Effect of electron-cyclotron resonance heating conditions on the local parameters of short-wavelength plasma turbulence in the L-2M stellarator

Changes in the energy spectra of short-wavelength (k _s ≈ 35 cm^?1) plasma density fluctuations in the local region of the plasma column (r/a = 0.5–0.6) of the L-2M stellarator were studied by the method of collective scattering of 150-GHz radiation. The plasma was heated at the second harmonic of the electron gyrofrequency, the microwave heating power being in the range of 90–170 kW. A sector limiter was introduced in the peripheral plasma (r/a ≥ 0.8), and the Shafranov shift of the magnetic axis was varied by varying the vertical magnetic field. The results of measurements were averaged over 9–16 discharges. It is found that an increase in the heating power and/or the introduction of the sector limiter in the plasma lead to an increase in the energy of density fluctuations, which correlates with a decrease in the plasma energy lifetime. In the spectra of fluctuations, a broad spectral band in the range of 3–50 kHz was observed in which the spectral density was one order of magnitude higher than in the rest of the spectrum. Analysis of the Fourier spectra showed that the introduction of the sector limiter in the plasma resulted in an increase in both the spectral density of fluctuations in the range of 3–50 kHz and the fraction of quasi-coherent structures in turbulent density fluctuations.     

Toroidal inhomogeneity of plasma density fluctuations during ECR plasma heating in the L-2M stellarator

Correlation between short-wavelength (k_⊥ ≈ 20–30 cm^–1) and long-wavelength (k_⊥ ≈ 1–2 cm^–1) plasma density fluctuations in two poloidal cross sections of the stellarator chamber separated by 1/14 or 5/14 of the torus perimeter was studied using collective scattering of radiation of two 75-GHz gyrotrons and radiation of a 37-GHz Doppler reflectometer at an ECR heating power density of 1.6–3.2 MW/m³. It is found that excitation of turbulent fluctuations is bursty in character and that fluctuations excited in different L-2M cross sections are uncorrelated. It is shown that the energy of turbulent fluctuations is modulated by a low frequency of 5–20 kHz. An idea is put forward that anomalous transport is toroidally inhomogeneous.     

Variation of frequency spectrum of the erythrocyte flickering caused by aging,osmolarity, temperature and pathological changes

Frequency spectra of the surface undulations (flickering) of erythrocyte plasma membranes are measured by direct spectral analysis of the intensity fluctuations of the light passing the cells in a phase contrast microscope. Spectra are taken as a function (1) of the temperature (2) of the viscosity and osmolarity of the outer medium (3) of the aging of cells and (4) of pathological transformations. The spectra are approximately superpositions of two Lorentzian lines. At large frequencies,f, the spectra follow f^?2. This behaviour can be interpreted in terms of cell thickness fluctuations caused by thermally excited membrane undulations provided the range of wavelengths is small. The undulations are determined by the membrane curvature elasticity while the lateral tension is negligibly small for cells of discoid shape. The technique presented allows accurate measurements of relative curvature (bending) elastic constants. The spectra of freshly drawn cells are remarkably reproducible. Aging of the cells in the medium leads to an increase in the curvature elastic constant. A decrease in osmolarity causes a reduction in the intensity and line width of the spectra and the flickering vanishes if the cell approaches a spherical shape. The effect of temperature between 10 and 40°C is astonishingly small with the exception of a sudden increase in the amplitude with increasing temperature at 35°C. The flicker spectra of a large fraction of the cells from patients suffering from cronical alcoholism exhibit a reduced line width or an increase in the curvature elastic constant.     

Backscattering of gyrotron radiation and short-wavelength turbulence during electron cyclotron resonance plasma heating in the L-2M stellarator

Backscattering of gyrotron radiation (θ = π) by short-wavelength density fluctuations (k _⊥ = 30 cm^?1) in the plasma of the L-2M stellarator was studied under conditions of electron cyclotron resonance (ECR) plasma heating at the second harmonic of the electron gyrofrequency (75 GHz). The scattering of the O-wave emerging due to the splitting of the linearly polarized gyrotron radiation into the X- and O-waves was analyzed. The signal obtained after homodyne detection of scattered radiation is a result of interference of the reference signal, the quasi-steady component, and the fast oscillating component. The coefficients of reflection of the quasi-steady component, R ₌ ² (Y), and fast oscillating component, R _～ ² (Y), of scattered radiation are estimated. The growth of the R _～ ² (Y) coefficient from 3.7 × 10^?4 to 5.2 × 10^?4 with increasing ECR heating power from 190 to 430 kW is found to correlate with the decrease in the energy lifetime from 1.9 to 1.46 ms. The relative density of short-wavelength fluctuations is estimated to be 〈n _～ ² 〉/〈n _e ² 〉 = 3 × 10^?7. It is shown that the frequencies of short-wavelength fluctuations are in the range 10–150 kHz. The recorded short-wavelength fluctuations can be interpreted as structural turbulence, the energy of which comprises ～10% of the total fluctuations energy. Simulations of transport processes show that neoclassical heat fluxes are much smaller than anomalous ones. It is suggested that short-wavelength turbulence plays a decisive role in the anomalous heat transport.     

Measurement of the fractional oxygenation of leghemoglobin in intact detached pea nodules by reflectance spectroscopy

A method is presented for the rapid measurement of the spectral properties of detached nodules of pea (Pisum sativum L. cv “Sparkle”) by diffuse reflectance spectroscopy. After correcting the spectra for surface light scattering, the spectrum of leghemoglobin is obtained. From this, the fractional oxygenation of leghemoglobin and the internal O₂ concentration can be calculated. With this method, we determined internal O₂ while measuring nitrogenase activity (C₂H₂) in detached pea nodules over a range of external O₂ concentrations. Nitrogenase activity was maximum when leghemoglobin was 25% oxygenated, corresponding to a calculated free O₂ concentration of 45 nanomolar in infected cells. Advantages of this method over previous methods which employed transmitted light are: (a) many nodules can be assayed simultaneously, (b) nitrogenase activity (C₂H₂) can be determined at the same time as spectra are recorded, and (c) spectra can be obtained from nodules submerged in buffer containing metabolic effectors.     

Effect of the transverse magnetic field on turbulence and parameters of a plasma column in the L-2M stellarator

The influence of magnetic configurations with magnetic hills or wells on the parameters of a plasma column and turbulence characteristics were studied in experiments in which the plasma was created and heated by a microwave beam at the second harmonic of the electron cyclotron frequency. Calculations show that, for 〈β〉=(1.5?2)×10^?, a configuration with a magnetic well takes place and the Mercier criterion for stability of the ideal MHD modes is satisfied. It is shown that the compensation of the Shafranov shift of the plasma column by a transverse (vertical) field (B _v /B ₀ =5×10^?3) leads to a configuration with a magnetic hill in which the Mercier stability criterion is violated in the central region of the plasma column. It is experimentally shown that the stored plasma energy in the magnetic-hill configuration is reduced by one-half in comparison with the magnetic-well configuration. In the case of a magnetic hill, the energy of fluctuations increases both in the plasma core and near the separatrix, and the quasi-regular components of the wavelet spectra grow. When the Shafranov shift is compensated only partially (B _v/B ₀～3×10^?3) and the system is near the instability threshold, the stored plasma energy and the central electron temperature are somewhat higher, and the radiation power of fast electrons from non-Maxwellian tails at the second harmonic of the electron gyrofrequency decreases. It is found that the wavelet spectra of fluctuations change, the coherence coefficient for spectral components increases, and the radial electric field near the separatrix decreases.     

Displacement of the electron cyclotron resonance heating region and time evolution of the characteristics of short-wavelength turbulence in the 3D magnetic configuration of the L-2M stellarator

Reflection of the heating extraordinary microwave incident obliquely onto the surface of the electron cyclotron resonance (ECR) at the second harmonic of the electron gyrofrequency in the 3D magnetic configuration of the L-2M stellarator was studied experimentally. The plasma was heated using two gyrotrons with a total power of 600–700 kW, the specific heating power being 2.4–2.8 MW/m³. The displacement of the ECR region in the course of heating was monitored by measuring the phase of the reflected extraordinary wave. It is found that the growth of the plasma density is accompanied by the displacement of the ECR heating region from the center of the plasma column toward its periphery. The coefficient of reflection of the heating microwave beam from the ECR region was measured. The spectra of short-wavelength (k _s ≈ 30 cm^?1) plasma density fluctuations were explored by analyzing backscattered microwave radiation. A tenfold increase in the energy of short-wavelength density fluctuations and the growth of the spectral density of fluctuations in the frequency range of 0.3–1.5 MHz were observed.     

Analysis of the efficiency of lower hybrid current drive in the FT-2 tokamak

Results are presented from experimental studies of the efficiency of lower hybrid current drive (LHCD) in the FT-2 tokamak. The dependence of the LHCD efficiency on the grill phasing Δφ and RF oscillator power was determined experimentally in a wide range of plasma densities. It is shown that, at high plasma currents (i.e., at sufficiently high electron temperatures), current drive is suppressed when the plasma density reaches its resonance value n _LH for the pumping wave frequency, rather than when parametric decay comes into play (as was observed in regimes with lower plasma currents and, accordingly, lower electron temperatures T _e ). In order to analyze the experimentally observed effect of LHCD and its dependence on the value and sign of the antenna phasing, the spectra of the excited LH waves, P(N _z ), were calculated. Simulations using the FRTC code with allowance for the P(N _z ) spectrum and the measured plasma parameters made it possible to calculate the value and direction of the LH-driven current, which are determined by the spectrum of the excited LH waves. It is shown that the synergetic effect caused by the interaction between different spectral components of the excited RF wave plays a decisive role in the bridging of the gap in the wave spectrum.     

Structural characterization of the head-to-head isomers of the [Pd2(Ph2Ppy)2Cl2] and [PtPd(Ph2Ppy)2I2] complexes (Ph2Ppy = 2-(diphenylphosphino)pyridine)

The molecular structures of the thermodynamically unstable head-to-head isomers, HH-[Pd₂(Ph₂Ppy)₂Cl₂] and HH-[PtPd(Ph₂Ppy)₂I₂], have been determined by single crystal X-ray diffraction. The two complexes have proved to be isostructural. The severe distortions of the bond angles from the ideal square planar geometry around the metal centers ligating the trans phosphorus donor atoms are indicative of a more pronounced internal strain in the HH isomers as compared to the HT counterparts. The enhanced internal strain is thought to be the major driving force responsible for the spontaneous conversion of the head-to-head isomers to their head-to-tail congeners. ¹³C NMR spectra in solution phase as well as solid-state ³¹P MAS NMR spectra have proved to be informative regarding the orientation of the asymmetric Ph₂Ppy ligands.     

Light scattering spectroscopy of the squid axon membrane

Light scattering studies on the giant squid axon were done using the technique of optical mixing spectroscopy. This experimental approach is based on the use of laser light to detect the fluctuations of membrane macromolecules which are associated with conductance fluctuations. The light scattering spectra were similar to the Lorentzian-like behavior of conductance fluctuations, possibly reflecting an underlying conformational change in the specific membrane sites responsible for the potassium ion conductance. The amplitude of the spectra measured, increased when the membrane was depolarized and decreased on hyperpolarization. The spectra were fit to the sum of two terms, a (1/fcomponent and a simple Lorentzian term. Spectra from deteriorating axons did not show sensitivity to membrane potential changes. It is shown theoretically that fluctuations due to the voltage-dependent variable, n, of the Hodgkin-Huxley formalism are identical to the voltage fluctuations. The derived power spectrum is that of a second order system, capable of showing resonance peaking only if the voltage dependence of the potassium rate constants is included in the analysis. The lack of resonance peaking in the observed light scattering spectra, indicates that the data are best described by a damped second order system.     

Spectral and statistical analysis of fluctuations in the SOL and diverted plasmas of the Uragan-3M torsatron

In the l = 3/m = 9 Uragan-3M (U3-M) torsatron (R ₀ = 1 m, ā ≈ 0.12 m, ι(ā)/2π ～ 0.3) with an open helical divertor and a plasma produced and heated by RF fields (ω ? ω_ci), studies of low frequency (5–100 kHz) density and potential fluctuations in the SOL plasma and in the diverted plasma flows (DPFs), have been carried out. It is shown, that in the SOL to more (less) distantly located points relative to the last closed magnetic surface, higher (lower) frequency fluctuations are inherent. Such a spectral splitting in two sub-ranges occurs in the DPFs too. A peculiarity of the spatial distribution of DPF fluctuation spectra is that lower frequency fluctuations dominate on the ion toroidal B × ?B drift side. During L-H-like transition in U-3M simultaneously with strong E _r shear formation, a suppression of lower frequency fluctuations and a decrease of local radial turbulent particle flux take place. Results are presented of investigation of plasma density fluctuations in SOL with the use of probability distribution function (PDF) analysis. Evaluations of skewness and kurtosis of fluctuations have been made. The analysis of I _s fluctuations in DPF have been carried out in a similar way.     

Spectra of langmuir waves in a magnetized plasma with low-frequency turbulence

A study is made of the formation of the spectra of Langmuir waves excited as a result of the development of beam-plasma instability in a collisionless magnetized plasma with low-frequency turbulence. Equations are derived that describe the dynamics of the formation of spectra in the quasilinear statistical approximation.The equations obtained account for small-and large-angle scattering of the electron-beam-excited waves by given background plasma density fluctuations. The scattering of Langmuir waves leads to the redistribution of their energy in phase space and, under appropriate conditions, to the appearance of a characteristic dent in the wave spectra in the frequency range where the spectral intensity is maximum. Numerical simulations carried out for plasma parameters typical of the polar cap of the Earth’s magnetosphere help to explain the shape of the spectra of Langmuir waves that were recorded by the Interball-2 satellite when it was flying through this magnetospheric region.     

Quasi-elastic light scattering by diffusional fluctuations in RNase solutions

Using measurements of quasi-elastic light scattering spectra, we have investigated diffusional fluctuations of RNase. The diffusion coefficient for individual protein molecules, together with the corresponding calculated effective molecular radius R_eff, were determined. Between room temperature and the point of irreversible denaturation at 63.5°C, R_eff increased from 20-250 A. This is comparable to the plateau in R_eff of 300 A reached after about 200 min following chemical denaturation in 10 M urea. The measurements indicated the presence of a large size component even in the freshly prepared and chromatographically purified solutions. From the diffusion constants deduced for this large component we obtained effective sizes from 1000-5000 A. Concentration and temperature dependent measurements exclude the possibility that these large particles are impurities and indicate that they are the result of aggregations of RNase molecules.     

Use of additional helium puffing for the diagnostics of plasma parameters at the FT-2 tokamak

The experiments carried out at the FT-2 tokamak in which additional pulsed puffing of helium into the hydrogen plasma was used for diagnostic purposes are considered. To estimate the necessary content of helium ions in the experiments on studying short-scale plasma oscillations, the ionization-recombination balance was simulated numerically under the assumption of a toroidally homogeneous influx of the working gas onto the boundary of the plasma column. In these simulations, the effective density of the neutral gas incident on the plasma boundary was determined by the iteration method, which made it possible to provide agreement between the obtained solution and the experimental discharge conditions. In particular, the correspondence of the determined admixture content to both the plasma quasineutrality condition and the value of the effective charge Z _eff, as well as agreement between the calculated and measured plasma density profiles, was ensured. The simulations were performed under the assumption of anomalous diffusion coefficients for all plasma components. The temporal variations of the ionization-recombination balance were checked by comparing them with the measured spectra of radiation in the HeI, HeII, and H_?? lines. In the current drive experiments, variations in n _e (r) at the discharge periphery were examined by the method based on the proportionality of the intensity ratio of the helium spectral lines, HeI(668 nm)/HeI(728 nm), to the plasma density. In these calculations, the factors relating the intensity ratio of these lines to the plasma density were taken from the literature on spectral diagnostics.     

Dielectric Dispersion in Ga<Subscript>2</Subscript>S<Subscript>3</Subscript> Thin Films

In this work, the structural, compositional, optical, and dielectric properties of Ga₂S₃ thin films are investigated by means of X-ray diffraction, scanning electron microscopy, energy dispersion X-ray analysis, and ultraviolet—visible light spectrophotometry. The Ga₂S₃ thin films which exhibited amorphous nature in its as grown form are observed to be generally composed of 40.7 % Ga and 59.3 % S atomic content. The direct allowed transitions optical energy bandgap is found to be 2.96 eV. On the other hand, the modeling of the dielectric spectra in the frequency range of 270–1,000 THz, using the modified Drude-Lorentz model for electron-plasmon interactions revealed the electrons scattering time as 1.8 (fs), the electron bounded plasma frequency as ~0.76–0.94 (GHz) and the reduced resonant frequency as 2.20–4.60 ×10¹⁵ (Hz) in the range of 270–753 THz. The corresponding drift mobility of electrons to the terahertz oscillating incident electric field is found to be 7.91 (cm ²/Vs). The values are promising as they nominate the Ga₂S₃ thin films as effective candidates in thin-film transistor and gas sensing technologies.     

Reaction of turbulence at the edge and in the center of the plasma column to pulsed impurity injection caused by the sputtering of the wall coating in L-2M stellarator

Impurity injection into plasma caused by the sputtering of the wall coating in the L-2M stellarator during auxiliary electron cyclotron resonance heating leads to a change in the level of plasma density fluctuations with frequencies above 0.25 MHz: suppression of long-wavelength (k _⊥ = 2 cm^–1) density fluctuations in the edge plasma, intensification of short-wavelength (k _⊥ = 30 cm^–1) and long-wavelength (k _⊥ = 1 cm^–1) fluctuations at the midradius of the plasma column, and intensification of short-wavelength fluctuations (k _⊥ = 20 cm^–1) in the plasma center (including the gyroresonance region). At the same time, the level of fluctuations with frequencies below 0.25 MHz remains unchanged. In the edge plasma, a decrease in the plasma potential and suppression of its fluctuations is observed during impurity injection, which also causes an increase in MHD activity.     

Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential,Intracellular Calcium,and NF-κB in Astroglia

The effects of H₂O₂ are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H₂O₂ concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H₂O₂, using three regimes of exposure – continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity – respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-κB activation. When compared to continuous exposure, fluctuating H₂O₂ induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H₂O₂ provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H₂O₂ induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF-κB activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.     

Group X Secretory Phospholipase A2 Regulates Insulin Secretion through a Cyclooxygenase-2-dependent Mechanism

Group X secretory phospholipase A₂ (GX sPLA₂) potently hydrolyzes membrane phospholipids to release arachidonic acid (AA). While AA is an activator of glucose-stimulated insulin secretion (GSIS), its metabolite prostaglandin E2 (PGE2) is a known inhibitor. In this study, we determined that GX sPLA₂ is expressed in insulin-producing cells of mouse pancreatic islets and investigated its role in beta cell function. GSIS was measured in vivo in wild-type (WT) and GX sPLA₂-deficient (GX KO) mice and ex vivo using pancreatic islets isolated from WT and GX KO mice. GSIS was also assessed in vitro using mouse MIN6 pancreatic beta cells with or without GX sPLA₂ overexpression or exogenous addition. GSIS was significantly higher in islets isolated from GX KO mice compared with islets from WT mice. Conversely, GSIS was lower in MIN6 cells overexpressing GX sPLA₂ (MIN6-GX) compared with control (MIN6-C) cells. PGE2 production was significantly higher in MIN6-GX cells compared with MIN6-C cells and this was associated with significantly reduced cellular cAMP. The effect of GX sPLA₂ on GSIS was abolished when cells were treated with NS398 (a COX-2 inhibitor) or L-798,106 (a PGE2-EP3 receptor antagonist). Consistent with enhanced beta cell function, GX KO mice showed significantly increased plasma insulin levels following glucose challenge and were protected from age-related reductions in GSIS and glucose tolerance compared with WT mice. We conclude that GX sPLA₂ plays a previously unrecognized role in negatively regulating pancreatic insulin secretion by augmenting COX-2-dependent PGE2 production.     

Neutron scattering method for studying bound water in live tissue application to plant leaves

Illustrative neutron scattering studies are presented of rotational modes of bound H₂O in live leaves of Ficus elastica decora, Philodendron cordatum and Peperomia obtusifolia. Interpretation of data is based on the hypothesis that hindrance of rotational motion should result in shifts to higher frequency. Such shifts are verified by comparing scattering from water with that from ice and cytosine monohydrate. Statistical analysis of the scattering from plant leaves is based on the assumption that least-squares fitting can be carried out using variable percentages of ice and water spectra to describe hydrate and free water fractions. It is found that the amount of water present as a 4-fold bonded hydrate is very small, being of the order of a few percent or less.