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对胸鳍棘鉴定鱼类年龄方法的技术改进——简易脱钙切片法
引用本文:陈康贵,王德寿,王瑞兰.对胸鳍棘鉴定鱼类年龄方法的技术改进——简易脱钙切片法[J].动物学杂志,2002,37(5):46-48.
作者姓名:陈康贵  王德寿  王瑞兰
作者单位:1. 湘潭师范学院生命科学系,湖南湘潭,411201
2. 西南师范大学生命科学学院,重庆,400715
摘    要:用大鳍Hu、粗唇Wei、瓦氏黄颡鱼等的胸鳍棘作为实验材料,用1%-5%的硝酸脱钙切片后,鉴定年龄。为适应大规模的年龄鉴定,对常规的组织切片步骤和时间进行了简化、缩短。与传统的锯片法及磨片法相比,该方法易于操作,有助于准确的年龄鉴定、年轮半径测量和生长推算,特别适用于中、小型无鳞鱼类的年龄鉴定。

关 键 词:胸鳍棘  鉴定  鱼类年龄  技术改进  简易脱钙切片法
文章编号:0250-3263(2002)05-46-03

A New Method for Aging of Fish Using Simplified Histological Sections of the Pectoral Fin after Decalcification
Abstract:The pectoral fins of Mystus macropterus, Leioacssis crassilabris, and Pelteobagrus vachelli etc., were used to age these bagrid catfishes. The collected pectoral fins were sectioned after decalcification. The normal histological section method was modified with some steps omitted and some shortened so as to be able to age large numbers of fish with reasonable efficiency. Compared with traditional sections made by cutting and grinding without decalcification, this method is much more easy to perform and much more accurate with respect to annulus radius measurement and growth calculation, and therefore, is especially applicable to the aging of medium and small sized scale less fish.
Keywords:Aging of fish  Pectoral fin  Decalcification  Histological sections
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