| 利用铁葡聚糖分离纤毛虫接合对的方法 |
| |
| 引用本文： | 魏锦瑜,高欣,杨仙玉.利用铁葡聚糖分离纤毛虫接合对的方法[J].动物学杂志,2008,43(2):77-80. |
| |
| 作者姓名： | 魏锦瑜 高欣 杨仙玉 |
| |
| 作者单位： | 浙江林学院林业与生物技术学院,临安,311300 |
| |
| 基金项目： | 国家自然科学基金项目(No.30670297) |
| |
| 摘 要： | 介绍一种制备微小铁葡聚糖颗粒以及利用微小铁葡聚糖颗粒分离纤毛虫接合对的方法,通过本方法可以获得接合率达95%以上,且发育基本同步的实验材料。
|
| 关 键 词： | 纤毛虫 接合对 铁葡聚糖颗粒 |
| 文章编号： | 0250-3263(2008)02-77-04 |
| 收稿时间： | 2007/9/29 0:00:00 |
| 修稿时间： | 2007年9月29日 |
Introducing a Method for Separating Ciliate Conjugating Pairs by Magnetic Iron-dextran Particles |
| |
| WEI Jin-Yu,GAO Xin and YANG Xian-Yu.Introducing a Method for Separating Ciliate Conjugating Pairs by Magnetic Iron-dextran Particles[J].Chinese Journal of Zoology,2008,43(2):77-80. |
| |
| Authors: | WEI Jin-Yu GAO Xin and YANG Xian-Yu |
| |
| Abstract: | In this report,methods for preparing magnetic iron-dextran particles and separating highly synchronized (95%) ciliate conjugating pairs are introduced.When iron-dextran particles are added to the mixtures of complementary mating types of ciliates,single cells and loose conjugating pairs form food vacuoles containing the iron-dextran particles while absent from the tight mating pairs.By applying magnetic force,single cells and loose mating pairs can be removed from the tight conjugating pairs.This method has several advantages: simplicity,speediness,high quality,universality and practicality.No negative effects have been found when perform the experiments by this method. |
| |
| Keywords: | Ciliate Conjugating pairs Iron-dextran particles |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《动物学杂志》浏览原始摘要信息 |
|
点击此处可从《动物学杂志》下载全文 |
|
