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1.
Joseph Orly Yigal Farkash Nitzan Hershkovits Lina Mizrahi Patricia Weinberger 《In vitro cellular & developmental biology. Plant》1982,18(12):980-989
Summary The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian
granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on,
intensive progestin production. However, unlike FSH, OS does not induce accumulation of cyclic AMP (cAMP) in the granulosa
cells. The ovarian factor cannot be cAMP as its action is not abolished by phosphodiesterase (PDE) treatment. Neither is it
a possible PDE inhibitor, as it does not augment cAMP accumulation in granulosa cells or Friend erythroleukemic cells induced
by FSH or PGE1, respectively. The factor is still active after heating for 20 min at 90° C but is rapidly inactivated by alkali treatment.
In addition, treatment with various proteases did not abolish the steroidogenic activity. These findings suggest a possible
novel intraovarian regulator of the granulosa cell function.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This work was supported by the United States-Israel Binational Science Foundation, Grant 2656/81.
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
2.
Ronald Levy Yehudit Bergman Olivera Finn 《In vitro cellular & developmental biology. Plant》1981,17(12):1051-1057
Summary In the present paper, we will summarize studies we have performed on two distinct human lymphocyte cell surface antigens defined
by monoclonal antibodies: Leu-1 and HLA-DR.
Presented in the symposium on The Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association, Washington,
D.C., June 7–11, 1981.
This work was supported by USPHS-NIH Grants CA-21223, AI-11313, and CA-09302.
This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech
Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England
Nuclear Corporation, and Ortho Pharmaceutical Corporation. 相似文献
3.
Laminin provides a better substrate than fibronectin for attachment,growth, and differentiation of 1003 embryonal carcinoma cells 总被引:3,自引:0,他引:3
Michel Y. Darmon 《In vitro cellular & developmental biology. Plant》1982,18(12):997-1003
Summary Culture of cells in hormonally defined media has allowed (a) the demonstration of physiological responses from cells usually
unable to express them in vitro and (b) the study of the effects on growth and differentiation of diffusible factors and attachment
factors. The embryonal carcinoma line 1003 forms multidifferentiated tumors in vivo but is unable to differentiate in vitro
when grown in serum-containing medium. In a defined medium containing insulin, transferrin, selenium, and fibronectin as attachment
factors, 1003 cells grow for several generations and differentiate into neurons and embryonic mesenchyme (Darmon et al., 1981,
Dev. Biol. 85: 463–473). In the present work the effects of fibronectin and laminin were compared. In the presence of laminin
the cells attached and spread better, grew faster, and could be plated at lower densities. Neurite extension was also better
under these conditions and most importantly, it was found that laminin induced an important formation of muscular tissue when
the cells had been seeded at low densities. Multinucleated myotubes could be stained with antibodies directed against embryonic
muscular myosin. Coating the dishes with polylysine or adding FGF or serum-spreading factor to the medium allowed growth of
low-density cultures with fibronectin instead of laminin but muscular differentiation was not detected under these conditions.
Addition of fibronectin to laminin-containing medium did not inhibit muscular differentiation.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This research was supported in part by grants from the “Centre National de la Recherche Scientifique” (LA 269), the “Délégation
Générale à la Recherche Scientifique et Technique,” the Fondation pour la Recherche Médicale Fran?aise,” the “Institut National
de la Santé et de la Recherche Medicale,” the “Ligue Nationale Fran?aise centre le Cancer,” and the “Fondation André Meyer.”
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
4.
Tatsuhiko Ikeda Qi-fu Liu David Danielpour Jefferson B. Officer Masayoshi Iio Frances E. Leland David A. Sirbasku 《In vitro cellular & developmental biology. Plant》1982,18(12):961-979
Summary The role of polypeptide growth factors (estromedins) as mediators of estrogen-responsive mammary tumor growth is studied in
this report. Three possible new mechanisms were investigated that include endocrine, autocrine, and paracrine related growth
factors. The first hypothesis being tested is whether estrogens interact with target tissues and cause the biosynthesis and
secretion of polypeptide growth factors, which then act as mitogens for normal and neoplastic mammary tissues. Data presented
suggest that this mechanism involves estrogen interaction with uterus, kidney, and pituitary gland causing production of growth
factors, which then enter the general circulation and promote growth of distant target tissues. This is an endocrine type
mechanism. Another type of estromedin control (autocrine control) may be exerted in an autostimulatory way in which the target
tissue produces the polypeptide factors for its own growth in response to estrogen stimulation. A variation of the autocrine
mechanism may be a paracrine mechanism in which some cells of an estrogen-responsive normal or neoplastic tissue produce growth
factors that act on adjacent or neighboring cells. From the data available, all three possible types of growth factors could
be functioning synergistically to yield the final result of continuous estrogen responsive tumor growth in vivo.
Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association,
San Diego, California, June 6–10, 1982.
This work was supported by American Cancer Society Grant BC-255; D. A. S. is the recipient of an American Cancer Society Faculty
Research Award, FRA-212. D. D. is supported by a Rosalie B. Hite predoctoral fellowship from the Rosalie B. Hite Foundation,
Houston, Texas.
This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture
Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation. 相似文献
5.
Roger H. Kennett 《In vitro cellular & developmental biology. Plant》1981,17(12):1036-1050
Summary Since the first report of hybridomas producing monoclonal antibodies by Kohler and Milstein in 1975, this technique has spread
to nearly all areas of biological, biochemical, and biomedical research. Watching the use of these methods spread from immunologists
to cell biologists, developmental biologists, biochemists and to other biological disciplines and observing the nearly logarithmic
increase in publications using these reagents has been in itself fascinating and informative. An overview of the development
of this technology and its applications is presented including the use of monoclonal antibodies to study cell surface molecules,
differentiation antigens, receptors, and histocompatibility antigens. The use of these antibodies to analyze microorganisms
and parasitic antigens as well as their use in the genetic analysis of human cell surface antigens and the detection of polymorphic
variation in enzymes and other proteins is discussed. Examples of the application of monoclonal reagents to the study of tumor
cell biology including the labeling of metastatic tumor cells and the detection of cell surface molecules implicated in the
regulation of growth control and cell division are provided.
Presented in the symposium on The Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association. Washington,
D.C., June 7–11. 1981.
This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech
Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England
Nuclear Corporation, and Ortho Pharmaceutical Corporation. 相似文献
6.
John D. Minna Frank Cuttitta Steven Rosen Paul A. Bunn Jr. Desmond N. Carney Adi F. Gazdar Stephen Krasnow 《In vitro cellular & developmental biology. Plant》1981,17(12):1058-1070
Summary We have developed a screening strategy and technology to produce monoclonal antibodies with specificity for human lung cancer
cells. Mice and rats were immunized with well-characterized tissue culture lines of human small cell lung cancer (SCLC), mouse
myeloma x spleen hybrids formed by the technique of Kohler and Milstein, and the resulting culture fluids were screened for
antibody binding phenotype using a radioimmunoassay. To facilitate testing large numbers of culture fluids, a 96-well, microtiter
based, resuable, replicating device was designed. Using this, many hybridoma culture fluids were replica plated for antibody
binding tests on a series of human target cell plates. Hybrids producing antibodies that reacted with the immunizing SCLC
line and another independent SCLC line, but not with autologous B-lymphoblastoid cells derived from one of the patients, were
identified, selected, and then repeatedly recloned using the same screening strategy. With this technology, hybridomas representing
less than 0.5% of all hybrids generated could be isolated and stable antibody producing cultures derived. Such antibodies
reacted with a panel of well-characterized SCLC lines and SCLC samples taken directly from patients but not with a variety
of normal tissues. Using these antibodies we can demonstrate: tumor cell contamination of bone marrow specimens, marked heterogeneity
of antigen expression on cells within individual SCLC lines and individual patients, and inhibition of clonal growth of SCLC
lines in soft agarose assays. All of these findings have potential clinical and cell biologic application.
Presented in the symposium on The Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association, Washington,
D.C., June 7–11, 1981.
This symposium was supported in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech
Incorporated, MAB-Monoclonal Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England
Nuclear Corporation, and Ortho Pharmaceutical Corporation. 相似文献
7.
Norman R. Klinman Kathleen A. Denis Linda A. Sherman 《In vitro cellular & developmental biology. Plant》1981,17(12):1029-1035
Summary It has long been realized that only the study of homogeneous antibodies or cell populations could enable a definitive understanding
of much of the immune mechanism. Hybridoma technology has greatly facilitated such approaches. Hybridoma antibodies have been
used to delineate both B cell and T cell subpopulations. T cell studies per se have been accomplished by the use of T cell
hybridoma cell lines producing a variety of factors. Anti-idiotypes against B cell hybridoma antibodies have been used to
characterize T cell receptors and factors. B cell studies have been facilitated by hybridomas that have made available the
immunoglobulin of pre-B cells or defective B cell lines. Hybridoma antibodies have also been used to dissect closely related
antibody families and the potential for responsiveness against a variety of antigenic determinants. Finally, hybridomas have
provided a primary source of material for protein and DNA sequence analysis. In our laboratories hybridoma antibodies derived
against the murine H-2 locus have demonstrated the ability of B cell antibodies to discriminate amongst H2 mutants—a capacity
previously attributed only to T cell specificities. Hybridoma antibodies have also been generated by fusions with antigen
stimulated neonatal B cells to provide homogeneous antibodies reflective of the earliest developmental immunoglobulin readout.
Such probes should increase our understanding of the processes involved in the generation of both the T and B cell repertoires.
Presented in the symposium on the Biology of Hybridomas at the 32nd Annual Meeting of the Tissue Culture Association. Washington,
D.C., June 7–11, 1981.
This work was supported by United States Public Health Service Grants AI 15797 and AI 15710. This symposium was supported
in part by the following organizations: Bethesda Research Laboratories, Cetus Corporation, Hybritech Incorporated, MAB-Monoclonal
Antibodies, Inc., National Capital Area Branch of the Tissue Culture Association, New England Nuclear Corporation, and Ortho
Pharmaceutical Corporation. 相似文献
8.
Hormonal growth control of cells in culture 总被引:15,自引:0,他引:15
Summary Serum is the last undefined component in cell culture media. Our results indicate that the primary role of serum is to provide
hormones and that serum can be replaced by a group of hormones. A rat pituitary cell line, GH3, can grow in serum-free medium if the medium is supplemented with 3,3′,5-triiodothyronine, TSH-releasing hormone, transferrin,
parathyroid hormone, insulin and three isoelectric focusing fractions of blood meal. The blood-meal components can be replaced
by fibroblast growth factor and somatomedin C. The growth rate of GH3 cells in hormone-supplemented serum-free medium is equal to that in serum-supplemented medium, and subculture in such medium
is also possible. These results indicate that the replacement of the serum component is complete in the GH3 system. The hormonal requirements of GH3 cells and those of HeLa and mouse melanoma, M2R, were compared. Two generalizations could be made: (a) All three cell lines
require insulin and transferrin. (b) There is a requirement for a hormone which localizes in the nucleus for each cell line.
These generalizations seem to hold true for most of the other cell lines for which the hormonal requirements have been partially
worked out. Since insulin is one of the universally required hormones, its effects on GH3, HeLa and M2R were compared. Insulin stimulates glycogen synthesis in all three cell lines and facilitates fatty-acid synthesis
in GH3 and M2R. However, there is a difference in the effect of insulin on growth among the three cell lines. Insulin is an absolute
requirement for GH3 cells without which the cells cannot survive, whereas this is not the case for HeLa and M2R. The most stringent requirement
for HeLa cells is for hydrocortisone, and for M2R, it is for transferrin. These results indicate that even though the necessity
for some hormones is common, the degree of requirement may vary from one cell line to another. Whether this difference reflects
the difference in the primary mode of action of the hormone on each cell type needs further investigation.
Presented in the Opening Symposium on Nutritional Factors and Differentiation at the 28th Annual Meeting of the Tissue Culture
Association, New Orleans, Louisiana, June 6–9, 1977.
This work was supported by NIH Grant GM 17019. J. Larner was supported by Josiah Macy Foundation 相似文献
9.
We established a serum-free medium of low protein content(125g/ml) TYI 100, consisting of three hormones and five growth factors for the growth of lymphoid and hybridoma cell lines. In TYI 100 medium, mouse and human hybridomas grew equally well as in RPMI 1640 supplemented with 10% fetal bovine serum (10% FBS) without adaptation to the serum-free medium. TYI 100 medium allowed several passages of mouse hybridoma lines and the total cell number was more than in 10% FBS. TYI 100 medium also supported growth of myelomas and anchorage dependent cell lines, Bowes and CHO, well. TYI 100 medium is composed of inexpensive supplements and is therefor applicable to large scale culture.Abbreviations FBS
Fetal Bovine Serum
- IMDM
Iscove's Modification of Dulbecco's Medium
- PBS
Phosphate-Buffered Saline
- TPA
Tissue Plasminogen Activator 相似文献
10.
Julien Deschênes Jean-Paul Valet Normand Marceau 《In vitro cellular & developmental biology. Plant》1980,16(8):722-729
Summary The two-step collagenase perfusion method originally developed for the high yield isolation of parenchymal cells from adult
rat livers has been adapted to rats of 1 day, 1 week, and 3 weeks of age. The use of this method to isolate hepatocytes from
five or six rats of the respective ages demonstrated its reliability in terms of cell yield, percentage of single cells, and
cell viability. In all cases, hepatocytes attach with high efficiency to fibronectin precoated dishes using serum-free culture
medium. The dynamics of spreading is faster for newborn hepatocytes than adult ones. The functional integrity of these parenchymal
liver cells was assessed by their capacity to secrete albumin and α-fetoprotein in serum-free medium and to express lactate
dehydrogenase activity over a 24-hr period in primary culture.
Part of this work was presented at the 30th Annual Meeting of the Tissue Culture Association, Seattle, June, 1979. 相似文献
11.
Ding Huang Wen-Juan Peng Qian Ye Xu-Ping Liu Liang Zhao Li Fan Kang Xia-Hou Han-Jing Jia Jian Luo Lin-Ting Zhou Bei-Bei Li Shi-Lei Wang Wen-Ting Xu Ze Chen Wen-Song Tan 《PloS one》2015,10(11)
Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51×106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97×106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted. 相似文献
12.
W. K. Whitten 《In vitro cellular & developmental biology. Plant》1979,15(1):19-22
Summary The genetic background and morphology of spontaneous mosaic hermaphrodites of mice have been described.
Presented in the formal symposium on Sexual Differentiation in Vitro and in Vivo at the 29th Annual Meeting of the Tissue
Culture Association, Denver, Colorado, June 4–8. 1978.
This work was supported by Grant HD 04083 from the National Institute of Child Health and Human Development. 相似文献
13.
Lynne P. Rutzky Beppino C. Giovanella Baldwin H. Tom Celia I. Kaye Philip D. Noguchi Barry D. Kahan 《In vitro cellular & developmental biology. Plant》1983,19(2):99-107
Summary An epithelial cell line, LS123, was established in 1974 from the second in a series of three primary colonic tumors resected
from a Caucasian female. The cell line is aneuploid, releases low concentrations of carcinoembryonic antigen (CEA), fails
to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells. LS123
cells grow on confluent cell monolayers and in either low serum or serum-free medium. In the chick embryonic skin assay, LS123
cells grew as a well-differentiated abnormal colonic epithelium with little mitotic activity but with some indication of invasion.
On floating collagen gels LS123 cells formed a one to three-cell-layer-thick undifferentiated epithelial sheet. The apparent
low invasiveness of the cells of this line is supported by the patient's history of three primary colon tumors without systemic
metastases during the past 30 yr. Therefore, although LS123 cells possess several properties associated with neoplasia, they
have little invasive potential. Thus, LS123 cells may represent an important model for the study of human colon cancer.
Presented in part at the 33rd Annual Meeting of the Tissue Culture Association, San Diego, CA, June 6–10, 1982.
The work has been partially supported by U.S. Public Health Service Grants CA23871 (L. P. R.), CA24024 and RCDAK04-CA00579
(B. H. T.), and CA27124 and CA22370 (B. D. K.); the latter was awarded through the National Large Bowel Cancer Project. 相似文献
14.
The role of junctional communication in animal tissues 总被引:2,自引:0,他引:2
John D. Pitts 《In vitro cellular & developmental biology. Plant》1980,16(12):1049-1056
Summary Permeable intercellular junctions are a common feature of most animal tissues. These junctions allow the free exchange of
small ions and molecules between all the cells in coupled populations. Such limited syncytial interaction contributes to the
integration of individual cells into organized tissues.
Presented in the symposium on Molecular and Morphological Aspects of Cell-Cell Communication at the 31st Annual Meeting of
the Tissue Culture Association, St. Louis, Missouri, June 1–5, 1980.
This symposium was supported in part by Contract 263-MD-025754 from the National Cancer Institute and the Fogarty International
Center. 相似文献
15.
Terry Golombick R. Dansey W. R. Bezwoda Jennifer Rosendorff 《In vitro cellular & developmental biology. Plant》1990,26(5):447-454
Summary The establishment, growth, and characterization of two new continuously growing human ovarian cancer cell lines (UWOV1 and
UWOV2) as, well as a subline (UWOV2, Sf) grown in chemically defined, serum-free medium are described. The cell lines were
derived from ascitic tumors of two patients suffering from cystadenocarcinomas of the ovary. Both UWOV1 and UWOV2 lines grow
in anchorage-dependent fashion as monolayers, whereas UWOV2 (Sf) forms multilayered domelike structures. Cytogenetic studies
revealed nonrandom abnormalities involving chromosomes 1 and 11 in all three cell lines. Secretion of soluble collagen was
detected in all three cell lines. In addition, UWOV2 (Sf) produces and secretes large amounts of extracellular matrix material
with an ordered fibrillar structure which may function as an attachment factor for the serum-free cells. These cell lines
seem to be useful for further studies of the biology of human ovarian cancer.
This research was supported by grants from National Cancer Association (S. A.) and Bekker Trust Foundation. 相似文献
16.
Richard F. Bauer Larry O. Arthur Donald L. Fine 《In vitro cellular & developmental biology. Plant》1976,12(8):558-563
Summary Five different mouse mammary tumor cell lines were propagated in a serum free medium. Evaluation of growth characteristics,
including logarithmic growth, cell population increase, protein production and days to confluency, showed serum-free medium
comparable to serum-containing medium. Mouse mammary tumor virus expression and production, in C3H and GR tumor cell lines, as determined by virus particle counting and RNA dependent DNA polymerase assays, subsequent to
dexamethasone stimulation revealed equivalent to higher levels of virus in serum-free medium as compared to serum-containing
medium. 相似文献
17.
Kiyoshi Higuchi Richard C. Robinson 《In vitro cellular & developmental biology. Plant》1973,9(2):114-121
Summary An improved chemically defined, serum-free medium for the cultivation of a variety of continuous cell lines has been developed.
Eight lines of human origin, three lines of nonhuman primate origin, and five lines of rodent origin have been cultured serially
for as long as a year in the medium. Growth rates of several serial lines resulted in as much as 20- to 30-fold increases
per week. Hormones such as insulin, cortisol, and thyroxine significantly improved growth of cultures in the defined medium.
Vitamin B12 and biotin were required for growth. Lipids such as oleic acid, lecithin, and cholesterol also promoted growth of several
cell lines. Virtually all continuous cell lines tested grew well upon initial transfer into the serum-free defined medium.
Most cell lines could be serially subcultured rapidly with little evidence that selection of rare cell types was necessary
for growth in the defined medium. However, a few cell lines such as the BHK-21 (hamster) cell and the AKR (mouse embryo) cell
required prolonged periods (4 to 8 weeks) of culturing before rapid growth occurred. Primary cell cultures and other diploid
cells such as human fibroblast (strain WI-38) could not be subcultured successfully in the present medium. 相似文献
18.
D. Gospodarowicz G. Greenburg H. Bialecki B. R. Zetter 《In vitro cellular & developmental biology. Plant》1978,14(1):85-118
Summary The control of proliferation of mesoderm-derived cells by EGF and FGF has been examined taking, as an example, the vascular
endothelium. The mechanisms by which cell proliferation can be brought to a stop in vivo and in vitro have been reviewed.
Presented in the formal symposium on Mechanisms of Cellular Control at the 28th Annual Meeting of the Tissue Culture Association,
New Orleans, Louisiana, June 6–9, 1977.
This work was supported by Grants HL20-197 and HD 11082 from the National Institutes of Health, and VC-194 from the American
Cancer Society. B. R. Zetter was supported by Fellowship 5-F32-CA05149-02 from the U.S. Public Health Service. 相似文献
19.
Thomas B. Shea Eugene S. Berry 《In vitro cellular & developmental biology. Plant》1983,19(11):818-824
Summary An undefined, serum-free medium was developed for use with fish cell cultures. Lactalbumin hydrolyzate, trypticase-soy broth,
Bacto-peptone, dextrose, yeastolate, and polyvinylpyrrolidone were initially combined in 100 ml of distilled H2O, autoclaved, and added to 5% of the final volume of Medium 199. In addition, filter sterilized bovine pancreatic insulin,
glutamine, and nonessential amino acids were added to the medium. The addition of insulin was observed to be unnecessary.
Five fish cell lines [goldfish-derived CAR cells, fathead minnow (FHM) cells, epithelioma papillosum cyprini (EPC) cells,
chinook salmon embryo (CHSE-214) cells, and a new cell line from goldfish air bladders (ABIII)] were all capable of growth
in the serum-free medium at rates equivalent to cells grown in fetal bovine serum (FBS). The morphology of all cell lines,
except CHSE-214 cells, was identical to cells grown in FBS. All cell lines were capable of long-term growth in the serum-free
medium. The CAR, ABIII, EPC, and CHSE-214 cells in the serum-free medium supported the replication of goldfish virus-2 at
levels equivalent to cells grown in FBS. 相似文献
20.
Transferrin (TF) promotes the cell growth of two non-tumorigenic mouse testicular-derived cell lines (TM1 and TM3) when cultured in a low-iron serum-free culture medium. No species specificity for growth promotion was observed using mouse, rat, and human TF. Stimulation of growth by apo-TF was biphasic reaching a maximum at 5.0–20.0 μg/ml and declining at higher concentrations. No decrease in TF-stimulatory effect was observed when high doses of diferric TF (Fe-TF) were used, nor when high doses of apo-TF were added in a medium supplemented with additional iron. The decrease in growth-promoting effects of high doses of apo-TF was not restored by the addition of other metals bound by TF indicating that sequestration of these metals was not responsible for the inhibition of growth. The decrease in growth was observed with high levels of apo-TF even in the presence of doses of Fe-TF which elicited a maxima) growth response, supporting the hypothesis that the apo-TF in this system is competing for Fe-TF binding sites and thus diminishing the delivery of Fe to the cells. The growth-promoting effect of apo-TF is blocked when the iron content of the medium is sequestered by Deferoxamine (DFX). However, under these conditions Fe-TF stimulates growth. These observations support the hypothesis that the growth-promoting effect of TF can be accounted for by its role in providing iron to the cells. 相似文献