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斑马鱼鳃的光镜和透射电镜观察
引用本文:赵巧雅,王新栋,孙雪婧,王玲玲,王涛只,陈秋生,林金杏.斑马鱼鳃的光镜和透射电镜观察[J].动物学杂志,2018,53(1):92-98.
作者姓名:赵巧雅  王新栋  孙雪婧  王玲玲  王涛只  陈秋生  林金杏
作者单位:1. 南京农业大学动物医学院 南京210095;2. 上海实验动物研究中心 上海201203
基金项目:上海市科委科研计划项目(No. 16140904300和No.15140901000);
摘    要:应用光学显微镜和透射电镜对斑马鱼(Danio rerio)鳃的组织结构及鳃丝、鳃小片超微结构进行了观察。结果表明,斑马鱼有4对全鳃,鳃耙呈长锥状,鳃丝呈梳状排列在鳃弓上,鳃小片均匀排列在鳃丝两侧。鳃小片由上皮细胞、柱细胞、内皮细胞和毛细血管网组成,鳃小片基部和血管周围分布有泌氯细胞,胞内有丰富的线粒体和排泄小泡,根据线粒体形态特征和细胞质电子密度可将其分为两个亚型。黏液细胞通常与泌氯细胞对生存在,并且有通外的开口。斑马鱼鳃组织结构与其他硬骨鱼鳃结构相似,其结构和功能有密切的关系。

关 键 词:斑马鱼    显微结构  超微结构  Zebrafish  Danio  rerio  Gill  Microstructure  Ultrastructure
收稿时间:2017/5/6 0:00:00
修稿时间:2018/1/2 0:00:00

Light and Transmission Electron Microscopic Observation on the Structure of Zebrafish (Danio rerio) Gill
ZHAO Qiao-Y,CHEN Qiu-Sheng.Light and Transmission Electron Microscopic Observation on the Structure of Zebrafish (Danio rerio) Gill[J].Chinese Journal of Zoology,2018,53(1):92-98.
Authors:ZHAO Qiao-Y  CHEN Qiu-Sheng
Abstract:Optical microscope and transmission electron microscope was used to investigate the microstructure and ultrastructure of gill and gill lamellae in Zebrafish (Danio rerio). The results showed that there were four pairs of holobranch, gill rakers seems to be prick-shaped, Primary lamellas are arranged in a comb on the gill arch, secondary lamellas are evenly arranged on both sides of the primary lamella (Fig. 1). Secondary lamella consist of pillar cells, epithelial cells, endothelial cells and capillary network. Chloride cell is distributed mainly over the base of primary lamellae and perivascular, intracellular abundant mitochondria and excretory vesicle, which can be divided into two subtypes according to the morphological characteristics and the electron density. Mucus cells usually correspond to chloride cells, and they often seemed to be open at the surface between adjacent epithelial cells. the The gill structure is similar with other teleost fish gill structure, which is closely related to its function (Fig. 2).
Keywords:Zebrafish  Danio Rerio  Gill  Microstructure  Ultrastructure
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