首页 | 官方网站   微博 | 高级检索  
     

温度和紫外辐射胁迫对西藏飞蝗抗氧化系统的影响
引用本文:李庆,吴蕾,杨刚,匡健康,封传红,罗怀海,杨群芳,蒋春先,王海建.温度和紫外辐射胁迫对西藏飞蝗抗氧化系统的影响[J].生态学报,2012,32(10):3189-3197.
作者姓名:李庆  吴蕾  杨刚  匡健康  封传红  罗怀海  杨群芳  蒋春先  王海建
作者单位:1. 四川农业大学农学院,成都温江,611130
2. 四川甘孜州农业局 626000
3. 四川省农业厅植保站,成都,610041
基金项目:四川省教育厅重点项目(07ZA066)
摘    要:为探讨西藏飞蝗(Locusta migratoria tibetensis Chen)对环境的适应机制,报道了温度和紫外辐射胁迫对西藏飞蝗抗氧化系统影响。以西藏飞蝗成虫为试材,研究5—20℃低温、30—45℃高温胁迫和紫外线辐射对其抗氧化酶活性和膜脂过氧化物丙二醛(MDA)含量的影响。在5—20℃低温胁迫下,成虫体壁和消化道超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量分别随温度降低而升高;在30—35℃高温胁迫下,成虫SOD、POD和CAT活性分别随温度升高而升高,当温度超过35℃时,3种氧化酶活性均下降。长波紫外辐射(UV-A)和中波紫外辐射(UV-B)对处理后24h和72h成虫的SOD活性的影响大于可见光,在UV-A、UV-B和可见光3种光波长处理下,成虫的POD和CAT活性随光照时间的延长而增加,其中UV-B对两种酶活性影响大于UV-A和可见光,表现为UV-B处理组>UV-A处理组>可见光处理组;UV-A和UV-B处理能导致虫体体壁MDA含量明显升高,且对脂质过氧化的诱导存在时间效应;雌虫体壁、雌虫消化道、雄虫体壁和雄虫消化道的MDA含量分别在UV-B72h、UV-A72h、UV-A72h和UV-B72h达最大值0.72、0.88、0.66和0.94 nmol/g鲜重。在20—5℃低温胁迫下,西藏飞蝗成虫抗氧化酶活性升高,能较好的保护自身免遭活性氧自由基的伤害;西藏飞蝗对高温忍耐力差,在高于35℃高温胁迫下,成虫SOD、POD、CAT活性均下降;在长波和中波紫外辐射下,西藏飞蝗抗氧化酶活性显著升高,是西藏飞蝗对紫外线辐射强度大的青藏高原的一种重要适应。

关 键 词:西藏飞蝗  抗氧化酶  膜脂过氧化  环境胁迫
收稿时间:4/26/2011 4:16:41 PM
修稿时间:3/25/2012 7:06:12 PM

Effects of temperature stress and ultraviolet radiation stress on antioxidant systems of Locusta migratoria tibetensis Chen
LI Qing,WU Lei,YANG Gang,KUAN Jiankang,FENG Chuanhong,LUO Huaihai,YANG Quanfang,JANG Chunxian and WANG Haijian.Effects of temperature stress and ultraviolet radiation stress on antioxidant systems of Locusta migratoria tibetensis Chen[J].Acta Ecologica Sinica,2012,32(10):3189-3197.
Authors:LI Qing  WU Lei  YANG Gang  KUAN Jiankang  FENG Chuanhong  LUO Huaihai  YANG Quanfang  JANG Chunxian and WANG Haijian
Affiliation:Sichuan Agricultural University,Sichuan Agricultural University,,,,,,,
Abstract:Mechanisms of adaptation to environmental stress in the pest species Locusta migratoria tibetensis Chen were investigated with respect to the effects of temperature and ultraviolet(UV) radiation on its antioxidant system.The effects of low temperature(5—20 ℃),high temperatures(30—45 ℃),and UV radiation on the activities of antioxidant enzymes and on the malondialdehyde(MDA) content of L.migratoria tibetensis adults were measured.At low temperatures,decreasing from 20 ℃ to 5 ℃,the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT),and the MDA contents of the adult integument and alimentary canal all increased.The activities of SOD,POD and CAT also increased at high temperatures,increasing from 30 ℃ to 35 ℃.However,all enzyme activities decreased significantly when the temperature exceeded 35 ℃.The effects of radiation were examined at three wavelengths,ultraviolet-A(UV-A),ultraviolet-B(UV-B),and visible light.POD and CAT activities increased progressively during increasing exposure to radiation at all three wavelengths(the magnitudes of the changes were in the order: UV-B treatment > UV-A treatment > visible light treatment).UV-A and UV-B produced greater changes in the SOD activity of adult locusts than did visible light,during 24h and 72h exposures.Both UV-A and UV-B radiation caused a marked increase in the MDA content of the body wall,indicating a time-dependent induction of lipid peroxidation.Maximal contents of MDA were 0.72 nmol/g FW in the female integument,0.88 nmol/g FW in the female alimentary canal,0.66 nmol/g FW in male integument and 0.94 nmol/g FW in the male alimentary canal after exposures of 72 h UV-B,72 h UV-A,72 h UV-A,and 72 h UV-B,respectively.Thus,adult L.migratoria tibetensis are able to endure low temperatures(5—20 ℃) by protecting themselves from damage by reactive oxygen species but are unable to endure temperatures exceeding 35 ℃,which disturb the insects′ antioxidant enzyme system.The increased activities of antioxidant enzymes of L.migratoria tibetensis under UV-A and UV-B stress represent important adaptations to the environment of the Qinghai-Tibet Plateau,which is characterized by intense ultraviolet irradiation.
Keywords:??Locusta migratoria tibetensis??Antioxidant enzymes??Lipid peroxidation  Environment Stress
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《生态学报》浏览原始摘要信息
点击此处可从《生态学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号