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Looking at christmas trees in the nucleolus 总被引:2,自引:0,他引:2
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High resolution detection of rRNA and rDNA in plant nucleoli with different activities by in situ hybridization 总被引:2,自引:0,他引:2
Bassy O Jiménez-García LF Echeverría OM Vázquez-Nin GH Díaz de la Espina SM 《Biology of the cell / under the auspices of the European Cell Biology Organization》2000,92(1):59-70
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Isolated nucleoli, nucleolar chromatin, and nucleolar DNA were used as templates for DNA synthesis in appropriately supplemented systems in which RNA polymerases other than RNA polymerase I were blocked by alpha-amanitin. With the aid of nucleotide analysis, DNA-RNA hybridization, and homochromatography fingerprinting, it was found that isolated nucleoli and nucleolar chromatin serve primarily as templates for synthesis of rRNA. However, the products formed with purified nucleolar DNA as a template do not contain the specific rRNA oligonucleotides nor are they appreciably hybridized to the rDNA region on cesium chloride gradients. These results indicate that whole nucleoli and nucleolar chromatin contain control mechanisms that restrict readouts by RNA polymerase I of nucleolar DNA to rDNA. 相似文献
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