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两种蛇消化道5-羟色胺细胞的免疫组织化学定位及比较
引用本文:张志强,张盛周,吴孝兵.两种蛇消化道5-羟色胺细胞的免疫组织化学定位及比较[J].动物学杂志,2001,36(6):11-14.
作者姓名:张志强  张盛周  吴孝兵
作者单位:安徽师范大学生命科学学院,
基金项目:安徽省教育厅科研项目,安徽省跨世纪学术与技术带头人基金,安徽师范大学校科研和教改项目,96JL0036,2001kj099,,,,,
摘    要:应用5-羟色胺特异性抗血清,对眼镜蛇和乌梢蛇消化道含5-HT的内分泌细胞进行了免疫组织化学定位和比较。5-HT细胞的形态在两种蛇中大致相同,消化道各部位都有呈圆形和长梭形(具胞突)的阳性细胞。食道和胃以圆形细胞为主,肠道段则以长梭形细胞为主。眼镜蛇5-HT细胞以十二指肠密度为最高,食道最低;乌梢蛇以幽门部密度为最高,直肠最低。肠道段两者分布特点相同,从十二指肠至直肠分布密度渐疏。本文还对两种蛇5-HT细胞分布型的原因、形态和功能相适应的特点进行了讨论。

关 键 词:  消化道  5-HT细胞  免疫组织化学定位
文章编号:0250-3263(2001)06-11-04

Immunohistochemical Localization and Comparison on 5-HT Cells in the Digestive Tract of Snakes
ZHANG Zhi-Qiang ZHANG Sheng-Zhou WU Xiao-Bing.Immunohistochemical Localization and Comparison on 5-HT Cells in the Digestive Tract of Snakes[J].Chinese Journal of Zoology,2001,36(6):11-14.
Authors:ZHANG Zhi-Qiang ZHANG Sheng-Zhou WU Xiao-Bing
Abstract:The presence of 5-HT cells had been investigated and compared by the anti-5-HT antiserum in the digestive tract of Naja naja and Zaocys dhumnades. Mucosa endocrine cells reacting specifically with the antisera to 5-HT were detectable throughout the digestive tract. In general, the morphology of these cells was similar to the mammalian pattern. Oval cells were mainly in the oesophagus and rectum. The cells which had cell processes were most present in the stomach and small intestine. The distribution of 5-HT cells in Naja naja was observed with the highest density in the duodenum and rarely in the oesophagus. A great number of 5-HT cells in Zaocys dhumnades were detected in the stamachus pyloricus, rarely in the rectum. In the small intestine and rectum, the distributive density of 5-HT cells in Naja naja was similar to that of 5-HT cells in Zaocys dhymnades, namely, decreased from duodenum to rectum. Considering the feeding habit and life environment, the distributive mode of 5-HT cells was discussed, which Naja naja and Zaocys dhumnades were involved.
Keywords:Snakes  Digestive tract  5-HT cells  Immunohistochemistry
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