Selected chromosome counts of the Czechoslovak flora III

Chromosome numbers compared with as yet published data are given for the following 12 Phanerogams (both native species and aliens) from Czechoslovakia:Ambrosia trifida L.,Cardamine chelidonia L.,Dephne cneorum L.,Epipactis albensis Nováková etRydlo,Linum flavum L.subsp flavum, Lunaria rediviva L.,Nepeta grandiflora M.BIEB.,Reseda luteola L.,Thlaspi montanum L.,Tithymalus salicifolius (Host)Klotzsch etGarcke,Tithymalus virgultosus (Klokov) Holub andVerbascum speciosum Schrad. subsp.speciosum. The chromosome number 2n=40 is presented for the first time in autogamousEpipactis albensis Nováková etRydlo. New chromosome numbers were found inCardamine chelidonia L. (2n=32) and inTithymalus salicifolius (Host) Klotzch etGarcke (2n=40). Known but less frequent cytotypes are reported inLinum flavum L. subsp.flavum (2n=28) and inVerbascum speciosum Schrad. subsp.speciosum (2n=30).     

Myosotis michaelae — A new species ofMyosotis ser.Palustres (Boraginaceae)

A new species ofMyosotis ser.Palustres Popov,Myosotis michaelae, related to theM. nemorosa group, is described from France. Further localities from Switzerland, Romania and Bulgaria are listed. Diploid chromosome number (2n=22) is given for the new species, and notes on its distribution, ecology and taxonomic relationships are presented. The lectotype is chosen and designated for the nameMyosotis palustris L. var.scabra Simonk.     

Chromosome counts of some Cuban Angiosperms

Chromosome numbers are reported for 14 species collected in Cuba. The first chromosome records are reported forAlbizzia cubana Britton etWilson (2n=26),Atkinsia cubensis (Britton etWilson)Howard (2n=26),Caesalpinia violacea (Mill.)Standl. (2n=24),Colubrina ferruginosa Brongn. (2n=24). Chromosome numbers of the following species were confirmed:Albizzia lebbeck (L.)Benth. (2n=26),Canavalia maritima (Aubl.)Thouars (2n=22),Casuarina equisetifolia Forst. (2n=18),Cedrela mexicana M.J. Roem. (2n=56),Delonix regia (Bojer)Raf. (2n=28),Guazuma tomentosa H.B.K. (2n=16),Lysiloma bahamense Benth. (2n=26),L. latisiliqua (L.)Benth. (2n=26),Samanea saman (Jacq.)Merrill (2n=26),Thespesia populnea (L.)Soland (2n=26).     

Annotated chromosome numbers of selected asiaticPotentilla species

Chromosome numbers are reported for 19 collections representing 16 AsiaticPotentilla taxa. The first chromosome records are reported forP. desertorum Bunge var.arnavatensis Wolf (2n=28),P. festiva Soják (2n=28),P. griffithii Hook f. subsp.beauvaisii (Cardot) Soják (2n=42),P. micropetala D. Don subsp.byssitecta (Soják) Měsí?ek etSoják (2n=14),P. mollissima Lehm. (2n=28),P. moorcroftii Wall. exLehm. (2n=42),P. multicaulis Bunge (2n=14),P. [x]omissa Soják (2n=35, 56, 70) andP. stanjukoviczii Ovcz. exKoczk. (2n=14). Counts differing from those previously recorded are given forP. algida Soják (2n=56) andP. flagellaris Willd. exSchlecht. (2n=42). Chromosome numbers of the following species were confirmed:P. [x]agrimonioides Bieb. (2n=42),P. chinensis Ser. in DC. (2n=14),P. fragarioides L. (2n=14),P. lineata Trev. (2n=14) andP. sericea L. (2n=28). Taxonomy is briefly discussed. A new combinationP. micropetala D. Don subsp.byssitecta (Soják) Měsí?ek etSoják stat. nov. is proposed.     

Chromosome counts of some MongolianPotentilla species

Chromosome numbers are reported for 26 collections representing 12 MongolianPotentilla taxa. The first chromosome records are reported forP.[x]agrimonioides Bieb. (2n = 42, 49–50),P. [x]chionea Soják (2n = 64, 74+1B, 84–86, 86),P. [x]stepposa Soják (2n = 62) andP. supina, L. subsp.costata Soják (2n = 42). Tetraploid and pentaploid populations ofP. sericea L. (2n = 28, 34, 35) and hexaploid populations ofP. omithopoda Tausch (2n = 42) were found in Mongolia. Chromosome numbers of the following species were confirmed:P. longifolia Willd. exSchlecht. (2n = 14),P. multifida L. (2n = 28),P. sanguisorba Willd. exSchlecht. (2n = 28),P. sericea L. (2n = 42),P. supina L. subsp.paradoxa (Nutt.) Soják (2n = 28 China) andP. tanacetifolia Willd. exSchlecht. (2n = 28). Taxonomic observations are briefly discussed.     

Untersuchungen über den PolyploidkomplexVeronica cymbalaria agg. (Scrophulariaceae)

Veronica panormitana Tin. inGuss. (sparsely distributed from Corsica and Sicily to Syria) andV. trichadena Jord. etFourr. var.freyniana M. Fisch. (Mallorca) are diploid (2n = 18), and very probably the ancestors of the allopolyploidV. cymbalaria Bod. The latter consists of tetraploid (2n = 36) and hexaploid (2n = 54) strains (confirmed on new material from Italy, Greece, and Turkey). The diploid taxa are morphologically well separated from each other and fromV. cymbalaria, whereas the tetraploids and hexaploids are not separable morphologically.—Another poorly known diploid strain from Kurdistan is described as new:Veronica cymbalaria Bod. var.baradostensis M. Fischer.—V. panormitana is reported from Greece for the first time.     

Chromosome numbers in selectedHieracium species in the Krkonoše Mts. (the West Sudeten)

Chromosome numbers are given of 15 species of the genusHieracium L. s. str., representing seven species groups (in the sense of Flora Europaea, roughly corresponding to Zahn's “species principales”) from the Krkono?e Mts., N. Bohemia and SW Poland. For the first time chromosome numbers are reported forH. melanocephalum Tausch (2n=27),H. tubulosum Tausch (2n=36),H. schustleri Zlatník (2n=36),H. fritzei F. Schultz (2n=27),H. rohlenae Zlatník (2n=27),H. nigrescens Willd. (2n=36),H. decipiens Tausch (2n=36),H. atrellum Juxip inSchischkin etBobrov (2n=27),H. subnigrescens (Fries exNorrlin)Dahlst. (2n=36),H. sudeticum Sternberg (2n=36),H. pedunculare Tausch (2n=36),H. glandulosodentatum Uechtr. (2n=36),H. wimmeri Uechtr. (2n=27). InHieracium alpinum L. s. str. the number 2n=27 has been confirmed. The results show a high proportion of tetraploid taxa; no diploids have been found.     

New chromosome counts in nine endemic species from Iran

Original meiotic chromosome counts are presented for nine endemic species in seven families of Angiosperms from Iran including:Arum giganteum Ghahr. (Araceae) (n=14),Caccinia actinobole Bunge (Boraginaceae) (n=8),Delphinium aquilegifolium (Boiss.)Bornm. (Ranunculaceae) (n=8),Diplotaenia damavandica Mozaff., Hedge etLamond (Apiaceae) (n=11),Gypsophila caricifolia Boiss. (Caryophyllaceae) (n=17),Iphiona arachnoidea (Boiss.)Anderb. (Asteraceae) (n=9),Moltkia gypsacea Rech. f. etAellen (Boraginaceae) (n=20),Onobrychis gaubae Bornm. (Fabaceae) (n=8) andOnosma platyphyllum Riedl (Boraginaceae) (n=9). Eight counts are reported for the first time. Furthermore, the previous chromosome count forIphiona aracnoidea is corrected. Based on cytological data the species status ofMoltkia gypsacea is confirmed; it is not merely synonymous withM. coerulea (Willd.)Lehm. The basic chromosome number n=11 is reported in the genusDiplotaenia for the first time.     

Further karyological studies of the Mongolian flora

The paper gives the chromosome number for 50 species of the Mongolian flora. Some of the counts appear to be the first findings for the species. They are:Draba eriopoda Turcz. exLedeb. 2n=16,Lychnis sibirica L. 2n=24,Papaver pseudocanescens M. Popov 2n=42,Thymus baicalensis Serg. 2n=28 andViola gmeliniana Schultes 2n=24.     

LignicolousHyphomycetes from Czechoslovakia 2.Bactrodesmium

Six species of the genusBactrodesmium M. C. Cooke are described in this paper from Czechoslovakia:B. betulicola M.B. Ellis,B. obovatum (Oudemans) M.B. Ellis,B. spilomeum (Berk. etBr.)Mason etHughes including three new speciesB. globosum Hol.-Jech.,B. pyriforme Hol.-Jech. andB. submoniliforme Hol.-Jech.     

The lipopolysaccharides (O-antigens) of Rhodopseudomonas viridis

The present paper deals with the isolation, and chemical and serological characterization of the O-antigens (lipopolysaccharides, LPS) of the photosynthetic gram-negative bacterium Rhodopseudomonas viridis. The LPS are extractable with hot phenol/water, but unlike the phenol-soluble LPS of the closely related species Rhodopseudomonas palustris, the R. viridis O-antigens are preferentially extracted into the water phase. A mixture of phenol/chloroform/petroleum ether (PCP-method) does not extract the R. viridis LPS. All R. viridis LPS investigated belong to the same chemotype, the polysaccharide moiety of these O-antigens being composed of 3-O-methyl-l-xylose, 3-O-methyl-d-mannose, d-mannose, d-galactose, d-glucose, in addition to 2-keto-3-deoxyoctonate (KDO), glucosamine, 6-deoxyglucosamine (quinovosamine) and galactosamine uronic acid. The R. viridis O-antigens are clearly distinguishable from the l-glycero-d-mannoheptose containing O-antigens of R. palustris by the lack of this sugar (and of any other heptose) in the R. viridis LPS. The lipid moiety (lipid A) of the R. viridis O-antigen can be split off from the LPS by mild acid hydrolysis. Like lipid A from R. palustris, it differs remarkably from the well known lipid A of Enterobacteriaceae, in that d-glucosamine is replaced by a recently identified 2.3-diamino-2.3-dideoxyhexose in the R. viridis and R. palustris lipid A. Unlike enteric lipid A the R. viridis lipid A is phosphate-free and includes as the only fatty acid β-C₁₄OH which is exclusively amide-linked. All R. viridis strains belong to the same serotype so far as investigated, as shown by passive hemagglutination with the isolated O-antigens and rabbit antisera against heat-killed cells.     

Karyological and taxonomic observations onDracocephalum foetidum Bunge andKoenigia islandica L.

Chromosome numbers are reported for two Mongolian species,Dracocephalum foetidum Bunge (2n=12) andKoenigia islandica L. (2n=14). The relationship ofD. foetidum toD. moldavica L. (2n=10) and some patterns of phenotypic variation inK. islandica are briefly discussed. The following new combinations are proposed:K. cyanadra (Diels) Měsí?ek etSoják,K. forrestii (Diels) Měsí?ek etSoják,K. hubertii (Lingelsh.) Měsí?ek etSoják, andK. nummularifolia (Meisn.) Měsí?ek etSoják.     

Chromosome counts and chromosome morphology of some selected species

In the years 1976–1981 we studied chromosome counts and karyotypic formulae of the following 29 species of plants from 41 localities (of these 6 from Bohemia, 32 from Moravia, 3 from Slovakia):Batrachium baudotii (Godron) F. W. Schultz,Chenopodium rubrum L.,C. polyspermum L.,C. murale L.,C. ficifolium Sm.,C. opulifolium Schrader ex DC. inLam. et DC.,C. strictum Roth [subsp.strictum, subsp.glaucophyllum (Aellen)Aellen inJust etAellen, subsp.striatiforme Uotila],Arenaria grandiflora L.,Illecebrum verticillatum L.,Spergula morisonii Boreau inDuchartre,Spergularia marginata (DC. inLam. et DC.)Kittel S. marina (L.)Griseb.,S. rubra (L.) J. etC. Presl,Silene conica L.,Sisymbrium loeselii L.,S. volgense Bieb. exE. Fourn.,S. orientale L. [subsp. orientale, subsp.macroloma (A. Pomel)Dvo?ák],S. officinale (L.)Scop.,Descurainia sophia (L.)Webb exPrantl inEngler etPrantl,Nasturtium officinale R. Br. inAiton,Barbarea arcuata (Opiz inPresl J. et C.)Reichenb.,Lunaria annua L.,Soldanella montana Willd.,S. carpatica Vierh. inUrban etGraebner,Lotus tenuis Waldst. etKit. exWilld.,L. uliginosus Schkuhr,Trigonella monspeliaca L.,Geranium sibiricum L.,Lactuca tatarica (L.)C. A. Meyer.     

Indian species of the genusMirufens [Hym.: Trichogrammatidae]

An account is given of 4 new species ofMirufens Girault:M. brevifuniculata Khan & Shafee, M.albiscutellum Khan & Shafee,M. magniclavata Khan & Shafee andM. longiclavata Khan & Shafee. A key to Indian species of the genusMirufens is also provided.     

Karyotaxonomische Untersuchungen anPotentilla taurica s.l. in Bulgarie

Several taxa considered by previous authors as intraspecific ofPotentilla taurica s.l. are considered as separate species. A diploid chromosome number 2n=14 and the characteristic chromosomes of karyotypes of 3 Balkan endemicsP. ni?i?i Adam.,P. emilipopii Nyarády andP. pirotensis (Borb.) Mark. are recorded.P. bornmuelleri Borb.,P. mollicrinis (Borb.) Stankov andP. astracanica Jacq. are widespread in South-eastern Europe. Of this groupP. mollicrinis is a diploid (2n=14),P. bornmuelleri is tetraploid and inP. astracanica a polyploid serie of 2x=14, 4x=28, 5x=35 and 6x=42 has been found. The karyotypes of all species are characterized by submetacentric chromosomes.     

Survey and distribution ofCybocephalidae [Coleoptera] in Israel

Nine species ofCybocephalus were found in a survey conducted throughout Israel:C. micans (Reitter),C. nigriceps nigriceps (J. Sahiberg),C. nigriceps sinister Endrödy-Younga,C. aegyptiacus Endrödy-Younga,C. mediterraneus Endrödy-Younga,C. pullus Endrödy-Younga,C. fodori minor Endrödy-Younga,C. membranaceus (Reitter) andCybocephalus sp. n.C. micans andC.n. nigriceps are the most prevalent: the former in the Coastal Plain on citrus and the latter in the Arava region on date palms. In most cases the population density of the predator appeared to be related to that of the prey. Armoured scale insects(Homoptera: Diaspididae) were the main prey ofCybocephalus.     

The trilobiteProtostygina and the composition of the Styginidae, with two new genera

The Styginidae is regarded as an exclusively Ordovician family of trilobites, separate from the Scutelluidae. The hitherto poorly known genusProtostygina Prantl &P?ibyl, 1949 is revised. It is recorded with certainty only from the Llanvirn of the Czech Republic, and the type species is a senior synonym of“Raymondaspis” rubensi rubensi P?ibyl &VANěK, 1968 and“R.” rubensi lybar ?najdr, 1976. Two new styginid genera are proposed:Cyrtocybe, with type species“Raymondaspis” turgida Whittington, 1965, is known from the upper Arenig and lower Llanvirn of Newfoundland, Maine and Norway; andPromargo, with type speciesP.forteyi n. sp., occurs in the Arenig of Newfoundland and Spitsbergen.Turgicephalus Fortey, 1980 is regarded as a junior synonym ofRaymondaspis P?ibyl inPrantl &P?ibyl, 1949. Three genera are excluded from the Styginidae:Kirkdomina Tripp, 1962,Pseudostygina Zhou inZhou et al., 1982 andStyginella P?ibyl &Vaněk, 1971.     

Selected chromosome counts of the Czechoslovak flora II

Chromosome numbers are presented in the following 16 Phanerogams of the Czechoslovak flora:Amaranthus blitoides S. Watson,Anthoxanthum alpinum Á. Löve etD. Löve Acter amellus L.,Bistorta major S. F. Gray,Cardamine hirsuta L.,Cardamine parviflora L.,Chrysosplenium oppositifolium L.,Dactylis glomerata L.subsp. aschersoniana (Graebner) Thell.,Elatine hydropiper L. s. l.,Geranium phaeum L.,Hieracium lachenalii C. C. Gmel.,Jovibarba sobolifera (Sims) Opiz,Kickxia spuria (L.)Dum.,Persicaria hydropiper (L.)Spach,Sedum alpestre Vill. andTephroseris crispa (Jacq.) Schur. Six of them are reported for the first time from Czechoslovakia, the others are mostly first reports of chromosome numbers from the Bohemian and Moravian regions. A new diploid number of 2n=36 is given inElatine hydropiper L. s. l. All results are compared with as yet published data.