Comparative Evidence that Salt Marshes and Mangroves May Protect Seagrass Meadows from Land-derived Nitrogen Loads

Seagrass meadows within estuaries are highly sensitive to increased supplies of nitrogen (N). The urbanization of coastal watersheds increases the delivery of N to estuaries, threatening seagrass habitats; both seagrass production per unit area and the area of seagrass meadows diminish as land-derived N loads increase. The damaging effects of land-derived N loads may be lessened where there are fringes of coastal wetlands interposed between land and seagrass meadows. Data compiled from the literature showed that production per unit area by seagrasses increased and losses of seagrass habitat were lower in estuaries with relatively larger areas of fringing wetlands. Denitrification and the burial of land-derived N within fringe wetlands may be sufficient to protect N-sensitive seagrass habitats from the detrimental effects of land-derived N. The protection furnished by fringing wetlands may be overwhelmed by increases in anthropogenic N loads in excess of 20–100 kg N ha⁻¹ y⁻¹. The relationships of land-derived N loadings, fringing coastal wetlands, and seagrass meadows demonstrate that different units of the landscape mosaic found in coastal zones do not exist as separate units, but instead are coupled and uncoupled by biogeochemical transformations and transport among environments. Received 12 December 2000; accepted 15 August 2001.     

Interstitial Protozoa and Algae of Louisiana Salt Marshes

SYNOPSIS. The majority of Louisiana salt marshes consists of stagnant pools among a vegetation of Spartina spp. and Distichlis spicata. The sediments constitute a "sulphide biome,'with Eh values reaching to –350 mV. The 200 interstitial ciliate species had definite stratification. Generally, algivores and omnivores occurred in the upper, and bactivores in the lower layers of the sediments. Compaction of particles prevented the flexible and large ciliates from inhabiting the lower layers, while Eh below –200 mV limited the distribution of some species but favored certain bactivores. The pH/Eh range and the abundance of nutrients in the sulfide biome provide habitable conditions for all the major groups of microflora. These, in turn, support nutritionally diverse predatory ciliate populations.     

Trophic Dynamics and Niches of Salt Marsh Foraminifera

Energetic considerations of the growth of three species of littoralbenthic foraminifera, Allogromia laticollaris, Rosalina leei,and Spiroloculina hyalina, have been made on laboratory-grownpopulations. Under optimum laboratory conditions A. laticollarishas the greatest intrinsic rate of increase (r = 2.533 org/day);S. hyalina (r = 1.472 org/day), and R. leei (r = 0.272 org/day)being less fecund. The respiration rates of the three specieswere similar (0.5–4.5 µ1/mg body wt/hr) within thetemperature range (15–35 C) tested. The species studiedare selective feeders. Only 4-5 of 28 species of algae testedwere consumed in significant quantities (40-150 x 10⁸ g/foram/day).Although great numbers of bacteria were eaten, their biomasswas negligible when compared to the algae. The ecological growthefficiency (E_e) of the three species tested is highest in freshcultures (5-20%) and declines rapidly. Evidence suggests thatthe species studied are well adapted for the rapid changes inthe microbial community structure which take place throughoutthe summer, and that community stability and high rate of productivityare achieved through diversity.     

中国盐沼湿地蓝碳碳汇研究进展

滨海蓝碳主要指被红树林、盐沼湿地、海草床等蓝碳生态系统所固定的碳,这部分碳对于减缓气候变暖意义重大。其中盐沼湿地作为我国面积最大、分布广泛的滨海湿地,受到人类活动的扰动较多,其碳汇估算的数据缺乏系统性与完整性。通过收集我国的盐沼湿地相关研究与数据,本文对我国盐沼湿地的分布现状及其碳储量、碳埋藏、碳来源、温室气体通量进行了总结,其中我国盐沼湿地的分布面积为(1.27~3.43)×10⁵hm²,总碳储量为(7.5±0.6) Tg,碳埋藏速率为7~955 g C/(m²·a),非CO₂温室气体通量分别为23.6~986 μg CH₄/(m²·h)和1.58~110 μg N₂O/(m²·h)。本文系统梳理了有关我国盐沼湿地碳汇功能的研究,指出我国盐沼湿地碳循环研究仍需加深对机制机理的解析和关键调控因子的探究,以期让盐沼湿地蓝碳为我国的碳达峰与碳中和战略做出更大贡献。     

Relationships between Sediment Microbial Communities and Pollutants in Two California Salt Marshes

Salt marshes are important ecosystems whose plant and microbial communities can alter terrestrially derived pollutants prior to coastal water discharge. However, knowledge regarding relationships between anthropogenic pollutant levels and salt marsh microbial communities is limited, and salt marshes on the West Coast of the United States are rarely examined. In this study, we investigated the relationships between microbial community composition and 24 pollutants (20 metals and 4 organics) in two California salt marshes. Multivariate ordination techniques were used to assess how bacterial community composition, as determined by terminal restriction fragment length polymorphism and phospholipid fatty acid analyses, was related to pollution. Sea urchin embryo toxicity measurements and plant tissue metabolite profiles were considered two other biometrics of pollution. Spatial effects were strongly manifested across marshes and across channel elevations within marshes. Utilizing partial canonical correspondence analysis, an ordination technique new to microbial ecology, we found that several metals were strongly associated with microbial community composition after accounting for spatial effects. The major patterns in plant metabolite profiles were consistent with patterns across microbial community profiles, but sea urchin embryo assays, which are commonly used to evaluate ecological toxicity, had no identifiable relationships with pollution. Whereas salt marshes are generally dynamic and complex habitats, microbial communities in these marshes appear to be relatively sensitive indicators of toxic pollutants.     

Outline of A New Approach to Evaluate Ecological Integrity of Salt Marshes

The integrity of coastal salt marshes can be determined from the extent to which they provide key ecosystem services: food and habitat for fish and wildlife, good water quality, erosion and flood control, and recreation and cultural use. An outline of a new approach for linking ecosystem services with metrics of structure and function to evaluate the ecological integrity of salt marshes is described. One main objective of the approach is to determine whether differences in structure and function can be detected among salt marshes with similar geomorphology and hydrology but different degrees of anthropogenic stress. The approach is currently being applied to salt marshes of Narragansett Bay, RI, USA. Stable nitrogen isotopic ratios of the marsh biota reflected the nitrogen sources from the adjacent watersheds and were significantly correlated with percent residential land use. Results show that plant zonation significantly ( r = —0.82; p < 0.05) relates with percent residential land use and is potentially a sensitive indicator of anthropogenic disturbance of New England salt marshes. We are currently examining species diversity, denitrification rates, and susceptibility to erosion among the sites for additional indicators of salt marsh condition. Our results to date suggest that this approach will provide the methods needed for managers to systematically monitor and evaluate the integrity of salt marshes     

Outline of A New Approach to Evaluate Ecological Integrity of Salt Marshes

The integrity of coastal salt marshes can be determined from the extent to which they provide key ecosystem services: food and habitat for fish and wildlife, good water quality, erosion and flood control, and recreation and cultural use. An outline of a new approach for linking ecosystem services with metrics of structure and function to evaluate the ecological integrity of salt marshes is described. One main objective of the approach is to determine whether differences in structure and function can be detected among salt marshes with similar geomorphology and hydrology but different degrees of anthropogenic stress. The approach is currently being applied to salt marshes of Narragansett Bay, RI, USA. Stable nitrogen isotopic ratios of the marsh biota reflected the nitrogen sources from the adjacent watersheds and were significantly correlated with percent residential land use. Results show that plant zonation significantly ( r = —0.82; p < 0.05) relates with percent residential land use and is potentially a sensitive indicator of anthropogenic disturbance of New England salt marshes. We are currently examining species diversity, denitrification rates, and susceptibility to erosion among the sites for additional indicators of salt marsh condition. Our results to date suggest that this approach will provide the methods needed for managers to systematically monitor and evaluate the integrity of salt marshes     

Dynamics of Type IV Pili Is Controlled by Switching Between Multiple States

Type IV pili are major bacterial virulence factors supporting adhesion, surface motility, and gene transfer. The polymeric pilus fiber is a highly dynamic molecular machine that switches between elongation and retraction. We used laser tweezers to investigate the dynamics of individual pili of Neisseria gonorrheae at clamped forces between 8 pN and 100 pN and at varying concentration of the retraction ATPase PilT. The elongation probability of individual pili increased with increasing mechanical force. Directional switching occurred on two distinct timescales, and regular stepping was absent on a scale > 3 nm. We found that the retraction velocity is bimodal and that the bimodality depends on force and on the concentration of PilT proteins. We conclude that the pilus motor is a multistate system with at least one polymerization mode and two depolymerization modes with the dynamics fine-tuned by force and PilT concentration.     

The Role of Fundulus heteroclitus in Salt Marsh Trophic Dynamics

The common mummichog, Fundulus heteroclitus, functions as bothpredator and prey in the trophic structure of east coast tidalmarshes. Although mummichogs are generally considered importantto energy transformations within marshes, few studies have convincinglydemonstrated that predation by F. heteroclitus affects the abundanceof salt marsh benthic invertebrates. Thus far investigationsof this type have dealt only with the direct effects of adultmummichogs. The results of recent experiments have suggestedthat by controlling smaller predators, mummichogs may indirectlyhave a positive effect on the densities of some infaunal marshinvertebrates. Our current knowledge of larval and juvenilemummichogs in their natural habitat is minimal. Unlike the adults,which can utilize the intertidal zone only when it is flooded,the young remain on the marsh even at low tide, inhabiting shallowpuddles of residual tidal water that form between clumps ofvegetation and around fiddler crab (Uca sp.) burrows. The importanceof F. heteroclitus in salt marsh communities will remain incompletelyunderstood unless future studies consider the role of larvaland juvenile mummichogs. Although many species of fishes andwading birds feed on mummichogs, the blue crab (Callinectessapidus) is probably the major predator of adult F. heteroclitusin the intertidal salt marsh. Predation by adult mummichogsand xanthid crabs (e.g., Eurytium limosum) may contribute tothe high mortality of larval and juvenile Fundulus.     

Isolation of Bacteriophages of the Marine Bacterium Beneckea natriegens from Coastal Salt Marshes

Bacteriophages of the marine bacterium Beneckea natriegens were isolated from coastal marshes where they were limited to brackish and marine waters. The phages were widely distributed and morphologically diverse in the marshes.     

Tidal Flushing Restores the Physiological Condition of Fish Residing in Degraded Salt Marshes

Roads, bridges, and dikes constructed across salt marshes can restrict tidal flow, degrade habitat quality for nekton, and facilitate invasion by non-native plants including Phragmites australis. Introduced P. australis contributes to marsh accretion and eliminates marsh surface pools thereby adversely affecting fish by reducing access to intertidal habitats essential for feeding, reproduction, and refuge. Our study assessed the condition of resident fish populations (Fundulus heteroclitus) at four tidally restricted and four tidally restored marshes in New England invaded by P. australis relative to adjacent reference salt marshes. We used physiological and morphological indicators of fish condition, including proximate body composition (% lipid, % lean dry, % water), recent daily growth rate, age class distributions, parasite prevalence, female gravidity status, length-weight regressions, and a common morphological indicator (Fulton’s K) to assess impacts to fish health. We detected a significant increase in the quantity of parasites infecting fish in tidally restricted marshes but not in those where tidal flow was restored to reduce P. australis cover. Using fish length as a covariate, we found that unparasitized, non-gravid F. heteroclitus in tidally restricted marshes had significantly reduced lipid reserves and increased lean dry (structural) mass relative to fish residing in reference marshes. Fish in tidally restored marshes were equivalent across all metrics relative to those in reference marshes indicating that habitat quality was restored via increased tidal flushing. Reference marshes adjacent to tidally restored sites contained the highest abundance of young fish (ages 0–1) while tidally restricted marshes contained the lowest. Results indicate that F. heteroclitus residing in physically and hydrologically altered marshes are at a disadvantage relative to fish in reference marshes but the effects can be reversed through ecological restoration.     

Alternate Stable States and the Shape of the Lake Trophic Distribution

Ecological theory applied to small and shallow lakes suggests that water clarity and primary productivity may be bimodal, reflecting alternate stable states. This hypothesis was tested using remote sensing data. We used estimated Secchi disk transparency derived from reflected light measured by Landsat satellite photosensors. Lake trophic state indices (TSI) were estimated from the transparency estimates. Because alternate stable state theory is typically applied to productive lakes, we predicted that planktonic primary productivity would be especially bimodal in the small and shallow eutrophic lakes of southern Wisconsin. We found that overall, trophic state for over 8000 lakes in Wisconsin was multimodal (at least bimodal). Frequency distributions for lake size categories appeared to be distinctly bimodal. The largest and smallest lakes for all of Wisconsin had significantly bimodal curves, and southern (more productive) lakes had a more distinct multimodal curve than northern lakes. These results support the basic predictions of alternate stable state theory. The significantly trimodal distribution for southern lakes suggests that they may exist in more than just two alternate stable states.     

A Monitoring Protocol to Assess Tidal Restoration of Salt Marshes on Local and Regional Scales

Assessing the response of salt marshes to tidal restoration relies on comparisons of ecosystem attributes between restored and reference marshes. Although this approach provides an objective basis for judging project success, inferences can be constrained if the high variability of natural marshes masks differences in sampled attributes between restored and reference sites. Furthermore, such assessments are usually focused on a small number of restoration projects in a local area, limiting the ability to address questions regarding the effectiveness of restoration within a broad region. We developed a hierarchical approach to evaluate the performance of tidal restorations at local and regional scales throughout the Gulf of Maine. The cornerstone of the approach is a standard protocol for monitoring restored and reference salt marshes throughout the region. The monitoring protocol was developed by consensus among nearly 50 restoration scientists and practitioners. The protocol is based on a suite of core structural measures that can be applied to any tidal restoration project. The protocol also includes additional functional measures for application to specific projects. Consistent use of the standard protocol to monitor local projects will enable pooling information for regional assessments. Ultimately, it will be possible to establish a range of reference conditions characterizing natural tidal wetlands in the region and to compare performance curves between populations of restored and reference marshes for assessing regional restoration effectiveness.     

Physical Evolution of Restored Breached Levee Salt Marshes in the San Francisco Bay Estuary

Since 1972 over 940 ha (2,300 ac) of leveed former salt marsh sites around San Francisco Bay have been restored to tidal action, purposely or through natural processes. The evolution of these sites can inform predictions of rates of marshplain evolution and establishment of tidal channel systems. A review of the history of 15 re‐flooded sites ranging in size from 18 to 220 ha (45 to 550 ac) and in age from 2 to 29 years indicates that marshplain vegetation with more than 50% cover was established at nine of the sites within 4 to 20 years. The remaining six sites aged 2 to approximately 20 years continue to be less than 50% vegetated. The evolution of these sites is consistent with the following simple conceptual model of the physical evolution of restored tidal marshes in subsided breached sites. Initially, deposition of estuarine sediment builds up mudflats that allow vegetation establishment once elevations are high enough for vegetation to survive. Sites that are initially lower in the tidal frame take longer to vegetate than those that are initially higher. Three factors appear to retard the time frame for vegetation establishment: limited estuarine suspended sediment supply, erosion of deposited estuarine muds by internally generated wind waves, and restricted tidal exchange. These factors affect evolution more significantly in larger sites. The comparatively short time frame for vegetation colonization and marshplain evolution experienced in earlier, smaller, and/or less subsided breached levee restorations may not necessarily be replicable by simple levee breaching on larger subsided restoration sites now being planned. Our review of the 15 sites also indicates that the formation of tidal channels within the marshes is greatly dependent on whether and how high the site was filled before breaching. Filled sites at high intertidal elevations (above approximately 0.3 m below mean higher high water) can vegetate quickly but after several decades may show little development of tidal channels.     

Declining Diversity in Natural and Restored Salt Marshes: A 30-Year Study of Tijuana Estuary

Between 1974 and 2004, Tijuana Estuary's natural salt marsh underwent pulse disturbance (an 8-month nontidal period in 1984), which caused the sudden loss of two short-lived halophytes ( Salicornia bigelovii [Sb] and Suaeda esteroa [Se]) and rapid dominance of a productive native succulent ( Sa. virginica [Sv]), plus ramp disturbance that led to gradual codominance by Jaumea carnosa (Jc) (another productive succulent) by 1994. Species richness was high in 1974 (4.2 species/0.25-m² plot), low in 1984 (1.4 species), and not fully recovered by 1994 (3.7 species) or 2004 (3.9 species). Restoration efforts (reseeding former habitat and excavating and planting new sites) did not recover the populations of Sb or Se. In a 1997 project, plantings of these and six other native halophytes survived initially, but by 2005, short-lived species were lost and Sv and Jc dominated, as in the natural marsh. In a 2000 restoration site, planting mortality was high for five species, but Sv recruited voluntarily and dominated by 2005. We attribute recent vegetation changes to frequent catastrophic storms, flooding, and sedimentation, which contrasted strongly with the benign conditions of decades prior to 1974. Sediment blocked tidal channels in 1984 and gradually elevated the marsh plain, degrading the diverse salt marsh and hindering efforts to restore it. Future restoration efforts will require even greater control over sediment inflows plus contouring sites to include natural topographic features that appear critical to sustaining high species richness and evenness.     

Geographic Variation in Plant Community Structure of Salt Marshes: Species,Functional and Phylogenetic Perspectives

In general, community similarity is thought to decay with distance; however, this view may be complicated by the relative roles of different ecological processes at different geographical scales, and by the compositional perspective (e.g. species, functional group and phylogenetic lineage) used. Coastal salt marshes are widely distributed worldwide, but no studies have explicitly examined variation in salt marsh plant community composition across geographical scales, and from species, functional and phylogenetic perspectives. Based on studies in other ecosystems, we hypothesized that, in coastal salt marshes, community turnover would be more rapid at local versus larger geographical scales; and that community turnover patterns would diverge among compositional perspectives, with a greater distance decay at the species level than at the functional or phylogenetic levels. We tested these hypotheses in salt marshes of two regions: The southern Atlantic and Gulf Coasts of the United States. We examined the characteristics of plant community composition at each salt marsh site, how community similarity decayed with distance within individual salt marshes versus among sites in each region, and how community similarity differed among regions, using species, functional and phylogenetic perspectives. We found that results from the three compositional perspectives generally showed similar patterns: there was strong variation in community composition within individual salt marsh sites across elevation; in contrast, community similarity decayed with distance four to five orders of magnitude more slowly across sites within each region. Overall, community dissimilarity of salt marshes was lowest on the southern Atlantic Coast, intermediate on the Gulf Coast, and highest between the two regions. Our results indicated that local gradients are relatively more important than regional processes in structuring coastal salt marsh communities. Our results also suggested that in ecosystems with low species diversity, functional and phylogenetic approaches may not provide additional insight over a species-based approach.     

The Mineral Ion Relations of Mangroves I. Root Cell Compartments in a Salt Excluder and a Salt Secreter Species at Low Salinities

The efflux kinetics of ²²Na and ³⁶Cl from 48 h pre-loaded Rhizophoramangle and Avicennia germinans mangrove seedling roots grownin low salinity conditions have been documented. Estimates ofthe percent isotope distributed throughout the whole plant,the uptake rates, the amounts calculated to be present in theroot cell compartments, and the rate constants and half-timesof efflux were made. Little to no ²²Na or ³⁶Cl was noted asbeing distributed to leaf tissue in the red mangrove, Rhizophoramangle, substantiating its characterization as a non-secreting,salt excluding species. Significantly greater net uptake ratesfor Na and Cl were observed for the black mangrove, Avicenniagerminans, a non-excluding, salt-secreting species. The typicalintracellular compartmentation found in higher plant root tissueswas noted to be present in these mangroves and the efflux kineticsclearly indicated that Na and Cl were handled differentiallyby these compartments and their membranes. Rhizophora manglerevealed greater apparent contents of Na than Cl in both thevacuolar and cytoplasmic phases (5.4 and 7.7 fold greater, respectively).The inverse was found for Avicennia germinans, with the apparentvacuolar compartment amount for Cl being 4.6 times greater thanthe Na amount and the cytoplasmic Cl amount was 1.5 times morethan Na contents. The rate constants of efflux showed a patternof significantly greater Na than Cl efflux from each respectivered mangrove compartment, while the black mangroves had a patternof greater efflux rates of Cl than Na in each compartment. Thehalf-times of efflux for Na from the black mangrove cytoplasmiccompartment were 3.5 times longer, while vacuolar half-timesof efflux were near unity for Na and Cl. The results are discussedin terms of Scholander's model of salt secreting and salt excludingspecies. (Received March 26, 1984; Accepted June 26, 1984)     

Alterations in Functional and Structural Connectivity in Pediatric-Onset Multiple Sclerosis

Background

Reduced white matter (WM) integrity is a fundamental aspect of pediatric multiple sclerosis (MS), though relations to resting-state functional MRI (fMRI) connectivity remain unknown. The objective of this study was to relate diffusion-tensor imaging (DTI) measures of WM microstructural integrity to resting-state network (RSN) functional connectivity in pediatric-onset MS to test the hypothesis that abnormalities in RSN reflects changes in structural integrity.

Methods

This study enrolled 19 patients with pediatric-onset MS (mean age = 19, range 13–24 years, 14 female, mean disease duration = 65 months, mean age of disease onset = 13 years) and 16 age- and sex-matched healthy controls (HC). All subjects underwent 3.0T anatomical and functional MRI which included DTI and resting-state acquisitions. DTI processing was performed using Tract-Based Spatial Statistics (TBSS). RSNs were identified using Independent Components Analysis, and a dual regression technique was used to detect between-group differences in the functional connectivity of RSNs. Correlations were investigated between DTI measures and RSN connectivity.

Results

Lower fractional anisotropy (FA) was observed in the pediatric-onset MS group compared to HC group within the entire WM skeleton, and particularly the corpus callosum, posterior thalamic radiation, corona radiata and sagittal stratum (all p < .01, corrected). Relative to HCs, MS patients showed higher functional connectivity involving the anterior cingulate cortex and right precuneus of the default-mode network, as well as involving the anterior cingulate cortex and left middle frontal gyrus of the frontoparietal network (all p < .005 uncorrected, k≥30 voxels). Higher functional connectivity of the right precuneus within the default-mode network was associated with lower FA of the entire WM skeleton (r = -.525, p = .02), genu of the corpus callosum (r = -.553, p = .014), and left (r = -.467, p = .044) and right (r = -.615, p = .005) sagittal stratum.

Conclusions

Loss of WM microstructural integrity is associated with increased resting-state functional connectivity in pediatric MS, which may reflect a diffuse and potentially compensatory activation early in MS.     

The Impact of Sheep Grazing on Net Nitrogen Mineralization Rate in Two Temperate Salt Marshes

Abstract: Nitrogen mineralization rate was studied in grazing trials with three different stocking rates (0, 3, 10 sheep ha^-1) in two man-made salt marshes, viz. a Puccinellia maritima -dominated low salt marsh and a high salt marsh dominated by Festuca rubra. Mineralization rates were derived from the amounts of mineral N which accumulated in situ during six-week incubation periods in tubes containing undisturbed soil cores from the upper 10 cm soil layer. The annual rates of net N mineralization were significantly higher in the better drained, high salt marsh (71 - 81 kg ha^-1 yr^-1) than in the low salt marsh (39 - 49 kg ha^-1 yr^-1). High amounts of belowground litter accumulated in the low salt marsh due to frequent water logging. Both N mineralization and nitrification rate were negatively correlated with soil water content. In the Puccinellia maritima salt marsh, grazing had neither an effect on N mineralization rates during any of the incubation periods nor on annual mineralization rates. In the Festuca rubra salt marsh, N mineralization rates increased earlier during spring at the intensively grazed site than at the moderately grazed and the ungrazed site. N mineralization and nitrification rates were significantly higher at the ungrazed site than at the intensively grazed site during the period of peak net N mineralization from the end of April until mid-June. Although sheep grazing affected the seasonal pattern of N mineralization in the high marsh, grazing did not affect the annual rate of net N mineralization.