into the cytoplasm, not the primary mechanism for Cd tolerance. 相似文献
6.
Hydroponic experiments were carried out to study the role of alginate-derived oligosaccharides (ADO) in enhancing wheat (Triticum aestivum L.) tolerance to cadmium stress. Data were collected on plant biomass, chlorophyll content, photosynthetic rate, antioxidant
enzyme activity and malondialdehyde (MDA) content. Under 100 μM Cd stress, plant growth was significantly inhibited. Shoot
length, root length, fresh and dry weight were sharply reduced by 24.21, 34.59, 22.1 and 14.7%, respectively of the control
after 10 day of Cd exposure. Superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were increased and
MDA content increased. Wheat seeds were soaked for 5 h in 1,000 mg L−1 ADO solution before cadmium stress. ADO pretreatment alleviated cadmium toxicity symptoms, which were reflected by increasing
root and shoot lengths, fresh and dry weight, chlorophyll content and photosynthetic rate (P
n
). Furthermore, ADO pretreatment significantly increased antioxidant enzyme (SOD, CAT and POD) activities and reduced MDA
content in leaves and roots. The results indicated that ADO pretreatment partially protected the seedlings from cadmium toxicity
during the following growth period. 相似文献
7.
The role of salicylic acid (SA) in alleviating cadmium (Cd) toxicity was investigated in a hydroponic cultivation system. Short-term exposure of bean (Phaseolus vulgaris) plants to 20 μM Cd inhibited biomass production and intensively increased accumulation of Cd in both roots and leaves. At leaf level, Cd significantly decreased mineral ions, chlorophyll and carotenoids concentrations. Concomitantly, Cd enhanced electrolyte leakage, H2O2 content and lipid peroxidation as indicated by malondialdehyde (MDA) accumulation. SA pretreatment decreased the uptake and the transport of Cd, alleviated the Cd-induced inhibition of nutrient absorption and led to a significant increase of chlorophyll and carotenoid content. SA application alleviated the oxidative damages as evidenced by the lowered H2O2 and MDA content. SA particularly induced an increase in both CAT and APX activities accompanied by a significant reduction in SOD and POD activities. As important antioxidants, ascorbate and glutathione contents in bean leaves exposed to cadmium were significantly decreased by SA treatment. These results reveal the potentiating effect of salicylic acid in regulating cadmium induced oxidative stress in bean plants. 相似文献
8.
Thirty-day-old seedlings of tomato (Lycopersicon esculentum Mill.) were treated with various Hg concentrations (0, 10, and 50 μM) for up to 20 days, and the hypothesis that Hg induces oxidative stress leading to the reduction of biomass and chlorophyll content in leaves was examined. The accumulation of Hg in seedlings increased with external Hg concentration and exposure time, and Hg content in roots exposed to 50 μM Hg for 20 days was about 27-fold higher than that in shoots. Furthermore, Hg exposure not only reduced biomass and chlorophyll levels in leaves but also caused an overall increase of endogenous H2O2, lipid peroxidation products (malondialdehyde), and antioxidant emzymes activities such as superoxide dismutase, catalase, and peroxidase in leaves and roots. Our results suggest that the suppression of growth and the reduction of chlorophyll levels in tomato seedlings exposed to toxic Hg levels may be caused by an enhanced production of active oxygen species and subsequent high lipid peroxidation. 相似文献
9.
Iron nutrition affects cadmium accumulation and toxicity in rice plants 总被引:12,自引:0,他引:12
Guosheng Shao Mingxue Chen Weixia Wang Renxiang Mou Guoping Zhang 《Plant Growth Regulation》2007,53(1):33-42
The effect of iron (Fe) nutrition on cadmium (Cd) toxicity and accumulation in rice plants was studied using a hydroponic
system. The inhibitory effect of Cd on plant growth and chlorophyll content (SPAD value) was dependent on Fe level and the
genotype. Malondialdehyde (MDA) content in leaves and roots was not much affected by an increased Cd stress at 0.171 mg l−1 Fe, but it showed a rapid increase when the plants were exposed to moderate (1.89 mg l−1) and high (16.8 mg l−1) Fe levels. High Fe nutrition caused a marked reduction in Cd content in both leaves and roots. Fe content in plants was
lower at high Cd (5.0 μM) stress than at low Cd (<1.0 μM) stress. Cd stress increased both superoxide dismutase (SOD) and
peroxidase (POD) activities at low and moderate Fe levels. However, with high Fe level, it increased the POD activity, but
reduced the SOD activity. Our results substantiate the hypothesis that cell membrane-bound iron transporter (carrier) involved
in high-affinity iron transport systems can also transport Cd, and both these ions may compete for this common carrier. The
study further showed that there were significant correlations between MDA and Fe contents in leaves and roots of rice plants.
It is suggested that the occurrence of oxidative stress in plants exposed to Cd stress is mediated by Fe nutrition. The present
results also show that Cd stress affects the uptake of Cu and Zn. 相似文献
10.
Fei Chen Fang Wang Hongyan Sun Yue Cai Weihua Mao Guoping Zhang Eva Vincze Feibo Wu 《Journal of Plant Growth Regulation》2010,29(4):394-408
A greenhouse hydroponic experiment was performed using Cd-sensitive (cv. Dong 17) and Cd-tolerant (Weisuobuzhi) barley seedlings
to evaluate how different genotypes responded to cadmium (Cd) toxicity in the presence of sodium nitroprusside (SNP), a nitric
oxide (NO) donor. Results showed that 5 μM Cd increased the accumulation of O2•−, H2O2, and malondialdehyde (MDA) but reduced plant height, chlorophyll content, net photosynthetic rate (P
n), and biomass, with a much more severe response in the Cd-sensitive genotype. Antioxidant enzyme activities increased significantly
under Cd stress in the roots of the tolerant genotype, whereas in leaves of the sensitive genotype, superoxide dismutase (SOD)
and ascorbate peroxide (APX), especially cytosol ascorbate peroxidase (cAPX), decreased after 5–15 days Cd exposure. Moreover,
Cd induces NO synthesis by stimulating nitrate reductase and nitric oxide synthetase-like enzymes in roots/leaves. A Cd-induced
NO transient increase in roots of the Cd-tolerant genotype might partly contribute to its Cd tolerance. Exogenous NO dramatically
alleviated Cd toxicity, markedly diminished Cd-induced reactive oxygen species (ROS) and MDA accumulation, ameliorated Cd-induced
damage to leaf/root ultrastructure, and increased chlorophyll content and P
n. External NO counteracted the pattern of alterations in certain antioxidant enzymes induced by Cd; for example, it significantly
elevated the depressed SOD, APX, and catalase (CAT) activities in the Cd-sensitive genotype after 10- and 15-day treatments.
Furthermore, NO significantly increased stromal APX and Mn-SOD activities in both genotypes and upregulated Cd-induced decrease
in cAPX activity and gene expression of root/leaf cAPX and leaf CAT1 in the Cd-sensitive genotype. These data suggest that under Cd stress, NO, as a potent antioxidant, protects barley seedlings
against oxidative damage by directly and indirectly scavenging ROS and helps to maintain stability and integrity of the subcellular
structure. 相似文献
11.
Parsley (Petroselinum hortense L.) plants cultivated under controlled conditions were exposed to different doses of cadmium to investigate the antioxidant capacity and cadmium accumulation in the samples. Two-months-old parsley seedlings were treated with four different concentrations of CdCl2 (0, 75, 150, and 300 μM) for fifteen days. Cadmium level in leaves increased significantly by increasing the Cd contamination in the soil. Total chlorophyll and carotenoid content declined considerably with Cd concentration. Cd treatment caused a significant increase lipid peroxidation in tissue of leaf. Superoxide dismutase activity (SOD, EC 1.15.1.1) increased partially at 75 and 150 μM CdCl2 concentrations whereas the activity decreased at 300 μM CdCl2. Catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were reduced by Cd application. Total phenolic compound amount increased significantly with increasing Cd concentration, as ferric reduction power, superoxide anion radical, and DPPH˙ free radical scavenging activities elevated slightly by the concentration. These results suggest that antioxidant enzymes activities were repressed depending on accumulation of cadmium in leaves of parsley while the non-enzymatic antioxidant activities slightly increased. 相似文献
12.
The effect of calcium (Ca2+) on Trifolium repens L. seedlings subjected to cadmium (Cd2+) stress was studied by investigating plant growth and changes in activity of antioxidative enzymes. Physiological analysis
was carried out on seedlings cultured for 2 weeks on half-strength Hoagland medium with Cd2+ concentrations of 0, 400 and 600 μM, and on corresponding medium supplied with CaCl2 (5 mM). Exposure to increasing Cd2+ reduced the fresh weight of the upper part (stems + leaves) of the seedlings more strongly than that of the root system.
In both parts of T. repens seedlings H2O2 level and lipid peroxidation increased. In the upper part, Cd2+ exposure led to a significant decrease in the activity of superoxide dismutase, catalase and glutathione peroxidase and an
increase in ascorbate peroxidase activity. In contrast, the roots showed an increase in the activity of antioxidative enzymes
under Cd2+ stress. Ca2+ addition to medium reduced the Cd2+ accumulation, and considerably reversed the Cd2+-induced decrease in fresh mass as well as the changes in lipid peroxidation in the both parts of T. repens seedlings. Ca2+ application diminished the Cd2+ effect on the activity of antioxidative enzymes in the upper part, even though it did not significantly affect these enzymes
in the roots. So the possible mechanisms for the action of Ca2+ in Cd2+ stress were considered to reduce Cd2+ accumulation, alleviate lipid peroxidation and promote activity of antioxidative enzymes. 相似文献
13.
Cadmium-induced initial changes in the production of reactive oxygen species (ROS) and antioxidant mechanism were investigated
in soybean (Glycine max L. cv. Don Mario 4800 RR) leaves. Whole plants (WP) and plants without roots (PWR) were exposed to 0.0, 10.0 and 40.0 μM
Cd for 0, 4, 6 and 24 h. Compared to PWR, a higher level of endogenous Cd in WP was associated with a lower oxidative stress
measured in terms of lipid peroxidation. Furthermore, O2
•− content decreased in the leaves of Cd-treated WP, whereas it increased in those of Cd-treated PWR. Although O2
•− accumulation in PWR was associated with a decrease in superoxide dismutase (SOD) activity, O2
•− diminution in WP leaves was not related to any increase in SOD activity. H2O2 content increased in the leaves of both Cd-treated WP and PWR, and it was concomitant with a corresponding decline in catalase
(CAT) and ascorbate peroxidase (APX) activities. When diphenyl iodonium (DPI), an inhibitor of NADPH oxidase, was added, H2O2 content remained unchanged in Cd-treated WP, suggesting that NADPH oxidase does not participate in the early hours of Cd
toxicity. Taken together, our results showed that early ROS evolution and oxidative damage were different in WP and PWR. This
suggests that the response in soybean leaves during the early hours of Cd toxicity is probably modulated by the root. 相似文献
14.
Issam Nouairi Wided Ben Ammar Nabil Ben Youssef Douja Daoud Ben Miled Mohamed Habib Ghorbal Mokhtar Zarrouk 《Acta Physiologiae Plantarum》2009,31(2):237-247
Plant species capable of hyper-accumulating heavy metals are of considerable interest for phytoremediation, and differ in
their ability to accumulate metals from environment. Using two brassica species (Brassica juncea and Brassica napus), nutrient solution experiments were conducted to study variation in tolerance to cadmium (Cd) toxicity based on (1) lipid
peroxidation and (2) changes in antioxidative defense system in leaves of both plants (i.e., superoxide dismutase (SOD EC
1.15.1.1), catalase (CAT EC 1.11.1.6), ascorbate peroxidase (APX EC 1.11.1.11), guaiacol peroxidase (GPX EC 1.11.1.7), glutathione
reductase (GR EC 1.6.4.2), levels of phytochelatins (PCs), non-protein thiols (NP-SH), and glutathione. Plants were grown
in nutrient solution under controlled environmental conditions, and subjected to increasing concentrations of Cd (0, 10, 25
and 50 μM) for 15 days. Results showed marked differences between both species. Brassica napus under Cd stress exhibited increased level of lipid peroxidation, as was evidenced by the increased malondialdehyde (MDA)
content in leaves. However, in Brassica
juncea treated plants, MDA content remained unchanged. In Brassica napus, with the exception of GPX, activity levels of some antioxidant enzymes involved in detoxification of reactive oxygen species
(ROS), including SOD, CAT, GR, and APX, decreased drastically at high Cd concentrations. By contrast, in leaves of Brassica juncea treated plants, there was either only slight or no change in the activities of the antioxidative enzymes. Analysis of the
profile of anionic isoenzymes of GPX revealed qualitative changes occurring during Cd exposure for both species. Moreover,
levels of NP-SH and PCs, monitored as metal detoxifying responses, were much increased in leaves of Brassica juncea by increasing Cd supply, but did not change in Brassica napus. These results indicate that Brassica juncea plants possess the greater potential for Cd accumulation and tolerance than Brassica napus. 相似文献
15.
Effects of exogenous nitric oxide on cadmium toxicity, element contents and antioxidative system in perennial ryegrass 总被引:2,自引:0,他引:2
Quanhui Wang Xue Liang Yuanjie Dong Linlin Xu Xiuwei Zhang Jun Hou Zhenyi Fan 《Plant Growth Regulation》2013,69(1):11-20
The effects of sodium nitroprusside (SNP, a donor of NO) on cadmium (Cd) toxicity in ryegrass seedlings (Lolium perenne L.) were studied by investigating the symptoms, plant growth, chlorophyll content, lipid peroxidation, H+-ATPase enzyme and antioxidative enzymes. Addition of 100???M CdCl2 caused serious chlorosis and inhibited the growth of ryegrass seedlings, and dramatically increased accumulation of Cd in both shoots and roots, furthermore, the absorption of macro and micronutrients were inhibited. Addition of 50, 100, 200???M SNP significantly decreased the transport of Cd from roots to shoots, alleviated the inhibition of K, Ca, Mg and Fe, Cu, Zn absorption induced by Cd, reduced the toxicity symptoms and promoted the plant growth. The accumulation of reactive oxygen species (ROS) significantly increased in ryegrass seedlings exposed to Cd, and resulted in the lipid peroxidation, which was indicated by accumulated concentration of thiobarbituric acid-reactive substances. Addition of 50, 100, 200???M SNP significantly decreased the level of ROS and lipid peroxidation. Activities of antioxidant enzymes also showed the same changes. Addition of 50, 100, 200???M SNP increased activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase in ryegrass seedlings exposed to Cd. Addition of 100???M SNP had the most significant alleviating effect against Cd toxicity while the addition of 400???M SNP had no significant effect with Cd treatment. 相似文献
16.
Cadmium accumulation, the relative content of different chemical forms of Cd, as well as the toxic effect of Cd on nutrient
element uptake, physiological parameters, and ultrastructure of Sagittaria sagittifolia L. seedlings were determined after the seedlings were exposed to different Cd concentrations for 4 days. The results showed
that S. sagittifolia had the ability to accumulate large amounts of Cd. In the root, stem, and bulb, the predominant chemical Cd forms were NaCl
extractable. With an increase in the Cd2+ concentration, the chlorophyll content, the relative membrane penetrability (RMP) of root cells, peroxidase (POD) activity,
superoxide dismutase (SOD) activity in leaves, malondiadehyde (MDA) content and the superoxide anion (O2−) generation rate in roots all decreased following an initial increase. On the other hand, catalase (CAT) activity, SOD activity
in roots, MDA content, and the generation rate of O2− in leaves all increased gradually. The toxic effect of Cd2+ was more severe on roots than on leaves at the same concentration. Cadmium affected the mineral nutrition balance; mainly,
it promoted the uptake of Ca, Cu, Mn, and Fe, while inhibited Mg, Na, and K uptake. The physiological toxic effect of Cd2+ was close to the ultrastructural damage induced by Cd contamination. A significant correspondence was observed between the
Cd dose and its toxic effect. Cadmium could destroy the normal ultrastructure, disturb the ion balance, and interfere with
cell metabolism. 相似文献
17.
Nickel-induced Inhibition of Wheat Root Growth is Related to H2O2 Production, but not to Lipid Peroxidation 总被引:1,自引:1,他引:0
Effects of exogenous nickel (Ni: 10 and 200 μM) on growth, mitotic activity, Ni accumulation, H2O2 content and lipid peroxidation as well as the activities of various antioxidative enzymes, such as superoxide dismutase (SOD),
catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GSH-Px) were investigated in wheat roots. A considerable
Ni accumulation in the roots occurred at both the concentrations. Although Ni at 10 μM did not have any significant effect
on root growth, it strongly inhibited the root growth at 200 μM. Mitotic activity in the root tips was not significantly affected
by exposure of the seedlings to 10 μM Ni; however, it was almost completely inhibited at 200 μM treatment. Ni stress did not
result in any significant changes in CAT and APX activities as well as lipid peroxidation. However, H2O2 concentration increased up to 82% over the control in the roots of seedlings exposed to 200 μM Ni. There was a significant
decline in both SOD (50%) and GSH-Px (20–30%) activities in the roots when the seedlings were treated with 200 μM Ni. The
results indicated that a strong inhibition of wheat root growth caused by Ni stress was not due to enhanced lipid peroxidation,
but might be related to the accumulation of H2O2 in root tissue. 相似文献
18.
To investigate the antioxidative response of glutathione metabolism in Urtica dioica L. to a cadmium induced oxidative stress, activities of glutathione reductase (GR), glutathione-S-transferase (GST), and glutathione peroxidase (GSH-Px), content of reduced (GSH) and oxidized (GSSG) glutathione, lipid peroxidation (LPO), and also accumulation of Fe, Zn, Mn, Cu besides Cd were determined in the roots, stems, and leaves of plants exposed to 0 (control), 0.045, and 0.09 mM CdCl2 for 58 h. Whereas the Cd content continuously increased in all organs, the Fe, Zn, Mn, and Cu content decreased in dependence on the applied Cd concentration and incubation time. The Cd treatment resulted in increased GR and GST activities in all organs, however, GSH-Px activity was dependent on Cd concentration and plant organ. The GSH/GSSG ratio maintained above the control level in the stems at both Cd concentrations. The LPO was generally close to the control values in the roots and stems but it increased in the leaves especially at 0.09 mM Cd. 相似文献
19.
Posters Part 2 总被引:3,自引:0,他引:3
Elloumi Nada Ben Abdallah Ferjani Rhouma Ali Ben Rwina Bechir Mezghani Imed Boukhris Makki 《Acta Physiologiae Plantarum》2007,29(1):57-62
The effects of different CdCl2 concentrations on the growth and on certain biochemical parameters of almond seedlings (Prunus dulcis) were studied under controlled conditions in the nutrient solutions containing increasing CdCl2 concentrations ranging from 0 to 150 μM CdCl2. Under Cd stress conditions, damage was variable. Cadmium reduced dry matter production in leaves and roots. While chlorophyll
content was severely decreased, that of leaf sugars appeared to be increased. Furthermore, leaf nutritional status seemed
to be more altered than that of roots. Both in roots and leaves, there was an increase in MDA content as metal concentration
increased. It may be suggested from the present study that toxic concentrations of Cd cause oxidative damage as shown by the
increase of lipid peroxidation. 相似文献
20.
Fangming Yu Kehui Liu Mingshun Li Zhenming Zhou Hua Deng Bin Chen 《International journal of phytoremediation》2013,15(6):513-521
The effects of 60-d cadmium (Cd) exposure on enzymatic and non-enzymatic antioxidative system of Oryza sativa L. seedlings at tillering stage were studied using soil culture experiment. Research findings showed that chlorophyll content of Oryza sativa L. declined with the increase in soil metal concentration. Cd pollution induced the antioxidant stress by inducing O2 ?1 and H2O2, which increased in plants; at the same time, MDA as the final product of peroxidation of membrane lipids, accumulated in plant. The antioxidant enzyme system was initiated under the Cd exposure, i.e. almost all the activities of superoxide dismutase (SOD), peroxidase, catalase, glutathione peroxidase, and ascorbate peroxidase were elevated both in leaves and roots. The non-protein thiols including phytochelatins and glutathione to scavenge toxic free radicals caused by Cd stress was also studied. The contents of phytochelatins and glutathione were about 3.12–6.65-fold and 3.27–10.73-fold in leaves, against control; and the corresponding values were about 3.53–9.37-fold and 1.41–5.11-fold in roots, accordingly. 相似文献