New data on the reproductive biology of shortspine thornyhead <Emphasis Type="Italic">Sebastolobus alascanus</Emphasis> (Sebastidae) in the northwestern Pacific

New data on fecundity, sex ratio in different size groups, and the rate of sexual maturation of shortspine thornyhead Sebastolobus alascanus in the northwestern part of the Pacific Ocean are provided. Individual absolute fecundity of the studied females varies in the range of 175 000–821000 (on average, 392500) eggs. In Pacific waters off the Kuril Islands and Kamchatka, among individuals with a length to 41 cm, males dominate; in size groups of 42–59 cm, sex ratio is almost equal; among larger fish, females prevail. In the western part of the Bering Sea among analyzed fish with a length to 44 cm, sex ratio is almost equal; among larger individuals, females dominate. Half of males and females of shortspine thornyhead off the eastern coast of the northern Kuril Islands and southeastern Kamchatka mature at a length of approximately 41 cm that can be recommended as a commercial size for the given species in this area.     

Some reproductive characteristics of endangered Caspian Lamprey (<Emphasis Type="Italic">Caspiomyzon wagneri</Emphasis> Kessler, 1870) in the Shirud River southern Caspian Sea,Iran

Migration and reproduction of the Caspian Lamprey, Caspiomyzon wagneri, in the Shirud River were investigated during late-March to early-May at water temperatures ranging from 11 to 21.25°C. We examined the effect of water temperature on timing of spawning migrations. There was a significant negative relationship between temperature and intensive migration of Caspian Lamprey (p < 0.05). The most intensive migration of lampreys was at night (21:00–3:00 h) and when the water temperatures averaged 16°C (34.43%). The overall sex ratio (male to female) was 1.07 to 1. The individual absolute fecundity was 31 ‘758–51’ 198 eggs (mean±SD—41,924 ± 5,382). The egg diameter was 0.780–1.151 (0.92 ± 0.081) mm. The individual relative fecundity varies from 80.3 to 148.1 (107.2 ± 15.1) eggs per 1 mm of length and from 260.8 to 677.4 (397.6 ± 93) eggs per 1 g of weight. The gonadosomatic index (GSI) of females was 5.83–31.44 (11.22 ± 4.30).     

The reproductive cycle,size at maturity and fecundity of garfish (<Emphasis Type="Italic">Belone belone</Emphasis>, L. 1761) in the eastern Adriatic Sea

A recent study concerning the reproductive biology of the garfish (Belone belone, L. 1761) has been carried out in the eastern part of the Adriatic Sea along the Croatian coastline. Specimens of the fish (N = 3,393) were collected over a 6-year period (2003–2008). Their length varied between 20.8 and 75.4 cm (mean ± SD = 38.3 ± 7.94). Female garfish were dominant in larger length groups being most apparent during the resting phase of sexual cycle and in the peak of the spawning period, occurring in April and May. The sexual ratio of all specimens was m/f = 0.98. Males were prevalent in March—at the beginning of the highest spawning activities. Fifty percentage of the garfish population sexually matured at 28.5 cm of total length. Males and females reached their sexual maturity at 28.0 and 31.5 cm of total length, respectively. Spawning began in January peaking during March to May. According to their maturity stages, gonad weight and the gonadosomatic index, males began to spawn one month earlier (April) than females (May). The mean batch fecundity of garfish was 1,242.46 ± 843.64 of matured oocytes per ovary. Matured oocyte diameters ranged from 1.223 to 4.283 mm with the mean value of 2.269 ± 0.332 mm.     

Spawning population characteristics of pike <Emphasis Type="Italic">Esox lucius</Emphasis> L. in Lake Rubikiai (Lithuania)

The aim of the study was to describe the peculiarities of pike spawning in mesotrophic Lake Rubikiai, to determine the spawning population structure and evaluate the influence of some environmental factors on year-class strength formation. The data were collected in April and May (1994–2011). A total of 1586 individuals were caught. The age of pike ranged from 1 to 12 years; 2–5-yearold males (96.0%) and 3–8-year-old females (89.1%) prevailed. The overall sex ratio of females to males was 1:4.1. One-year-old spawning males (26.5–28.5 cm) and two-year-old spawning females (31.5–35.5 cm) constituted 2.1% and 2.6%, respectively. Water temperature during the spawning period was relatively stable, between 4 and 6°C, during March and increased slightly towards 10°C in the middle of April. No correlation was observed between female length and spawning date. Year-class strength did not correlate with the last day of ice presence and the minimal water level at the end of spawning (WL_min), but statistically significantly negatively correlated with the maximum water level at the beginning of spawning (WL_max) and the difference between WL_max and WL_min. The mean annual survival rate for pike (aged ≥2) was 0.74 and the mean annual instantaneous mortality rate was 0.45.     

Some issues related to catches of northern Atka mackerel <Emphasis Type="Italic">Pleurogrammus monopterygius</Emphasis> (Scorpaeniformes: Hexagrammidae) in the near-surface water layer of the northern Kuril Islands

Studies performed in Pacific waters of the northern Kuril Islands from May to August 2003–2007 demonstrated that not only juveniles of Pleurogrammus monopterygius but also its adult specimens are found in the near-surface layer. Some adult fish migrate to the near-surface layer for feeding, while occurrence in this layer for other specimens is related to the change of spawning grounds. Among specimens with a length of 36–48 cm, females comprise 86.2%; among specimens with a length of 22–28 cm, females comprise 56%. Migrations in the near-surface layer have no mass pattern.     

Aspects of the reproductive biology of two archer fishes <Emphasis Type="Italic">Toxotes chatareus</Emphasis>, (Hamilton 1822) and <Emphasis Type="Italic">Toxotes jaculatrix</Emphasis> (Pallas 1767)

Various aspects of the reproductive biology of two archer fishes Toxotes chatareus and Toxotes jaculatrix were studied to describe gonad development, spawning season, sex ratio, and fecundity. Spawning season was assessed using monthly changes in gonadosomatic index (GSI) and histological inspection of the gonads. Both species exhibit two modes of oocytes; a mode of small primary growth oocytes and a single mode that increases with size as oocytes sequester vitellogenin and undergo maturation, showing the synchronous oocyte development typical of total spawners. Based on GSI values and advanced stages of oocyte maturity, T. chatareus and T. jaculatrix, females appear to spawn from November to December. The average fecundity of female T. chatareus was 55 000 ± 5538 eggs, and T. jaculatrix was 50 000 ± 3440 eggs; fecundity ranged from 20 000 to 150 000 eggs for both species, with relative fecundities of 600 to 1100 eggs/g body weight, and a mean value of 800 ± 32 for T. chatareus; relative fecundity ranged from 500 to 1100 with a mean value of 700 ± 23 for T. jaculatrix. Sex ratio, defined as the proportion of males to females, was 2.2 and 2.5 in T. chatareus and T. jaculatrix, respectively. The apparent abundance of males in samples could be due to females being positioned lower in the water column and therefore being sampled less frequently. Our results indicate that in both species, spawning occurs between the months of November and December during the monsoon season, which provides the mangrove coastal waters inhabited by these species with an abundance of food resources and additional floodplain nursery habitat for larvae and juveniles.     

Batch fecundity and spawning frequency of invasive Hemiculter leucisculus (Basilewsky, 1855) in Erhai Lake,China

Hemiculter leucisculus are multiple spawners with indeterminate fecundity, although previous fecundity estimates have assumed a determinate spawning pattern. Batch fecundity and spawning frequency of H. leucisculus were studied in Erhai Lake, China, in which the fish, as an exotic species, has become a successful colonizer. Spawning frequency was 16.05 %, as determined from the percent of females with postovulatory follicles 12 to 36 h old during the peak reproductive period (from May 27 to August 8). The average interval between spawning was 6.2 days and there were more than 16 total spawning batches during the peak reproductive period. The average batch fecundity (mean ± SD) was 11,934?±?5,921 hydrated oocytes in 40 females (standard length or SL: 9.1–14.4 cm), while relative batch fecundity was 560?±?137 eggs g^?1 wet weight. The total potential annual fecundity was approximately 190,944 oocytes over the whole spawning season and was much higher than the estimated standing stock (31,585 oocytes) of yolked oocytes (36 females, SL: 9.5–16.2 cm) at the beginning of the spawning season in April 2010. This high annual fecundity is likely to have contributed to successful invasion of Erhai Lake by H. leucisculus.     

Reproductive biology of female striped marlin Kajikia audax in the western Pacific Ocean

Length and mass data for 1260 (536 females, 683 males, 41 sex unknown) striped marlin Kajikia audax were collected at the fish markets of Tungkang, Singkang and Nanfangao from July 2004 to September 2010. Of these samples, 534 gonads (236 females and 298 males) ranging from 95 to 206 cm in eye‐to‐fork length (L _EF ) and 8 to 88 kg in round mass (M _R), were collected. Chi‐square tests indicated sex ratios were homogeneous among months in 2004 and 2006–2008, but not in 2005, 2009 and 2010; and there were significant differences in sex ratio by size. The overall sex ratio (R _S ) differed significantly from the expected 0·5. Kajikia audax are sexually dimorphic and the proportions of females increased with size between 140 and 210 cm L _EF . Reproductive activity was assessed using a gonado‐somatic index (I _G ), external appearance of the gonads and histological examination and results indicated that the spawning season occurred from April to August with a peak in June to July. Based on histological observations and the distribution of oocyte diameters, K. audax are multiple spawners and their oocytes develop asynchronously. The estimated length‐at‐50% maturity (L _EF50 ) was c . 181 cm (c . 4·8 years of age) for females. The proportion of reproductively active females in the spawning season with ovaries containing postovulatory follicles (0·27) indicated that they spawned every 3·7 days on average. The hydrated oocyte method estimated mean ± S.D. batch fecundity (F _B ) to be 4·4 ± 2·02 million eggs; average relative fecundity was 53·6 ± 13·9 oocytes g^?1 M _R; and the average annual fecundity was 181·3 ± 48·3 million eggs. The parameters estimated in this study are key information for stock assessments of K. audax in the north‐western and central Pacific and will contribute to the conservation, management and sustainable yield of this species.     

Age composition,growth, and reproductive biology of yellow catfish (<Emphasis Type="Italic">Peltobagrus fulvidraco</Emphasis>, Bagridae) in Ce Lake of Hubei Province,Central China

This study presents fundamental information on the reproductive biology, size and age composition, and growth of yellow catfish, Peltobagrus fulvidraco. Sampling was carried out monthly from Ce Lake in Hubei province from January 2004 and December 2004. Overall female/male (F/M) sex ratio was 1.08, not significantly different from expected 1.0 ratio. But sex ratio (F/M) of the spawning group was 1.14:1 with skewness towards females (p < 0.05). The smallest sexually mature male and female massed 20.51 and 16.93 g, and measured 100.1 and 94.3 mm in total length, respectively. Reproductive period for this species is from April to August when GSI is considerably higher than the rest months (p < 0.05). The highest GSI values were measured from May to July for both sexes and this period was presumably the peak spawn. First maturation occurred at age 1⁺ for both sexes. Fish of age 1⁺ and 2⁺ comprised the greatest portion of spawners. The absolute fecundity (AF) of P. fulvidraco ranged from 657 to 11,696 eggs per fish, with mean AF value of 3,570 ± 122 (S.D.) eggs per fish, varying considerably at given length, weight and age. The fecundity relative to total length (F/L) fluctuated from 58 to 680 eggs/mm, with a mean value of 246 ± 34 (SD) eggs/mm, and fecundity relative to total weight (F/W) varied from 29–133 eggs/g, with a mean value of 67 ± 7 (SD) eggs/g. P. fulvidraco is a single spawner, with low fecundity compared with other Bagrid fish.     

Reproductive biology of albacore Thunnus alalunga

Reproductive variables in albacore Thunnus alalunga were evaluated by gonad histology in samples of 132 males (58–118 cm fork length, L_F) and 112 females (59–101 cm L_F) that were collected from the western North Pacific Ocean from 2001 to 2006. In the sex ratio examination, males greatly outnumbered females in large adult fish (L_F > 100 cm). Thunnus alalunga exhibited a protracted spawning period from March to September in the waters off eastern Taiwan and the Philippines, and the peak spawning activity occurred in March and April. Minimum sizes associated with the classification of mature fish were 78 and 83 cm L_F for males and females, respectively. In addition, the largest L_F of immature fish were 93 cm for males and 94 cm for females. The spawning frequency estimate in April was 1·7 days. Batch‐fecundity estimates of 21 females (89–99 cm L_F) ranged between 0·17 and 1·66 million eggs (mean ±s.d . = 0·94 ± 0·43). The relative fecundity estimates of the 21 females ranged between 9·2 and 92·4 oocytes g^?1 body mass (mean ±s.d . = 50·5 ± 22·8). The results presented in this study provide increased information regarding this species' reproductive‐related characteristics than are currently available in stock status determinations.     

Fecundity of the American eel Anguilla rostrata at 45° N in Maine, U.S.A.

The northern portion of the geographic range of the American eel Anguilla rostrata may contribute a great proportion of the reproductive potential to this panmictic species because of apparent increases in average female size and female percentage with latitude. The regressions of fecundity on body length and on body weight of 63 female eels captured at about 45° N latitude on their spawning migration to the sea were log F= 1·2601 + 2·9642 log L and log F= 4·1646+0·9153 log W , where F is fecundity, L is total length (cm), and W is total weight (g). Length and weight each explained about 90% of the variation in fecundity. Estimates of fecundity from counts of aliquots of eggs ranged from 1·84 million to 19·92 million eggs for eels ranging in length from 45 to 113 cm, nearly the range of sizes of migrating females reported in the literature. Fecundities of the American eel were greater than reported in one study at about 37° N and greater than reported for the European eel, A. anguilla , shortfin eel, A. australis , and longfin eel, A. dieffenbachii . If a geographic cline in fecundity does exist in American eels, it is established anew each generation because the species forms a single panmictic population.     

The morphometrics and colouration of the European pond turtle Emys orbicularis in Lubuskie province (West Poland)

The European pond turtle Emys orbicularis, is an endangered species in Poland. The region of the Ilanka River, in the western part of the country (Lubuskie province), is the most densely inhabited area by the species. Several populous sites are dispersed over the whole area of the province. Over the past 10 years, 279 turtles were captured at 11 sites. In 218 individuals, those with a straight carapace length (SCL) between 2.10–12.10 cm, sex was not determined; the remaining 61 with SCL 13.33–19.85 cm were sexed. Females had bigger bodies and were heavier than males. The SCL of females was 17.10 ± 1.7 cm (average ± SD, n = 33), their body mass (BM) was 790.6 ± 228.7 g (n = 27), and in the case of males SCL was 15.44 ± 1.5 cm and BM was 554.2 ± 138.8 g (n = 28). SCL of the smallest egg laying female was 15.30 cm, and SCL of the smallest mating male was 13.33 cm. The colouration of the turtles which undergoes change with age can be varied.     

Reproductive biology and spawning strategy of the catadromous percichthyid, <Emphasis Type="Italic">Macquaria colonorum</Emphasis> (Günther, 1863)

The reproductive biology and spawning strategy of the iconic catadromous percichthyid, estuary perch Macquaria colonorum was examined from three coastal rivers of south-eastern (SE) Australia. Ovarian and testicular development followed a pattern similar to that of other percichthyids, world-wide. Females were collected in significantly larger sizes and greater numbers from all, and two of the three rivers, respectively. Mean size and age at maturity was greater for females (25.05 cm FL ± 0.85 and 3.75 yrs ± 0.08, respectively) than males (22.21 cm FL ± 0.50 and 3.28 yrs ± 0.08 respectively), with no significant difference between rivers. Trends in gonadosomatic indices indicated that M. colonorum spawn in the austral winter-spring in SE Australia. However, peak spawning occurred earlier (June-August) in the two most northern rivers (Hawkesbury and Clyde) compared with the Bemm River (September-November) further south. Ripe females were captured only in the lower reaches of estuaries throughout the spawning period in all three rivers. Mature M. colonorum females displayed a group synchronous pattern of oocyte development suggesting a multiple spawning strategy. Mean batch fecundity (F _B ) was 266 583 ± 17 821 oocytes per female and relative fecundity was 377 ± 10 oocytes.g^-1 body weight. There were significant power relationships between F _B and fork length (FL), weight and age. Similar to other percichthyids, M. colonorum are long-lived, highly fecund and exhibit a flexible spawning strategy which is attuned to the highly variable environments in which they are found.     

Biology ofMicroplitis mediator [Hym.: Braconidae] a gregarious endoparasite ofAgrotis segetum [Lep.: Noctuidae]

Microplitis mediator (Haliday) a gregarious endoparasite was recorded for the first time fromAgrotis segetum (Schiff) in Ankara, Turkey. The female parasites found their hosts by responding to the faeces of the caterpillars. An average, females laid 15.5±1.6 eggs in the bodies of their hosts. The newly laid eggs were elongated, oval in shape and 0.23±0.004 mm long and 0.07 mm wide. They hatched in 5, 4 and 3 days at 20±2°C, 25±2°C and 30±2°C respectively when maintained at 60–70% R.H. and 14∶10 light∶dark regime. At the same temperatures, the larval stage lasted for 24.9±0.6, 18.2±0.4 and 17.1±0.5 days respectively. The prepupal stage was completed in 2 days at 25±2°C, whereas the prepupal and pupal (cocoon) stage lasted 10.9±0.2, 7.0±0.1 and 6.2±0.1 days respectively at the temperatures mentioned above. The adults started mating and feeding shortly after emergence. Female parasites started laying after one day, 7–11 hours and 5–7 hours at the temperatures stated above. At these temperatures females lived for 10.8±0.2, 5.4±0.1, 4.6±0.1 days and laid on average 556, 484 and 363 eggs respectively, whereas the males survived 10.5±0.3, 4.7±0.1 and 4.4±0.1 days respectively.      

Effect of female aging on the morphology and hatchability of resting eggs in the rotifer <Emphasis Type="Italic">Brachionus plicatilis</Emphasis> Müller

This study examined the morphology and hatchability of Brachionus plicatilis resting eggs as a function of the aging of maternal fertilized mictic females. One-hundred twenty fertilized B. plicatilis (Australian strain) were individually cultured and monitored daily until death. All cultures were maintained at 25°C, 11 ppt, and fed the micro-algae Tetraselmis tetrathele. Resting eggs produced by the females were investigated using two parameters: egg morphology, and hatching rate. Under these culture conditions, females normally produce 1–6 (mean ± SD = 2.7 ± 1.2) resting eggs during their lifetime. However, the number of resting eggs with abnormal morphology increased as a function of maternal age. Among resting eggs with normal morphology (n = 225), 82.2% were produced during the first and second spawning, and had hatching rates of more than 60%, while the hatching rates were below 30% in resting eggs with a spawning order of >2. Thus, the quality of B. plicatilis resting eggs was negatively correlated with maternal age.     

Predation of Northern Pike (Esox lucius L.) Eggs: A Possible Cause of Regionally Poor Recruitment in the Baltic Sea

Northern pike (Esox lucius) spawning habitat and egg mortality were studied in three spawning areas in 2001 along the Swedish coast of the Baltic proper: the Blekinge Archipelago, Kalmar Sound and coastal freshwater streams. Spawning peaked during the last week of April in streams, at temperatures ranging from 7.7 to 8.9 °C and during the first week of May in brackish waters, at temperatures ranging from 8.9 to 13.8 °C. Spawning occurred in shallow waters, at depths between 0.2–1.5 m, but generally most of the spawn was found in the shallowest areas. In streams, eggs were mainly attached to emersed vegetation, while in brackish sites pike eggs were well scattered among flooded emersed plants, submersed plants and filamentous algae. Mean egg density varied between 469–1829 m^–2 with the lowest density observed in Kalmar Sound. The calculated egg loss occurring from approximately one day after spawning to one day before hatching ranged from 41±7% in coastal streams to 67±6% in the Blekinge Archipelago and 100% in Kalmar Sound. The significant removal of eggs from spawning sites in Kalmar Sound and Blekinge was most likely due to predation from several fish species. In situ observations and stomach analyses suggested that many pike eggs in Kalmar Sound were lost to the three-spined stickleback (Gasterosteus aculeatus), a species that presently dominate the littoral small fish community. This study therefore suggests that egg predation by sticklebacks and other fishes may be a possible cause of the reported poor recruitment of coastal pike populations in the Kalmar Sound region.     

Length–weight and length–length relationships of the bluefin tuna,Thunnus thynnus L., in the Turkish part of the eastern Mediterranean Sea

This study describes length–weight (LWR) and length–length relationships (LLR) of bluefin tuna (BFT), Thunnus thynnus, in the eastern Mediterranean (Levantine Sea). Fulton’s condition factor (K) was also calculated for each standard length class for both sexes. A total of 363 specimens, 175 males and 188 females, were caught by purse‐seine from late winter to early summer 2003 off Antalya Bay, Turkey. Fork length values of 175 BFT males were between 114 and 250 cm with an average of 159.3 cm ± 2.14, and between 116 and 256 cm with an average of 163 cm ± 2.26 in 188 females. LWRs indicated positive allometric growth in both males and females. The coefficient b ranged from a minimum 3.32 for males to a maximum of 3.35 for females. Results indicated that the LLRs were highly correlated (r² > 0.99, P < 0.001). K values ranged from 1.45 to 2.11 (mean: 1.56 ± 0.12) in males and from 1.43 to 2.14 (mean: 1.69 ± 0.06) in females.     

Biology and host specificity of <Emphasis Type="Italic">Gonatocerus deleoni</Emphasis> (Hymenoptera: Mymaridae), a potential biocontrol agent of <Emphasis Type="Italic">Homalodisca vitripennis</Emphasis> (Hemiptera: Cicadellidae) in California,USA

The host-specificity and biological traits of Gonatocerus deleoni Triapitsyn, Logarzo & Virla (Hymenoptera: Mymaridae), a potential candidate for biological control of the glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), were determined under laboratory conditions. G. deleoni is a solitary egg parasitoid native to Argentina, originally reared from sentinel eggs of Tapajosa rubromarginata (Signoret) (Cicadellidae). With GWSS as a fictitious host, G. deleoni’s average development time from oviposition to adult emergence was 18.8 ± 1.4 days, with males developing faster than females (18.0 ± 1.3 days, males; 19.0 ± 1.3 days, females). The average parasitism rate on 1–8-day-old eggs was 45.7% but this was significantly affected by the age of the egg, ranging from 1.4% to 69.9% (egg ages 8 and 3, respectively). The average sex ratio was 0.34 (percent males) and sex ratio was not significantly affected by egg age. G. deleoni was able to develop in eggs of GWSS and Homalodisca liturata Ball (both in the tribe Proconiini), but was unable to develop on eggs of Graphocephala atropunctata (Signoret) (different tribe, same subfamily) or Erythroneura elegantula Osborn (different subfamily).     

Seasonal and interannual variation of the ichthyoplankton off the coast of Northern Morocco

Changes in the qualitative and quantitative composition of the ichthyoplankton off the coast of Northern Morocco (36–28° N) are analyzed in different seasons of 1994–2008. Pelagic eggs and larvae of over 80 fish species belonging to 57 families are recorded. In cold periods at the Morocco coast, there is maximum spawning of fish belonging to the subtropical (Mediterranean-Atlantic) fauna, the dominant of which are European pilchard Sardina pilchardus, common scad Trachurus trachurus, and Atlantic mackerel Scomber japonicus. In warm periods, in addition to the aforementioned species, the ichthyopankton often comprises eggs and larvae of fish form the tropical fauna. Among the latter are round sardinella Sardinella aurita and West-African mackerel T. trecae dominate. The spawning biomass of principal commercial fish is also calculated by the quantity of eggs laid by females.     

Saline acclimation of striped mojarra <Emphasis Type="Italic">Eugerres plumieri</Emphasis> (Cuvier 1830) and optimal dosage of carp pituitary extract (CPE) to induce spawning

The main objective of the current study was to determine an optimal dosage of commercial carp pituitary extract (CPE) of the conventional heteroplastic hypophysation technique to induce spawning in the wild caught striped mojarra broodstock Eugerres plumieri under laboratory conditions. We also describe trials testing saline acclimation regimes (changes from 10‰ to 30‰) prior to hormonal induction. For saline acclimation, three treatments were performed: first and second treatments began 1-day after conditioning of broodstock fish with a total duration of the saline changes lasting 1-day and 2-days, respectively. The third treatment began 30-days after conditioning with a 7-day saline regime. After reaching 30‰, all fish from the first and second treatments died after the fourth and ninth days, respectively; while the fishes in the third treatment survived more than two years. These fish remain on public exhibit in the “Mundo Marino” Aquarium, Santa Marta, Colombia. Four treatments of hormonal induction were tested on females using a total concentration of 5-mg of CPE per kg of body weight: first, second and third treatments were applied in two hormonal doses that corresponded to 10%–90%, 30%–70% and 40%–60%, respectively, with time intervals between doses of 14-h for the first and second treatment and of 12-h for the third treatment. The fourth treatment was applied in four hormonal doses that it corresponded to 20%–20%–30%–30% with 12-h time intervals between doses. Only the fishes of the fourth treatment resisted the induction, continued to live and reached spawning artificially. Water temperature remained at 28 ± 1°C and at 30‰ salinity during the study. The “dry” method of fertilization was used. The fertilized eggs were incubated at 30‰ and 35‰ salinities. These eggs reached the Morula early stage, but were later attacked by protozoan. These results suggest that fast changes of saline and limited hormonal dosages do not offer effective results in Eugerres plumieri. This work provided fundamental procedures for the culture and maintenance of live broodstock for striped mojarra in saltwater and/or coastal lagoon habitats and provides an effective and viable dosage of CPE for artificial spawning and commercial production in this species.